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The map label for this gene is nuoI

Identifier: 83311878

GI number: 83311878

Start: 2965349

End: 2965837

Strand: Reverse

Name: nuoI

Synonym: amb2779

Alternate gene names: 83311878

Gene position: 2965837-2965349 (Counterclockwise)

Preceding gene: 83311879

Following gene: 83311877

Centisome position: 59.71

GC content: 65.03

Gene sequence:

>489_bases
ATGTCGTTCCTCGACCGCACCGCGCGCGCCTTTCTGCTGACCGAGCTCGTCCAGGGCTTGGCGCTGACGCTGCGCTATAT
GTTCAAGCCCAAGGTGACCATCAACTACCCCTACGAAAAGGGTCCGTTGTCGGTCCGCTTCCGTGGCGAGCACGCCCTTC
GCCGTTATCCCAACGGGGAAGAGCGCTGCATCGCCTGCAAGCTCTGCGAAGCCATTTGCCCGGCCCAGGCCATCACCATC
GAGCCCGAGCCCCGGGACGACGGTTCCCGCCGGGCCCGGCGCTACGACCTCGACATGACCAAGTGCATCTATTGCGGTTT
GTGCCAGGAGGCCTGCCCGGTGGACGCCATTGTCGAGGGTCCGAACTTCGAGTACGCCACCGAGACTCGGGCGGAGCTCA
TGTACAACAAGTCCAAGTTGCTCGCCAACGGCGACCGCTGGGAGGCCGAGCTGGCCTTCCGCATCGCCGCCGACGCGCCT
TACCGGTGA

Upstream 100 bases:

>100_bases
GAAGGTGTTCCTGCCGTTCTCGCTGTTCTGGCTGGTGCTGACCGCAAGCGTTCTGACCGCTTTCGGCTGGCTGCCGAACC
AGGGTTAAGGAGAGAAAGCG

Downstream 100 bases:

>100_bases
CGGGAGACCATCCATGTTCACGGCTCTGATCTTCTACATGTTCGCCGCAATCGCGGTGGCTTCCGGCGTGATGGTGATCT
CATCGCGCAACCCGGTGCAT

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MSFLDRTARAFLLTELVQGLALTLRYMFKPKVTINYPYEKGPLSVRFRGEHALRRYPNGEERCIACKLCEAICPAQAITI
EPEPRDDGSRRARRYDLDMTKCIYCGLCQEACPVDAIVEGPNFEYATETRAELMYNKSKLLANGDRWEAELAFRIAADAP
YR

Sequences:

>Translated_162_residues
MSFLDRTARAFLLTELVQGLALTLRYMFKPKVTINYPYEKGPLSVRFRGEHALRRYPNGEERCIACKLCEAICPAQAITI
EPEPRDDGSRRARRYDLDMTKCIYCGLCQEACPVDAIVEGPNFEYATETRAELMYNKSKLLANGDRWEAELAFRIAADAP
YR
>Mature_161_residues
SFLDRTARAFLLTELVQGLALTLRYMFKPKVTINYPYEKGPLSVRFRGEHALRRYPNGEERCIACKLCEAICPAQAITIE
PEPRDDGSRRARRYDLDMTKCIYCGLCQEACPVDAIVEGPNFEYATETRAELMYNKSKLLANGDRWEAELAFRIAADAPY
R

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=161, Percent_Identity=75.7763975155279, Blast_Score=259, Evalue=1e-69,
Organism=Escherichia coli, GI1788617, Length=139, Percent_Identity=38.1294964028777, Blast_Score=100, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17555194, Length=159, Percent_Identity=71.6981132075472, Blast_Score=241, Evalue=1e-64,
Organism=Drosophila melanogaster, GI17864306, Length=158, Percent_Identity=72.7848101265823, Blast_Score=249, Evalue=8e-67,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOI_MAGSA (Q2W3J2)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_422142.1
- HSSP:   Q9I6D2
- ProteinModelPortal:   Q2W3J2
- SMR:   Q2W3J2
- STRING:   Q2W3J2
- GeneID:   3803869
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb2779
- NMPDR:   fig|342108.5.peg.2466
- eggNOG:   COG1143
- HOGENOM:   HBG724359
- OMA:   NYPYEKG
- PhylomeDB:   Q2W3J2
- ProtClustDB:   PRK05888
- BioCyc:   MMAG342108:AMB2779-MONOMER
- HAMAP:   MF_01351
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR01971

Pfam domain/function: PF00037 Fer4

EC number: =1.6.99.5

Molecular weight: Translated: 18555; Mature: 18423

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
5.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFLDRTARAFLLTELVQGLALTLRYMFKPKVTINYPYEKGPLSVRFRGEHALRRYPNGE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCHHHHCCCCCC
ERCIACKLCEAICPAQAITIEPEPRDDGSRRARRYDLDMTKCIYCGLCQEACPVDAIVEG
HHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEC
PNFEYATETRAELMYNKSKLLANGDRWEAELAFRIAADAPYR
CCCCCCHHHHHHHHHCCHHHEECCCCCEEEEEEEEECCCCCC
>Mature Secondary Structure 
SFLDRTARAFLLTELVQGLALTLRYMFKPKVTINYPYEKGPLSVRFRGEHALRRYPNGE
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCHHHHCCCCCC
ERCIACKLCEAICPAQAITIEPEPRDDGSRRARRYDLDMTKCIYCGLCQEACPVDAIVEG
HHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEC
PNFEYATETRAELMYNKSKLLANGDRWEAELAFRIAADAPYR
CCCCCCHHHHHHHHHCCHHHEECCCCCEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA