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The map label for this gene is pntA

Identifier: 83311923

GI number: 83311923

Start: 3015573

End: 3017159

Strand: Reverse

Name: pntA

Synonym: amb2824

Alternate gene names: 83311923

Gene position: 3017159-3015573 (Counterclockwise)

Preceding gene: 83311926

Following gene: 83311922

Centisome position: 60.74

GC content: 67.04

Gene sequence:

>1587_bases
ATGTCTCTGACCATTGGTGTTCCAAGGGAAACGTTCCCCAACGAGAAGCGGGTGGCGAGCGTTCCCGATGTCGTCGAGAA
GCTGATCAAGCTCGGCTTCTCCGTGGTGGTCGAGACCGGTGCCGGCGCACTCGCCAATTTCGATGACGAGTCTTATGTGG
CCGCTGGTGCCACCATCGCCAAGAGTGCCGCCGAGCTTTGGGCCGCCTCCGGCATGGTCTTCAAGGTCCGCGCCCCGTCC
ATGGACGAAGTGGCGCTGATCAAGGAGGGCACCACCCTGATCGGCTTCGTCTGGCCGGCCCAGAATCCCGAGCTGATGCA
GGCCCTGGCGGCGCGCAAGATCACCGTGCTGGCCATGGATTGCGTGCCGCGCCAGCTCTCGCGCGCCCAGAAGATGGACG
CCCTGTCCTCCATGGCCAATATCGCCGGCTATCGCGCCGTGGTCGAGGCGGCGCACCAGTTCGGCCGCTTCTTCACCGGC
CAGATCACCGCCGCGGGCAAGGTTCCCCCGGCCAAGGTCTTCGTCGCCGGCGCCGGCGTGGCCGGCTTGGCCGCCATCGG
CACCGCCGTGGGACTGGGCGCCGTGGTGCGCGCCAACGACACCCGCGCCGAGGTGGCCGATCAGGTCAAGTCCATGGGCG
CCGAATTCGTCAAGGTGGAGTACGAGGAAGAGGGCTCGGGCGGCGGCGGCTACGCCAAGGTCATGAGCGAGGGCTTCCAG
GCCGCTCAGCGCGAGATGTACGCCCAGCAGGCGCGCGAGGTGGACATCGTCATCACCACCGCCCTGATCCCCGGCAAGCC
GGCGCCCCGGCTGATCACCGCTGAGATGGTCCAGAGCATGCGGGCCGGCAGCGTCATCGTCGACATGGCCGCCGAGCAGG
GCGGCAATTGCGAGCTGACCGTGCCGGGCGAGGTGGTGGTGCGCCATGGCGTGACCATCGTCGGCTATGCCGACCTGCCC
AGCCGCCTGTCCAAGCAGGCCTCGACGCTGTACGCCACCAATCTGCTGCGCCTCACCGAGGAGCTGTGCAAGACCAAGGA
CGGCATCATCGACGTCAATTTCGGCGACGATGCCATCCGCGGCCTGACGGTCATCAAGGACGGCGAGATCACCTGGCCGG
CGCCGCCCCTGGTTCTGCCGGCGCCGCCCGCCCCCAAGCCGGCCGCCGCCATGCCCGAGAAGAAGGCGTCGGGCCACGGC
CACGGCGCGGGCGAGCCCATGTCGGCCAAATCCCTGGCCATCACCTTCGGCATCGGTGCCCTGGCCTTCTGGTTCGTGGG
CGCCAATGCCCCCGAGGCCTTCCTGGGCCACTTCACCGTGTTCGTGCTGGCCTGCTTCGTGGGCTACATGGTGGTGTGGA
ACGTTACCCCCGCGCTGCATACCCCGCTGATGAGCGTCACCAACGCCATCTCGTCGATCATCGTCATCGGTGCCCTGGTT
CAGATTTCTCCGCCGCTGGGCGAGGGGATCTCCAGGCCCGGCTTCTGGATCGGCCTGCTGGCGGTGATCAGCATCATTCT
CACCTCGATCAACATGTTCGGTGGCTTCGCGGTCACCAAGCGCATGTTGGCGATGTTCCGCAAGTAA

Upstream 100 bases:

>100_bases
ATGATCAGGCTTGGCAAACCGCTGCCCAGAGGGCATTGTTTTCCGCAAAATAGTGCGTGCGCGCAATGACAAGCAGTGAC
AACGAACAACAGGAGAAGCC

Downstream 100 bases:

>100_bases
GGGGGGCACCATGTCCGCAAGTCTCGCCACCGTTTCCTATATCGGTGCCACCATCCTGTTCATCCTCAGCCTCGGCGGCC
TGTCCAATCCCGAGACCTCG

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MSLTIGVPRETFPNEKRVASVPDVVEKLIKLGFSVVVETGAGALANFDDESYVAAGATIAKSAAELWAASGMVFKVRAPS
MDEVALIKEGTTLIGFVWPAQNPELMQALAARKITVLAMDCVPRQLSRAQKMDALSSMANIAGYRAVVEAAHQFGRFFTG
QITAAGKVPPAKVFVAGAGVAGLAAIGTAVGLGAVVRANDTRAEVADQVKSMGAEFVKVEYEEEGSGGGGYAKVMSEGFQ
AAQREMYAQQAREVDIVITTALIPGKPAPRLITAEMVQSMRAGSVIVDMAAEQGGNCELTVPGEVVVRHGVTIVGYADLP
SRLSKQASTLYATNLLRLTEELCKTKDGIIDVNFGDDAIRGLTVIKDGEITWPAPPLVLPAPPAPKPAAAMPEKKASGHG
HGAGEPMSAKSLAITFGIGALAFWFVGANAPEAFLGHFTVFVLACFVGYMVVWNVTPALHTPLMSVTNAISSIIVIGALV
QISPPLGEGISRPGFWIGLLAVISIILTSINMFGGFAVTKRMLAMFRK

Sequences:

>Translated_528_residues
MSLTIGVPRETFPNEKRVASVPDVVEKLIKLGFSVVVETGAGALANFDDESYVAAGATIAKSAAELWAASGMVFKVRAPS
MDEVALIKEGTTLIGFVWPAQNPELMQALAARKITVLAMDCVPRQLSRAQKMDALSSMANIAGYRAVVEAAHQFGRFFTG
QITAAGKVPPAKVFVAGAGVAGLAAIGTAVGLGAVVRANDTRAEVADQVKSMGAEFVKVEYEEEGSGGGGYAKVMSEGFQ
AAQREMYAQQAREVDIVITTALIPGKPAPRLITAEMVQSMRAGSVIVDMAAEQGGNCELTVPGEVVVRHGVTIVGYADLP
SRLSKQASTLYATNLLRLTEELCKTKDGIIDVNFGDDAIRGLTVIKDGEITWPAPPLVLPAPPAPKPAAAMPEKKASGHG
HGAGEPMSAKSLAITFGIGALAFWFVGANAPEAFLGHFTVFVLACFVGYMVVWNVTPALHTPLMSVTNAISSIIVIGALV
QISPPLGEGISRPGFWIGLLAVISIILTSINMFGGFAVTKRMLAMFRK
>Mature_527_residues
SLTIGVPRETFPNEKRVASVPDVVEKLIKLGFSVVVETGAGALANFDDESYVAAGATIAKSAAELWAASGMVFKVRAPSM
DEVALIKEGTTLIGFVWPAQNPELMQALAARKITVLAMDCVPRQLSRAQKMDALSSMANIAGYRAVVEAAHQFGRFFTGQ
ITAAGKVPPAKVFVAGAGVAGLAAIGTAVGLGAVVRANDTRAEVADQVKSMGAEFVKVEYEEEGSGGGGYAKVMSEGFQA
AQREMYAQQAREVDIVITTALIPGKPAPRLITAEMVQSMRAGSVIVDMAAEQGGNCELTVPGEVVVRHGVTIVGYADLPS
RLSKQASTLYATNLLRLTEELCKTKDGIIDVNFGDDAIRGLTVIKDGEITWPAPPLVLPAPPAPKPAAAMPEKKASGHGH
GAGEPMSAKSLAITFGIGALAFWFVGANAPEAFLGHFTVFVLACFVGYMVVWNVTPALHTPLMSVTNAISSIIVIGALVQ
ISPPLGEGISRPGFWIGLLAVISIILTSINMFGGFAVTKRMLAMFRK

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=544, Percent_Identity=50.1838235294118, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI122939153, Length=544, Percent_Identity=50.1838235294118, Blast_Score=473, Evalue=1e-133,
Organism=Escherichia coli, GI1787887, Length=527, Percent_Identity=60.1518026565465, Blast_Score=614, Evalue=1e-177,
Organism=Caenorhabditis elegans, GI17550456, Length=526, Percent_Identity=50.3802281368821, Blast_Score=468, Evalue=1e-132,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 55371; Mature: 55240

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTIGVPRETFPNEKRVASVPDVVEKLIKLGFSVVVETGAGALANFDDESYVAAGATIA
CEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCEECCCCCCCEECCHHHH
KSAAELWAASGMVFKVRAPSMDEVALIKEGTTLIGFVWPAQNPELMQALAARKITVLAMD
HHHHHHHHCCCEEEEEECCCCCCEEEEECCCEEEEEEECCCCHHHHHHHHHHHEEEEEEH
CVPRQLSRAQKMDALSSMANIAGYRAVVEAAHQFGRFFTGQITAAGKVPPAKVFVAGAGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEECCCH
AGLAAIGTAVGLGAVVRANDTRAEVADQVKSMGAEFVKVEYEEEGSGGGGYAKVMSEGFQ
HHHHHHHHHHHCCEEEECCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHH
AAQREMYAQQAREVDIVITTALIPGKPAPRLITAEMVQSMRAGSVIVDMAAEQGGNCELT
HHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEHHCCCCCEEEE
VPGEVVVRHGVTIVGYADLPSRLSKQASTLYATNLLRLTEELCKTKDGIIDVNFGDDAIR
CCHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHC
GLTVIKDGEITWPAPPLVLPAPPAPKPAAAMPEKKASGHGHGAGEPMSAKSLAITFGIGA
CEEEEECCCEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHH
LAFWFVGANAPEAFLGHFTVFVLACFVGYMVVWNVTPALHTPLMSVTNAISSIIVIGALV
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
QISPPLGEGISRPGFWIGLLAVISIILTSINMFGGFAVTKRMLAMFRK
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLTIGVPRETFPNEKRVASVPDVVEKLIKLGFSVVVETGAGALANFDDESYVAAGATIA
EEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCEECCCCCCCEECCHHHH
KSAAELWAASGMVFKVRAPSMDEVALIKEGTTLIGFVWPAQNPELMQALAARKITVLAMD
HHHHHHHHCCCEEEEEECCCCCCEEEEECCCEEEEEEECCCCHHHHHHHHHHHEEEEEEH
CVPRQLSRAQKMDALSSMANIAGYRAVVEAAHQFGRFFTGQITAAGKVPPAKVFVAGAGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEECCCH
AGLAAIGTAVGLGAVVRANDTRAEVADQVKSMGAEFVKVEYEEEGSGGGGYAKVMSEGFQ
HHHHHHHHHHHCCEEEECCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHH
AAQREMYAQQAREVDIVITTALIPGKPAPRLITAEMVQSMRAGSVIVDMAAEQGGNCELT
HHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEHHCCCCCEEEE
VPGEVVVRHGVTIVGYADLPSRLSKQASTLYATNLLRLTEELCKTKDGIIDVNFGDDAIR
CCHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHC
GLTVIKDGEITWPAPPLVLPAPPAPKPAAAMPEKKASGHGHGAGEPMSAKSLAITFGIGA
CEEEEECCCEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHH
LAFWFVGANAPEAFLGHFTVFVLACFVGYMVVWNVTPALHTPLMSVTNAISSIIVIGALV
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
QISPPLGEGISRPGFWIGLLAVISIILTSINMFGGFAVTKRMLAMFRK
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]