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The map label for this gene is pntB [H]

Identifier: 83311922

GI number: 83311922

Start: 3014153

End: 3015562

Strand: Reverse

Name: pntB [H]

Synonym: amb2823

Alternate gene names: 83311922

Gene position: 3015562-3014153 (Counterclockwise)

Preceding gene: 83311923

Following gene: 83311921

Centisome position: 60.71

GC content: 64.68

Gene sequence:

>1410_bases
ATGTCCGCAAGTCTCGCCACCGTTTCCTATATCGGTGCCACCATCCTGTTCATCCTCAGCCTCGGCGGCCTGTCCAATCC
CGAGACCTCGCGCCGCGGCAACGTGCTCGGCATGATCGGCATGGCCCTGGCGGTGATCGCCACCGTGTTCGGTCCCCAGG
TCACCTGGGGCGGGGTGCCGTGGATCATCATCGCCATGGCCATCGGCGGCTCGGTCGGTCTTTACGCCGCCCGCACCGTG
CAGATGACCCAGATGCCCGAACTGGTCGCCCTGATGCACTCCCTGGTCGGCCTCGCCGCCTGCCTGGTGGGCTTCGCCAG
CTACATCGACACCGCCCACACCTTCGTCGGCGCCGAGAAGACCATCCATGACGTGGAGATCTATATCGGCATTCTGATCG
GTGCCGTGACCTTCTCGGGCTCGCTGATCGCCTTCGGCAAGCTGTCGGCCAAGATCGGCGGCAAGCCGCTGCTGCTGCCC
GCCCGCCATCTGCTCAATCTGGCCGGCCTGCTGGTGGTGATCTGGGTCGGCAAGGGCTTCCTCAACGCCGAAAGCACCGG
CGGCGGCTTCTTCGCCCTGATCATCATGACCCTGATCGCCCTGGCCTTCGGCGTGCACATGGTGATGGCCATCGGCGGCG
CCGACATGCCCGTGGTGGTTTCCATGCTGAACAGCTACTCGGGCTGGGCGGCGGCGGCCACCGGCTTCATGCTGAACAAC
GACCTGCTGATCGTCACCGGCGCCCTGGTGGGCTCCTCGGGCGCCATCCTGTCGTACATCATGTGCCGGGCCATGAACCG
CAACTTCATCAGCGTCATCGCCGGCGGCTTCGGCACCGGCGGCGGCACGGTGGCGGCTCCGGCCGATGGCCAGCCGGCCG
GCGAGGTCATCCCGGTCAGCGCCACCGAGACAGCCGAGCTGCTGAAGGACGCCAAGAGCGTGATCATCGTTCCCGGCTAC
GGCATGGCGGTGGCCCAGGCCCAGCACACGGTCTACGAGATCACCAAGCATCTGCGTGAGAAGGGCGTCAATATCCGCTT
CGGCATTCATCCGGTGGCGGGCCGCATGCCCGGTCACATGAACGTGCTGCTGGCCGAAGCCAAGGTGCCCTACGACATCG
TCATGGAGATGGACGAGATCAATGACGACTTCCCCGAGACGGATGTCGCCATGGTCATCGGCGCCAACGACATCGTCAAC
CCGGCAGCGCAGGAGGATCCCAATTCTCCCATCGCTGGCATGCCGGTGCTGGAGGTGTGGAAGGCCAAGACCTCCATCGT
CATGAAGCGTTCCATGGCGTCCGGCTATGCCGGCGTCGACAATCCGTTGTTCTACAAGGAGAACAACCGGATGCTGTTCG
GCGATGCCAAGAAGATGCTGGACGAGGTGCTGACTGCCTTGAAGGCCTGA

Upstream 100 bases:

>100_bases
CTGGCGGTGATCAGCATCATTCTCACCTCGATCAACATGTTCGGTGGCTTCGCGGTCACCAAGCGCATGTTGGCGATGTT
CCGCAAGTAAGGGGGGCACC

Downstream 100 bases:

>100_bases
AGCGCCGAGGGGCAGCGGGAAAACTCCACTTCGGGGGCTTTCGCCCCCGATCCCCCAACCGGGAAGCACAGCTTCCCGAA
CCCTTCAGCTCTTTAATAGA

Product: NAD/NADP transhydrogenase beta subunit

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Pyridine nucleotide transhydrogenase subunit beta [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSASLATVSYIGATILFILSLGGLSNPETSRRGNVLGMIGMALAVIATVFGPQVTWGGVPWIIIAMAIGGSVGLYAARTV
QMTQMPELVALMHSLVGLAACLVGFASYIDTAHTFVGAEKTIHDVEIYIGILIGAVTFSGSLIAFGKLSAKIGGKPLLLP
ARHLLNLAGLLVVIWVGKGFLNAESTGGGFFALIIMTLIALAFGVHMVMAIGGADMPVVVSMLNSYSGWAAAATGFMLNN
DLLIVTGALVGSSGAILSYIMCRAMNRNFISVIAGGFGTGGGTVAAPADGQPAGEVIPVSATETAELLKDAKSVIIVPGY
GMAVAQAQHTVYEITKHLREKGVNIRFGIHPVAGRMPGHMNVLLAEAKVPYDIVMEMDEINDDFPETDVAMVIGANDIVN
PAAQEDPNSPIAGMPVLEVWKAKTSIVMKRSMASGYAGVDNPLFYKENNRMLFGDAKKMLDEVLTALKA

Sequences:

>Translated_469_residues
MSASLATVSYIGATILFILSLGGLSNPETSRRGNVLGMIGMALAVIATVFGPQVTWGGVPWIIIAMAIGGSVGLYAARTV
QMTQMPELVALMHSLVGLAACLVGFASYIDTAHTFVGAEKTIHDVEIYIGILIGAVTFSGSLIAFGKLSAKIGGKPLLLP
ARHLLNLAGLLVVIWVGKGFLNAESTGGGFFALIIMTLIALAFGVHMVMAIGGADMPVVVSMLNSYSGWAAAATGFMLNN
DLLIVTGALVGSSGAILSYIMCRAMNRNFISVIAGGFGTGGGTVAAPADGQPAGEVIPVSATETAELLKDAKSVIIVPGY
GMAVAQAQHTVYEITKHLREKGVNIRFGIHPVAGRMPGHMNVLLAEAKVPYDIVMEMDEINDDFPETDVAMVIGANDIVN
PAAQEDPNSPIAGMPVLEVWKAKTSIVMKRSMASGYAGVDNPLFYKENNRMLFGDAKKMLDEVLTALKA
>Mature_468_residues
SASLATVSYIGATILFILSLGGLSNPETSRRGNVLGMIGMALAVIATVFGPQVTWGGVPWIIIAMAIGGSVGLYAARTVQ
MTQMPELVALMHSLVGLAACLVGFASYIDTAHTFVGAEKTIHDVEIYIGILIGAVTFSGSLIAFGKLSAKIGGKPLLLPA
RHLLNLAGLLVVIWVGKGFLNAESTGGGFFALIIMTLIALAFGVHMVMAIGGADMPVVVSMLNSYSGWAAAATGFMLNND
LLIVTGALVGSSGAILSYIMCRAMNRNFISVIAGGFGTGGGTVAAPADGQPAGEVIPVSATETAELLKDAKSVIIVPGYG
MAVAQAQHTVYEITKHLREKGVNIRFGIHPVAGRMPGHMNVLLAEAKVPYDIVMEMDEINDDFPETDVAMVIGANDIVNP
AAQEDPNSPIAGMPVLEVWKAKTSIVMKRSMASGYAGVDNPLFYKENNRMLFGDAKKMLDEVLTALKA

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=474, Percent_Identity=51.4767932489451, Blast_Score=448, Evalue=1e-126,
Organism=Homo sapiens, GI122939153, Length=474, Percent_Identity=51.4767932489451, Blast_Score=448, Evalue=1e-126,
Organism=Escherichia coli, GI1787886, Length=467, Percent_Identity=66.3811563169165, Blast_Score=612, Evalue=1e-176,
Organism=Caenorhabditis elegans, GI17550456, Length=466, Percent_Identity=48.9270386266094, Blast_Score=420, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 48985; Mature: 48854

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASLATVSYIGATILFILSLGGLSNPETSRRGNVLGMIGMALAVIATVFGPQVTWGGVP
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCH
WIIIAMAIGGSVGLYAARTVQMTQMPELVALMHSLVGLAACLVGFASYIDTAHTFVGAEK
HHHHHHHHCCCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
TIHDVEIYIGILIGAVTFSGSLIAFGKLSAKIGGKPLLLPARHLLNLAGLLVVIWVGKGF
HHHHHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCC
LNAESTGGGFFALIIMTLIALAFGVHMVMAIGGADMPVVVSMLNSYSGWAAAATGFMLNN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCEEECC
DLLIVTGALVGSSGAILSYIMCRAMNRNFISVIAGGFGTGGGTVAAPADGQPAGEVIPVS
CEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCCCCCCEEECC
ATETAELLKDAKSVIIVPGYGMAVAQAQHTVYEITKHLREKGVNIRFGIHPVAGRMPGHM
CHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECEECCCCCCCCC
NVLLAEAKVPYDIVMEMDEINDDFPETDVAMVIGANDIVNPAAQEDPNSPIAGMPVLEVW
EEEEECCCCCHHHEEEHHHCCCCCCCCCEEEEECCCHHCCCCCCCCCCCCCCCCCHHHHH
KAKTSIVMKRSMASGYAGVDNPLFYKENNRMLFGDAKKMLDEVLTALKA
HHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SASLATVSYIGATILFILSLGGLSNPETSRRGNVLGMIGMALAVIATVFGPQVTWGGVP
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCH
WIIIAMAIGGSVGLYAARTVQMTQMPELVALMHSLVGLAACLVGFASYIDTAHTFVGAEK
HHHHHHHHCCCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
TIHDVEIYIGILIGAVTFSGSLIAFGKLSAKIGGKPLLLPARHLLNLAGLLVVIWVGKGF
HHHHHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCC
LNAESTGGGFFALIIMTLIALAFGVHMVMAIGGADMPVVVSMLNSYSGWAAAATGFMLNN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCEEECC
DLLIVTGALVGSSGAILSYIMCRAMNRNFISVIAGGFGTGGGTVAAPADGQPAGEVIPVS
CEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCCCCCCEEECC
ATETAELLKDAKSVIIVPGYGMAVAQAQHTVYEITKHLREKGVNIRFGIHPVAGRMPGHM
CHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECEECCCCCCCCC
NVLLAEAKVPYDIVMEMDEINDDFPETDVAMVIGANDIVNPAAQEDPNSPIAGMPVLEVW
EEEEECCCCCHHHEEEHHHCCCCCCCCCEEEEECCCHHCCCCCCCCCCCCCCCCCHHHHH
KAKTSIVMKRSMASGYAGVDNPLFYKENNRMLFGDAKKMLDEVLTALKA
HHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]