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The map label for this gene is glgX [H]

Identifier: 83312163

GI number: 83312163

Start: 3311463

End: 3313625

Strand: Reverse

Name: glgX [H]

Synonym: amb3064

Alternate gene names: 83312163

Gene position: 3313625-3311463 (Counterclockwise)

Preceding gene: 83312164

Following gene: 83312162

Centisome position: 66.71

GC content: 65.79

Gene sequence:

>2163_bases
ATGACCTCCCATCGCCGCATTCTTCCCGGCTCGGCCAATCCTCTTGGGGCGACCTGGGACGGCAATGGCGTGAACTTCGC
CCTGTTCTCGGCCCATGCCGAGAAGGTGGAGCTGTGCCTGTTCGACCGGCGCGGCCTGCGCGAGGTGGAGCGCATCGCCC
TGCCCGAATACACTGACGAGGTCTGGCACGGCTATCTGCCCGATTGCCGGCCGGGCCAGCTTTACGGCTATCGCGTCCAC
GGCCCCTACGACCCCAACGAGGGCCATCGCTTCAACGCCCACAAGCTGCTGCTCGATCCCTATGCCAAGGCTCATGCCGG
CAGCTTCGAATGGTCGGACACCCATTTCGGCTTCAAGCCGGGCACGCCGAAGCTGGACCTGGTGGCCGACAAGCGCGACA
ACGCCCGCTTCATGCCCAAGTGCCGGGTGATCGATACCGCCTTCACCTGGGGTGATGACCGCCGCCCCAACGTGCCGTGG
CCCGAGACCATCGTCTACGAGGCCCATGTGCGCGGCATGACCATGAAGCATCCCGGCGTGGTCAACGGCATGCGCGGCAC
CTTCCTGGGTCTGACCCAGCCCGGCGTGATCGACCACCTGCGGCGTCTGGGGGTGACCACGCTGCAATTGCTGCCCATCC
ACGCCATGATCGACGAGAAGCATCTGGCCGACCGGGGCATGCGCAATTACTGGGGCTACAATTCCATCGGCTTTTTCGCC
CCCGATGCCCGCTATTATTCCGCCAGCCCGCACGGCGACTTCAAGACCATGGTGTCGCGGCTGCACGACGTGGGCATCGA
GGTGATTCTGGACGTGGTCTACAACCACACCGCCGAGGGCAATCACCTGGGGCCGACGCTGTCCTATCGCGGCATCGACA
ATCTCAGCTATTACCGCCTGGGGCCGGACGGCAAGCGCTGGTACGAGAATTACTCGGGCTGCGGCAATACCTTGAACCTC
GCCCATCCCCGCGTGCTGCAGATGGTGATGGATTCCTTGCGCTATTGGGCCGAGGAGATGCACGTGGACGGCTTCCGCTT
CGATCTGGCGGCGTCGCTGGTGCGGGGCAAGGGCGGCTTCGACCATACCTCCGGCTTCCTCGACGCGGTGCGCCAGGACC
CGGTGCTGTCCCGGCTGAAGCTGATCGCCGAGCCTTGGGACTTAGGGGGAGACGGCTATCGGGTCGGGCGCTTTCCCCCC
GGCTGGTCCGAGTGGAACGGCCGCTACCGCGACACGGTGCGGCGCTTCTGGACGGGGGAGGGCGGGGTGATCGGCGATCT
GGCCTCGCGCCTGACCGGATCAAGCGACATCTTCGGCTGGGGCGGGCGGCGGCCCTGGGCCAGCTTGAACTTCGTCACCT
GCCACGACGGTTTCACCCTCAAGGACCTGGTGACCTACGAGCGCAAGCACAACGAGGCCAATCTCGAGGCCAACAAGGAC
GGCACCGACGCCAATTACGCCTGGAATTGCGGTCACGAGGGACCGTCGCCCTATCCCGAGATCACGGCGCTGAGGGCACG
GCAGAGCCGCAATCTGCTGGCCACCCTGCTGCTGTCCCAGGGCATTCCCATGCTGCTGGCCGGCGACGAGATGGGGCGGT
CGCAGAAGGGCAACAACAACGCCTATTGCCAGGACAATGAACTGGGCTGGGTCGCCTGGGATCAGGTGGACGAGGACCTG
CTGGCCTTCGTGCAGGGCCTGATCGCGCTGCGCAAGGCCCATCCGGTGTTCCGCCGTCCCCGCTTTTTCGAGGGCAAGCG
CCTGCCCAACTCGCCCCTGAAGGACATCGTCTGGGTGACGCCCGAGGGGCGCGAGATGGGCCATGCCGACTGGACCACGC
CCTTCGCCCGCTCGCTGGGCTTCGTGCTGGGGGGCGAATCCTGCGCCGTGGACAACCTCACGGGGCACGAAGAGATGGAC
GACACCTTCCTGGTGCTGCTCAACGCCTATCACGAGGCGGTACCCTATGTGCTGCCGCCGCCCAGCCTGGGGCGGTCGTG
GGAACTGGTGCTTGATACCAACGATCCCGCCGGCCGCCATGCGGGGACTCATTGGGCGGCGGGGATCACCTATCCGCTGG
GGCAGCGTTCCCTGGCGGTTTTGCGCCGTTCGGGCGGTATGCGCGGATTGCGGCGAGATGAAATCGAGGGGAATGAGGAA
TGA

Upstream 100 bases:

>100_bases
CTTCTCCATCTGCCTGCGGCTGCCGCCGTTGGCCACCTGCGTGTTCAAGCGGGAGCGGTAATGCGGTACAAGCGAAGGAC
AATTTCCGTTTGAGGCCCGC

Downstream 100 bases:

>100_bases
GCGGAACCGACGCTCTGGACCGTTTGGCGCGGCTGGCGGGAATCGAGGACGGCTGGTGGGACTTCTTCGGCGAATGGCGC
GTGGTGCCGCCTCAGACCAA

Product: pullulanase PulA and related glycosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 720; Mature: 719

Protein sequence:

>720_residues
MTSHRRILPGSANPLGATWDGNGVNFALFSAHAEKVELCLFDRRGLREVERIALPEYTDEVWHGYLPDCRPGQLYGYRVH
GPYDPNEGHRFNAHKLLLDPYAKAHAGSFEWSDTHFGFKPGTPKLDLVADKRDNARFMPKCRVIDTAFTWGDDRRPNVPW
PETIVYEAHVRGMTMKHPGVVNGMRGTFLGLTQPGVIDHLRRLGVTTLQLLPIHAMIDEKHLADRGMRNYWGYNSIGFFA
PDARYYSASPHGDFKTMVSRLHDVGIEVILDVVYNHTAEGNHLGPTLSYRGIDNLSYYRLGPDGKRWYENYSGCGNTLNL
AHPRVLQMVMDSLRYWAEEMHVDGFRFDLAASLVRGKGGFDHTSGFLDAVRQDPVLSRLKLIAEPWDLGGDGYRVGRFPP
GWSEWNGRYRDTVRRFWTGEGGVIGDLASRLTGSSDIFGWGGRRPWASLNFVTCHDGFTLKDLVTYERKHNEANLEANKD
GTDANYAWNCGHEGPSPYPEITALRARQSRNLLATLLLSQGIPMLLAGDEMGRSQKGNNNAYCQDNELGWVAWDQVDEDL
LAFVQGLIALRKAHPVFRRPRFFEGKRLPNSPLKDIVWVTPEGREMGHADWTTPFARSLGFVLGGESCAVDNLTGHEEMD
DTFLVLLNAYHEAVPYVLPPPSLGRSWELVLDTNDPAGRHAGTHWAAGITYPLGQRSLAVLRRSGGMRGLRRDEIEGNEE

Sequences:

>Translated_720_residues
MTSHRRILPGSANPLGATWDGNGVNFALFSAHAEKVELCLFDRRGLREVERIALPEYTDEVWHGYLPDCRPGQLYGYRVH
GPYDPNEGHRFNAHKLLLDPYAKAHAGSFEWSDTHFGFKPGTPKLDLVADKRDNARFMPKCRVIDTAFTWGDDRRPNVPW
PETIVYEAHVRGMTMKHPGVVNGMRGTFLGLTQPGVIDHLRRLGVTTLQLLPIHAMIDEKHLADRGMRNYWGYNSIGFFA
PDARYYSASPHGDFKTMVSRLHDVGIEVILDVVYNHTAEGNHLGPTLSYRGIDNLSYYRLGPDGKRWYENYSGCGNTLNL
AHPRVLQMVMDSLRYWAEEMHVDGFRFDLAASLVRGKGGFDHTSGFLDAVRQDPVLSRLKLIAEPWDLGGDGYRVGRFPP
GWSEWNGRYRDTVRRFWTGEGGVIGDLASRLTGSSDIFGWGGRRPWASLNFVTCHDGFTLKDLVTYERKHNEANLEANKD
GTDANYAWNCGHEGPSPYPEITALRARQSRNLLATLLLSQGIPMLLAGDEMGRSQKGNNNAYCQDNELGWVAWDQVDEDL
LAFVQGLIALRKAHPVFRRPRFFEGKRLPNSPLKDIVWVTPEGREMGHADWTTPFARSLGFVLGGESCAVDNLTGHEEMD
DTFLVLLNAYHEAVPYVLPPPSLGRSWELVLDTNDPAGRHAGTHWAAGITYPLGQRSLAVLRRSGGMRGLRRDEIEGNEE
>Mature_719_residues
TSHRRILPGSANPLGATWDGNGVNFALFSAHAEKVELCLFDRRGLREVERIALPEYTDEVWHGYLPDCRPGQLYGYRVHG
PYDPNEGHRFNAHKLLLDPYAKAHAGSFEWSDTHFGFKPGTPKLDLVADKRDNARFMPKCRVIDTAFTWGDDRRPNVPWP
ETIVYEAHVRGMTMKHPGVVNGMRGTFLGLTQPGVIDHLRRLGVTTLQLLPIHAMIDEKHLADRGMRNYWGYNSIGFFAP
DARYYSASPHGDFKTMVSRLHDVGIEVILDVVYNHTAEGNHLGPTLSYRGIDNLSYYRLGPDGKRWYENYSGCGNTLNLA
HPRVLQMVMDSLRYWAEEMHVDGFRFDLAASLVRGKGGFDHTSGFLDAVRQDPVLSRLKLIAEPWDLGGDGYRVGRFPPG
WSEWNGRYRDTVRRFWTGEGGVIGDLASRLTGSSDIFGWGGRRPWASLNFVTCHDGFTLKDLVTYERKHNEANLEANKDG
TDANYAWNCGHEGPSPYPEITALRARQSRNLLATLLLSQGIPMLLAGDEMGRSQKGNNNAYCQDNELGWVAWDQVDEDLL
AFVQGLIALRKAHPVFRRPRFFEGKRLPNSPLKDIVWVTPEGREMGHADWTTPFARSLGFVLGGESCAVDNLTGHEEMDD
TFLVLLNAYHEAVPYVLPPPSLGRSWELVLDTNDPAGRHAGTHWAAGITYPLGQRSLAVLRRSGGMRGLRRDEIEGNEE

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=654, Percent_Identity=46.3302752293578, Blast_Score=548, Evalue=1e-157,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 81008; Mature: 80877

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSHRRILPGSANPLGATWDGNGVNFALFSAHAEKVELCLFDRRGLREVERIALPEYTDE
CCCCCCCCCCCCCCCCCEECCCCCEEEEEECCCCEEEEEEECCCCHHHHHHHCCCCHHHH
VWHGYLPDCRPGQLYGYRVHGPYDPNEGHRFNAHKLLLDPYAKAHAGSFEWSDTHFGFKP
HHCCCCCCCCCCCEEEEEECCCCCCCCCCEECCEEEEECCHHHHCCCCCCCCCCCCCCCC
GTPKLDLVADKRDNARFMPKCRVIDTAFTWGDDRRPNVPWPETIVYEAHVRGMTMKHPGV
CCCCEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEEHHCCEEECCCCC
VNGMRGTFLGLTQPGVIDHLRRLGVTTLQLLPIHAMIDEKHLADRGMRNYWGYNSIGFFA
CCCCCCCEECCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCHHHHCCCCCCCEEC
PDARYYSASPHGDFKTMVSRLHDVGIEVILDVVYNHTAEGNHLGPTLSYRGIDNLSYYRL
CCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCEEEEE
GPDGKRWYENYSGCGNTLNLAHPRVLQMVMDSLRYWAEEMHVDGFRFDLAASLVRGKGGF
CCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
DHTSGFLDAVRQDPVLSRLKLIAEPWDLGGDGYRVGRFPPGWSEWNGRYRDTVRRFWTGE
CHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHCCCHHHHHHHHHHCCC
GGVIGDLASRLTGSSDIFGWGGRRPWASLNFVTCHDGFTLKDLVTYERKHNEANLEANKD
CCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCC
GTDANYAWNCGHEGPSPYPEITALRARQSRNLLATLLLSQGIPMLLAGDEMGRSQKGNNN
CCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHCCCCCCCCC
AYCQDNELGWVAWDQVDEDLLAFVQGLIALRKAHPVFRRPRFFEGKRLPNSPLKDIVWVT
CCEECCCCCEEEHHHCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCEEEEC
PEGREMGHADWTTPFARSLGFVLGGESCAVDNLTGHEEMDDTFLVLLNAYHEAVPYVLPP
CCCCCCCCCCCCCHHHHHCCCEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCEECC
PSLGRSWELVLDTNDPAGRHAGTHWAAGITYPLGQRSLAVLRRSGGMRGLRRDEIEGNEE
CCCCCCEEEEEECCCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSHRRILPGSANPLGATWDGNGVNFALFSAHAEKVELCLFDRRGLREVERIALPEYTDE
CCCCCCCCCCCCCCCCEECCCCCEEEEEECCCCEEEEEEECCCCHHHHHHHCCCCHHHH
VWHGYLPDCRPGQLYGYRVHGPYDPNEGHRFNAHKLLLDPYAKAHAGSFEWSDTHFGFKP
HHCCCCCCCCCCCEEEEEECCCCCCCCCCEECCEEEEECCHHHHCCCCCCCCCCCCCCCC
GTPKLDLVADKRDNARFMPKCRVIDTAFTWGDDRRPNVPWPETIVYEAHVRGMTMKHPGV
CCCCEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEEHHCCEEECCCCC
VNGMRGTFLGLTQPGVIDHLRRLGVTTLQLLPIHAMIDEKHLADRGMRNYWGYNSIGFFA
CCCCCCCEECCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCHHHHCCCCCCCEEC
PDARYYSASPHGDFKTMVSRLHDVGIEVILDVVYNHTAEGNHLGPTLSYRGIDNLSYYRL
CCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCEEEEE
GPDGKRWYENYSGCGNTLNLAHPRVLQMVMDSLRYWAEEMHVDGFRFDLAASLVRGKGGF
CCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
DHTSGFLDAVRQDPVLSRLKLIAEPWDLGGDGYRVGRFPPGWSEWNGRYRDTVRRFWTGE
CHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHCCCHHHHHHHHHHCCC
GGVIGDLASRLTGSSDIFGWGGRRPWASLNFVTCHDGFTLKDLVTYERKHNEANLEANKD
CCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCC
GTDANYAWNCGHEGPSPYPEITALRARQSRNLLATLLLSQGIPMLLAGDEMGRSQKGNNN
CCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHCCCCCCCCC
AYCQDNELGWVAWDQVDEDLLAFVQGLIALRKAHPVFRRPRFFEGKRLPNSPLKDIVWVT
CCEECCCCCEEEHHHCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCEEEEC
PEGREMGHADWTTPFARSLGFVLGGESCAVDNLTGHEEMDDTFLVLLNAYHEAVPYVLPP
CCCCCCCCCCCCCHHHHHCCCEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCEECC
PSLGRSWELVLDTNDPAGRHAGTHWAAGITYPLGQRSLAVLRRSGGMRGLRRDEIEGNEE
CCCCCCEEEEEECCCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]