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The map label for this gene is braD [H]

Identifier: 83311972

GI number: 83311972

Start: 3072756

End: 3073616

Strand: Reverse

Name: braD [H]

Synonym: amb2873

Alternate gene names: 83311972

Gene position: 3073616-3072756 (Counterclockwise)

Preceding gene: 83311973

Following gene: 83311971

Centisome position: 61.88

GC content: 64.92

Gene sequence:

>861_bases
ATGGATTTCTGGTTTTTCCTGATTCAGGTGCTGAACGGCGTCCAATACGGGCTGCTGCTGTTCCTGGTCGCCTCCGGGCT
GACCCTGATCTTCGGCATCATGGGCATCATCAATCTGGCCCATGGCGCCTTCTACATGCTGGGCGCCTATCTGGCCTATT
GGCTGGCCCGGACCACCGGCAGCCTCGGTGCGGCGGTGCTGCTGTCGCTGCCCATCGCCGCCGCCATCGGCTATGGCATC
GAGGCCCTGCTGGTTCGCACCCTGTATCGCCGCGACCATCTGGATCAGGTGCTGCTGACCTATGGTCTGATCCTGATCTT
CAACGAGGCCACCCGCATGATCTGGGGGGCCGATGTGCACGGCGTGGCGGTGCCCGCCGCGCTCAACTGGTCCATCCGGC
TGACCGACACTCTGTCCTATCCCGTCTACCGCCTGATGCTGTCGGGGGTCTGCGCCGTGCTGGCCGTCGGCATGTATTTG
GTCATTACCCGGACCCGCTTCGGCATGTGGGTGCGGGCCGGGGCCTCCAATCGCGACATGGTGGCGGCGCTGGGCATCAA
CGTGAAGCTGCTGTTCGGCGTGGTGTTCGCCCTGGGCGCCGCCCTGGCCGGACTGGCCGGAGCCATCTCGACGCCCATCA
CCTCGGTGGCGCCGGGCATGGGGGATTCCGTGTTGATCCTGTGCTTCGTGGTGGTGGTGATCGGCGGCGTCGGCTCCATC
AAGGGCGCCTTTCTCGGCGCCATGCTGATCGGTCTGGCCGACACCTTCGGCAAGGTCTTCGCCCCCGATTTCGCCAGTTT
CACCGTCTATGGCGTGATGGCGGCGGTGCTATTGTGGAAGCCCCGGGGGCTGTTCTCATGA

Upstream 100 bases:

>100_bases
CGCGGAGGTGGAGGCCGCTGCCCGCCGGACCCTTGGTGCCGGTGGGGGCGGGCTTCGCGGGGCGGTGAACTCGACTCGAC
GCGCGCGGTCTGGAGCCACG

Downstream 100 bases:

>100_bases
ACCGTCTTCCCCGCCGGCTTTCCCTGCTTCTCCTGGTGCTGCTGGCGGCCCTGGCGCTGTTCCCGGCCTGGGGGCCGCTG
GCCTTTTCCGCCAAATACGC

Product: branched chain amino acid ABC transporter

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MDFWFFLIQVLNGVQYGLLLFLVASGLTLIFGIMGIINLAHGAFYMLGAYLAYWLARTTGSLGAAVLLSLPIAAAIGYGI
EALLVRTLYRRDHLDQVLLTYGLILIFNEATRMIWGADVHGVAVPAALNWSIRLTDTLSYPVYRLMLSGVCAVLAVGMYL
VITRTRFGMWVRAGASNRDMVAALGINVKLLFGVVFALGAALAGLAGAISTPITSVAPGMGDSVLILCFVVVVIGGVGSI
KGAFLGAMLIGLADTFGKVFAPDFASFTVYGVMAAVLLWKPRGLFS

Sequences:

>Translated_286_residues
MDFWFFLIQVLNGVQYGLLLFLVASGLTLIFGIMGIINLAHGAFYMLGAYLAYWLARTTGSLGAAVLLSLPIAAAIGYGI
EALLVRTLYRRDHLDQVLLTYGLILIFNEATRMIWGADVHGVAVPAALNWSIRLTDTLSYPVYRLMLSGVCAVLAVGMYL
VITRTRFGMWVRAGASNRDMVAALGINVKLLFGVVFALGAALAGLAGAISTPITSVAPGMGDSVLILCFVVVVIGGVGSI
KGAFLGAMLIGLADTFGKVFAPDFASFTVYGVMAAVLLWKPRGLFS
>Mature_286_residues
MDFWFFLIQVLNGVQYGLLLFLVASGLTLIFGIMGIINLAHGAFYMLGAYLAYWLARTTGSLGAAVLLSLPIAAAIGYGI
EALLVRTLYRRDHLDQVLLTYGLILIFNEATRMIWGADVHGVAVPAALNWSIRLTDTLSYPVYRLMLSGVCAVLAVGMYL
VITRTRFGMWVRAGASNRDMVAALGINVKLLFGVVFALGAALAGLAGAISTPITSVAPGMGDSVLILCFVVVVIGGVGSI
KGAFLGAMLIGLADTFGKVFAPDFASFTVYGVMAAVLLWKPRGLFS

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=311, Percent_Identity=26.6881028938907, Blast_Score=94, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 30370; Mature: 30370

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFWFFLIQVLNGVQYGLLLFLVASGLTLIFGIMGIINLAHGAFYMLGAYLAYWLARTTG
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SLGAAVLLSLPIAAAIGYGIEALLVRTLYRRDHLDQVLLTYGLILIFNEATRMIWGADVH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GVAVPAALNWSIRLTDTLSYPVYRLMLSGVCAVLAVGMYLVITRTRFGMWVRAGASNRDM
CEEECEEECCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCE
VAALGINVKLLFGVVFALGAALAGLAGAISTPITSVAPGMGDSVLILCFVVVVIGGVGSI
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHCCCCHH
KGAFLGAMLIGLADTFGKVFAPDFASFTVYGVMAAVLLWKPRGLFS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDFWFFLIQVLNGVQYGLLLFLVASGLTLIFGIMGIINLAHGAFYMLGAYLAYWLARTTG
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SLGAAVLLSLPIAAAIGYGIEALLVRTLYRRDHLDQVLLTYGLILIFNEATRMIWGADVH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GVAVPAALNWSIRLTDTLSYPVYRLMLSGVCAVLAVGMYLVITRTRFGMWVRAGASNRDM
CEEECEEECCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCE
VAALGINVKLLFGVVFALGAALAGLAGAISTPITSVAPGMGDSVLILCFVVVVIGGVGSI
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHCCCCHH
KGAFLGAMLIGLADTFGKVFAPDFASFTVYGVMAAVLLWKPRGLFS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]