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The map label for this gene is ruvB

Identifier: 83312315

GI number: 83312315

Start: 3482645

End: 3483688

Strand: Reverse

Name: ruvB

Synonym: amb3216

Alternate gene names: 83312315

Gene position: 3483688-3482645 (Counterclockwise)

Preceding gene: 83312316

Following gene: 83312314

Centisome position: 70.13

GC content: 67.53

Gene sequence:

>1044_bases
ATGACCTCCCGCGTGGTCTCGCCCGAGCAGAACCCCAATGATGCCGAGATGTCGCTGCGGCCGCAGTCGCTGGACGATTT
CGTCGGCCAGCGCCAGGTGTGCGAGAACCTGAAGGTGTTCATCAGCGCCGCCAGGGCGCGGGGCGAGGCTCTGGATCACG
TGCTGTTCCACGGCCCGCCCGGCCTGGGCAAGACCACTCTGGCGCAGATCATGGCTCGCGAACTGGGAGTAGGCTTTCGC
GCCACCTCGGGTCCGGTGATCCAGCGGGCGGGCGATCTGGCCGCCTTGCTGACCAATCTCGAGCCCCGTGACGTTCTGTT
CATCGACGAGATCCATCGCCTTAATCCTGCCATAGAGGAAGTTCTCTATCCTGCGATGGAGGATTTTCAGTTGGATCTGA
TCATCGGCGAAGGTCCGGCGGCCCGTTCGGTCCGCATCGAGCTTCCCCCCTTCACCCTGGTGGGGGCGACCACCCGTTCG
GGGCTGCTGACCACGCCACTGCGGGAACGCTTCGGCATTCCCTGCCGCATGAATTTCTACGAGCCGGCCGAGCTGGAGGC
CATCGTGTCGCGTGGCGCCCGTGTGCTGGGCTTCGCCCTGACCCCCGATGGCGCCGCCGAGGTGGCTCGCCGCTCCCGGG
GCACGCCCCGCGTCGCTGGGCGTCTGCTGCGCCGGGTGCGCGACTTCGCCGTCGTGGCCGGGCGCTCGCCGGTGGACGCC
CTGGTGGCCGATGCCGCCTTGAACCGGCTGGAGGTGGACCGCATCGGCCTGGATGCCATGGATCGCCGCTATCTGCGCTG
CATCGCCGAGAATTACGGCGGCGGGCCGGTGGGGGTGGAAACCCTGGCCGCCGCGCTGTCGGAAAGCCGCGACACCCTGG
AAGAGGTGGTCGAGCCCTATCTGCTGCAGCAGGGCATGATCCAGCGCACGCCGCGTGGCCGCATGCTGTCGGCCTCCGGC
TTCAAGCATATCGGCCTCAATCCCCCCAAGGATCTGCTGGTGCAGCTCGACCTGCTGACCCGCATGGATGAGGAGGGGGA
ATGA

Upstream 100 bases:

>100_bases
ACGAGCGCCGCGAGGAGTTGCGGCCAAGGGCCGGCGCGGCTTATGCCGCGAAAGCCAAGCGAGCCGCAGGTGAGCGCCCG
GCGATTGAGGGGCATGTGAC

Downstream 100 bases:

>100_bases
TGGTGATGGCGACAGTCATCCCGACGACCAACGGGAGGAGGGATCTCATCCTGCTCCGCCATGTCGGGGTCGGCATCATC
GTTCCAGGCGGAGATCCCTC

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MTSRVVSPEQNPNDAEMSLRPQSLDDFVGQRQVCENLKVFISAARARGEALDHVLFHGPPGLGKTTLAQIMARELGVGFR
ATSGPVIQRAGDLAALLTNLEPRDVLFIDEIHRLNPAIEEVLYPAMEDFQLDLIIGEGPAARSVRIELPPFTLVGATTRS
GLLTTPLRERFGIPCRMNFYEPAELEAIVSRGARVLGFALTPDGAAEVARRSRGTPRVAGRLLRRVRDFAVVAGRSPVDA
LVADAALNRLEVDRIGLDAMDRRYLRCIAENYGGGPVGVETLAAALSESRDTLEEVVEPYLLQQGMIQRTPRGRMLSASG
FKHIGLNPPKDLLVQLDLLTRMDEEGE

Sequences:

>Translated_347_residues
MTSRVVSPEQNPNDAEMSLRPQSLDDFVGQRQVCENLKVFISAARARGEALDHVLFHGPPGLGKTTLAQIMARELGVGFR
ATSGPVIQRAGDLAALLTNLEPRDVLFIDEIHRLNPAIEEVLYPAMEDFQLDLIIGEGPAARSVRIELPPFTLVGATTRS
GLLTTPLRERFGIPCRMNFYEPAELEAIVSRGARVLGFALTPDGAAEVARRSRGTPRVAGRLLRRVRDFAVVAGRSPVDA
LVADAALNRLEVDRIGLDAMDRRYLRCIAENYGGGPVGVETLAAALSESRDTLEEVVEPYLLQQGMIQRTPRGRMLSASG
FKHIGLNPPKDLLVQLDLLTRMDEEGE
>Mature_346_residues
TSRVVSPEQNPNDAEMSLRPQSLDDFVGQRQVCENLKVFISAARARGEALDHVLFHGPPGLGKTTLAQIMARELGVGFRA
TSGPVIQRAGDLAALLTNLEPRDVLFIDEIHRLNPAIEEVLYPAMEDFQLDLIIGEGPAARSVRIELPPFTLVGATTRSG
LLTTPLRERFGIPCRMNFYEPAELEAIVSRGARVLGFALTPDGAAEVARRSRGTPRVAGRLLRRVRDFAVVAGRSPVDAL
VADAALNRLEVDRIGLDAMDRRYLRCIAENYGGGPVGVETLAAALSESRDTLEEVVEPYLLQQGMIQRTPRGRMLSASGF
KHIGLNPPKDLLVQLDLLTRMDEEGE

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=320, Percent_Identity=60.625, Blast_Score=407, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_MAGSA (Q2W2A5)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_422579.1
- ProteinModelPortal:   Q2W2A5
- SMR:   Q2W2A5
- STRING:   Q2W2A5
- GeneID:   3804974
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb3216
- NMPDR:   fig|342108.5.peg.2886
- eggNOG:   COG2255
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- PhylomeDB:   Q2W2A5
- ProtClustDB:   PRK00080
- BioCyc:   MMAG342108:AMB3216-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 37936; Mature: 37805

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRVVSPEQNPNDAEMSLRPQSLDDFVGQRQVCENLKVFISAARARGEALDHVLFHGPP
CCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GLGKTTLAQIMARELGVGFRATSGPVIQRAGDLAALLTNLEPRDVLFIDEIHRLNPAIEE
CCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHCCHHHHH
VLYPAMEDFQLDLIIGEGPAARSVRIELPPFTLVGATTRSGLLTTPLRERFGIPCRMNFY
HHHHHHHCCEEEEEECCCCCCCEEEEECCCEEEEECCCCCCCCCCCHHHHCCCCEEECCC
EPAELEAIVSRGARVLGFALTPDGAAEVARRSRGTPRVAGRLLRRVRDFAVVAGRSPVDA
CCHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
LVADAALNRLEVDRIGLDAMDRRYLRCIAENYGGGPVGVETLAAALSESRDTLEEVVEPY
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LLQQGMIQRTPRGRMLSASGFKHIGLNPPKDLLVQLDLLTRMDEEGE
HHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TSRVVSPEQNPNDAEMSLRPQSLDDFVGQRQVCENLKVFISAARARGEALDHVLFHGPP
CCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GLGKTTLAQIMARELGVGFRATSGPVIQRAGDLAALLTNLEPRDVLFIDEIHRLNPAIEE
CCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHCCHHHHH
VLYPAMEDFQLDLIIGEGPAARSVRIELPPFTLVGATTRSGLLTTPLRERFGIPCRMNFY
HHHHHHHCCEEEEEECCCCCCCEEEEECCCEEEEECCCCCCCCCCCHHHHCCCCEEECCC
EPAELEAIVSRGARVLGFALTPDGAAEVARRSRGTPRVAGRLLRRVRDFAVVAGRSPVDA
CCHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
LVADAALNRLEVDRIGLDAMDRRYLRCIAENYGGGPVGVETLAAALSESRDTLEEVVEPY
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LLQQGMIQRTPRGRMLSASGFKHIGLNPPKDLLVQLDLLTRMDEEGE
HHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA