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The map label for this gene is pyrF

Identifier: 83313101

GI number: 83313101

Start: 4411620

End: 4412327

Strand: Reverse

Name: pyrF

Synonym: amb4002

Alternate gene names: 83313101

Gene position: 4412327-4411620 (Counterclockwise)

Preceding gene: 83313102

Following gene: 83313100

Centisome position: 88.83

GC content: 70.76

Gene sequence:

>708_bases
GTGATCAATCCCGTCTATGTCGCCATCGACACCACCGAAGCCGCCCGCGCCATCGCCCTTGCCGAACGCCTGAAGGGGCA
GGTCGGCGGCTTCAAGCTGGGCCTGGAATACTTCACCGCCAACGGCCCCGCCGGCATGGAGGCGGTGTCCGGCCTGGGCA
TGCCGCTGTTCGTCGATCTCAAGCTGCACGATATTCCCAATACCGTGGCCGCCGCCATGAAGGGCGTGGTGCGCCTGGGC
GCCGCCATCACCACCATTCACGCCTCGGGCGGCGCCGCCATGATCCGCGCCGCCGTGGACGCGGCCAATGACGAGGCGGC
CAAACTCGGCATCGCTCCGCCCGCCGTGGTGGCGGTGACCGTGCTGACCAGCCTGGACCAGGCCGGGGCCGAGCAGGTGG
GCTTTGAGCGTCCGGTGCTGGATCAGGTCAAGCGTCTGGCCACCCTGGCCCAGGACAGCGGCGCCGCCGGCATCGTCTGC
TCGCCCCTCGAGGTGGAGGCGGTGCGCGCCCTGTGTGGTCCCGATTTCAAGCTGGTCATTCCCGGCATCCGCCCCGCCTG
GAGCGAGGCCGGCGACCAGAAGCGCTTCCTTACCCCCGCCGAGGCCCGCGCCAAGGGCGCCGACGTGCTGGTGATCGGGC
GCCCCATCACCGCCGCCGCCGATCCGGCCGAGGCAGCCGGGCGGATCAAGGCTGAACTGGGGCTGTAG

Upstream 100 bases:

>100_bases
CTGGACCTCGGGGCTGAAGGCCCGCCTGCGCGCCGGGTCGCTGCAGCGCAAGCTGGCCGCCGCCGAAAAGACCCAGTCTC
AAGTCTGAGGTAGATACCCC

Downstream 100 bases:

>100_bases
CACCATGCCGGTCGAGGTCAAGATCTGCGGCATCACCGATGAAGACGCTATGGACGTGGCCATCGAGGAGGGCGCCGATT
ACGTCGGTCTGGTGTTCTTT

Product: orotidine-5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MINPVYVAIDTTEAARAIALAERLKGQVGGFKLGLEYFTANGPAGMEAVSGLGMPLFVDLKLHDIPNTVAAAMKGVVRLG
AAITTIHASGGAAMIRAAVDAANDEAAKLGIAPPAVVAVTVLTSLDQAGAEQVGFERPVLDQVKRLATLAQDSGAAGIVC
SPLEVEAVRALCGPDFKLVIPGIRPAWSEAGDQKRFLTPAEARAKGADVLVIGRPITAAADPAEAAGRIKAELGL

Sequences:

>Translated_235_residues
MINPVYVAIDTTEAARAIALAERLKGQVGGFKLGLEYFTANGPAGMEAVSGLGMPLFVDLKLHDIPNTVAAAMKGVVRLG
AAITTIHASGGAAMIRAAVDAANDEAAKLGIAPPAVVAVTVLTSLDQAGAEQVGFERPVLDQVKRLATLAQDSGAAGIVC
SPLEVEAVRALCGPDFKLVIPGIRPAWSEAGDQKRFLTPAEARAKGADVLVIGRPITAAADPAEAAGRIKAELGL
>Mature_235_residues
MINPVYVAIDTTEAARAIALAERLKGQVGGFKLGLEYFTANGPAGMEAVSGLGMPLFVDLKLHDIPNTVAAAMKGVVRLG
AAITTIHASGGAAMIRAAVDAANDEAAKLGIAPPAVVAVTVLTSLDQAGAEQVGFERPVLDQVKRLATLAQDSGAAGIVC
SPLEVEAVRALCGPDFKLVIPGIRPAWSEAGDQKRFLTPAEARAKGADVLVIGRPITAAADPAEAAGRIKAELGL

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=231, Percent_Identity=41.1255411255411, Blast_Score=167, Evalue=6e-43,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_MAGSA (Q2W019)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_423365.1
- STRING:   Q2W019
- GeneID:   3804919
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb4002
- NMPDR:   fig|342108.5.peg.3595
- eggNOG:   COG0284
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- PhylomeDB:   Q2W019
- BioCyc:   MMAG342108:AMB4002-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 23950; Mature: 23950

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 61-61 BINDING 10-10 BINDING 32-32 BINDING 123-123 BINDING 184-184 BINDING 193-193 BINDING 213-213 BINDING 214-214

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINPVYVAIDTTEAARAIALAERLKGQVGGFKLGLEYFTANGPAGMEAVSGLGMPLFVDL
CCCCEEEEEECHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHCCCCCEEEEE
KLHDIPNTVAAAMKGVVRLGAAITTIHASGGAAMIRAAVDAANDEAAKLGIAPPAVVAVT
EEECCCHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHCCCCCHHEECCCCHHHHHHH
VLTSLDQAGAEQVGFERPVLDQVKRLATLAQDSGAAGIVCSPLEVEAVRALCGPDFKLVI
HHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHCCCCEEEE
PGIRPAWSEAGDQKRFLTPAEARAKGADVLVIGRPITAAADPAEAAGRIKAELGL
CCCCCCHHCCCCCCCCCCCHHHHCCCCCEEEECCCCCCCCCCHHHHCCEEEECCC
>Mature Secondary Structure
MINPVYVAIDTTEAARAIALAERLKGQVGGFKLGLEYFTANGPAGMEAVSGLGMPLFVDL
CCCCEEEEEECHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHCCCCCEEEEE
KLHDIPNTVAAAMKGVVRLGAAITTIHASGGAAMIRAAVDAANDEAAKLGIAPPAVVAVT
EEECCCHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHCCCCCHHEECCCCHHHHHHH
VLTSLDQAGAEQVGFERPVLDQVKRLATLAQDSGAAGIVCSPLEVEAVRALCGPDFKLVI
HHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHCCCCEEEE
PGIRPAWSEAGDQKRFLTPAEARAKGADVLVIGRPITAAADPAEAAGRIKAELGL
CCCCCCHHCCCCCCCCCCCHHHHCCCCCEEEECCCCCCCCCCHHHHCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA