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The map label for this gene is rubB [H]

Identifier: 83312526

GI number: 83312526

Start: 3755081

End: 3756244

Strand: Reverse

Name: rubB [H]

Synonym: amb3427

Alternate gene names: 83312526

Gene position: 3756244-3755081 (Counterclockwise)

Preceding gene: 83312527

Following gene: 83312525

Centisome position: 75.62

GC content: 72.25

Gene sequence:

>1164_bases
ATGGAGACCGCCGCCACCCAAAACGGCGTGGTTATCCTCGGCAGCGGGCTGGGGGGATATACCCTGGCCCGCGAGTTGAG
GAAGCTGGACGCCAACGTCGCCATCACCATCGTCACCGCGGATGGCGGGGAATCCTATTCCAAGCCCATGCTGTCCGCCG
CCTTCGCCCAGGGCAAGGACCCCACGACCCTGGTGCAGAAGAGCGCCGGCCAGATGGCGGCGGATCTATCGGCCACCATC
CTGGTCCGCCACCGGGTCGCCGCCATCCGCCGCGACGCCAAGGCGGTGGTGCTGCGCCGTCCCGACGGCGGCGAGGTGGA
GCTGGGTTATGGCCGGCTGGTGCTGGCCATCGGCGCCGATCCCCGGGCCTACACGGTCGAGGGCAGTGAGGCTGCCCGCA
TCCGCACCGTCAACGACCTGGACGACTACGCCGACTGGCGCGCCGCGCTGGGCGCCGGCGGGCGGGTCCTGCTGGTGGGC
GCCGGGCTGATCGGCTCGGAATTCGCCAATGATCTGGTGGGAGCCGGGTATGGAATCACCGTGGTCGACCCGGCGCCCTG
GCCGCTCGGCCGCCTGCTTCCCCAGGCGCTGGGCGAGGAGATGGCCAGGGCGCTGGGCGCGGCGGGGGTGACCTTCCACC
TGGGGCGTTCGGTGGCCCGGTTCTCGGCGGGGCGCGCCGTTCTTGACGACGGCACCGAACTCGCCTTCGACCTGGCGCTG
TCGGCCATCGGGCTGGTGCCGCGCATCCGGCTGGCGGCCGAGGCGGGGCTGAAGGTGGAGCGGGGCATCGTGGTCGACCG
GACCTTGCGGACCTCGGACCCGGCGATCTTCGCCATCGGCGATTGCGCCGAGACTCCCGCCGGGCCGTTGCCCTTCGTTC
TGCCGCTGATGGCCGAGGCCAAGACCCTGGCCGCCACCCTGGCCGGGACCGAGACCGAGTTGAAGCTGCCCGCCCTGCCG
GTGGTGGTCAAGACCCCGGCCCTTCCCATGGCGGTCTGCCCGCCCCCTCCCGGCGCGGCCGGCGAGTGGGTGGTGGAGGG
CGAGGGCCGCGACCGCAAGGCGCTGTTCGTCTCGCCCGGCGGCAAGGCCTTGGGCTTCGCGCTGTCCGGCACCCGCGTCA
ACGAGCGCCAGAGCCTGGCCAAGGAGATGCCTGACCTGTTGTGA

Upstream 100 bases:

>100_bases
CATCGCGCCTGGAACCCCCTTCGAAGCCATTCCCGACGACTGGTCTTGCCCCGATTGCGGCGTGTCCAAGTCGGACTTCG
AAGTCCAGGAGGACTGACCC

Downstream 100 bases:

>100_bases
CAAAGCGCAAATGATGCTTTTCTTGGCGGTACCAACAAACGTATTAGGGGGACCATTCCGATGAAGCTTCAGCTTGCCGC
CTTCGCCGCAGCCTTGGCCC

Product: NAD(FAD)-dependent dehydrogenase

Products: NA

Alternate protein names: RdxR [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
METAATQNGVVILGSGLGGYTLARELRKLDANVAITIVTADGGESYSKPMLSAAFAQGKDPTTLVQKSAGQMAADLSATI
LVRHRVAAIRRDAKAVVLRRPDGGEVELGYGRLVLAIGADPRAYTVEGSEAARIRTVNDLDDYADWRAALGAGGRVLLVG
AGLIGSEFANDLVGAGYGITVVDPAPWPLGRLLPQALGEEMARALGAAGVTFHLGRSVARFSAGRAVLDDGTELAFDLAL
SAIGLVPRIRLAAEAGLKVERGIVVDRTLRTSDPAIFAIGDCAETPAGPLPFVLPLMAEAKTLAATLAGTETELKLPALP
VVVKTPALPMAVCPPPPGAAGEWVVEGEGRDRKALFVSPGGKALGFALSGTRVNERQSLAKEMPDLL

Sequences:

>Translated_387_residues
METAATQNGVVILGSGLGGYTLARELRKLDANVAITIVTADGGESYSKPMLSAAFAQGKDPTTLVQKSAGQMAADLSATI
LVRHRVAAIRRDAKAVVLRRPDGGEVELGYGRLVLAIGADPRAYTVEGSEAARIRTVNDLDDYADWRAALGAGGRVLLVG
AGLIGSEFANDLVGAGYGITVVDPAPWPLGRLLPQALGEEMARALGAAGVTFHLGRSVARFSAGRAVLDDGTELAFDLAL
SAIGLVPRIRLAAEAGLKVERGIVVDRTLRTSDPAIFAIGDCAETPAGPLPFVLPLMAEAKTLAATLAGTETELKLPALP
VVVKTPALPMAVCPPPPGAAGEWVVEGEGRDRKALFVSPGGKALGFALSGTRVNERQSLAKEMPDLL
>Mature_387_residues
METAATQNGVVILGSGLGGYTLARELRKLDANVAITIVTADGGESYSKPMLSAAFAQGKDPTTLVQKSAGQMAADLSATI
LVRHRVAAIRRDAKAVVLRRPDGGEVELGYGRLVLAIGADPRAYTVEGSEAARIRTVNDLDDYADWRAALGAGGRVLLVG
AGLIGSEFANDLVGAGYGITVVDPAPWPLGRLLPQALGEEMARALGAAGVTFHLGRSVARFSAGRAVLDDGTELAFDLAL
SAIGLVPRIRLAAEAGLKVERGIVVDRTLRTSDPAIFAIGDCAETPAGPLPFVLPLMAEAKTLAATLAGTETELKLPALP
VVVKTPALPMAVCPPPPGAAGEWVVEGEGRDRKALFVSPGGKALGFALSGTRVNERQSLAKEMPDLL

Specific function: Involved in the hydrocarbon hydroxylating system, which transfers electrons from NADH to rubredoxin reductase and then through rubredoxin to alkane 1 monooxygenase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=289, Percent_Identity=26.2975778546713, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI21389617, Length=289, Percent_Identity=26.2975778546713, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI226437568, Length=289, Percent_Identity=26.2975778546713, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI1789065, Length=381, Percent_Identity=34.3832020997375, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1789765, Length=312, Percent_Identity=28.2051282051282, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788892, Length=279, Percent_Identity=27.5985663082437, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17559934, Length=285, Percent_Identity=26.3157894736842, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.18.1.1 [H]

Molecular weight: Translated: 40010; Mature: 40010

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METAATQNGVVILGSGLGGYTLARELRKLDANVAITIVTADGGESYSKPMLSAAFAQGKD
CCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCC
PTTLVQKSAGQMAADLSATILVRHRVAAIRRDAKAVVLRRPDGGEVELGYGRLVLAIGAD
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCEEEEEECCC
PRAYTVEGSEAARIRTVNDLDDYADWRAALGAGGRVLLVGAGLIGSEFANDLVGAGYGIT
CCEEEECCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCEE
VVDPAPWPLGRLLPQALGEEMARALGAAGVTFHLGRSVARFSAGRAVLDDGTELAFDLAL
EECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCEEECCCCHHHHHHHH
SAIGLVPRIRLAAEAGLKVERGIVVDRTLRTSDPAIFAIGDCAETPAGPLPFVLPLMAEA
HHHHHHHHHHHHHHCCCEEECCEEEEEEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHH
KTLAATLAGTETELKLPALPVVVKTPALPMAVCPPPPGAAGEWVVEGEGRDRKALFVSPG
HHHHHHHCCCCCEEECCCCCEEEECCCCCEEECCCCCCCCCCEEEECCCCCCEEEEECCC
GKALGFALSGTRVNERQSLAKEMPDLL
CCEEEEEEECCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
METAATQNGVVILGSGLGGYTLARELRKLDANVAITIVTADGGESYSKPMLSAAFAQGKD
CCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCC
PTTLVQKSAGQMAADLSATILVRHRVAAIRRDAKAVVLRRPDGGEVELGYGRLVLAIGAD
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCEEEEEECCC
PRAYTVEGSEAARIRTVNDLDDYADWRAALGAGGRVLLVGAGLIGSEFANDLVGAGYGIT
CCEEEECCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCEE
VVDPAPWPLGRLLPQALGEEMARALGAAGVTFHLGRSVARFSAGRAVLDDGTELAFDLAL
EECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCEEECCCCHHHHHHHH
SAIGLVPRIRLAAEAGLKVERGIVVDRTLRTSDPAIFAIGDCAETPAGPLPFVLPLMAEA
HHHHHHHHHHHHHHCCCEEECCEEEEEEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHH
KTLAATLAGTETELKLPALPVVVKTPALPMAVCPPPPGAAGEWVVEGEGRDRKALFVSPG
HHHHHHHCCCCCEEECCCCCEEEECCCCCEEECCCCCCCCCCEEEECCCCCCEEEEECCC
GKALGFALSGTRVNERQSLAKEMPDLL
CCEEEEEEECCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA