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The map label for this gene is aroK

Identifier: 83313025

GI number: 83313025

Start: 4338190

End: 4338777

Strand: Reverse

Name: aroK

Synonym: amb3926

Alternate gene names: 83313025

Gene position: 4338777-4338190 (Counterclockwise)

Preceding gene: 83313030

Following gene: 83313024

Centisome position: 87.35

GC content: 67.86

Gene sequence:

>588_bases
TTGGATCAGGGCATGGCCGACGAACTGGCGAGACTGGCGCCGCTGCTGTCAGGGCGGATCGGCCGCACGATCGTCCTGGT
CGGCCTGATGGGCGCGGGCAAGTCCTGCGTCGGCCGCCGCCTCGCCGCCCGTCTGGGGCTGGACTTCGTCGATTCCGACG
CCGAGTTCGAGGCCGCCTCGGGCAGCAGCATCAGCGACTACTTCGCCCGCTTCGGCGAGGCCGCTTTCCGCGAGGGCGAG
CGCAAGGTCATCGCCCGCCTGCTGGACGGCCCGCCGGTGGTCCTGGCCACGGGCGGCGGAGCCTTTGTCGACCCCACCAC
CCGCGAGCGCATCAAGGCCGCCGGGACCTCGGTGTGGATCCGCGCCGATCTCGAGCTTTTGCTGAAGCGGACCGTGGGCC
GCGACCACCGTCCGCTGCTAAAGCAGGGCGACCCCCGTGAAATTCTCGGCCGGTTGATGGAAGCCCGCTATCCCATCTAT
GCCGAAGCCGACATTATCGTCGAGTCCACCGACGAAGTCCCCGAAGCCACCGTGATCCGCGTCATGGAGGGGCTGATCAC
CTATCTGGATCTGGAGAAGACCGTATGA

Upstream 100 bases:

>100_bases
ACGGCCCCGTATGCGTACGTGCGGGCAGTGTATCGGCCAAGGCGACGGTGATCAACGCCGGCTCGCCGGTTTTTTTAAAC
TTCAAGGAGCGGGACCAACC

Downstream 100 bases:

>100_bases
GTGCCGCCGCCGAAGAGGTCATGGTCGAACTGGGCGAGCGCTCGTATCCGATCCATATCGGCCCCGGCCTGCTGGACCGG
GCGGGCGAGCTGATCAAGCC

Product: shikimate kinase

Products: NA

Alternate protein names: SK

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MDQGMADELARLAPLLSGRIGRTIVLVGLMGAGKSCVGRRLAARLGLDFVDSDAEFEAASGSSISDYFARFGEAAFREGE
RKVIARLLDGPPVVLATGGGAFVDPTTRERIKAAGTSVWIRADLELLLKRTVGRDHRPLLKQGDPREILGRLMEARYPIY
AEADIIVESTDEVPEATVIRVMEGLITYLDLEKTV

Sequences:

>Translated_195_residues
MDQGMADELARLAPLLSGRIGRTIVLVGLMGAGKSCVGRRLAARLGLDFVDSDAEFEAASGSSISDYFARFGEAAFREGE
RKVIARLLDGPPVVLATGGGAFVDPTTRERIKAAGTSVWIRADLELLLKRTVGRDHRPLLKQGDPREILGRLMEARYPIY
AEADIIVESTDEVPEATVIRVMEGLITYLDLEKTV
>Mature_195_residues
MDQGMADELARLAPLLSGRIGRTIVLVGLMGAGKSCVGRRLAARLGLDFVDSDAEFEAASGSSISDYFARFGEAAFREGE
RKVIARLLDGPPVVLATGGGAFVDPTTRERIKAAGTSVWIRADLELLLKRTVGRDHRPLLKQGDPREILGRLMEARYPIY
AEADIIVESTDEVPEATVIRVMEGLITYLDLEKTV

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family

Homologues:

Organism=Escherichia coli, GI87082255, Length=166, Percent_Identity=40.3614457831325, Blast_Score=116, Evalue=9e-28,
Organism=Escherichia coli, GI1786587, Length=153, Percent_Identity=30.0653594771242, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): AROK_MAGSA (Q2W095)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_423289.1
- ProteinModelPortal:   Q2W095
- SMR:   Q2W095
- STRING:   Q2W095
- GeneID:   3805547
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb3926
- NMPDR:   fig|342108.5.peg.3521
- eggNOG:   COG0703
- HOGENOM:   HBG335815
- OMA:   VIYLRAN
- PhylomeDB:   Q2W095
- BioCyc:   MMAG342108:AMB3926-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00109
- InterPro:   IPR000623
- InterPro:   IPR023000
- PRINTS:   PR01100

Pfam domain/function: PF01202 SKI

EC number: =2.7.1.71

Molecular weight: Translated: 21187; Mature: 21187

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: BINDING 53-53 BINDING 77-77 BINDING 99-99 BINDING 137-137 BINDING 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQGMADELARLAPLLSGRIGRTIVLVGLMGAGKSCVGRRLAARLGLDFVDSDAEFEAAS
CCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
GSSISDYFARFGEAAFREGERKVIARLLDGPPVVLATGGGAFVDPTTRERIKAAGTSVWI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCEEEE
RADLELLLKRTVGRDHRPLLKQGDPREILGRLMEARYPIYAEADIIVESTDEVPEATVIR
HHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHCCCCEECCEEEEECCCCCCHHHHHH
VMEGLITYLDLEKTV
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MDQGMADELARLAPLLSGRIGRTIVLVGLMGAGKSCVGRRLAARLGLDFVDSDAEFEAAS
CCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
GSSISDYFARFGEAAFREGERKVIARLLDGPPVVLATGGGAFVDPTTRERIKAAGTSVWI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCEEEE
RADLELLLKRTVGRDHRPLLKQGDPREILGRLMEARYPIYAEADIIVESTDEVPEATVIR
HHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHCCCCEECCEEEEECCCCCCHHHHHH
VMEGLITYLDLEKTV
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA