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The map label for this gene is ruvA

Identifier: 83312317

GI number: 83312317

Start: 3483969

End: 3484607

Strand: Reverse

Name: ruvA

Synonym: amb3218

Alternate gene names: 83312317

Gene position: 3484607-3483969 (Counterclockwise)

Preceding gene: 83312318

Following gene: 83312316

Centisome position: 70.15

GC content: 69.33

Gene sequence:

>639_bases
ATGATCGCCAAGCTGAAGGGCCTGATCGATTCCCTCGGCGACGACTGGGCCGTGGTGGATTGCAACGGCGTCGGCTATCT
GGTGGCCTGCTCGTCCAAGACCCTGGCGCGGCTGGAGACCGGCACCGCCGCCGCCCTGTTCGTCGAGACCCAGGTGCGGG
AAGACGCCATCTCGCTGTTCGGCTTTTTGGAAACCGGCGAGCGCGACTGGTTCCGGCTGCTGACCACGGTGCAGGGGGTT
GGGGCCAAGGTAGCCCTGGCCATTCTGTCGGTGGCCTCGCCGGACCAGCTGCTGCAGATCATCGCGGCCCAGGACAAGGC
CGGGCTGACCCGGGCCAACGGCGTCGGGCCGAAACTGGCGGTGCGTATCCTCACCGAGTTGAAGGACAAGGCCGGCAAGA
TCGCCCTGGGGGGCTTCTCTCCCGGTGGGATCAAGGACGCGCTGTCGGCTTCGGCGCCGCTGCCCGCCGCCTCGGGCCGC
ATGGAGGACGCCGTCTCCGCCCTGGTCAATCTGGGCTACAAGCGCCTGGAGGCCTTCCAGGCGGTGGGCGAGACCGCCCG
CGAGCTGGGGGACGAGGCGGACTCGTCCGCCCTGATCCGCGCCGCCCTGAAGCACCTGGGCAAGGGGCTGCTGGGATGA

Upstream 100 bases:

>100_bases
GTTTGGCCGCCACGGCCGATGCCGCCGATGCCCTGGCGGTGGCCATCTGCCACGCCCATCACCGGGGCACGCAGCAGAAG
CTGGCGCGGGCGGTGGCGCG

Downstream 100 bases:

>100_bases
GGCGGCAGGCATCCGCCATCCTCGGGGCTTCCGCCCCGGTCCCCGACCGGGAGGCCATGCCTCCCCGTCCCACCCTTGGC
TTGAAAGTGAAGGGGGTATG

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MIAKLKGLIDSLGDDWAVVDCNGVGYLVACSSKTLARLETGTAAALFVETQVREDAISLFGFLETGERDWFRLLTTVQGV
GAKVALAILSVASPDQLLQIIAAQDKAGLTRANGVGPKLAVRILTELKDKAGKIALGGFSPGGIKDALSASAPLPAASGR
MEDAVSALVNLGYKRLEAFQAVGETARELGDEADSSALIRAALKHLGKGLLG

Sequences:

>Translated_212_residues
MIAKLKGLIDSLGDDWAVVDCNGVGYLVACSSKTLARLETGTAAALFVETQVREDAISLFGFLETGERDWFRLLTTVQGV
GAKVALAILSVASPDQLLQIIAAQDKAGLTRANGVGPKLAVRILTELKDKAGKIALGGFSPGGIKDALSASAPLPAASGR
MEDAVSALVNLGYKRLEAFQAVGETARELGDEADSSALIRAALKHLGKGLLG
>Mature_212_residues
MIAKLKGLIDSLGDDWAVVDCNGVGYLVACSSKTLARLETGTAAALFVETQVREDAISLFGFLETGERDWFRLLTTVQGV
GAKVALAILSVASPDQLLQIIAAQDKAGLTRANGVGPKLAVRILTELKDKAGKIALGGFSPGGIKDALSASAPLPAASGR
MEDAVSALVNLGYKRLEAFQAVGETARELGDEADSSALIRAALKHLGKGLLG

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=205, Percent_Identity=36.5853658536585, Blast_Score=109, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_MAGSA (Q2W2A3)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_422581.1
- HSSP:   P40832
- ProteinModelPortal:   Q2W2A3
- SMR:   Q2W2A3
- STRING:   Q2W2A3
- GeneID:   3804976
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb3218
- NMPDR:   fig|342108.5.peg.2887
- eggNOG:   COG0632
- HOGENOM:   HBG635309
- OMA:   LFGFAQK
- PhylomeDB:   Q2W2A3
- ProtClustDB:   PRK00116
- BioCyc:   MMAG342108:AMB3218-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 21932; Mature: 21932

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAKLKGLIDSLGDDWAVVDCNGVGYLVACSSKTLARLETGTAAALFVETQVREDAISLF
CCHHHHHHHHHCCCCEEEEEECCCEEEEEECCCHHHHHHCCCCEEEEEHHHHHHHHHHHH
GFLETGERDWFRLLTTVQGVGAKVALAILSVASPDQLLQIIAAQDKAGLTRANGVGPKLA
HHHHCCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHH
VRILTELKDKAGKIALGGFSPGGIKDALSASAPLPAASGRMEDAVSALVNLGYKRLEAFQ
HHHHHHHHHHCCEEEECCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AVGETARELGDEADSSALIRAALKHLGKGLLG
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIAKLKGLIDSLGDDWAVVDCNGVGYLVACSSKTLARLETGTAAALFVETQVREDAISLF
CCHHHHHHHHHCCCCEEEEEECCCEEEEEECCCHHHHHHCCCCEEEEEHHHHHHHHHHHH
GFLETGERDWFRLLTTVQGVGAKVALAILSVASPDQLLQIIAAQDKAGLTRANGVGPKLA
HHHHCCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHH
VRILTELKDKAGKIALGGFSPGGIKDALSASAPLPAASGRMEDAVSALVNLGYKRLEAFQ
HHHHHHHHHHCCEEEECCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AVGETARELGDEADSSALIRAALKHLGKGLLG
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA