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The map label for this gene is trpF

Identifier: 83313100

GI number: 83313100

Start: 4410968

End: 4411615

Strand: Reverse

Name: trpF

Synonym: amb4001

Alternate gene names: 83313100

Gene position: 4411615-4410968 (Counterclockwise)

Preceding gene: 83313101

Following gene: 83313096

Centisome position: 88.82

GC content: 69.44

Gene sequence:

>648_bases
ATGCCGGTCGAGGTCAAGATCTGCGGCATCACCGATGAAGACGCTATGGACGTGGCCATCGAGGAGGGCGCCGATTACGT
CGGTCTGGTGTTCTTTCCCCCCAGCCCCCGCAACGTCACCCCGGACCGTGCCGCCGAACTGGTGGAGTTCGCGCCCGGTG
ACGTGACCAAGGTCGGCCTGTTCGTCGATCCCGACGATGCCACCCTGGACACGGTGCTGACCCGGGTGCGGCTCGACCTG
CTGCAACTGCATGGGCACGAGACCCCCGAGCGGGTCGAGGCCATCCGCCTGGAATACGGCCTGCCCGTCATGAAGGTGCT
GTCGGTGTCGGACGCCGCCGATCTTGACGCCGCCGAGCCCTATCTGGCGGTGGCCGACCGCCTGCTGTTCGACGCCAAGC
CGCCTAAGGGCGCGGTCCTGCCCGGCGGCAACGCGGTCAGCTTCGACTGGTCCATCCTGACGGGGCGCAAATGGGGCCTG
CCCTGGATGCTGGCCGGCGGCCTGACCCCCGCCAACGTGGCCGAGGCGGTCCGCATCAGCGGTGCCGCCGCCGTCGACGT
CTCGTCGGGGGTGGAAAGCGCCCCCGGCATCAAGGATTCCGACAAGATCCGCGCCTTCATCAAGGCGGCCCGGGGCGGCC
GCCCGTAG

Upstream 100 bases:

>100_bases
AAGGGCGCCGACGTGCTGGTGATCGGGCGCCCCATCACCGCCGCCGCCGATCCGGCCGAGGCAGCCGGGCGGATCAAGGC
TGAACTGGGGCTGTAGCACC

Downstream 100 bases:

>100_bases
GGGCTGCCTTAGGATTTATTGCTTCGCAATTGGGCTTTGCCCAAACCCACCGGGGAGAACCCCGGCCCCCTTTTTTCTTA
TAAATTCAGGGGAGGTTTGG

Product: N-(5'-phosphoribosyl)anthranilate isomerase

Products: NA

Alternate protein names: PRAI

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MPVEVKICGITDEDAMDVAIEEGADYVGLVFFPPSPRNVTPDRAAELVEFAPGDVTKVGLFVDPDDATLDTVLTRVRLDL
LQLHGHETPERVEAIRLEYGLPVMKVLSVSDAADLDAAEPYLAVADRLLFDAKPPKGAVLPGGNAVSFDWSILTGRKWGL
PWMLAGGLTPANVAEAVRISGAAAVDVSSGVESAPGIKDSDKIRAFIKAARGGRP

Sequences:

>Translated_215_residues
MPVEVKICGITDEDAMDVAIEEGADYVGLVFFPPSPRNVTPDRAAELVEFAPGDVTKVGLFVDPDDATLDTVLTRVRLDL
LQLHGHETPERVEAIRLEYGLPVMKVLSVSDAADLDAAEPYLAVADRLLFDAKPPKGAVLPGGNAVSFDWSILTGRKWGL
PWMLAGGLTPANVAEAVRISGAAAVDVSSGVESAPGIKDSDKIRAFIKAARGGRP
>Mature_214_residues
PVEVKICGITDEDAMDVAIEEGADYVGLVFFPPSPRNVTPDRAAELVEFAPGDVTKVGLFVDPDDATLDTVLTRVRLDLL
QLHGHETPERVEAIRLEYGLPVMKVLSVSDAADLDAAEPYLAVADRLLFDAKPPKGAVLPGGNAVSFDWSILTGRKWGLP
WMLAGGLTPANVAEAVRISGAAAVDVSSGVESAPGIKDSDKIRAFIKAARGGRP

Specific function: Bifunctional Enzyme That Catalyzes Two Sequential Steps Of Tryptophan Biosynthetic Pathway. The First Reaction Is Catalyzed By The Isomerase, Coded By The Trpf Domain; The Second Reaction Is Catalyzed By The Synthase, Coded By The Trpc Domain. [C]

COG id: COG0135

COG function: function code E; Phosphoribosylanthranilate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpF family

Homologues:

Organism=Escherichia coli, GI87081863, Length=210, Percent_Identity=34.2857142857143, Blast_Score=94, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6320210, Length=215, Percent_Identity=32.093023255814, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRPF_MAGSA (Q2W020)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_423364.1
- ProteinModelPortal:   Q2W020
- SMR:   Q2W020
- STRING:   Q2W020
- GeneID:   3804918
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb4001
- NMPDR:   fig|342108.5.peg.3594
- eggNOG:   COG0135
- HOGENOM:   HBG554803
- OMA:   SKICGIT
- PhylomeDB:   Q2W020
- ProtClustDB:   PRK01222
- BioCyc:   MMAG342108:AMB4001-MONOMER
- HAMAP:   MF_00135_B
- InterPro:   IPR013785
- InterPro:   IPR001240
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00697 PRAI; SSF51366 RibP_bind_barrel

EC number: =5.3.1.24

Molecular weight: Translated: 22757; Mature: 22626

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVEVKICGITDEDAMDVAIEEGADYVGLVFFPPSPRNVTPDRAAELVEFAPGDVTKVGL
CCEEEEEECCCCCCHHEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEE
FVDPDDATLDTVLTRVRLDLLQLHGHETPERVEAIRLEYGLPVMKVLSVSDAADLDAAEP
EECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCH
YLAVADRLLFDAKPPKGAVLPGGNAVSFDWSILTGRKWGLPWMLAGGLTPANVAEAVRIS
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHC
GAAAVDVSSGVESAPGIKDSDKIRAFIKAARGGRP
CCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PVEVKICGITDEDAMDVAIEEGADYVGLVFFPPSPRNVTPDRAAELVEFAPGDVTKVGL
CEEEEEECCCCCCHHEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEE
FVDPDDATLDTVLTRVRLDLLQLHGHETPERVEAIRLEYGLPVMKVLSVSDAADLDAAEP
EECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCH
YLAVADRLLFDAKPPKGAVLPGGNAVSFDWSILTGRKWGLPWMLAGGLTPANVAEAVRIS
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHC
GAAAVDVSSGVESAPGIKDSDKIRAFIKAARGGRP
CCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA