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The map label for this gene is glgB

Identifier: 83312164

GI number: 83312164

Start: 3313665

End: 3315887

Strand: Reverse

Name: glgB

Synonym: amb3065

Alternate gene names: 83312164

Gene position: 3315887-3313665 (Counterclockwise)

Preceding gene: 83312166

Following gene: 83312163

Centisome position: 66.76

GC content: 65.36

Gene sequence:

>2223_bases
TTGGGATCTTCGACTGGCAGGAGGGGGCTGATGCCCGAAGCGGCGAAATTGATCCGGTCCAGCGATGCCGATGCCATCGC
CCGGGGACGTCATGGCGATCCCTTCGCCGTGCTGGGTCCCCATGCGGTCAAGGGCGGCACGGTGATCCGTACCTTCCAGC
CCCAGGCCCGAACGGTCTTCGTGCTGGCCGCCGATGGCGAGACCGAGATGAGCCGGGCTCACCCCGACGGCCTGTTCGCT
GTCAAGCTGAAGGGTTCGCCCGCCTACCGGCTGCGGGCGGTGCGCCATGACGGCGGGACCGAAGAACTGGACGACCCCTA
TCGCTTCCCCCCGGTGCTGGGCGATCTCGACGTCCACCTGCTGGCCGAGGGGACCCACCTGCGGACCTTCGAGAAGCTCG
GCGCCCAGGTTCGGATTGTCGACGGGGTGGCCGGGGTCGACTTCGCCCTGTGGGCGCCCAATGCCTCCCGCGTTTCGGTG
GTGGGCGACTTTAACGGCTGGGACGGGCGGCGCCATCCTATGCGCCTGCGCCATGAGGCGGGAATGTGGGAAATCTTCAT
TCCCGGCCTGGGGCAGGGGGCCGTCTACAAATATGAAATCGTCGCCTCCGACGGGCGGCTGCTGCCCCTGAAGGCCGACC
CTTACGGCCATTTCGCCGAGGTGCCGCCCAAGACCGCCTCGGTGGTGTGGGAACTGGGCCGCCGCGACTGGGCCGATGCC
GACTGGATGGCGGCGCAGAAGGCGCGCAATGACCGCCATGCCCCCATCAGCATCTATGAGGTGCATCTGGGGTCGTGGCG
GCGCGTGCCGGAAGATTGCAACCGGCCGCTGTCCTATCTCGAAATGGCCGACCAGCTGGGCGATTACGTGGCCGACCTGG
GCTTCACCCATGTGGAGTTCCTGCCCATTCACGAGCATCCCTTCGGCGGCTCGTGGGGCTATCAGCCGGTGGGGCTGTTC
GCGCCCACCTCGCGCTACGGCACGCCGGACGAGTTCCGCACCCTGGTCGATCGCCTGCACCAAAAGGGCATCGGCGTGAT
CATCGACTGGGTGGCCGGGCATTTCCCCAACGATCCCCACGGCCTGCACCATTTCGACGGCACCCATCTCTACGAGCACG
AGGACCCGCGCCTGGGCGTGCACAAGGACTGGAACACCCTGATCTACAATTACGGCCGCAGCGAAGTGGTGAACTATCTC
TACGCCAACGCCCTGTACTGGCTGGAGCAGTATCACGTGGACGGCCTGCGCGTGGATGCGGTGGCGTCCATGCTGTACCT
GGATTACTCGCGCGAGCCGGGCGAGTGGATTCCCAACCGCCACGGCGGTAACGAGAATCTGGAAGCCATCGACTTCCTGC
GCCGCATGAACATGCTGGTCTATGCCGAGCATCCGGGCGCCATGACCATTGCCGAGGAATCCACCGCCTGGCCCATGGTC
TCGCGGCCGGTGCATCTGGGCGGGCTGGGCTTCGGCTACAAGTGGAACATGGGGTGGATGCACGACACGCTGCGCTACTT
CTCCAAGGACCCCATCCACCGCCGCTATCACCATGACAGCCTGACCTTCGCCCAGCTCTATGCCTATCACGAGAATTTCG
TGCTGCCGCTGTCCCACGACGAGGTGGTGCACGGCAAGGGTTCGATCTTCGGCCGCATGCCGGGCGATCCCTGGCAGCGC
TTTGCCAATCTGCGCGCCTATTACGGCTTCATGTGGACCCAGCCGGGCAAGAAGCTGCTGTTCATGGGCCAGGAATTCGC
CCAGCAAAGCGAGTGGAACGAGGATGCCAGCCTGGACTGGCACCTGCTGGGCGACGGGCGCAACGAAGGCGTCATGCGCC
TGATCCGCGACCTGAACCGCCTGTACCGCACCGAGCCAGCGCTGCACCAGTTGGACAATGAGCCGGCGGGCTTTGCCTGG
ATCGACTGCAACGACCGCGACAATTCGGTTCTGACCTGGCTGCGCAAGGGCTTCGATCCCGGCGACTTCCTGGTGGTGGC
GGGCAATTACACTCCCATGGTGCGCGACTCCTATCGCATCGGCGTGCCCGAGCCTGGATGGTACCGCGAGCTGCTCAACA
CCGATTCCGAATGGTATGGCGGCGCCAACATCCATAACGGCGGCGGAGTTCACACCGAGGAGGTGCCCTGGCACGGCCAC
GGCTTCTCCATCTGCCTGCGGCTGCCGCCGTTGGCCACCTGCGTGTTCAAGCGGGAGCGGTAA

Upstream 100 bases:

>100_bases
GGGCTTTGCGGAAGGTCCAATCAATCCAATTAAAAAGCAGCCCCCGTAAAACGTCCGTTGTGAAATCGCCCTTTGTCTTT
CCGGCCAAAGGCGGTGACAC

Downstream 100 bases:

>100_bases
TGCGGTACAAGCGAAGGACAATTTCCGTTTGAGGCCCGCATGACCTCCCATCGCCGCATTCTTCCCGGCTCGGCCAATCC
TCTTGGGGCGACCTGGGACG

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 740; Mature: 739

Protein sequence:

>740_residues
MGSSTGRRGLMPEAAKLIRSSDADAIARGRHGDPFAVLGPHAVKGGTVIRTFQPQARTVFVLAADGETEMSRAHPDGLFA
VKLKGSPAYRLRAVRHDGGTEELDDPYRFPPVLGDLDVHLLAEGTHLRTFEKLGAQVRIVDGVAGVDFALWAPNASRVSV
VGDFNGWDGRRHPMRLRHEAGMWEIFIPGLGQGAVYKYEIVASDGRLLPLKADPYGHFAEVPPKTASVVWELGRRDWADA
DWMAAQKARNDRHAPISIYEVHLGSWRRVPEDCNRPLSYLEMADQLGDYVADLGFTHVEFLPIHEHPFGGSWGYQPVGLF
APTSRYGTPDEFRTLVDRLHQKGIGVIIDWVAGHFPNDPHGLHHFDGTHLYEHEDPRLGVHKDWNTLIYNYGRSEVVNYL
YANALYWLEQYHVDGLRVDAVASMLYLDYSREPGEWIPNRHGGNENLEAIDFLRRMNMLVYAEHPGAMTIAEESTAWPMV
SRPVHLGGLGFGYKWNMGWMHDTLRYFSKDPIHRRYHHDSLTFAQLYAYHENFVLPLSHDEVVHGKGSIFGRMPGDPWQR
FANLRAYYGFMWTQPGKKLLFMGQEFAQQSEWNEDASLDWHLLGDGRNEGVMRLIRDLNRLYRTEPALHQLDNEPAGFAW
IDCNDRDNSVLTWLRKGFDPGDFLVVAGNYTPMVRDSYRIGVPEPGWYRELLNTDSEWYGGANIHNGGGVHTEEVPWHGH
GFSICLRLPPLATCVFKRER

Sequences:

>Translated_740_residues
MGSSTGRRGLMPEAAKLIRSSDADAIARGRHGDPFAVLGPHAVKGGTVIRTFQPQARTVFVLAADGETEMSRAHPDGLFA
VKLKGSPAYRLRAVRHDGGTEELDDPYRFPPVLGDLDVHLLAEGTHLRTFEKLGAQVRIVDGVAGVDFALWAPNASRVSV
VGDFNGWDGRRHPMRLRHEAGMWEIFIPGLGQGAVYKYEIVASDGRLLPLKADPYGHFAEVPPKTASVVWELGRRDWADA
DWMAAQKARNDRHAPISIYEVHLGSWRRVPEDCNRPLSYLEMADQLGDYVADLGFTHVEFLPIHEHPFGGSWGYQPVGLF
APTSRYGTPDEFRTLVDRLHQKGIGVIIDWVAGHFPNDPHGLHHFDGTHLYEHEDPRLGVHKDWNTLIYNYGRSEVVNYL
YANALYWLEQYHVDGLRVDAVASMLYLDYSREPGEWIPNRHGGNENLEAIDFLRRMNMLVYAEHPGAMTIAEESTAWPMV
SRPVHLGGLGFGYKWNMGWMHDTLRYFSKDPIHRRYHHDSLTFAQLYAYHENFVLPLSHDEVVHGKGSIFGRMPGDPWQR
FANLRAYYGFMWTQPGKKLLFMGQEFAQQSEWNEDASLDWHLLGDGRNEGVMRLIRDLNRLYRTEPALHQLDNEPAGFAW
IDCNDRDNSVLTWLRKGFDPGDFLVVAGNYTPMVRDSYRIGVPEPGWYRELLNTDSEWYGGANIHNGGGVHTEEVPWHGH
GFSICLRLPPLATCVFKRER
>Mature_739_residues
GSSTGRRGLMPEAAKLIRSSDADAIARGRHGDPFAVLGPHAVKGGTVIRTFQPQARTVFVLAADGETEMSRAHPDGLFAV
KLKGSPAYRLRAVRHDGGTEELDDPYRFPPVLGDLDVHLLAEGTHLRTFEKLGAQVRIVDGVAGVDFALWAPNASRVSVV
GDFNGWDGRRHPMRLRHEAGMWEIFIPGLGQGAVYKYEIVASDGRLLPLKADPYGHFAEVPPKTASVVWELGRRDWADAD
WMAAQKARNDRHAPISIYEVHLGSWRRVPEDCNRPLSYLEMADQLGDYVADLGFTHVEFLPIHEHPFGGSWGYQPVGLFA
PTSRYGTPDEFRTLVDRLHQKGIGVIIDWVAGHFPNDPHGLHHFDGTHLYEHEDPRLGVHKDWNTLIYNYGRSEVVNYLY
ANALYWLEQYHVDGLRVDAVASMLYLDYSREPGEWIPNRHGGNENLEAIDFLRRMNMLVYAEHPGAMTIAEESTAWPMVS
RPVHLGGLGFGYKWNMGWMHDTLRYFSKDPIHRRYHHDSLTFAQLYAYHENFVLPLSHDEVVHGKGSIFGRMPGDPWQRF
ANLRAYYGFMWTQPGKKLLFMGQEFAQQSEWNEDASLDWHLLGDGRNEGVMRLIRDLNRLYRTEPALHQLDNEPAGFAWI
DCNDRDNSVLTWLRKGFDPGDFLVVAGNYTPMVRDSYRIGVPEPGWYRELLNTDSEWYGGANIHNGGGVHTEEVPWHGHG
FSICLRLPPLATCVFKRER

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=676, Percent_Identity=27.0710059171598, Blast_Score=188, Evalue=2e-47,
Organism=Escherichia coli, GI1789839, Length=725, Percent_Identity=56.4137931034483, Blast_Score=788, Evalue=0.0,
Organism=Escherichia coli, GI2367229, Length=340, Percent_Identity=28.5294117647059, Blast_Score=67, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17554896, Length=665, Percent_Identity=26.3157894736842, Blast_Score=192, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI32564391, Length=629, Percent_Identity=25.9141494435612, Blast_Score=166, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6320826, Length=377, Percent_Identity=30.2387267904509, Blast_Score=158, Evalue=3e-39,
Organism=Drosophila melanogaster, GI28573410, Length=647, Percent_Identity=26.7387944358578, Blast_Score=181, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_MAGSA (Q2W2Q6)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_422428.1
- ProteinModelPortal:   Q2W2Q6
- STRING:   Q2W2Q6
- GeneID:   3804549
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb3065
- NMPDR:   fig|342108.5.peg.2746
- eggNOG:   COG0296
- HOGENOM:   HBG287139
- OMA:   DGTCLYE
- PhylomeDB:   Q2W2Q6
- ProtClustDB:   PRK05402
- BioCyc:   MMAG342108:AMB3065-MONOMER
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: =2.4.1.18

Molecular weight: Translated: 84013; Mature: 83881

Theoretical pI: Translated: 6.49; Mature: 6.49

Prosite motif: NA

Important sites: ACT_SITE 314-314 ACT_SITE 349-349 ACT_SITE 354-354 ACT_SITE 417-417 ACT_SITE 419-419 ACT_SITE 472-472 ACT_SITE 539-539 ACT_SITE 540-540

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSSTGRRGLMPEAAKLIRSSDADAIARGRHGDPFAVLGPHAVKGGTVIRTFQPQARTVF
CCCCCCCCCCCHHHHHHHHCCCCHHHHCCCCCCCEEEECCCCCCCCEEEEEECCCCEEEE
VLAADGETEMSRAHPDGLFAVKLKGSPAYRLRAVRHDGGTEELDDPYRFPPVLGDLDVHL
EEEECCCHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEE
LAEGTHLRTFEKLGAQVRIVDGVAGVDFALWAPNASRVSVVGDFNGWDGRRHPMRLRHEA
EECCCCHHHHHHCCCEEEEEECCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCHHHHCC
GMWEIFIPGLGQGAVYKYEIVASDGRLLPLKADPYGHFAEVPPKTASVVWELGRRDWADA
CEEEEEECCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCH
DWMAAQKARNDRHAPISIYEVHLGSWRRVPEDCNRPLSYLEMADQLGDYVADLGFTHVEF
HHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCEEEE
LPIHEHPFGGSWGYQPVGLFAPTSRYGTPDEFRTLVDRLHQKGIGVIIDWVAGHFPNDPH
EEECCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHCCCCCCCC
GLHHFDGTHLYEHEDPRLGVHKDWNTLIYNYGRSEVVNYLYANALYWLEQYHVDGLRVDA
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEHHH
VASMLYLDYSREPGEWIPNRHGGNENLEAIDFLRRMNMLVYAEHPGAMTIAEESTAWPMV
HHHHHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEECCCEEEEECCCCCCCCC
SRPVHLGGLGFGYKWNMGWMHDTLRYFSKDPIHRRYHHDSLTFAQLYAYHENFVLPLSHD
CCCEEECCCCCCEEECCCHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHCCEEEECCCC
EVVHGKGSIFGRMPGDPWQRFANLRAYYGFMWTQPGKKLLFMGQEFAQQSEWNEDASLDW
CEEECCCCEEECCCCCHHHHHHHHHHHEEEEEECCCCEEEEECHHHHHHCCCCCCCCEEE
HLLGDGRNEGVMRLIRDLNRLYRTEPALHQLDNEPAGFAWIDCNDRDNSVLTWLRKGFDP
EEECCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCC
GDFLVVAGNYTPMVRDSYRIGVPEPGWYRELLNTDSEWYGGANIHNGGGVHTEEVPWHGH
CCEEEEECCCCCCCCCCEEECCCCCHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCC
GFSICLRLPPLATCVFKRER
CEEEEEECCCHHHHHEECCC
>Mature Secondary Structure 
GSSTGRRGLMPEAAKLIRSSDADAIARGRHGDPFAVLGPHAVKGGTVIRTFQPQARTVF
CCCCCCCCCCHHHHHHHHCCCCHHHHCCCCCCCEEEECCCCCCCCEEEEEECCCCEEEE
VLAADGETEMSRAHPDGLFAVKLKGSPAYRLRAVRHDGGTEELDDPYRFPPVLGDLDVHL
EEEECCCHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEE
LAEGTHLRTFEKLGAQVRIVDGVAGVDFALWAPNASRVSVVGDFNGWDGRRHPMRLRHEA
EECCCCHHHHHHCCCEEEEEECCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCHHHHCC
GMWEIFIPGLGQGAVYKYEIVASDGRLLPLKADPYGHFAEVPPKTASVVWELGRRDWADA
CEEEEEECCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCH
DWMAAQKARNDRHAPISIYEVHLGSWRRVPEDCNRPLSYLEMADQLGDYVADLGFTHVEF
HHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCCEEEE
LPIHEHPFGGSWGYQPVGLFAPTSRYGTPDEFRTLVDRLHQKGIGVIIDWVAGHFPNDPH
EEECCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHCCCCCCCC
GLHHFDGTHLYEHEDPRLGVHKDWNTLIYNYGRSEVVNYLYANALYWLEQYHVDGLRVDA
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEHHH
VASMLYLDYSREPGEWIPNRHGGNENLEAIDFLRRMNMLVYAEHPGAMTIAEESTAWPMV
HHHHHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEECCCEEEEECCCCCCCCC
SRPVHLGGLGFGYKWNMGWMHDTLRYFSKDPIHRRYHHDSLTFAQLYAYHENFVLPLSHD
CCCEEECCCCCCEEECCCHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHCCEEEECCCC
EVVHGKGSIFGRMPGDPWQRFANLRAYYGFMWTQPGKKLLFMGQEFAQQSEWNEDASLDW
CEEECCCCEEECCCCCHHHHHHHHHHHEEEEEECCCCEEEEECHHHHHHCCCCCCCCEEE
HLLGDGRNEGVMRLIRDLNRLYRTEPALHQLDNEPAGFAWIDCNDRDNSVLTWLRKGFDP
EEECCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCC
GDFLVVAGNYTPMVRDSYRIGVPEPGWYRELLNTDSEWYGGANIHNGGGVHTEEVPWHGH
CCEEEEECCCCCCCCCCEEECCCCCHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCC
GFSICLRLPPLATCVFKRER
CEEEEEECCCHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA