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The map label for this gene is ruvC

Identifier: 83312318

GI number: 83312318

Start: 3484604

End: 3485104

Strand: Reverse

Name: ruvC

Synonym: amb3219

Alternate gene names: 83312318

Gene position: 3485104-3484604 (Counterclockwise)

Preceding gene: 162319783

Following gene: 83312317

Centisome position: 70.16

GC content: 67.47

Gene sequence:

>501_bases
ATGAGAATCCTGGGGCTTGATCCTGGGTTGCGCAATACCGGCTGGGGCATCATCGATGCGGTCGACAACCGTCTGCGCCA
TGTGGCCGACGGAGTGATCCGTCCCGATGCCTCCGCCGCTTTGGCCGAGCGCCTGGTTCAGCTCCATGACGGCATCATGG
CGGTGATCGCCGATTTCTCCCCCGACGAGGCGGCGGTGGAGGAGACTTTCGTCAACATGAACCCCGCCAGCACCCTGAAG
CTGGGCCAGGCCCGGGGCGTGGTGCTGCTGGTTCCCGCCAAATCCGGCCTGCCGGTGGGCGAATACGCCGCCACCCTGGT
CAAGCAATCGGTGGTGGGAACCGGGCGCGCCGCCAAGGAACAGGTGGGCATGATGGTCAAGACCTTGCTGCCCGGCTGTT
TGGCCGCCACGGCCGATGCCGCCGATGCCCTGGCGGTGGCCATCTGCCACGCCCATCACCGGGGCACGCAGCAGAAGCTG
GCGCGGGCGGTGGCGCGATGA

Upstream 100 bases:

>100_bases
CCCTGCTGAAACTGCTGGACACCCTGGAGGACAACGACGACGTCCAGCGGGTCCAGGCCAATTTCGAGATCCCCGACGAC
ATCATGGAAAAGCTCGGCTG

Downstream 100 bases:

>100_bases
TCGCCAAGCTGAAGGGCCTGATCGATTCCCTCGGCGACGACTGGGCCGTGGTGGATTGCAACGGCGTCGGCTATCTGGTG
GCCTGCTCGTCCAAGACCCT

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MRILGLDPGLRNTGWGIIDAVDNRLRHVADGVIRPDASAALAERLVQLHDGIMAVIADFSPDEAAVEETFVNMNPASTLK
LGQARGVVLLVPAKSGLPVGEYAATLVKQSVVGTGRAAKEQVGMMVKTLLPGCLAATADAADALAVAICHAHHRGTQQKL
ARAVAR

Sequences:

>Translated_166_residues
MRILGLDPGLRNTGWGIIDAVDNRLRHVADGVIRPDASAALAERLVQLHDGIMAVIADFSPDEAAVEETFVNMNPASTLK
LGQARGVVLLVPAKSGLPVGEYAATLVKQSVVGTGRAAKEQVGMMVKTLLPGCLAATADAADALAVAICHAHHRGTQQKL
ARAVAR
>Mature_166_residues
MRILGLDPGLRNTGWGIIDAVDNRLRHVADGVIRPDASAALAERLVQLHDGIMAVIADFSPDEAAVEETFVNMNPASTLK
LGQARGVVLLVPAKSGLPVGEYAATLVKQSVVGTGRAAKEQVGMMVKTLLPGCLAATADAADALAVAICHAHHRGTQQKL
ARAVAR

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=162, Percent_Identity=43.8271604938272, Blast_Score=113, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_MAGSA (Q2W2A2)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_422582.1
- ProteinModelPortal:   Q2W2A2
- SMR:   Q2W2A2
- STRING:   Q2W2A2
- GeneID:   3804977
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb3219
- NMPDR:   fig|342108.5.peg.2888
- eggNOG:   COG0817
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- PhylomeDB:   Q2W2A2
- ProtClustDB:   PRK00039
- BioCyc:   MMAG342108:AMB3219-MONOMER
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 17302; Mature: 17302

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILGLDPGLRNTGWGIIDAVDNRLRHVADGVIRPDASAALAERLVQLHDGIMAVIADFS
CEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
PDEAAVEETFVNMNPASTLKLGQARGVVLLVPAKSGLPVGEYAATLVKQSVVGTGRAAKE
CCHHHHHHHHHCCCCCHHHEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHH
QVGMMVKTLLPGCLAATADAADALAVAICHAHHRGTQQKLARAVAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MRILGLDPGLRNTGWGIIDAVDNRLRHVADGVIRPDASAALAERLVQLHDGIMAVIADFS
CEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
PDEAAVEETFVNMNPASTLKLGQARGVVLLVPAKSGLPVGEYAATLVKQSVVGTGRAAKE
CCHHHHHHHHHCCCCCHHHEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHH
QVGMMVKTLLPGCLAATADAADALAVAICHAHHRGTQQKLARAVAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA