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The map label for this gene is pleD [H]

Identifier: 83312731

GI number: 83312731

Start: 3991794

End: 3993671

Strand: Reverse

Name: pleD [H]

Synonym: amb3632

Alternate gene names: 83312731

Gene position: 3993671-3991794 (Counterclockwise)

Preceding gene: 83312733

Following gene: 83312729

Centisome position: 80.4

GC content: 69.12

Gene sequence:

>1878_bases
ATGACTATCGTCGCCGTCATGGAGGGGGCCTGCCCCCGGTTGGAGGAGCAGATCCGCCTGCTGGGATGGCCTCGGGTGGC
GGCCCCGGGAGAGGGCGGCAGAAACGACGAGCGCGCGGTCATGGTGCGCGAGGCCTTCACGCCCCAGGCCCTGATCGATT
GCCTTTCTCCGCCCTGGATCGGGGGAATCGAGGCCGCCGGCTCGGGCGGGCATCCGGTGGGGTCGTGCTTCCGCCGCCAT
CTGGCGGAAGGAGGGCTGGGCCTGTCGCTGCGCTCGGATACCGCCTATGGCGCCGACATTGCCGTCCCGTTCGCCGATGC
CCTGGCGACTCGGCTGCTCGCCCCCGGAACCGACAGTTTCGATCTGGGATTCGTCGTCCAGGAGCTGGTCTCCAATGCCC
TGTTGCACGGCAATCTGGAAGTGGGCGGCATCGACTGCGAGGGCGGCGGTGGGCTCGAAGGCTTCGGCGAGCGGATCGAG
GCGGCTCTTGGCGATCCCGGCCGGGCCGGGCGGCGGGTGCAGATTTCCGCCTCGGTGGTTGGCGATCGTCTGGAGGTCGC
CGTCGAGGATGACGGCCCCGGCTTCGATCACGTGGACGGCCATGCCAATCCCCGACGGCCGCACGGGCTGGCCCTGCTGG
AAAGCATGGTTTCGGACCTGCACACCGAAGAAGGGGGGCGCCGGGCCATCGCCACCATGGTCATTCCCACCCGCCAGTTG
CGGGCCGAGCCGCTGGGCCTGGACGATGTGCGGGTGCTGGTGGTGGACGACAACCTGATGAACCGGGTGCTGATGGAAAC
CCTGCTGCGCCGCATGGGAGTCGGCCAGGTGGAGACCGTGGAATCCGGCGAAAAGGGCCTGGCCGCCATCGAGCGGGAAA
AGCCCGATCTGGTGCTGCTCGACGTCATGATGCCCGGCATGGACGGGTTCGAGATGTGCCGGCGCCTGCGCCGCCTTCAC
CCCCTGACCGAGCTGCCGGTGATCTTCGTCACCGCGCTTGACGGCCCCGCCGACCGCAATGCCTGCTTCGCCGTGGGCGG
CAGCGACATGGTGGCCAAGCCCATCGACGCCAACGAGGTTGCCGCCCGGGTGGGCGTGCATCTGCGCCTGGGCCAGGCCA
TGGATCGGCTGACCGCGTATCAGGACAGGGTTCACGAGGAGTTGCGCGCCGCCCGCGGCGCCCAGGCTGCCCTGATGCCC
ACCTCGGCCGAATTGTCGTCCATTCGCATCCGTCAGGGGCTGGTGGTGGATGGGCGAATCGAAAGCTCTTCCGAACTGGG
GGGGGATTTCTGGACGGTGCTGCCCGCCGGTCCCAACCAGATGTTCCTGCTGGTGGCCGACTTCACCGGTCACGGCCCGG
TGGCGGCGTTCAACGTCTTTCGCCTGCACCTTCTGCTGTCGCGCCTGCCGCGGCGGATGCCCACGCCATCGGCCCTGTTG
GAATATCTCAATCTGGAACTGAAGGCGGTTCTGCGCTCCGGCCAGTTCGCCGCCGCCTTCGCCGCGCTGATCGACGTGGA
AGAAGGGGTCATGACCTATGCCGGCGCGGCCTCGCCGCCGGCGGTGCTGGTGGTGGACGGCGAGGTCCGCTTCCTCGATG
CCGACGGGCCGCCGCTCGGCGCCTTCGTCGATGCCGAATACGAGGAAAGCCGCATCGCTTTGCCGCGTGGAGCCGCCTTG
CTGGCCTATTCCGACGCCCTGGTGGAAAGCCAGGGAAGCGACGGCCGCATGGCCTGCGACCAGGAGACGCTGCGGGAGTG
GGTGCTGGGCGCCCAGCCCGGCCATTCCCTGGCCGACGAGGTGCTGGCCCGGTTCCGGGAAAAGGTACCGGGCGAGCCGC
CGGACGACCTGACCCTGGTCAGCATCCGGCGGCCCTGA

Upstream 100 bases:

>100_bases
AATCGAAATCGTACTCCATAATGCCCCCGTCGTTTTCATGGACCAAGCAGACCTTCCCATAACCTAGATTTGCCTTAAAT
TTGCGCCGGAGCAAGGAACA

Downstream 100 bases:

>100_bases
CCGGCGACGATCAGCTGAAGGCGGTGGCCAACTCGTCCAGCAGGGTCTGAACCTCCTGGGACGGGCCTTCGATGGCCGCC
ACCGAATCCACCGCGCCGTC

Product: chemotaxis response regulator protein-glutamate methylesterase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 625; Mature: 624

Protein sequence:

>625_residues
MTIVAVMEGACPRLEEQIRLLGWPRVAAPGEGGRNDERAVMVREAFTPQALIDCLSPPWIGGIEAAGSGGHPVGSCFRRH
LAEGGLGLSLRSDTAYGADIAVPFADALATRLLAPGTDSFDLGFVVQELVSNALLHGNLEVGGIDCEGGGGLEGFGERIE
AALGDPGRAGRRVQISASVVGDRLEVAVEDDGPGFDHVDGHANPRRPHGLALLESMVSDLHTEEGGRRAIATMVIPTRQL
RAEPLGLDDVRVLVVDDNLMNRVLMETLLRRMGVGQVETVESGEKGLAAIEREKPDLVLLDVMMPGMDGFEMCRRLRRLH
PLTELPVIFVTALDGPADRNACFAVGGSDMVAKPIDANEVAARVGVHLRLGQAMDRLTAYQDRVHEELRAARGAQAALMP
TSAELSSIRIRQGLVVDGRIESSSELGGDFWTVLPAGPNQMFLLVADFTGHGPVAAFNVFRLHLLLSRLPRRMPTPSALL
EYLNLELKAVLRSGQFAAAFAALIDVEEGVMTYAGAASPPAVLVVDGEVRFLDADGPPLGAFVDAEYEESRIALPRGAAL
LAYSDALVESQGSDGRMACDQETLREWVLGAQPGHSLADEVLARFREKVPGEPPDDLTLVSIRRP

Sequences:

>Translated_625_residues
MTIVAVMEGACPRLEEQIRLLGWPRVAAPGEGGRNDERAVMVREAFTPQALIDCLSPPWIGGIEAAGSGGHPVGSCFRRH
LAEGGLGLSLRSDTAYGADIAVPFADALATRLLAPGTDSFDLGFVVQELVSNALLHGNLEVGGIDCEGGGGLEGFGERIE
AALGDPGRAGRRVQISASVVGDRLEVAVEDDGPGFDHVDGHANPRRPHGLALLESMVSDLHTEEGGRRAIATMVIPTRQL
RAEPLGLDDVRVLVVDDNLMNRVLMETLLRRMGVGQVETVESGEKGLAAIEREKPDLVLLDVMMPGMDGFEMCRRLRRLH
PLTELPVIFVTALDGPADRNACFAVGGSDMVAKPIDANEVAARVGVHLRLGQAMDRLTAYQDRVHEELRAARGAQAALMP
TSAELSSIRIRQGLVVDGRIESSSELGGDFWTVLPAGPNQMFLLVADFTGHGPVAAFNVFRLHLLLSRLPRRMPTPSALL
EYLNLELKAVLRSGQFAAAFAALIDVEEGVMTYAGAASPPAVLVVDGEVRFLDADGPPLGAFVDAEYEESRIALPRGAAL
LAYSDALVESQGSDGRMACDQETLREWVLGAQPGHSLADEVLARFREKVPGEPPDDLTLVSIRRP
>Mature_624_residues
TIVAVMEGACPRLEEQIRLLGWPRVAAPGEGGRNDERAVMVREAFTPQALIDCLSPPWIGGIEAAGSGGHPVGSCFRRHL
AEGGLGLSLRSDTAYGADIAVPFADALATRLLAPGTDSFDLGFVVQELVSNALLHGNLEVGGIDCEGGGGLEGFGERIEA
ALGDPGRAGRRVQISASVVGDRLEVAVEDDGPGFDHVDGHANPRRPHGLALLESMVSDLHTEEGGRRAIATMVIPTRQLR
AEPLGLDDVRVLVVDDNLMNRVLMETLLRRMGVGQVETVESGEKGLAAIEREKPDLVLLDVMMPGMDGFEMCRRLRRLHP
LTELPVIFVTALDGPADRNACFAVGGSDMVAKPIDANEVAARVGVHLRLGQAMDRLTAYQDRVHEELRAARGAQAALMPT
SAELSSIRIRQGLVVDGRIESSSELGGDFWTVLPAGPNQMFLLVADFTGHGPVAAFNVFRLHLLLSRLPRRMPTPSALLE
YLNLELKAVLRSGQFAAAFAALIDVEEGVMTYAGAASPPAVLVVDGEVRFLDADGPPLGAFVDAEYEESRIALPRGAALL
AYSDALVESQGSDGRMACDQETLREWVLGAQPGHSLADEVLARFREKVPGEPPDDLTLVSIRRP

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG0784

COG function: function code T; FOG: CheY-like receiver

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1789809, Length=122, Percent_Identity=36.8852459016393, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1790860, Length=158, Percent_Identity=34.1772151898734, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788394, Length=127, Percent_Identity=33.0708661417323, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI145693157, Length=120, Percent_Identity=35, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790299, Length=113, Percent_Identity=35.3982300884956, Blast_Score=66, Evalue=8e-12,
Organism=Escherichia coli, GI87082012, Length=126, Percent_Identity=30.952380952381, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1789149, Length=108, Percent_Identity=38.8888888888889, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 66801; Mature: 66670

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIVAVMEGACPRLEEQIRLLGWPRVAAPGEGGRNDERAVMVREAFTPQALIDCLSPPWI
CEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEHHCCHHHHHHHCCCCCC
GGIEAAGSGGHPVGSCFRRHLAEGGLGLSLRSDTAYGADIAVPFADALATRLLAPGTDSF
CCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEECHHHHHHHHHHHCCCCCCC
DLGFVVQELVSNALLHGNLEVGGIDCEGGGGLEGFGERIEAALGDPGRAGRRVQISASVV
HHHHHHHHHHHHHHEECCCEEECEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEHEEC
GDRLEVAVEDDGPGFDHVDGHANPRRPHGLALLESMVSDLHTEEGGRRAIATMVIPTRQL
CCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECHHHH
RAEPLGLDDVRVLVVDDNLMNRVLMETLLRRMGVGQVETVESGEKGLAAIEREKPDLVLL
CCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHCCCCCEEEE
DVMMPGMDGFEMCRRLRRLHPLTELPVIFVTALDGPADRNACFAVGGSDMVAKPIDANEV
EECCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEECCCCCEECCCCHHHH
AARVGVHLRLGQAMDRLTAYQDRVHEELRAARGAQAALMPTSAELSSIRIRQGLVVDGRI
HHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHEEHHCCEEEECCC
ESSSELGGDFWTVLPAGPNQMFLLVADFTGHGPVAAFNVFRLHLLLSRLPRRMPTPSALL
CCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
EYLNLELKAVLRSGQFAAAFAALIDVEEGVMTYAGAASPPAVLVVDGEVRFLDADGPPLG
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHCCCCCCCEEEEECCEEEEEECCCCCCC
AFVDAEYEESRIALPRGAALLAYSDALVESQGSDGRMACDQETLREWVLGAQPGHSLADE
EEEECCCCCCEEECCCCCEEEEEHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHH
VLARFREKVPGEPPDDLTLVSIRRP
HHHHHHHHCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
TIVAVMEGACPRLEEQIRLLGWPRVAAPGEGGRNDERAVMVREAFTPQALIDCLSPPWI
EEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEHHCCHHHHHHHCCCCCC
GGIEAAGSGGHPVGSCFRRHLAEGGLGLSLRSDTAYGADIAVPFADALATRLLAPGTDSF
CCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEECHHHHHHHHHHHCCCCCCC
DLGFVVQELVSNALLHGNLEVGGIDCEGGGGLEGFGERIEAALGDPGRAGRRVQISASVV
HHHHHHHHHHHHHHEECCCEEECEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEHEEC
GDRLEVAVEDDGPGFDHVDGHANPRRPHGLALLESMVSDLHTEEGGRRAIATMVIPTRQL
CCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECHHHH
RAEPLGLDDVRVLVVDDNLMNRVLMETLLRRMGVGQVETVESGEKGLAAIEREKPDLVLL
CCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHCCCCCEEEE
DVMMPGMDGFEMCRRLRRLHPLTELPVIFVTALDGPADRNACFAVGGSDMVAKPIDANEV
EECCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEECCCCCEECCCCHHHH
AARVGVHLRLGQAMDRLTAYQDRVHEELRAARGAQAALMPTSAELSSIRIRQGLVVDGRI
HHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHEEHHCCEEEECCC
ESSSELGGDFWTVLPAGPNQMFLLVADFTGHGPVAAFNVFRLHLLLSRLPRRMPTPSALL
CCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
EYLNLELKAVLRSGQFAAAFAALIDVEEGVMTYAGAASPPAVLVVDGEVRFLDADGPPLG
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHCCCCCCCEEEEECCEEEEEECCCCCCC
AFVDAEYEESRIALPRGAALLAYSDALVESQGSDGRMACDQETLREWVLGAQPGHSLADE
EEEECCCCCCEEECCCCCEEEEEHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHH
VLARFREKVPGEPPDDLTLVSIRRP
HHHHHHHHCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA