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The map label for this gene is thiDE [H]

Identifier: 83312296

GI number: 83312296

Start: 3466086

End: 3466898

Strand: Reverse

Name: thiDE [H]

Synonym: amb3197

Alternate gene names: 83312296

Gene position: 3466898-3466086 (Counterclockwise)

Preceding gene: 83312298

Following gene: 83312295

Centisome position: 69.8

GC content: 70.6

Gene sequence:

>813_bases
TTGGCCATGCGAGGAAGGGTTCTGATCGTCGCCGGTTCTGATTCGGGTGGCGGTGCCGGTATCCAGGCCGACATCAAGGC
GGTGACCTGCCTGGGGGGCTTTGCCGCAACGGCGGTGACCGCCCTGACCGCCCAGAACACCCTGGGGGTGAGCGGCATCC
ACGAGGTGCCTCCCGATTTCGTGCGGGCGCAGATGGCGGCCGTCCTCAGCGACATCGGCGCCGACTGCATCAAGACCGGC
ATGCTGGCCTCGGCTGCCATCGTCGAAACGGTGGCCCTGGCGCTGGAGGAGCTGGCTCCCGCCGTGCCCCTGGTGCTTGA
TCCGGTGATGGTGGCCAAGGGCGGCGCCGCTTTGCTGGCCGACAGCGCCGCCGGAGCGCTGGTCAAGCGGTTGGTGCCGC
GCGCCCGGCTGCTGACACCCAATATCCCCGAGGCCGAGGTGCTGTTGGGGCGCGCCATCAAGGGCGCCGACGGCATGGAG
GGCGCGGCGCGGGACCTGCTGGCGTTGGGACCGAAGGCCGTGCTGCTCAAGGGCGGCCATCTGGAGGGTGACCATCTGGT
GGATGTGCTGGCCGAGGCGGGGGGCGGCCTCCGCCGCTTCACCGGGCGGCGCATCGCCAGCCGTTCGACCCATGGCACCG
GCTGTACCCTGGCGTCAGCCATTGCCGCCGGGCTGGCCCAGGGCTTGGGACTAGTCGAGGCGGTGGAGCGGGCCAGGGCC
TATCTGGTCAGGGCCATCGAGACCGCCCCCGGCCTGGGAAGCGGCCACGGCCCCCTCAACCATGGGCACACGGTCAAGGA
GTTTCAGGCATGA

Upstream 100 bases:

>100_bases
TTATTTTAGCTGCCGCTACAAAATGTCACCCGAATGCAGCTTATGGGTATCCCTATTAAAAATGTGTCAAAAGCATTAAA
TCTCTTGATTGAAGAGGGTC

Downstream 100 bases:

>100_bases
GCGCGCTTCCCTTCTGGCACGTGGACGCCTTTGTCGAGGACGCGCCGTTCTCCGGAAATCCGGCGGCGGTGGTGGTGCTG
CCCCAATGGCCCGACGACGC

Product: hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MAMRGRVLIVAGSDSGGGAGIQADIKAVTCLGGFAATAVTALTAQNTLGVSGIHEVPPDFVRAQMAAVLSDIGADCIKTG
MLASAAIVETVALALEELAPAVPLVLDPVMVAKGGAALLADSAAGALVKRLVPRARLLTPNIPEAEVLLGRAIKGADGME
GAARDLLALGPKAVLLKGGHLEGDHLVDVLAEAGGGLRRFTGRRIASRSTHGTGCTLASAIAAGLAQGLGLVEAVERARA
YLVRAIETAPGLGSGHGPLNHGHTVKEFQA

Sequences:

>Translated_270_residues
MAMRGRVLIVAGSDSGGGAGIQADIKAVTCLGGFAATAVTALTAQNTLGVSGIHEVPPDFVRAQMAAVLSDIGADCIKTG
MLASAAIVETVALALEELAPAVPLVLDPVMVAKGGAALLADSAAGALVKRLVPRARLLTPNIPEAEVLLGRAIKGADGME
GAARDLLALGPKAVLLKGGHLEGDHLVDVLAEAGGGLRRFTGRRIASRSTHGTGCTLASAIAAGLAQGLGLVEAVERARA
YLVRAIETAPGLGSGHGPLNHGHTVKEFQA
>Mature_269_residues
AMRGRVLIVAGSDSGGGAGIQADIKAVTCLGGFAATAVTALTAQNTLGVSGIHEVPPDFVRAQMAAVLSDIGADCIKTGM
LASAAIVETVALALEELAPAVPLVLDPVMVAKGGAALLADSAAGALVKRLVPRARLLTPNIPEAEVLLGRAIKGADGMEG
AARDLLALGPKAVLLKGGHLEGDHLVDVLAEAGGGLRRFTGRRIASRSTHGTGCTLASAIAAGLAQGLGLVEAVERARAY
LVRAIETAPGLGSGHGPLNHGHTVKEFQA

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=263, Percent_Identity=44.106463878327, Blast_Score=200, Evalue=9e-53,
Organism=Saccharomyces cerevisiae, GI6325378, Length=273, Percent_Identity=36.996336996337, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6324997, Length=274, Percent_Identity=38.3211678832117, Blast_Score=142, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6324517, Length=274, Percent_Identity=37.2262773722628, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 26996; Mature: 26865

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMRGRVLIVAGSDSGGGAGIQADIKAVTCLGGFAATAVTALTAQNTLGVSGIHEVPPDF
CCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCHHH
VRAQMAAVLSDIGADCIKTGMLASAAIVETVALALEELAPAVPLVLDPVMVAKGGAALLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHEEH
DSAAGALVKRLVPRARLLTPNIPEAEVLLGRAIKGADGMEGAARDLLALGPKAVLLKGGH
HHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEECCC
LEGDHLVDVLAEAGGGLRRFTGRRIASRSTHGTGCTLASAIAAGLAQGLGLVEAVERARA
CCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
YLVRAIETAPGLGSGHGPLNHGHTVKEFQA
HHHHHHHHCCCCCCCCCCCCCCCCHHHHCC
>Mature Secondary Structure 
AMRGRVLIVAGSDSGGGAGIQADIKAVTCLGGFAATAVTALTAQNTLGVSGIHEVPPDF
CCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCHHH
VRAQMAAVLSDIGADCIKTGMLASAAIVETVALALEELAPAVPLVLDPVMVAKGGAALLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHEEH
DSAAGALVKRLVPRARLLTPNIPEAEVLLGRAIKGADGMEGAARDLLALGPKAVLLKGGH
HHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEECCC
LEGDHLVDVLAEAGGGLRRFTGRRIASRSTHGTGCTLASAIAAGLAQGLGLVEAVERARA
CCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
YLVRAIETAPGLGSGHGPLNHGHTVKEFQA
HHHHHHHHCCCCCCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA