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The map label for this gene is nemA [H]

Identifier: 83312404

GI number: 83312404

Start: 3586869

End: 3587927

Strand: Reverse

Name: nemA [H]

Synonym: amb3305

Alternate gene names: 83312404

Gene position: 3587927-3586869 (Counterclockwise)

Preceding gene: 83312405

Following gene: 83312401

Centisome position: 72.23

GC content: 68.27

Gene sequence:

>1059_bases
ATGACCAAAGATCTCTTCCAGCCCCTGCGCTTGGGCGAACTTACCCTGCCGAGCCGCATCGCCATGGCCCCCATGACCCG
CTCACGGGCCGATGACGACGGCGTGCAGCCCGAGATCGCCGCCACCTATTACGGCCAGCGCGCCGGGGCTGGCCTGATCA
TCTCCGAGGGCATCTACCCTTCGGCCATGGGCAAGGGCTATGTCCGCACCCCGGGCCTTCATACCGACGCCCAGGTGGCC
GCCTGGAGCAAGGTCACCAAGGCGGTGCACGACAAGGGCGGGCGCATCTTCGCCCAGCTGATGCACACCGGCCGGGTCGC
CGTTCCCGCCTTCCTGCCCGGTGGCGCGCTGCCGGTGGCGCCCTCGGCTATCCGCGCCGAGGGTCAGGCCTACACCGATC
AGGGCATGGTGCCGATGGTCACGCCGCGCGCCCTGGAGACCACAGAGATCGCCGGTGTGATCGCCGAATACCGAGACGCC
ACCCGCCGCGCTCTGGAGGCCGGCTTCGACGGCGTTGAACTGCATGCGGCATCGGGGTACCTGCCGGAGCAGTTCCTGTC
GTCCAAGAGCAACCGCCGCACCGATGGCTATGGCGGGTCCATCGCCAACCGAGTCCGCTTTGTGGTCGAGGTGCTGGAGG
CCATGGCGGCGGTGGCGGGTTCCGGCCGCGTCGGCATCAAGATCTCGCCGGAGATGAACTTCAACGACGTGGCGGACGAC
ACCCCGCAGGAGACCTACCGCCACCTGGTCGAGCGCATCTCGTCCCTGAACCTCGCCTATCTGCACGTGGCGCTGTTCGG
CACCCCGACCGATTATCATGGCCTGCTGCGCCCCCTGTTCAAGGGAGCCTATCTGGCCGGCGGCGGCTTTACCGGTGACA
CCGCCGAAGCCGCCCTGGCCCAGGGCCGCGCCGATGCCATCGTCTTTGGTGCCGCCTTCATCGCCAACCCCGATCTGCCC
AAGCGGCTGGCGGAGGGGTTGCCCCTGGCCGAACCCGACAAGGCGACTTTCTACGCGCCGGGACCGCAGGGCTACATCGA
TTACCCACCGGCGGTGTGA

Upstream 100 bases:

>100_bases
AGTTGATCACCCTGTTGTTCACCGATGTCTTCCCGGCCCTGGGTGCCGGCATCGTCTAGGTCAGAAATCAATCGCAGATC
ACGAATACGGAGCCCCCATC

Downstream 100 bases:

>100_bases
CGGAGTTGGGATGAAGATCGCCCGCGACGGTGGCGCGGATATCTAAAAGAGGCGGCCGGCCAGGACATCCCCCTGGCCGG
CCGAGTTTCGTCTTGGCAAT

Product: NADH:flavin oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MTKDLFQPLRLGELTLPSRIAMAPMTRSRADDDGVQPEIAATYYGQRAGAGLIISEGIYPSAMGKGYVRTPGLHTDAQVA
AWSKVTKAVHDKGGRIFAQLMHTGRVAVPAFLPGGALPVAPSAIRAEGQAYTDQGMVPMVTPRALETTEIAGVIAEYRDA
TRRALEAGFDGVELHAASGYLPEQFLSSKSNRRTDGYGGSIANRVRFVVEVLEAMAAVAGSGRVGIKISPEMNFNDVADD
TPQETYRHLVERISSLNLAYLHVALFGTPTDYHGLLRPLFKGAYLAGGGFTGDTAEAALAQGRADAIVFGAAFIANPDLP
KRLAEGLPLAEPDKATFYAPGPQGYIDYPPAV

Sequences:

>Translated_352_residues
MTKDLFQPLRLGELTLPSRIAMAPMTRSRADDDGVQPEIAATYYGQRAGAGLIISEGIYPSAMGKGYVRTPGLHTDAQVA
AWSKVTKAVHDKGGRIFAQLMHTGRVAVPAFLPGGALPVAPSAIRAEGQAYTDQGMVPMVTPRALETTEIAGVIAEYRDA
TRRALEAGFDGVELHAASGYLPEQFLSSKSNRRTDGYGGSIANRVRFVVEVLEAMAAVAGSGRVGIKISPEMNFNDVADD
TPQETYRHLVERISSLNLAYLHVALFGTPTDYHGLLRPLFKGAYLAGGGFTGDTAEAALAQGRADAIVFGAAFIANPDLP
KRLAEGLPLAEPDKATFYAPGPQGYIDYPPAV
>Mature_351_residues
TKDLFQPLRLGELTLPSRIAMAPMTRSRADDDGVQPEIAATYYGQRAGAGLIISEGIYPSAMGKGYVRTPGLHTDAQVAA
WSKVTKAVHDKGGRIFAQLMHTGRVAVPAFLPGGALPVAPSAIRAEGQAYTDQGMVPMVTPRALETTEIAGVIAEYRDAT
RRALEAGFDGVELHAASGYLPEQFLSSKSNRRTDGYGGSIANRVRFVVEVLEAMAAVAGSGRVGIKISPEMNFNDVADDT
PQETYRHLVERISSLNLAYLHVALFGTPTDYHGLLRPLFKGAYLAGGGFTGDTAEAALAQGRADAIVFGAAFIANPDLPK
RLAEGLPLAEPDKATFYAPGPQGYIDYPPAV

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=366, Percent_Identity=43.1693989071038, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1789463, Length=220, Percent_Identity=36.3636363636364, Blast_Score=119, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17559802, Length=273, Percent_Identity=31.5018315018315, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17565138, Length=276, Percent_Identity=30.0724637681159, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17559804, Length=273, Percent_Identity=31.1355311355311, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=268, Percent_Identity=30.5970149253731, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17564188, Length=272, Percent_Identity=30.1470588235294, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17540738, Length=279, Percent_Identity=30.8243727598566, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6325086, Length=373, Percent_Identity=33.7801608579088, Blast_Score=189, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6321973, Length=373, Percent_Identity=33.5120643431635, Blast_Score=189, Evalue=7e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 37392; Mature: 37261

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKDLFQPLRLGELTLPSRIAMAPMTRSRADDDGVQPEIAATYYGQRAGAGLIISEGIYP
CCHHHHHHHHCCCCCCCCHHHHCCCHHCCCCCCCCCCCHHHHHCCHHCCCCEEEECCCCH
SAMGKGYVRTPGLHTDAQVAAWSKVTKAVHDKGGRIFAQLMHTGRVAVPAFLPGGALPVA
HHCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEECCCCCCCCC
PSAIRAEGQAYTDQGMVPMVTPRALETTEIAGVIAEYRDATRRALEAGFDGVELHAASGY
CHHHHCCCCEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
LPEQFLSSKSNRRTDGYGGSIANRVRFVVEVLEAMAAVAGSGRVGIKISPEMNFNDVADD
CHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCC
TPQETYRHLVERISSLNLAYLHVALFGTPTDYHGLLRPLFKGAYLAGGGFTGDTAEAALA
CHHHHHHHHHHHHHHCCEEEEEEEEECCCCHHHHHHHHHHCCCEEECCCCCCCHHHHHHH
QGRADAIVFGAAFIANPDLPKRLAEGLPLAEPDKATFYAPGPQGYIDYPPAV
CCCCCEEEEEHHHHCCCCHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
TKDLFQPLRLGELTLPSRIAMAPMTRSRADDDGVQPEIAATYYGQRAGAGLIISEGIYP
CHHHHHHHHCCCCCCCCHHHHCCCHHCCCCCCCCCCCHHHHHCCHHCCCCEEEECCCCH
SAMGKGYVRTPGLHTDAQVAAWSKVTKAVHDKGGRIFAQLMHTGRVAVPAFLPGGALPVA
HHCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEECCCCCCCCC
PSAIRAEGQAYTDQGMVPMVTPRALETTEIAGVIAEYRDATRRALEAGFDGVELHAASGY
CHHHHCCCCEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
LPEQFLSSKSNRRTDGYGGSIANRVRFVVEVLEAMAAVAGSGRVGIKISPEMNFNDVADD
CHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCC
TPQETYRHLVERISSLNLAYLHVALFGTPTDYHGLLRPLFKGAYLAGGGFTGDTAEAALA
CHHHHHHHHHHHHHHCCEEEEEEEEECCCCHHHHHHHHHHCCCEEECCCCCCCHHHHHHH
QGRADAIVFGAAFIANPDLPKRLAEGLPLAEPDKATFYAPGPQGYIDYPPAV
CCCCCEEEEEHHHHCCCCHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]