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The map label for this gene is livM [H]

Identifier: 83311971

GI number: 83311971

Start: 3071770

End: 3072759

Strand: Reverse

Name: livM [H]

Synonym: amb2872

Alternate gene names: 83311971

Gene position: 3072759-3071770 (Counterclockwise)

Preceding gene: 83311972

Following gene: 83311970

Centisome position: 61.86

GC content: 66.57

Gene sequence:

>990_bases
ATGAACCGTCTTCCCCGCCGGCTTTCCCTGCTTCTCCTGGTGCTGCTGGCGGCCCTGGCGCTGTTCCCGGCCTGGGGGCC
GCTGGCCTTTTCCGCCAAATACGCCTTCCTGCTGCAAAAGCTGACCGGCATCATGATCCTGGCCATCCTGGCCATGAGCC
TCGACCTGCTGGTGGGCGTGGCCGGGCTGGTCAGCCTGGGGCACGCGGCGTTCTTCGGCCTGGGCGGCTACATGCTGGCC
CTGCTGAGCCCGCAATACGAGGCGGCCAATGCCTGGGTGGTGCTGCCCGCCGCCATGGGGGCGGTGGCGGCGGTCTCGGC
GGTGGTGGGCTTTCTGGCCATCCGCACCGCCGGCATCTACTTCATCATGGTCACCCTGGCCTTCGGGCAGATGGGCTATT
ATTTCTTCAATGATTCCAAGCTGGCCGGCGGCTCGGACGGGGCCTACATCTACGTCAAGCCGAATGTGGAGGCCTTCGGG
ATCACCCTGGTCAACCTGGAGAGCAAGCAGGCCTTCTTCTTCGTGGTCCTGGGCTGCCTGGTGGCGGTTTATCTGTGGCT
TCGCGTGGTGCTGGCCTCGCCCTTCGGCCGGGTGCTGGCCGCCATCGGCGTCAACGAGGGGCGGGTGCGCGGCCTGGGCT
TCAATCCCATGGTCTACAAGCTGGTGGCCTTCGCCCTGGCAGGAGCCCTGGCCGGTCTGGCGGGCTTTCTGGCGGCCACC
CAGTACGGCTTCGTCAGCCCCGCCATGCTGGGCTGGCATCAATCGGGCCATGTGCTGGTGATGGTCATCCTGGGCGGCAT
GGGCACCCTGTTCGGCCCGGTGCTGGGGGCCTTCATCCTGGAACTGGCCCATTTCGGCCTGGAAGCGGTGACCGAGCATT
GGCTGCTGCCCATGGGCGTGCTGGTGATCACCATCGTGCTCGCCCTGCCCAAGGGCGTGGCGGGGTTGTTGCTGCAATGG
TGCGGCAAGAAAGGGGAGGGGGAGCCATGA

Upstream 100 bases:

>100_bases
CCGACACCTTCGGCAAGGTCTTCGCCCCCGATTTCGCCAGTTTCACCGTCTATGGCGTGATGGCGGCGGTGCTATTGTGG
AAGCCCCGGGGGCTGTTCTC

Downstream 100 bases:

>100_bases
GCGTCTCTCCCTTTCTGTTCGAGACCAAGGGCCTGACCAAGGCCTTCGGCGGCCTGATCGCGGTGAATTCGGTGTCCATC
GGTTTTCGGGAGCGCGAGGC

Product: ABC-type branched-chain amino acid transport system

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MNRLPRRLSLLLLVLLAALALFPAWGPLAFSAKYAFLLQKLTGIMILAILAMSLDLLVGVAGLVSLGHAAFFGLGGYMLA
LLSPQYEAANAWVVLPAAMGAVAAVSAVVGFLAIRTAGIYFIMVTLAFGQMGYYFFNDSKLAGGSDGAYIYVKPNVEAFG
ITLVNLESKQAFFFVVLGCLVAVYLWLRVVLASPFGRVLAAIGVNEGRVRGLGFNPMVYKLVAFALAGALAGLAGFLAAT
QYGFVSPAMLGWHQSGHVLVMVILGGMGTLFGPVLGAFILELAHFGLEAVTEHWLLPMGVLVITIVLALPKGVAGLLLQW
CGKKGEGEP

Sequences:

>Translated_329_residues
MNRLPRRLSLLLLVLLAALALFPAWGPLAFSAKYAFLLQKLTGIMILAILAMSLDLLVGVAGLVSLGHAAFFGLGGYMLA
LLSPQYEAANAWVVLPAAMGAVAAVSAVVGFLAIRTAGIYFIMVTLAFGQMGYYFFNDSKLAGGSDGAYIYVKPNVEAFG
ITLVNLESKQAFFFVVLGCLVAVYLWLRVVLASPFGRVLAAIGVNEGRVRGLGFNPMVYKLVAFALAGALAGLAGFLAAT
QYGFVSPAMLGWHQSGHVLVMVILGGMGTLFGPVLGAFILELAHFGLEAVTEHWLLPMGVLVITIVLALPKGVAGLLLQW
CGKKGEGEP
>Mature_329_residues
MNRLPRRLSLLLLVLLAALALFPAWGPLAFSAKYAFLLQKLTGIMILAILAMSLDLLVGVAGLVSLGHAAFFGLGGYMLA
LLSPQYEAANAWVVLPAAMGAVAAVSAVVGFLAIRTAGIYFIMVTLAFGQMGYYFFNDSKLAGGSDGAYIYVKPNVEAFG
ITLVNLESKQAFFFVVLGCLVAVYLWLRVVLASPFGRVLAAIGVNEGRVRGLGFNPMVYKLVAFALAGALAGLAGFLAAT
QYGFVSPAMLGWHQSGHVLVMVILGGMGTLFGPVLGAFILELAHFGLEAVTEHWLLPMGVLVITIVLALPKGVAGLLLQW
CGKKGEGEP

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=291, Percent_Identity=30.9278350515464, Blast_Score=118, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 34654; Mature: 34654

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS00358 RIBOSOMAL_L5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRLPRRLSLLLLVLLAALALFPAWGPLAFSAKYAFLLQKLTGIMILAILAMSLDLLVGV
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLVSLGHAAFFGLGGYMLALLSPQYEAANAWVVLPAAMGAVAAVSAVVGFLAIRTAGIY
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
FIMVTLAFGQMGYYFFNDSKLAGGSDGAYIYVKPNVEAFGITLVNLESKQAFFFVVLGCL
HHHHHHHHHHCCEEEECCCEECCCCCCEEEEEECCCCEEEEEEEEECCCHHHHHHHHHHH
VAVYLWLRVVLASPFGRVLAAIGVNEGRVRGLGFNPMVYKLVAFALAGALAGLAGFLAAT
HHHHHHHHHHHHCHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QYGFVSPAMLGWHQSGHVLVMVILGGMGTLFGPVLGAFILELAHFGLEAVTEHWLLPMGV
HCCCCCHHHHCCCCCCCEEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVITIVLALPKGVAGLLLQWCGKKGEGEP
HHHHHHHHHCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNRLPRRLSLLLLVLLAALALFPAWGPLAFSAKYAFLLQKLTGIMILAILAMSLDLLVGV
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLVSLGHAAFFGLGGYMLALLSPQYEAANAWVVLPAAMGAVAAVSAVVGFLAIRTAGIY
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
FIMVTLAFGQMGYYFFNDSKLAGGSDGAYIYVKPNVEAFGITLVNLESKQAFFFVVLGCL
HHHHHHHHHHCCEEEECCCEECCCCCCEEEEEECCCCEEEEEEEEECCCHHHHHHHHHHH
VAVYLWLRVVLASPFGRVLAAIGVNEGRVRGLGFNPMVYKLVAFALAGALAGLAGFLAAT
HHHHHHHHHHHHCHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QYGFVSPAMLGWHQSGHVLVMVILGGMGTLFGPVLGAFILELAHFGLEAVTEHWLLPMGV
HCCCCCHHHHCCCCCCCEEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVITIVLALPKGVAGLLLQWCGKKGEGEP
HHHHHHHHHCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]