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The map label for this gene is kefB [H]

Identifier: 83312565

GI number: 83312565

Start: 3805718

End: 3807439

Strand: Reverse

Name: kefB [H]

Synonym: amb3466

Alternate gene names: 83312565

Gene position: 3807439-3805718 (Counterclockwise)

Preceding gene: 83312566

Following gene: 83312559

Centisome position: 76.65

GC content: 70.56

Gene sequence:

>1722_bases
TTGCCGCTATACTCCCCGGGACCAATGGGGAGTGATCGCGTGCACGGGTCCGAAGCGCTGTTCGATGTCTTGTCGCTGCT
GCTGGCGGCGGTGGTGCTGGTGCCGCTGTTCCAGCGGTTCGGCATCCCCTCGGTGCTGGCCTATCTGGCGGCGGGCGTGG
CGCTGGGTCCCTATACCCCCGGCCCGGTGGTCGACCTGGAGGTCACCCGGCCGCTGGCCGAATTCGGGGTGGTGTTCCTG
CTGTTCGCCATCGGCATGGAATTGCCGCTGTCGCGTCTTCGGACCATGCGCCGCTATATCCTGGGGCTGGGCCTGGCCCA
GGTGGTGGTCACCGCCCTGGCCATCGGCGGCATCAGCCATGCCCTCGGCCTGGGCAGCAGTGCCGCCCTGATCATCGGGC
TGACCCTGGCGTTCTCCTCCACCGCCACGGTGCTGGCCATCCTGGTGGAGCGCGGCGAGGCGGTGGACCATCACGGCCGC
ATCGCCGTGGCCGTGCTGATCTTCCAGGATCTGGCGGTGGTGCCGGTGCTGGTGCTGCTGCCCCTGCTGGCGGGGCAGGA
GCGGCACATGGCCGAGGCCCTGGCCCTGGCCGGGGGCAAGGTGGCGCTGGTCATGGCCGCCATCTTCGTGGTCGGGCGCT
TCCTGCTGCGTCCCGTCTACCGGTTCATCGCCACTGCCCGCAATCCCGAGGTGTTTACCGCCGCCAACCTGCTGTTGGTC
CTGGCCGTGGCCTGGGTGACCGGCGAGGCCGGCATGTCCATGGCCCTGGGCGCCTTCCTGGCCGGGCTGCTGCTGGCCGA
TTCCCGCTATCGCCATCAGGTGGAAGCCGATATCGAGCCGTTTCGCGGCCTGCTGCTGGGCCTGTTCTTCATGACCGTGG
GCATGTCCATCAACCTGCCCCTGGTGGCCGACCGGCTTGGGCTGGTCCTTGGCCTGACCCTGGGCACGGTGGCTGTCAAG
GCGGTGGTGCTGATCGGCCTGTGCCGTCTGATGAAGGTGGGGCTGGCCGACGGACTGCGGGTGGGGTTCCTGCTGGCCCA
GGGCGGCGAGTTCGCGTTCGTGGTGTTCGGCCGTGCCCTCGAACTGAAGCTTCTCGACGGCGGGCTGGGCCAGTCCCTGC
TGGCCAGCGTGGCCCTGTCCATGTTGCTGACCCCCGCCCTGGCGGCGCTGGGGCGGCGCTGGTCCCAGGGACTGGCGCCG
CAAGCCAGCGGCGAACCGATCCCCGAGACGGCGCGCCATCTGGCCGACCATGTGATCATCGCCGGCTATGGCCGGGTGGG
CCGCGCCATCGGCAGGCTGCTGGCCGAGCACGGCATTTCCTTCATCGCCCTCGACATGGATGTGGACCGGGTGGCCAAGG
CGCGCGAGGACGGCATTCCCGTCTATTACGGCGATGCCGGCCGCACCGGCATCCTGCGCTCGGTGGGCATCGACCGGGCG
CGGGCGGCGGTGATCACCGTCAACGAGGCCCGCCAGGCCGAGCGCGCCATCGGGTGCATCCGGCAGGCGGCGCCCCATCT
GCCCATCGTGGCCCGGGCCCGCGACCGCGCCCAGGAACAGCTTCTGGGCGATATCGGCGCCACCGCCGTCATTCCCGAGA
CGGTGGAGGCCAGCCTGCAGATGGCCGGCGCGGTGCTGCGCTCGGCGGGAATCGACGAGGACGCGGTGGAGCGCAGCCTG
AAGGGCTATCGCCGCCGGCGCTATGGCCGGGCGACGGAGTGA

Upstream 100 bases:

>100_bases
GCCCATTCCCTGGCGGACCGGGCCCAGTCGCTGCGGGCGGCGGTCACCGGCTTCCTGGTGGACGCCAAGACCGTCTGACA
GCGGTTCGACACGGGCGTTC

Downstream 100 bases:

>100_bases
GGTCGGCGGGCTCTTGCGGGGCTCTCGCCCCGGACCCCGGTTGGAGGTGAACCTCCAAACCTCCCTTCCTTCTCTTCATA
AGACATAAAGGGGGGCCGGG

Product: glutathione-regulated potassium-efflux system protein kefB

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 573; Mature: 572

Protein sequence:

>573_residues
MPLYSPGPMGSDRVHGSEALFDVLSLLLAAVVLVPLFQRFGIPSVLAYLAAGVALGPYTPGPVVDLEVTRPLAEFGVVFL
LFAIGMELPLSRLRTMRRYILGLGLAQVVVTALAIGGISHALGLGSSAALIIGLTLAFSSTATVLAILVERGEAVDHHGR
IAVAVLIFQDLAVVPVLVLLPLLAGQERHMAEALALAGGKVALVMAAIFVVGRFLLRPVYRFIATARNPEVFTAANLLLV
LAVAWVTGEAGMSMALGAFLAGLLLADSRYRHQVEADIEPFRGLLLGLFFMTVGMSINLPLVADRLGLVLGLTLGTVAVK
AVVLIGLCRLMKVGLADGLRVGFLLAQGGEFAFVVFGRALELKLLDGGLGQSLLASVALSMLLTPALAALGRRWSQGLAP
QASGEPIPETARHLADHVIIAGYGRVGRAIGRLLAEHGISFIALDMDVDRVAKAREDGIPVYYGDAGRTGILRSVGIDRA
RAAVITVNEARQAERAIGCIRQAAPHLPIVARARDRAQEQLLGDIGATAVIPETVEASLQMAGAVLRSAGIDEDAVERSL
KGYRRRRYGRATE

Sequences:

>Translated_573_residues
MPLYSPGPMGSDRVHGSEALFDVLSLLLAAVVLVPLFQRFGIPSVLAYLAAGVALGPYTPGPVVDLEVTRPLAEFGVVFL
LFAIGMELPLSRLRTMRRYILGLGLAQVVVTALAIGGISHALGLGSSAALIIGLTLAFSSTATVLAILVERGEAVDHHGR
IAVAVLIFQDLAVVPVLVLLPLLAGQERHMAEALALAGGKVALVMAAIFVVGRFLLRPVYRFIATARNPEVFTAANLLLV
LAVAWVTGEAGMSMALGAFLAGLLLADSRYRHQVEADIEPFRGLLLGLFFMTVGMSINLPLVADRLGLVLGLTLGTVAVK
AVVLIGLCRLMKVGLADGLRVGFLLAQGGEFAFVVFGRALELKLLDGGLGQSLLASVALSMLLTPALAALGRRWSQGLAP
QASGEPIPETARHLADHVIIAGYGRVGRAIGRLLAEHGISFIALDMDVDRVAKAREDGIPVYYGDAGRTGILRSVGIDRA
RAAVITVNEARQAERAIGCIRQAAPHLPIVARARDRAQEQLLGDIGATAVIPETVEASLQMAGAVLRSAGIDEDAVERSL
KGYRRRRYGRATE
>Mature_572_residues
PLYSPGPMGSDRVHGSEALFDVLSLLLAAVVLVPLFQRFGIPSVLAYLAAGVALGPYTPGPVVDLEVTRPLAEFGVVFLL
FAIGMELPLSRLRTMRRYILGLGLAQVVVTALAIGGISHALGLGSSAALIIGLTLAFSSTATVLAILVERGEAVDHHGRI
AVAVLIFQDLAVVPVLVLLPLLAGQERHMAEALALAGGKVALVMAAIFVVGRFLLRPVYRFIATARNPEVFTAANLLLVL
AVAWVTGEAGMSMALGAFLAGLLLADSRYRHQVEADIEPFRGLLLGLFFMTVGMSINLPLVADRLGLVLGLTLGTVAVKA
VVLIGLCRLMKVGLADGLRVGFLLAQGGEFAFVVFGRALELKLLDGGLGQSLLASVALSMLLTPALAALGRRWSQGLAPQ
ASGEPIPETARHLADHVIIAGYGRVGRAIGRLLAEHGISFIALDMDVDRVAKAREDGIPVYYGDAGRTGILRSVGIDRAR
AAVITVNEARQAERAIGCIRQAAPHLPIVARARDRAQEQLLGDIGATAVIPETVEASLQMAGAVLRSAGIDEDAVERSLK
GYRRRRYGRATE

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=348, Percent_Identity=26.7241379310345, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1789749, Length=555, Percent_Identity=41.0810810810811, Blast_Score=348, Evalue=4e-97,
Organism=Escherichia coli, GI1786232, Length=552, Percent_Identity=39.1304347826087, Blast_Score=320, Evalue=2e-88,
Organism=Escherichia coli, GI1786685, Length=533, Percent_Identity=30.5816135084428, Blast_Score=180, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 60387; Mature: 60256

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLYSPGPMGSDRVHGSEALFDVLSLLLAAVVLVPLFQRFGIPSVLAYLAAGVALGPYTP
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCC
GPVVDLEVTRPLAEFGVVFLLFAIGMELPLSRLRTMRRYILGLGLAQVVVTALAIGGISH
CCEEEEEHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGLGSSAALIIGLTLAFSSTATVLAILVERGEAVDHHGRIAVAVLIFQDLAVVPVLVLL
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
PLLAGQERHMAEALALAGGKVALVMAAIFVVGRFLLRPVYRFIATARNPEVFTAANLLLV
HHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LAVAWVTGEAGMSMALGAFLAGLLLADSRYRHQVEADIEPFRGLLLGLFFMTVGMSINLP
HHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCC
LVADRLGLVLGLTLGTVAVKAVVLIGLCRLMKVGLADGLRVGFLLAQGGEFAFVVFGRAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEECCCCEEEEEECCEE
ELKLLDGGLGQSLLASVALSMLLTPALAALGRRWSQGLAPQASGEPIPETARHLADHVII
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHEEE
AGYGRVGRAIGRLLAEHGISFIALDMDVDRVAKAREDGIPVYYGDAGRTGILRSVGIDRA
ECCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCEEECCCCCCCHHHHHCCCHH
RAAVITVNEARQAERAIGCIRQAAPHLPIVARARDRAQEQLLGDIGATAVIPETVEASLQ
HEEEEEEHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
MAGAVLRSAGIDEDAVERSLKGYRRRRYGRATE
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PLYSPGPMGSDRVHGSEALFDVLSLLLAAVVLVPLFQRFGIPSVLAYLAAGVALGPYTP
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCC
GPVVDLEVTRPLAEFGVVFLLFAIGMELPLSRLRTMRRYILGLGLAQVVVTALAIGGISH
CCEEEEEHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGLGSSAALIIGLTLAFSSTATVLAILVERGEAVDHHGRIAVAVLIFQDLAVVPVLVLL
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
PLLAGQERHMAEALALAGGKVALVMAAIFVVGRFLLRPVYRFIATARNPEVFTAANLLLV
HHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LAVAWVTGEAGMSMALGAFLAGLLLADSRYRHQVEADIEPFRGLLLGLFFMTVGMSINLP
HHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCC
LVADRLGLVLGLTLGTVAVKAVVLIGLCRLMKVGLADGLRVGFLLAQGGEFAFVVFGRAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEECCCCEEEEEECCEE
ELKLLDGGLGQSLLASVALSMLLTPALAALGRRWSQGLAPQASGEPIPETARHLADHVII
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHEEE
AGYGRVGRAIGRLLAEHGISFIALDMDVDRVAKAREDGIPVYYGDAGRTGILRSVGIDRA
ECCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCEEECCCCCCCHHHHHCCCHH
RAAVITVNEARQAERAIGCIRQAAPHLPIVARARDRAQEQLLGDIGATAVIPETVEASLQ
HEEEEEEHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
MAGAVLRSAGIDEDAVERSLKGYRRRRYGRATE
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA