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The map label for this gene is prfB

Identifier: 83311912

GI number: 83311912

Start: 3004357

End: 3005472

Strand: Reverse

Name: prfB

Synonym: amb2813

Alternate gene names: 83311912

Gene position: 3005472-3004357 (Counterclockwise)

Preceding gene: 83311913

Following gene: 83311911

Centisome position: 60.51

GC content: 64.87

Gene sequence:

>1116_bases
ATGCGGGCCGAGATCGAGGCGCTCGCCCAGGATATCCGGCGATCAGCCGCCCTGTTGAAGCGTCATCTCAATTGGGACGA
GGCGTTGATGCGGCTCGACGAGCTGAACGCTTCGGCCGAGAATCCCGATCTGTGGAACGACGCCGGCGCGGCGCAGAAGA
TCATGCGCGAGCGCAACGAGCTGGACAGCGCCATTCAGGGCTGCCGCGCCCTGGAGCGCGAACTGGCCGATCTGGCGGAA
CTGATCGAGCTGGGCGAGATGGAAGGCGACCAGACGGTCATCGACGACGCCGAGGAACAGGTTCGCGCCCTGAAGGAGCG
CGCCGCCAAGATGGAGCTGGAAACCCTGCTCTCGGGCGAGGCCGACCACAACGATTGCTACATGGAAATCAATGCCGGCG
CCGGCGGCACCGAATCCCAGGACTGGGCCGAGATGCTGCTGCGCATGTACACCCGCTGGGCGGAAAAGCACGGCTACAAG
GTGGAATGGCTGGAAGAAAGCGCCGGCGAGCAGGCGGGCATCAAGTCGGCCACCATCCGCATTCTCGGCCATAATGCCTA
TGGCTGGCTGAAGACCGAAAGCGGCGTGCACCGGCTGGTGCGCATCTCGCCCTATGACAGCGCGGCGCGCCGCCACACCA
GCTTCTCCTCCGCCTGGGTCTATCCGGTGATCGACGACACCATCGATATCCAGATCAACGAGAGCGAGTGCCGCATCGAC
ACCTACCGCGCCTCGGGCGCCGGCGGCCAGCACATCAACAAGACCGATTCGGCGGTGCGTATCACCCATATTCCCACCGG
CATCGCCGTGGCCTGCCAGATGGAACGGTCACAGCACCAGAACCGGGCGCGGGCCTGGGACATGCTGCGCGCCCGCCTGT
ACGAGGCCGAACTGCAAAAGCGCGAAGCCGCCGCCCAGGCGCTGGAAGACCAGAAGACTGATATTGGCTGGGGCCACCAG
ATCCGCTCCTACGTGCTGCAGCCCTACCAGATGGTCAAGGACCTGCGCACCAATGTGGAGACCTCGGACACCCAGGGTGT
TCTCGACGGCGACCTGGACATGTTCATGGCCGCCTCGCTGGCCGCGCGGGTCCAGGGACAGGTGGATCAGGGTTAA

Upstream 100 bases:

>100_bases
GCCGCCGCGCTGATCTCAGGGGGGCGGCAATCTCTCGGGTTGCCAGCCAGGGCGGACTTCCTTACAAAGGGCGCCTCCAT
TCAGCCTTAGGGGAACAGCC

Downstream 100 bases:

>100_bases
CGGGCGGCGGAACAACTCCCCTTCGGGGGCTTTTGCCCCCGATCCCCCAACCGGGAAGCACAGCTTCCCGGACCCTTCTG
TTTTGAAATAAAGAGAAACT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MRAEIEALAQDIRRSAALLKRHLNWDEALMRLDELNASAENPDLWNDAGAAQKIMRERNELDSAIQGCRALERELADLAE
LIELGEMEGDQTVIDDAEEQVRALKERAAKMELETLLSGEADHNDCYMEINAGAGGTESQDWAEMLLRMYTRWAEKHGYK
VEWLEESAGEQAGIKSATIRILGHNAYGWLKTESGVHRLVRISPYDSAARRHTSFSSAWVYPVIDDTIDIQINESECRID
TYRASGAGGQHINKTDSAVRITHIPTGIAVACQMERSQHQNRARAWDMLRARLYEAELQKREAAAQALEDQKTDIGWGHQ
IRSYVLQPYQMVKDLRTNVETSDTQGVLDGDLDMFMAASLAARVQGQVDQG

Sequences:

>Translated_371_residues
MRAEIEALAQDIRRSAALLKRHLNWDEALMRLDELNASAENPDLWNDAGAAQKIMRERNELDSAIQGCRALERELADLAE
LIELGEMEGDQTVIDDAEEQVRALKERAAKMELETLLSGEADHNDCYMEINAGAGGTESQDWAEMLLRMYTRWAEKHGYK
VEWLEESAGEQAGIKSATIRILGHNAYGWLKTESGVHRLVRISPYDSAARRHTSFSSAWVYPVIDDTIDIQINESECRID
TYRASGAGGQHINKTDSAVRITHIPTGIAVACQMERSQHQNRARAWDMLRARLYEAELQKREAAAQALEDQKTDIGWGHQ
IRSYVLQPYQMVKDLRTNVETSDTQGVLDGDLDMFMAASLAARVQGQVDQG
>Mature_371_residues
MRAEIEALAQDIRRSAALLKRHLNWDEALMRLDELNASAENPDLWNDAGAAQKIMRERNELDSAIQGCRALERELADLAE
LIELGEMEGDQTVIDDAEEQVRALKERAAKMELETLLSGEADHNDCYMEINAGAGGTESQDWAEMLLRMYTRWAEKHGYK
VEWLEESAGEQAGIKSATIRILGHNAYGWLKTESGVHRLVRISPYDSAARRHTSFSSAWVYPVIDDTIDIQINESECRID
TYRASGAGGQHINKTDSAVRITHIPTGIAVACQMERSQHQNRARAWDMLRARLYEAELQKREAAAQALEDQKTDIGWGHQ
IRSYVLQPYQMVKDLRTNVETSDTQGVLDGDLDMFMAASLAARVQGQVDQG

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=316, Percent_Identity=34.8101265822785, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI34577120, Length=310, Percent_Identity=34.5161290322581, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI166795305, Length=223, Percent_Identity=34.5291479820628, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI2367172, Length=353, Percent_Identity=54.6742209631728, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI1787462, Length=309, Percent_Identity=38.1877022653722, Blast_Score=191, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI17542784, Length=207, Percent_Identity=34.7826086956522, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321295, Length=233, Percent_Identity=38.6266094420601, Blast_Score=141, Evalue=1e-34,
Organism=Drosophila melanogaster, GI19921226, Length=274, Percent_Identity=37.2262773722628, Blast_Score=157, Evalue=9e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF2_MAGSA (Q2W3F8)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_422176.1
- HSSP:   P0A7I0
- ProteinModelPortal:   Q2W3F8
- SMR:   Q2W3F8
- STRING:   Q2W3F8
- GeneID:   3804021
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb2813
- NMPDR:   fig|342108.5.peg.2496
- eggNOG:   COG1186
- HOGENOM:   HBG629764
- OMA:   TEMLLRM
- PhylomeDB:   Q2W3F8
- ProtClustDB:   PRK00578
- BioCyc:   MMAG342108:AMB2813-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00094
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374
- PANTHER:   PTHR11075:SF6
- SMART:   SM00937
- TIGRFAMs:   TIGR00020

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41729; Mature: 41729

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAEIEALAQDIRRSAALLKRHLNWDEALMRLDELNASAENPDLWNDAGAAQKIMRERNE
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
LDSAIQGCRALERELADLAELIELGEMEGDQTVIDDAEEQVRALKERAAKMELETLLSGE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCC
ADHNDCYMEINAGAGGTESQDWAEMLLRMYTRWAEKHGYKVEWLEESAGEQAGIKSATIR
CCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCCCCCEEEEE
ILGHNAYGWLKTESGVHRLVRISPYDSAARRHTSFSSAWVYPVIDDTIDIQINESECRID
EEECCCCEEEECCCCHHHEEEECCCHHHHHHHCCCCCEEEEEEECCEEEEEECCCCCEEE
TYRASGAGGQHINKTDSAVRITHIPTGIAVACQMERSQHQNRARAWDMLRARLYEAELQK
EEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REAAAQALEDQKTDIGWGHQIRSYVLQPYQMVKDLRTNVETSDTQGVLDGDLDMFMAASL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
AARVQGQVDQG
HHHHCCCCCCC
>Mature Secondary Structure
MRAEIEALAQDIRRSAALLKRHLNWDEALMRLDELNASAENPDLWNDAGAAQKIMRERNE
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
LDSAIQGCRALERELADLAELIELGEMEGDQTVIDDAEEQVRALKERAAKMELETLLSGE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCC
ADHNDCYMEINAGAGGTESQDWAEMLLRMYTRWAEKHGYKVEWLEESAGEQAGIKSATIR
CCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCCCCCEEEEE
ILGHNAYGWLKTESGVHRLVRISPYDSAARRHTSFSSAWVYPVIDDTIDIQINESECRID
EEECCCCEEEECCCCHHHEEEECCCHHHHHHHCCCCCEEEEEEECCEEEEEECCCCCEEE
TYRASGAGGQHINKTDSAVRITHIPTGIAVACQMERSQHQNRARAWDMLRARLYEAELQK
EEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REAAAQALEDQKTDIGWGHQIRSYVLQPYQMVKDLRTNVETSDTQGVLDGDLDMFMAASL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
AARVQGQVDQG
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA