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The map label for this gene is gluQ [H]

Identifier: 83313411

GI number: 83313411

Start: 4717363

End: 4718196

Strand: Reverse

Name: gluQ [H]

Synonym: amb4312

Alternate gene names: 83313411

Gene position: 4718196-4717363 (Counterclockwise)

Preceding gene: 83313414

Following gene: 83313407

Centisome position: 94.99

GC content: 70.38

Gene sequence:

>834_bases
GTGACCATCACCCGCTTCGCCCCCAGTCCCACCGGGGAACTGCATCTGGGCCACGCCCATTCCGCCCTGTTCGCCTTTGC
CGCGGCAAAGGCGGCGGGGGGGCGCTTTCTTTTGCGCATCGAGGACATCGACCGCACCCGCTGTCGCCCCGAGTTCACTC
AAGGGATCTTCACCGATCTCGGCTGGCTGGGGCTGGACTGGGAACGGCCCGTGCGCGTCCAGTCGGAGCATTTCGACGAA
TACCGCGCCGCGCTGGACCGGCTGGAGGGCATGGGGCTGCTCTATCCCTGCTTTTGCACCCGCAAGGATATCGCCCGCGA
GGTCGAGGGTGCCGGCCATGCGCCGCACGGCCCCGATGGGCCGCTTTATCCCGGCCATTGCCGCCATCTGTCGGTGGCCG
AACGGCGAGAGCGGCGCGAGGCGGGCCAGGCCTTTGCGTTGCGCCTGGACGTGGCGGCGGCGGCGGCGCGGGTCGGTCCC
CTGGCCTGGCATGACCGGGCGCAGGGAGAGCAGACCGCCCAGCCTGAACTGTTCGGCGACGTGGTGCTGGCCCGCAAGGA
CATTCCGGCCAGCTACCATCTGGCGGTAACCGTCGACGACGCCCTGCAGGGGGTCACGCTGGTGACCCGAGGCCAGGACC
TGTTCGAGGCCACCCATGTGCACCGGCTGTTGCAGGCGCTGCTGGGCCTGCCGGTGCCGGGCTACCTCCATCACCGCCTG
CTCACCGACCCGAGCGGGCGGCGTTATGCCAAGCGTGACCGCTCGCTGACGCTGAGGGCCCTGCGCGAGGCGGGCCGCAC
CCCGGACGAGGTGCGGTCCCTGGCGGGATTTTGA

Upstream 100 bases:

>100_bases
TGATGGGGGACGGACTGGGCATGATGGCTCCTGACGATACGATCTGGAGGCATTATGGTATGGTTCGCCGCCCCTGTCCC
GCCAAAGCTCGCGACATATT

Downstream 100 bases:

>100_bases
CCAAGCCCGCTTCGCGGCAGGGCACCCACCCTGGCCCGTTCGGAGACACAGCCTCCAACCCTCCAGGCTTTAAAAAACTG
AAGGGTCCGGGACACTGTGC

Product: glutamyl- and glutaminyl-tRNA synthetase

Products: AMP; L-Glutamyl-tRNA(Glu); Diphosphate [C]

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTITRFAPSPTGELHLGHAHSALFAFAAAKAAGGRFLLRIEDIDRTRCRPEFTQGIFTDLGWLGLDWERPVRVQSEHFDE
YRAALDRLEGMGLLYPCFCTRKDIAREVEGAGHAPHGPDGPLYPGHCRHLSVAERRERREAGQAFALRLDVAAAAARVGP
LAWHDRAQGEQTAQPELFGDVVLARKDIPASYHLAVTVDDALQGVTLVTRGQDLFEATHVHRLLQALLGLPVPGYLHHRL
LTDPSGRRYAKRDRSLTLRALREAGRTPDEVRSLAGF

Sequences:

>Translated_277_residues
MTITRFAPSPTGELHLGHAHSALFAFAAAKAAGGRFLLRIEDIDRTRCRPEFTQGIFTDLGWLGLDWERPVRVQSEHFDE
YRAALDRLEGMGLLYPCFCTRKDIAREVEGAGHAPHGPDGPLYPGHCRHLSVAERRERREAGQAFALRLDVAAAAARVGP
LAWHDRAQGEQTAQPELFGDVVLARKDIPASYHLAVTVDDALQGVTLVTRGQDLFEATHVHRLLQALLGLPVPGYLHHRL
LTDPSGRRYAKRDRSLTLRALREAGRTPDEVRSLAGF
>Mature_276_residues
TITRFAPSPTGELHLGHAHSALFAFAAAKAAGGRFLLRIEDIDRTRCRPEFTQGIFTDLGWLGLDWERPVRVQSEHFDEY
RAALDRLEGMGLLYPCFCTRKDIAREVEGAGHAPHGPDGPLYPGHCRHLSVAERRERREAGQAFALRLDVAAAAARVGPL
AWHDRAQGEQTAQPELFGDVVLARKDIPASYHLAVTVDDALQGVTLVTRGQDLFEATHVHRLLQALLGLPVPGYLHHRLL
TDPSGRRYAKRDRSLTLRALREAGRTPDEVRSLAGF

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=282, Percent_Identity=31.5602836879433, Blast_Score=117, Evalue=9e-27,
Organism=Escherichia coli, GI1788743, Length=268, Percent_Identity=31.3432835820896, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI226510925, Length=262, Percent_Identity=36.2595419847328, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1786895, Length=118, Percent_Identity=36.4406779661017, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71993394, Length=283, Percent_Identity=30.7420494699647, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6324540, Length=273, Percent_Identity=30.4029304029304, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324742, Length=128, Percent_Identity=34.375, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24665321, Length=280, Percent_Identity=33.5714285714286, Blast_Score=133, Evalue=1e-31,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: 6.1.1.17 [C]

Molecular weight: Translated: 30726; Mature: 30595

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTITRFAPSPTGELHLGHAHSALFAFAAAKAAGGRFLLRIEDIDRTRCRPEFTQGIFTDL
CCEECCCCCCCCCEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHH
GWLGLDWERPVRVQSEHFDEYRAALDRLEGMGLLYPCFCTRKDIAREVEGAGHAPHGPDG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHCHHHHHHHHHCCCCCCCCCCC
PLYPGHCRHLSVAERRERREAGQAFALRLDVAAAAARVGPLAWHDRAQGEQTAQPELFGD
CCCCCCCCCCHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCHHCCCCCCCCCCCCHHHHH
VVLARKDIPASYHLAVTVDDALQGVTLVTRGQDLFEATHVHRLLQALLGLPVPGYLHHRL
HHHHHCCCCCCEEEEEEECHHHCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LTDPSGRRYAKRDRSLTLRALREAGRTPDEVRSLAGF
CCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCC
>Mature Secondary Structure 
TITRFAPSPTGELHLGHAHSALFAFAAAKAAGGRFLLRIEDIDRTRCRPEFTQGIFTDL
CEECCCCCCCCCEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHH
GWLGLDWERPVRVQSEHFDEYRAALDRLEGMGLLYPCFCTRKDIAREVEGAGHAPHGPDG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHCHHHHHHHHHCCCCCCCCCCC
PLYPGHCRHLSVAERRERREAGQAFALRLDVAAAAARVGPLAWHDRAQGEQTAQPELFGD
CCCCCCCCCCHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCHHCCCCCCCCCCCCHHHHH
VVLARKDIPASYHLAVTVDDALQGVTLVTRGQDLFEATHVHRLLQALLGLPVPGYLHHRL
HHHHHCCCCCCEEEEEEECHHHCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LTDPSGRRYAKRDRSLTLRALREAGRTPDEVRSLAGF
CCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-Glutamate; tRNA (Glu) [C]

Specific reaction: ATP + L-Glutamate + tRNA (Glu) --> AMP + L-Glutamyl-tRNA(Glu) + Diphosphate [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA