Click here to switch to the map view.

The map label for this gene is rsmD [H]

Identifier: 83313224

GI number: 83313224

Start: 4529747

End: 4530301

Strand: Reverse

Name: rsmD [H]

Synonym: amb4125

Alternate gene names: 83313224

Gene position: 4530301-4529747 (Counterclockwise)

Preceding gene: 83313225

Following gene: 83313223

Centisome position: 91.21

GC content: 72.07

Gene sequence:

>555_bases
ATGCGCATCGTCGCCGGTCTTCATAAGGGCCGCCGCCTGGAGGCCCCTTCGGGCCGCGACACCCGCCCCACCGCCGATCG
GGTGCGCCAGGCGCTGTTCGACATCCTGATGCATTCCGATCTGGTGGAGATGGAGGGCGCCCGCGTGGTGGACGCCTTCG
CCGGATCGGGGGCGCTGGGCCTGGAGGCCCTGTCGCGCGGCGCGGCCCATGCCTGCTTCCTGGAACTGGCGGCGCAACCC
CTGGCCGCCATCTACGCCAACGTCAAGGCTCTGAAGGTGGAGGGCGAGAGCAAGGTCCTGCGCGCCGACGCCGCCAAGCC
GCCTGCCGCGCCCCACGCCTGCACCCTGGCTTTTCTCGACCCGCCCTATCATTCCGGCCTCGCCGGGCCGTGCCTCGAAG
GTCTGGCCGCCAAGGGCTGGCTGGAAGGCGGCGCCCTGGCAGTGGTGGAAGTGGCCGCCGACGAGGCCTTTGCGCCTCCG
CCCGGTTTCGATGCGGTGGACGAGCGCCCGCGTGGCGCGGCACGGCTGGTGTTCGTGGTTTACCGCCGCCCGTGA

Upstream 100 bases:

>100_bases
GTCGCCGCCAAGGCCCGCAAGCAGAAGCTGGCCGAGGACAAGCGCGCCGCGAGCGCCAAAAAAGACAAGCCCTACGAGGC
CCGGCAAGCGGGCAAGCCCG

Downstream 100 bases:

>100_bases
TGCGTGCTACAAGGCGACTGTTCCCGTAATGTTCGGATTGCCGCCATGGATTTTGCCCTGATCTCCGCCGCTCTTTCCCT
GCTTGCCCTGATCGGGGTGG

Product: N6-adenine-specific methylase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MRIVAGLHKGRRLEAPSGRDTRPTADRVRQALFDILMHSDLVEMEGARVVDAFAGSGALGLEALSRGAAHACFLELAAQP
LAAIYANVKALKVEGESKVLRADAAKPPAAPHACTLAFLDPPYHSGLAGPCLEGLAAKGWLEGGALAVVEVAADEAFAPP
PGFDAVDERPRGAARLVFVVYRRP

Sequences:

>Translated_184_residues
MRIVAGLHKGRRLEAPSGRDTRPTADRVRQALFDILMHSDLVEMEGARVVDAFAGSGALGLEALSRGAAHACFLELAAQP
LAAIYANVKALKVEGESKVLRADAAKPPAAPHACTLAFLDPPYHSGLAGPCLEGLAAKGWLEGGALAVVEVAADEAFAPP
PGFDAVDERPRGAARLVFVVYRRP
>Mature_184_residues
MRIVAGLHKGRRLEAPSGRDTRPTADRVRQALFDILMHSDLVEMEGARVVDAFAGSGALGLEALSRGAAHACFLELAAQP
LAAIYANVKALKVEGESKVLRADAAKPPAAPHACTLAFLDPPYHSGLAGPCLEGLAAKGWLEGGALAVVEVAADEAFAPP
PGFDAVDERPRGAARLVFVVYRRP

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=186, Percent_Identity=34.4086021505376, Blast_Score=96, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 19283; Mature: 19283

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIVAGLHKGRRLEAPSGRDTRPTADRVRQALFDILMHSDLVEMEGARVVDAFAGSGALG
CCEECCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCEEEEHHCCCCCHH
LEALSRGAAHACFLELAAQPLAAIYANVKALKVEGESKVLRADAAKPPAAPHACTLAFLD
HHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEECCCCCCCCCCCEEEEEEEC
PPYHSGLAGPCLEGLAAKGWLEGGALAVVEVAADEAFAPPPGFDAVDERPRGAARLVFVV
CCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEE
YRRP
EECC
>Mature Secondary Structure
MRIVAGLHKGRRLEAPSGRDTRPTADRVRQALFDILMHSDLVEMEGARVVDAFAGSGALG
CCEECCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCEEEEHHCCCCCHH
LEALSRGAAHACFLELAAQPLAAIYANVKALKVEGESKVLRADAAKPPAAPHACTLAFLD
HHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEECCCCCCCCCCCEEEEEEEC
PPYHSGLAGPCLEGLAAKGWLEGGALAVVEVAADEAFAPPPGFDAVDERPRGAARLVFVV
CCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEE
YRRP
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]