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The map label for this gene is yisK [H]

Identifier: 83311926

GI number: 83311926

Start: 3020614

End: 3021636

Strand: Reverse

Name: yisK [H]

Synonym: amb2827

Alternate gene names: 83311926

Gene position: 3021636-3020614 (Counterclockwise)

Preceding gene: 83311927

Following gene: 83311923

Centisome position: 60.83

GC content: 67.64

Gene sequence:

>1023_bases
ATGGATGAAAAGCGTCGCCAGTTCCTGACCCTGGGGGCCGGAGTGGCCGCCGGTGCCTTGTTGACACGGGCGGCCGGCGC
CGCCGGTCCGATCCCCAATTCCGCCGCCGGTGCGCTGCGGCTGGTGACTTATTCGGCCGGGGGGGTGGCACCGCGCGTCG
GCGTGGTCAAGGCTTCGGGCCGGGTGGTTGACCTGGGGGCGGCGGCCAAGGCCCGGGGCATGGCCTTGGCCTTCGATCCG
GCCAGCATGGTCTCGCTGATCCGGGCCGGCGACTTCGCCCTGGCCCAGGCCCGGCAATTGATGCAGGAGGGGCCGGAGAC
CGGCCCGCTGGTCAGCGAGGTGCGGCTGCTGGCCCCCATCCCCGAGCCCAGCCGCAACGTCTATGCCGTGGGCTGGAACT
ATCTCGAGCACTTCAAGGAAGGCGAGGCCATGCGCCTCAAATCCGCCGACCTGCCCGAGCATCCGGTGTTCTTCACCAAG
GCGGTGGGCACCGTCAACGGTCCCTATGATCCGATTCCCTATGACGCCGCCGTTTCCACCTCCATCGACTGGGAGTGCGA
ACTGGCGGTGATCATCGGCAAGGGGGGCAAAAACATCGCCGAGGCCGACGCCATGAAGCACGTCTTCGGCTTTTGCGTCA
TCAACGACACCACGGCCCGCGATGTGCAGCAGAAGAAGCACGGCGGCCAGTGGTTCAAGGGCAAGAGCCTGGACGGCCAC
GGGCCGCTGGGGCCGTGGATCGTGCCGGCCTCCGACATCGACCACACCCGGGTCCATCTGATCACCCGGGTCAACGGGGT
GGTCAAGCAGGACGCCAGCACCGAGCAAATGTACTTCAAGGTACCGCGCATCATCGCCGAGCTGTCGGCGGGCCTAACCC
TGGAGCCGGGCGACATCATCGCCACCGGTACGCCCCCCGGCGTCGGCGGAGCGCGCAAGCCGCCGGAATTCCTCAAACCC
GGCGATGTGATGGAAACCGAGATCGTCGAGATCGGCCTGCTGCGGAACGTGATCGGCGGGTAG

Upstream 100 bases:

>100_bases
CGTGGTCATCGGCTTCATCGACGAACTGGAGGCCATGTAGGCAAGGCGCGGGCGGCTTCCCATATGGCAGGGAGCAAAAG
CCTGCCATAGGGAGACCGTC

Downstream 100 bases:

>100_bases
GGCGGGGGATCACCCCGCCATGCGGGATTCCTCCAGGGTGGCAAGCTCGGTGGCCAGGGTCTCCAGCATGGTGACCACGG
CCACCGAGGCCTTGTTCATG

Product: 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MDEKRRQFLTLGAGVAAGALLTRAAGAAGPIPNSAAGALRLVTYSAGGVAPRVGVVKASGRVVDLGAAAKARGMALAFDP
ASMVSLIRAGDFALAQARQLMQEGPETGPLVSEVRLLAPIPEPSRNVYAVGWNYLEHFKEGEAMRLKSADLPEHPVFFTK
AVGTVNGPYDPIPYDAAVSTSIDWECELAVIIGKGGKNIAEADAMKHVFGFCVINDTTARDVQQKKHGGQWFKGKSLDGH
GPLGPWIVPASDIDHTRVHLITRVNGVVKQDASTEQMYFKVPRIIAELSAGLTLEPGDIIATGTPPGVGGARKPPEFLKP
GDVMETEIVEIGLLRNVIGG

Sequences:

>Translated_340_residues
MDEKRRQFLTLGAGVAAGALLTRAAGAAGPIPNSAAGALRLVTYSAGGVAPRVGVVKASGRVVDLGAAAKARGMALAFDP
ASMVSLIRAGDFALAQARQLMQEGPETGPLVSEVRLLAPIPEPSRNVYAVGWNYLEHFKEGEAMRLKSADLPEHPVFFTK
AVGTVNGPYDPIPYDAAVSTSIDWECELAVIIGKGGKNIAEADAMKHVFGFCVINDTTARDVQQKKHGGQWFKGKSLDGH
GPLGPWIVPASDIDHTRVHLITRVNGVVKQDASTEQMYFKVPRIIAELSAGLTLEPGDIIATGTPPGVGGARKPPEFLKP
GDVMETEIVEIGLLRNVIGG
>Mature_340_residues
MDEKRRQFLTLGAGVAAGALLTRAAGAAGPIPNSAAGALRLVTYSAGGVAPRVGVVKASGRVVDLGAAAKARGMALAFDP
ASMVSLIRAGDFALAQARQLMQEGPETGPLVSEVRLLAPIPEPSRNVYAVGWNYLEHFKEGEAMRLKSADLPEHPVFFTK
AVGTVNGPYDPIPYDAAVSTSIDWECELAVIIGKGGKNIAEADAMKHVFGFCVINDTTARDVQQKKHGGQWFKGKSLDGH
GPLGPWIVPASDIDHTRVHLITRVNGVVKQDASTEQMYFKVPRIIAELSAGLTLEPGDIIATGTPPGVGGARKPPEFLKP
GDVMETEIVEIGLLRNVIGG

Specific function: Unknown

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=313, Percent_Identity=39.6166134185303, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI40786394, Length=324, Percent_Identity=38.8888888888889, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI66348062, Length=205, Percent_Identity=36.5853658536585, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI215422413, Length=205, Percent_Identity=36.5853658536585, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI13654274, Length=205, Percent_Identity=36.5853658536585, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1787428, Length=193, Percent_Identity=38.3419689119171, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17557057, Length=202, Percent_Identity=35.6435643564356, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324161, Length=241, Percent_Identity=29.045643153527, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28572127, Length=305, Percent_Identity=38.6885245901639, Blast_Score=185, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24663695, Length=307, Percent_Identity=32.8990228013029, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI28571789, Length=212, Percent_Identity=31.6037735849057, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: NA

Molecular weight: Translated: 35828; Mature: 35828

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEKRRQFLTLGAGVAAGALLTRAAGAAGPIPNSAAGALRLVTYSAGGVAPRVGVVKASG
CCHHHHHHEECCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEECCC
RVVDLGAAAKARGMALAFDPASMVSLIRAGDFALAQARQLMQEGPETGPLVSEVRLLAPI
EEEEECCCHHHCCEEEEECHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHEEECC
PEPSRNVYAVGWNYLEHFKEGEAMRLKSADLPEHPVFFTKAVGTVNGPYDPIPYDAAVST
CCCCCCEEEECHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCEECC
SIDWECELAVIIGKGGKNIAEADAMKHVFGFCVINDTTARDVQQKKHGGQWFKGKSLDGH
CCCCEEEEEEEEECCCCCHHHHHHHHHHHEEEEECCCHHHHHHHHHCCCCEECCCCCCCC
GPLGPWIVPASDIDHTRVHLITRVNGVVKQDASTEQMYFKVPRIIAELSAGLTLEPGDII
CCCCCEEEECCCCCCHHEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEECCCCEE
ATGTPPGVGGARKPPEFLKPGDVMETEIVEIGLLRNVIGG
ECCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDEKRRQFLTLGAGVAAGALLTRAAGAAGPIPNSAAGALRLVTYSAGGVAPRVGVVKASG
CCHHHHHHEECCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEECCC
RVVDLGAAAKARGMALAFDPASMVSLIRAGDFALAQARQLMQEGPETGPLVSEVRLLAPI
EEEEECCCHHHCCEEEEECHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHEEECC
PEPSRNVYAVGWNYLEHFKEGEAMRLKSADLPEHPVFFTKAVGTVNGPYDPIPYDAAVST
CCCCCCEEEECHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCEECC
SIDWECELAVIIGKGGKNIAEADAMKHVFGFCVINDTTARDVQQKKHGGQWFKGKSLDGH
CCCCEEEEEEEEECCCCCHHHHHHHHHHHEEEEECCCHHHHHHHHHCCCCEECCCCCCCC
GPLGPWIVPASDIDHTRVHLITRVNGVVKQDASTEQMYFKVPRIIAELSAGLTLEPGDII
CCCCCEEEECCCCCCHHEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEECCCCEE
ATGTPPGVGGARKPPEFLKPGDVMETEIVEIGLLRNVIGG
ECCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377 [H]