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The map label for this gene is trmL [H]

Identifier: 83313186

GI number: 83313186

Start: 4488559

End: 4488894

Strand: Reverse

Name: trmL [H]

Synonym: amb4087

Alternate gene names: 83313186

Gene position: 4488894-4488559 (Counterclockwise)

Preceding gene: 83313200

Following gene: 83313185

Centisome position: 90.37

GC content: 68.45

Gene sequence:

>336_bases
ATGGATTATATTCACCAGGCCAGTTACACCCGCCATACCTCGTGGGAGGTCTTCGCCGCCACCCTGGCCGCCCAGGGGCG
CCGGCTGGTGCTGCTGACCACCGGCGGCGCCGTGCGTCATGACCGCTTTCCTTTCGCGCCCGGGGACACCATCATGGTGG
GACGGGAAAGCTGCGGCGTGCCGCCAGACGTGGTCGATCGGGCCGAGGCGCGCATCCGCATTCCCATGCAGGTGGGGGCC
CGGTCGCTCAATGTGGCGCTGGCCGCCGCCATCGCCACCGCCGAGGCCCTGAGACGCCTGGACGGATTTCCGCAAGAAGG
GAGTGAGGACGCATGA

Upstream 100 bases:

>100_bases
CGGGGCGCTGCTGCGTCTGTCCGCCTGCCTGGGCGTGCCGCTGGATATAGTCGAACCCTGCGGCTTTGTCCTTGATGAAC
GTAAAGTCCGCCGGGCCGGA

Downstream 100 bases:

>100_bases
GCCGCGATACCGTCAAGCACTGGGACGATGCCCGCGCCGCGGCCTATGACAAGCGGGTGCGCGAAGCCATTCCCGGCTAT
GACGCCCTGCATGTCCTGTC

Product: rRNA methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 111; Mature: 111

Protein sequence:

>111_residues
MDYIHQASYTRHTSWEVFAATLAAQGRRLVLLTTGGAVRHDRFPFAPGDTIMVGRESCGVPPDVVDRAEARIRIPMQVGA
RSLNVALAAAIATAEALRRLDGFPQEGSEDA

Sequences:

>Translated_111_residues
MDYIHQASYTRHTSWEVFAATLAAQGRRLVLLTTGGAVRHDRFPFAPGDTIMVGRESCGVPPDVVDRAEARIRIPMQVGA
RSLNVALAAAIATAEALRRLDGFPQEGSEDA
>Mature_111_residues
MDYIHQASYTRHTSWEVFAATLAAQGRRLVLLTTGGAVRHDRFPFAPGDTIMVGRESCGVPPDVVDRAEARIRIPMQVGA
RSLNVALAAAIATAEALRRLDGFPQEGSEDA

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=106, Percent_Identity=36.7924528301887, Blast_Score=64, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 11991; Mature: 11991

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYIHQASYTRHTSWEVFAATLAAQGRRLVLLTTGGAVRHDRFPFAPGDTIMVGRESCGV
CCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCEEEEECCCCCC
PPDVVDRAEARIRIPMQVGARSLNVALAAAIATAEALRRLDGFPQEGSEDA
CHHHHCCCCCEEECHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MDYIHQASYTRHTSWEVFAATLAAQGRRLVLLTTGGAVRHDRFPFAPGDTIMVGRESCGV
CCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCEEEEECCCCCC
PPDVVDRAEARIRIPMQVGARSLNVALAAAIATAEALRRLDGFPQEGSEDA
CHHHHCCCCCEEECHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA