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The map label for this gene is nusA

Identifier: 83313172

GI number: 83313172

Start: 4474471

End: 4475982

Strand: Reverse

Name: nusA

Synonym: amb4073

Alternate gene names: 83313172

Gene position: 4475982-4474471 (Counterclockwise)

Preceding gene: 83313173

Following gene: 83313171

Centisome position: 90.11

GC content: 65.34

Gene sequence:

>1512_bases
ATGGAACGGATCGCCGCACTGCCCCGCCCCGAACTCCTTCAGGTGGCCGACGCCGTCGCCCGCGACAAGGGCATCGACCG
CGACGAGGTTCTCGAAGCCATGGAGCAGGCCATTCAGAAGGCCGGTCGCTCCAAGTATGGCCACGAGCACGACATTCGCG
CCCATATCGATCGCAAGACCGGTGAAATCCAGCTGGCCCGCTATATCGAAGTGGTCGAGGAGGTGGAGAACGAGGCCACC
CAGCAGACCCTGAAGCAGGTGCTGAAGAAGAAGCCCGACGCCGTCGTCGGCGACTTCCTGGTCGATCCGCTGCCGCCCAT
CGATTTCGGCCGCATTGCCGCCCAGACCGCCAAGCAGGTCATCGTCCAGAAGGTGCGCGATGCCGAGCGTCAGCGCCAGT
TCAACGAATACAAGGACCGCGTCGGCGAGATCAGCAATGGTCTGGTCAAGCGCGTCGAGTTCGGCAACGTCATCGTCGAT
CTGGGCCGGGCCGAGGCGCTGCTGCGCCGCGACGAACTGATCCCGCGCGAGACCTTCCGCACCGGCGACCGCGTCCGCGC
CTATATCTACGATGTCCGCCAGGAGCCCCGGGGGCCGCAGATCTTCCTGTCGCGCACCCATCCCATCTTCATGGCCAAGC
TGTTCGCCCAGGAAGTGCCGGAAATCTATGACGGCATCATCGAGATCAAGGCGGTGGCCCGTGACCCGGGCTCGCGCGCC
AAGATCGCCGTGCTGTCCCACGATTCCTCCATCGACCCCGTCGGCGCCTGCGTCGGCATGCGCGGCTCGCGCGTGCAGGC
TGTGGTGGCCGAGCTGCAGGGCGAGAAGATCGACATCATCCAGTGGTCGCCCGACGTCGCCACCTTCGTGGTCAACGGCC
TGGCCCCCGCCGAGGTCACCAAGGTGGTGCTGGACGAGGAAGCCGGCCGCATCGAGGTGGTGGTGCCCGACGACCAGCTG
TCTCTCGCCATCGGCCGTCGCGGCCAGAATGTGCGTCTGGCCAGCCAGCTGACCCAGTGGAACATCGACATCCTCACCGA
GGCCGAGGAATCCGAGCGCCGCACCGAGGAATTCCGTTCGCGTTCGAATATGTTCATCCAGGCCCTGGACGTTGACGACG
TCATCGCCCATCTGCTGGTGACCGAGGGCTTCTCGTCGGTGGAGGAGGTGGCCTACGTGCCGGCCGAGGACATCGCCGAC
ATCGAGGGCTTCGACGAGAACGTCGCCGAGGAACTGCAGACCCGGGCTCGCAACTTCCTGGCCGAGCAGGACGAGCGCTA
CGACCAGATGCGCAAGGACATGGGCGTCGCCGACGAGATGGCCGCCATCGAGGGGCTGGCTCCGTCCATGCTGGTCAAGC
TGGGCGACAAGGGCATCAAGACCCTGGACGACCTGGGCGATCTGGCCGGTGACGAGCTGATCGACATCCTGGGCAAGGAC
GCCATGACCGAGGACGAGGCCAATGCCGTGATCATGGCGGCCCGTGCTCATTGGTTCGAGGAAGGCAAGTAA

Upstream 100 bases:

>100_bases
CATTCCGCTGGCTGATGTTCGCGGTGCCAAGCTGGTGCTGACCGACGAACTGATCGCCGCGACGTTGAAAGACCAGGAAG
AGTGAGGACGAAGTAGGACC

Downstream 100 bases:

>100_bases
GGGGAGCGGCATGGCCGAATCCCCCGACTTGGACGAAGAAGAAGAGGTCGGACCCGAACGCCGGTGCATCGTCACCGGTC
AGGTCCGACCCAAGTCGGAA

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MERIAALPRPELLQVADAVARDKGIDRDEVLEAMEQAIQKAGRSKYGHEHDIRAHIDRKTGEIQLARYIEVVEEVENEAT
QQTLKQVLKKKPDAVVGDFLVDPLPPIDFGRIAAQTAKQVIVQKVRDAERQRQFNEYKDRVGEISNGLVKRVEFGNVIVD
LGRAEALLRRDELIPRETFRTGDRVRAYIYDVRQEPRGPQIFLSRTHPIFMAKLFAQEVPEIYDGIIEIKAVARDPGSRA
KIAVLSHDSSIDPVGACVGMRGSRVQAVVAELQGEKIDIIQWSPDVATFVVNGLAPAEVTKVVLDEEAGRIEVVVPDDQL
SLAIGRRGQNVRLASQLTQWNIDILTEAEESERRTEEFRSRSNMFIQALDVDDVIAHLLVTEGFSSVEEVAYVPAEDIAD
IEGFDENVAEELQTRARNFLAEQDERYDQMRKDMGVADEMAAIEGLAPSMLVKLGDKGIKTLDDLGDLAGDELIDILGKD
AMTEDEANAVIMAARAHWFEEGK

Sequences:

>Translated_503_residues
MERIAALPRPELLQVADAVARDKGIDRDEVLEAMEQAIQKAGRSKYGHEHDIRAHIDRKTGEIQLARYIEVVEEVENEAT
QQTLKQVLKKKPDAVVGDFLVDPLPPIDFGRIAAQTAKQVIVQKVRDAERQRQFNEYKDRVGEISNGLVKRVEFGNVIVD
LGRAEALLRRDELIPRETFRTGDRVRAYIYDVRQEPRGPQIFLSRTHPIFMAKLFAQEVPEIYDGIIEIKAVARDPGSRA
KIAVLSHDSSIDPVGACVGMRGSRVQAVVAELQGEKIDIIQWSPDVATFVVNGLAPAEVTKVVLDEEAGRIEVVVPDDQL
SLAIGRRGQNVRLASQLTQWNIDILTEAEESERRTEEFRSRSNMFIQALDVDDVIAHLLVTEGFSSVEEVAYVPAEDIAD
IEGFDENVAEELQTRARNFLAEQDERYDQMRKDMGVADEMAAIEGLAPSMLVKLGDKGIKTLDDLGDLAGDELIDILGKD
AMTEDEANAVIMAARAHWFEEGK
>Mature_503_residues
MERIAALPRPELLQVADAVARDKGIDRDEVLEAMEQAIQKAGRSKYGHEHDIRAHIDRKTGEIQLARYIEVVEEVENEAT
QQTLKQVLKKKPDAVVGDFLVDPLPPIDFGRIAAQTAKQVIVQKVRDAERQRQFNEYKDRVGEISNGLVKRVEFGNVIVD
LGRAEALLRRDELIPRETFRTGDRVRAYIYDVRQEPRGPQIFLSRTHPIFMAKLFAQEVPEIYDGIIEIKAVARDPGSRA
KIAVLSHDSSIDPVGACVGMRGSRVQAVVAELQGEKIDIIQWSPDVATFVVNGLAPAEVTKVVLDEEAGRIEVVVPDDQL
SLAIGRRGQNVRLASQLTQWNIDILTEAEESERRTEEFRSRSNMFIQALDVDDVIAHLLVTEGFSSVEEVAYVPAEDIAD
IEGFDENVAEELQTRARNFLAEQDERYDQMRKDMGVADEMAAIEGLAPSMLVKLGDKGIKTLDDLGDLAGDELIDILGKD
AMTEDEANAVIMAARAHWFEEGK

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=491, Percent_Identity=45.010183299389, Blast_Score=410, Evalue=1e-116,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 56181; Mature: 56181

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERIAALPRPELLQVADAVARDKGIDRDEVLEAMEQAIQKAGRSKYGHEHDIRAHIDRKT
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCC
GEIQLARYIEVVEEVENEATQQTLKQVLKKKPDAVVGDFLVDPLPPIDFGRIAAQTAKQV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHH
IVQKVRDAERQRQFNEYKDRVGEISNGLVKRVEFGNVIVDLGRAEALLRRDELIPRETFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECHHHHHHHHHHHCCCHHHHC
TGDRVRAYIYDVRQEPRGPQIFLSRTHPIFMAKLFAQEVPEIYDGIIEIKAVARDPGSRA
CCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KIAVLSHDSSIDPVGACVGMRGSRVQAVVAELQGEKIDIIQWSPDVATFVVNGLAPAEVT
EEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCHHHHH
KVVLDEEAGRIEVVVPDDQLSLAIGRRGQNVRLASQLTQWNIDILTEAEESERRTEEFRS
HHHHCCCCCEEEEEECCCCEEHHCCCCCCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHH
RSNMFIQALDVDDVIAHLLVTEGFSSVEEVAYVPAEDIADIEGFDENVAEELQTRARNFL
HCCCEEEEECHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHH
AEQDERYDQMRKDMGVADEMAAIEGLAPSMLVKLGDKGIKTLDDLGDLAGDELIDILGKD
HHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
AMTEDEANAVIMAARAHWFEEGK
CCCCCCCCEEEEEHHHHHHCCCC
>Mature Secondary Structure
MERIAALPRPELLQVADAVARDKGIDRDEVLEAMEQAIQKAGRSKYGHEHDIRAHIDRKT
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCC
GEIQLARYIEVVEEVENEATQQTLKQVLKKKPDAVVGDFLVDPLPPIDFGRIAAQTAKQV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHH
IVQKVRDAERQRQFNEYKDRVGEISNGLVKRVEFGNVIVDLGRAEALLRRDELIPRETFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECHHHHHHHHHHHCCCHHHHC
TGDRVRAYIYDVRQEPRGPQIFLSRTHPIFMAKLFAQEVPEIYDGIIEIKAVARDPGSRA
CCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KIAVLSHDSSIDPVGACVGMRGSRVQAVVAELQGEKIDIIQWSPDVATFVVNGLAPAEVT
EEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCHHHHH
KVVLDEEAGRIEVVVPDDQLSLAIGRRGQNVRLASQLTQWNIDILTEAEESERRTEEFRS
HHHHCCCCCEEEEEECCCCEEHHCCCCCCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHH
RSNMFIQALDVDDVIAHLLVTEGFSSVEEVAYVPAEDIADIEGFDENVAEELQTRARNFL
HCCCEEEEECHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHH
AEQDERYDQMRKDMGVADEMAAIEGLAPSMLVKLGDKGIKTLDDLGDLAGDELIDILGKD
HHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
AMTEDEANAVIMAARAHWFEEGK
CCCCCCCCEEEEEHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]