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The map label for this gene is gacA [H]

Identifier: 83312959

GI number: 83312959

Start: 4263981

End: 4264658

Strand: Reverse

Name: gacA [H]

Synonym: amb3860

Alternate gene names: 83312959

Gene position: 4264658-4263981 (Counterclockwise)

Preceding gene: 83312969

Following gene: 83312958

Centisome position: 85.86

GC content: 65.93

Gene sequence:

>678_bases
GTGATCACGAGTGTGGTGGCGGTGATGAGTGGTGATCGGGGGGCGGTTCGGACGGTACTTGTGGATGATCACGGGATTGT
GCGGACGGCGATCCGCCGCGCCTTGGAAGGCATGGCGTGGATCGACGTGGTCGGCGAGGCGGATGACGGCGTGGCCGCGC
TGCATCTGATCGACGACGCCAAGCCCGATTTGATCATTCTGGACCTTTCCATGCCGGGGCTTGACGGGATGGACATCATT
CCCCTGGTCAAGCGGCGCAATCCCGCCAGCCGGATCGTGGTTCTGACCGGGACCCAGGACGGAACGACCATGAAGCGGGC
CTTTGCCCTGGGTGTGGACGCCTACACCTTGAAGACGGGCGGCTGGCAGGACCTGGAAACCGCCATCGGCGAGGCCATGG
CCGGGCGGCGCTATATCTCGCCATCGGCCGGGCTGGTGGTGGATGGCGGCGAAGAGGGAGGCCTGGCGGCTGGCAGCGCC
GTGGCGCCGCTGTCGGGGCGCGAGCGTCAGGTGCTGAAACTGCTGGCGGAGGGCTACACCAACCAGAAGGTGGCGGGAAT
CCTGGGAATCAGTGTCAAGACGGTGGACCGGCACCGCGAGAACATCATGAACAAATTGGGGGTGAGGACACCGGTGGCCT
TGGCGGCCATCGCCCTGCGGGAGGGGCTGGTGGTCTGA

Upstream 100 bases:

>100_bases
GCTTACGGCATACCTTTTGTGTTCGATATATATACAGTATGTCATGCCGAGATCTCGAAAAAAGCCTTGAGCGGCCGTGA
TATCCTCTCCAAGGGACTGG

Downstream 100 bases:

>100_bases
CCCCGCAACACGGCGACAGGGGTGGGATGCGACATGAGTGACGACCAGGATCTGCTGAAGGCTATCGCCCAGATACGCCG
CTCGCCCTATGGCAAGCGGG

Product: response regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MITSVVAVMSGDRGAVRTVLVDDHGIVRTAIRRALEGMAWIDVVGEADDGVAALHLIDDAKPDLIILDLSMPGLDGMDII
PLVKRRNPASRIVVLTGTQDGTTMKRAFALGVDAYTLKTGGWQDLETAIGEAMAGRRYISPSAGLVVDGGEEGGLAAGSA
VAPLSGRERQVLKLLAEGYTNQKVAGILGISVKTVDRHRENIMNKLGVRTPVALAAIALREGLVV

Sequences:

>Translated_225_residues
MITSVVAVMSGDRGAVRTVLVDDHGIVRTAIRRALEGMAWIDVVGEADDGVAALHLIDDAKPDLIILDLSMPGLDGMDII
PLVKRRNPASRIVVLTGTQDGTTMKRAFALGVDAYTLKTGGWQDLETAIGEAMAGRRYISPSAGLVVDGGEEGGLAAGSA
VAPLSGRERQVLKLLAEGYTNQKVAGILGISVKTVDRHRENIMNKLGVRTPVALAAIALREGLVV
>Mature_225_residues
MITSVVAVMSGDRGAVRTVLVDDHGIVRTAIRRALEGMAWIDVVGEADDGVAALHLIDDAKPDLIILDLSMPGLDGMDII
PLVKRRNPASRIVVLTGTQDGTTMKRAFALGVDAYTLKTGGWQDLETAIGEAMAGRRYISPSAGLVVDGGEEGGLAAGSA
VAPLSGRERQVLKLLAEGYTNQKVAGILGISVKTVDRHRENIMNKLGVRTPVALAAIALREGLVV

Specific function: Positively controls the production of the autoinducer N- butyryl-homoserine lactone and the formation of the virulence factors pyocyanine, cyanide, and lipase [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788546, Length=202, Percent_Identity=32.1782178217822, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788222, Length=209, Percent_Identity=30.1435406698565, Blast_Score=101, Evalue=3e-23,
Organism=Escherichia coli, GI1788521, Length=206, Percent_Identity=32.0388349514563, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1786747, Length=196, Percent_Identity=27.5510204081633, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788712, Length=209, Percent_Identity=25.3588516746411, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787473, Length=181, Percent_Identity=33.7016574585635, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1790102, Length=201, Percent_Identity=29.3532338308458, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23628; Mature: 23628

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITSVVAVMSGDRGAVRTVLVDDHGIVRTAIRRALEGMAWIDVVGEADDGVAALHLIDDA
CCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCC
KPDLIILDLSMPGLDGMDIIPLVKRRNPASRIVVLTGTQDGTTMKRAFALGVDAYTLKTG
CCCEEEEEECCCCCCCCCHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHCCCCEEEECCC
GWQDLETAIGEAMAGRRYISPSAGLVVDGGEEGGLAAGSAVAPLSGRERQVLKLLAEGYT
CCHHHHHHHHHHHCCCEEECCCCCEEEECCCCCCCCCCCEECCCCCCHHHHHHHHHCCCC
NQKVAGILGISVKTVDRHRENIMNKLGVRTPVALAAIALREGLVV
CCEEEEEEEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MITSVVAVMSGDRGAVRTVLVDDHGIVRTAIRRALEGMAWIDVVGEADDGVAALHLIDDA
CCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCC
KPDLIILDLSMPGLDGMDIIPLVKRRNPASRIVVLTGTQDGTTMKRAFALGVDAYTLKTG
CCCEEEEEECCCCCCCCCHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHCCCCEEEECCC
GWQDLETAIGEAMAGRRYISPSAGLVVDGGEEGGLAAGSAVAPLSGRERQVLKLLAEGYT
CCHHHHHHHHHHHCCCEEECCCCCEEEECCCCCCCCCCCEECCCCCCHHHHHHHHHCCCC
NQKVAGILGISVKTVDRHRENIMNKLGVRTPVALAAIALREGLVV
CCEEEEEEEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9159518; 10984043 [H]