BacMap

Definition	Magnetospirillum magneticum AMB-1 chromosome, complete genome.
Accession	NC_007626
Length	4,967,148

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The map label for this gene is braF [H]

Identifier: 83311970

GI number: 83311970

Start: 3071009

End: 3071773

Strand: Reverse

Name: braF [H]

Synonym: amb2871

Alternate gene names: 83311970

Gene position: 3071773-3071009 (Counterclockwise)

Preceding gene: 83311971

Following gene: 83311969

Centisome position: 61.84

GC content: 67.32

Gene sequence:

>765_bases
ATGAGCGTCTCTCCCTTTCTGTTCGAGACCAAGGGCCTGACCAAGGCCTTCGGCGGCCTGATCGCGGTGAATTCGGTGTC
CATCGGTTTTCGGGAGCGCGAGGCCCACGCCATCATCGGCCCCAACGGCGCCGGCAAGAGCACCTTCGTCAACCTGCTGA
CCGGGCACCTGCCGGCCTCGGGCGGGCACGTGATGTTCCGGGGCGCCGACATCACCCGGCTACCGGCCCACCGGGTGGCG
CGCCTCGGCATCGGGCGCTCGTACCAGAAGACCAACATCTTCCCCAGCTTCACCTGCTTCGAGAATTGCCGTCTGGCGGC
CCAGTCGCTGCTGACCACCTCGCTGCGCTTCCTGCGTCCCGCCACCGCCTACCGGGAGGTTTCCGCCAGGGCGTCCGAGG
CGCTGGAGCGGGCCGGGCTGGACTCAAAGGCCGGGCGCATGGCCGGCACCATGTCCTATGGCGAGCAGCGTCAGCTGGAG
ATCGCCATGGTGCTGGCCACCGCGCCCCAGGTCCTGGTGCTCGACGAGCCGCTGGCCGGCATGGGCAAGGAGGAATCCGA
GGGCATGGTCGGCCTGCTGCGGGATCTGGCCGAGACCTACACCCTGATCCTCATCGAGCATGACATGGATGCCGTCTTCG
CCATCGCCAGGGTCCTGTCGGTGATGGTCAACGGCAGCTTGCTGGAAAGCGGGCCCTTAGAGCAGATCCGCGCCAGCAAG
GCGGTTCAGGACGCCTATCTCGGCGACGGGGCGGAGGCATATTGA

Upstream 100 bases:

>100_bases
TGCCCATGGGCGTGCTGGTGATCACCATCGTGCTCGCCCTGCCCAAGGGCGTGGCGGGGTTGTTGCTGCAATGGTGCGGC
AAGAAAGGGGAGGGGGAGCC

Downstream 100 bases:

>100_bases
TGGAAAACGTCCTCGACGCCTCGGGCCTGCACACCTATTACGGCGCCAGCCATATCCTGCACGGCGTGGATTTCCGCGTG
GGGCGGGGTGAGACGGTGGC

Product: ABC-type branched-chain amino acid transport systems, ATPase component

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSVSPFLFETKGLTKAFGGLIAVNSVSIGFREREAHAIIGPNGAGKSTFVNLLTGHLPASGGHVMFRGADITRLPAHRVA
RLGIGRSYQKTNIFPSFTCFENCRLAAQSLLTTSLRFLRPATAYREVSARASEALERAGLDSKAGRMAGTMSYGEQRQLE
IAMVLATAPQVLVLDEPLAGMGKEESEGMVGLLRDLAETYTLILIEHDMDAVFAIARVLSVMVNGSLLESGPLEQIRASK
AVQDAYLGDGAEAY

Sequences:

>Translated_254_residues
MSVSPFLFETKGLTKAFGGLIAVNSVSIGFREREAHAIIGPNGAGKSTFVNLLTGHLPASGGHVMFRGADITRLPAHRVA
RLGIGRSYQKTNIFPSFTCFENCRLAAQSLLTTSLRFLRPATAYREVSARASEALERAGLDSKAGRMAGTMSYGEQRQLE
IAMVLATAPQVLVLDEPLAGMGKEESEGMVGLLRDLAETYTLILIEHDMDAVFAIARVLSVMVNGSLLESGPLEQIRASK
AVQDAYLGDGAEAY
>Mature_253_residues
SVSPFLFETKGLTKAFGGLIAVNSVSIGFREREAHAIIGPNGAGKSTFVNLLTGHLPASGGHVMFRGADITRLPAHRVAR
LGIGRSYQKTNIFPSFTCFENCRLAAQSLLTTSLRFLRPATAYREVSARASEALERAGLDSKAGRMAGTMSYGEQRQLEI
AMVLATAPQVLVLDEPLAGMGKEESEGMVGLLRDLAETYTLILIEHDMDAVFAIARVLSVMVNGSLLESGPLEQIRASKA
VQDAYLGDGAEAY

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI27881501, Length=239, Percent_Identity=30.1255230125523, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI30795238, Length=239, Percent_Identity=30.1255230125523, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI45446740, Length=237, Percent_Identity=28.2700421940928, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI47078218, Length=237, Percent_Identity=28.2700421940928, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI150417984, Length=218, Percent_Identity=33.0275229357798, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI31657092, Length=231, Percent_Identity=29.004329004329, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI105990541, Length=224, Percent_Identity=30.8035714285714, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI21536376, Length=211, Percent_Identity=27.9620853080569, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI153792144, Length=219, Percent_Identity=26.9406392694064, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI116734710, Length=210, Percent_Identity=27.6190476190476, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI62526033, Length=209, Percent_Identity=26.7942583732057, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI27262624, Length=222, Percent_Identity=25.2252252252252, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI27262626, Length=222, Percent_Identity=25.2252252252252, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1789864, Length=250, Percent_Identity=39.2, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI48995001, Length=238, Percent_Identity=29.8319327731092, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790190, Length=223, Percent_Identity=28.6995515695067, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788761, Length=238, Percent_Identity=27.3109243697479, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1788472, Length=238, Percent_Identity=25.6302521008403, Blast_Score=100, Evalue=6e-23,
Organism=Escherichia coli, GI1789593, Length=240, Percent_Identity=29.1666666666667, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1790525, Length=241, Percent_Identity=27.3858921161826, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87081791, Length=233, Percent_Identity=27.4678111587983, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1789991, Length=225, Percent_Identity=28, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1787089, Length=235, Percent_Identity=28.936170212766, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1787370, Length=237, Percent_Identity=27.0042194092827, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786398, Length=263, Percent_Identity=27.7566539923954, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87081709, Length=226, Percent_Identity=26.1061946902655, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1788897, Length=220, Percent_Identity=27.7272727272727, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI87082268, Length=233, Percent_Identity=28.3261802575107, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1788210, Length=246, Percent_Identity=24.390243902439, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787712, Length=224, Percent_Identity=26.7857142857143, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788450, Length=234, Percent_Identity=28.6324786324786, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787029, Length=228, Percent_Identity=25.8771929824561, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787792, Length=240, Percent_Identity=26.25, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI48994943, Length=251, Percent_Identity=27.4900398406374, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI87081834, Length=204, Percent_Identity=29.9019607843137, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788225, Length=237, Percent_Identity=29.1139240506329, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1786872, Length=247, Percent_Identity=25.5060728744939, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1786253, Length=225, Percent_Identity=27.1111111111111, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1786319, Length=234, Percent_Identity=27.3504273504274, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789873, Length=204, Percent_Identity=28.921568627451, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87081782, Length=236, Percent_Identity=28.3898305084746, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789891, Length=211, Percent_Identity=28.436018957346, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1790544, Length=240, Percent_Identity=25.4166666666667, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789672, Length=223, Percent_Identity=22.8699551569507, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1789032, Length=241, Percent_Identity=25.7261410788382, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1786803, Length=239, Percent_Identity=26.7782426778243, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1786563, Length=231, Percent_Identity=25.974025974026, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787500, Length=238, Percent_Identity=26.890756302521, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788506, Length=222, Percent_Identity=25.6756756756757, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787105, Length=220, Percent_Identity=26.3636363636364, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790162, Length=245, Percent_Identity=22.8571428571429, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI48994883, Length=237, Percent_Identity=28.6919831223629, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787758, Length=239, Percent_Identity=25.1046025104602, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1786703, Length=232, Percent_Identity=27.1551724137931, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786654, Length=248, Percent_Identity=25, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1790467, Length=230, Percent_Identity=24.3478260869565, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI145693107, Length=235, Percent_Identity=27.6595744680851, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1789962, Length=235, Percent_Identity=26.3829787234043, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71983523, Length=230, Percent_Identity=29.1304347826087, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71983510, Length=230, Percent_Identity=29.1304347826087, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71983516, Length=230, Percent_Identity=29.1304347826087, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17510237, Length=230, Percent_Identity=23.9130434782609, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17565586, Length=207, Percent_Identity=29.951690821256, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI193211017, Length=220, Percent_Identity=25.4545454545455, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193211015, Length=220, Percent_Identity=25.4545454545455, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71996809, Length=220, Percent_Identity=25.4545454545455, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193202349, Length=219, Percent_Identity=24.6575342465753, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71992285, Length=239, Percent_Identity=24.2677824267782, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17543740, Length=222, Percent_Identity=28.3783783783784, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17541710, Length=208, Percent_Identity=26.9230769230769, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI115533608, Length=201, Percent_Identity=28.3582089552239, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25146777, Length=243, Percent_Identity=25.9259259259259, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319856, Length=227, Percent_Identity=27.3127753303965, Blast_Score=69, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24666092, Length=235, Percent_Identity=28.0851063829787, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI221500365, Length=217, Percent_Identity=25.8064516129032, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI116007184, Length=217, Percent_Identity=25.8064516129032, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24643674, Length=220, Percent_Identity=27.2727272727273, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI116007328, Length=219, Percent_Identity=27.3972602739726, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28574150, Length=219, Percent_Identity=27.3972602739726, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI221512771, Length=220, Percent_Identity=25.4545454545455, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24581191, Length=198, Percent_Identity=31.3131313131313, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24581189, Length=198, Percent_Identity=31.3131313131313, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24581193, Length=198, Percent_Identity=31.3131313131313, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24581195, Length=198, Percent_Identity=31.3131313131313, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24648314, Length=227, Percent_Identity=23.7885462555066, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28574744, Length=204, Percent_Identity=29.4117647058824, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 27208; Mature: 27076

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSPFLFETKGLTKAFGGLIAVNSVSIGFREREAHAIIGPNGAGKSTFVNLLTGHLPAS
CCCCCCEEECCCHHHHHCCEEEEEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHCCCCC
GGHVMFRGADITRLPAHRVARLGIGRSYQKTNIFPSFTCFENCRLAAQSLLTTSLRFLRP
CCEEEEECCCCCCCCHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ATAYREVSARASEALERAGLDSKAGRMAGTMSYGEQRQLEIAMVLATAPQVLVLDEPLAG
HHHHHHHHHHHHHHHHHCCCCCHHCHHHHHCCCCCCHHEEEEEEEECCCEEEEECCCCCC
MGKEESEGMVGLLRDLAETYTLILIEHDMDAVFAIARVLSVMVNGSLLESGPLEQIRASK
CCCCCCCCHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
AVQDAYLGDGAEAY
HHHHHHCCCCCCCC
>Mature Secondary Structure 
SVSPFLFETKGLTKAFGGLIAVNSVSIGFREREAHAIIGPNGAGKSTFVNLLTGHLPAS
CCCCCEEECCCHHHHHCCEEEEEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHCCCCC
GGHVMFRGADITRLPAHRVARLGIGRSYQKTNIFPSFTCFENCRLAAQSLLTTSLRFLRP
CCEEEEECCCCCCCCHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ATAYREVSARASEALERAGLDSKAGRMAGTMSYGEQRQLEIAMVLATAPQVLVLDEPLAG
HHHHHHHHHHHHHHHHHCCCCCHHCHHHHHCCCCCCHHEEEEEEEECCCEEEEECCCCCC
MGKEESEGMVGLLRDLAETYTLILIEHDMDAVFAIARVLSVMVNGSLLESGPLEQIRASK
CCCCCCCCHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
AVQDAYLGDGAEAY
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]