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The map label for this gene is bioB

Identifier: 83311856

GI number: 83311856

Start: 2943285

End: 2944265

Strand: Reverse

Name: bioB

Synonym: amb2757

Alternate gene names: 83311856

Gene position: 2944265-2943285 (Counterclockwise)

Preceding gene: 83311857

Following gene: 83311855

Centisome position: 59.27

GC content: 65.34

Gene sequence:

>981_bases
GTGACGGCTCAGGCAGCTTTGGCGACAGCGGCGAATGACGGCGAACTCCGGCACGACTGGAGCGCCGAGGAGATCGAGGC
GCTGTTCGCGCTTCCCTTCAACGATCTGATGTTCGAGGCCCAGAAGGTCCACCGCGCCCATTTCGACGCCAACCGCGTCC
AGGTGTCGCGGCTGATCTCCATCAAGACCGGCTCGTGCCCCGAGGATTGCTCCTATTGCCCCCAAAGCGCCCATTACGCC
ACGGGCCTGGAAAAAGAAAAGCTCCTGGCCGTGGAGGAGGTGGTCGAATCCGCCCGTCAGGCCAAGGAGGAGGGCGCCTC
GCGCTTTTGCATGGGCGCCGCCTGGCGCGGCCCCAAGGGTGACGATTTCGAGGTGGCGGTGGCCATGATCGAGGGGGTCA
AGGCGCTGGGCATGGAAACCTGCGCCACCTTTGGCCTGCTGGACAAGTGGCAGGCCCAGCGCCTGAAGGAAGCGGGCCTG
GACTATTACAACCACAACATCGACACCAGCCCCGAGCACTATTCCGAGGTCATCACCACCCGGACCTTCCAGGATCGCCT
GGATACCCTGGACGTGGTGCGCGACGCCGGGCTGCATGTCTGTTCGGGCGGTATCGTCGGCCTGGGCGAGACCCGTACCG
ACCGGGCCCGCATGATCCAGACCCTGGCCAACATGCCCAAGCACCCCGACAGCGTGCCGATCAACCTGCTGATCCCCATC
CAGGGCACGCCGCTGGCCGATGCCGAGCGTCCCGATTCCTTCGACTTCATCCGCACCATCGCCGTGGCGCGCATCACCAT
GCCGAAATCCTTCGTCCGCCTGTCGGCGGGCCGCGAAGGCATGACCGAGGAGATGCAGGCCCTGTGCTTCCTGGCCGGCG
CCAATTCGGTATTCTGCGGCCAGAAGCTGCTGACCGCCAAGAACGCCGCGCCGGGCAAGGACAAGAGCCTGTTCGGCAAG
CTGGGCCTTCAGCCCATGTGA

Upstream 100 bases:

>100_bases
TGGCGCAGGATGACCTGGACGGGTTTTCGCTCCTGTTGCAAAGGGCGCATGGGGAGGACTACATTGCGGCCCCCGCGAGG
CATCAAACGAGGACAATGCG

Downstream 100 bases:

>100_bases
CGAACGGGGCGGAGGCGCAGGCTTCCGCCCGCCTTCCCGATTTACGGAGATGGCGTTTCGACATCCAACCGATATCGCTT
TTGGCTGATTTATCGGCTGT

Product: biotin synthase and related enzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MTAQAALATAANDGELRHDWSAEEIEALFALPFNDLMFEAQKVHRAHFDANRVQVSRLISIKTGSCPEDCSYCPQSAHYA
TGLEKEKLLAVEEVVESARQAKEEGASRFCMGAAWRGPKGDDFEVAVAMIEGVKALGMETCATFGLLDKWQAQRLKEAGL
DYYNHNIDTSPEHYSEVITTRTFQDRLDTLDVVRDAGLHVCSGGIVGLGETRTDRARMIQTLANMPKHPDSVPINLLIPI
QGTPLADAERPDSFDFIRTIAVARITMPKSFVRLSAGREGMTEEMQALCFLAGANSVFCGQKLLTAKNAAPGKDKSLFGK
LGLQPM

Sequences:

>Translated_326_residues
MTAQAALATAANDGELRHDWSAEEIEALFALPFNDLMFEAQKVHRAHFDANRVQVSRLISIKTGSCPEDCSYCPQSAHYA
TGLEKEKLLAVEEVVESARQAKEEGASRFCMGAAWRGPKGDDFEVAVAMIEGVKALGMETCATFGLLDKWQAQRLKEAGL
DYYNHNIDTSPEHYSEVITTRTFQDRLDTLDVVRDAGLHVCSGGIVGLGETRTDRARMIQTLANMPKHPDSVPINLLIPI
QGTPLADAERPDSFDFIRTIAVARITMPKSFVRLSAGREGMTEEMQALCFLAGANSVFCGQKLLTAKNAAPGKDKSLFGK
LGLQPM
>Mature_325_residues
TAQAALATAANDGELRHDWSAEEIEALFALPFNDLMFEAQKVHRAHFDANRVQVSRLISIKTGSCPEDCSYCPQSAHYAT
GLEKEKLLAVEEVVESARQAKEEGASRFCMGAAWRGPKGDDFEVAVAMIEGVKALGMETCATFGLLDKWQAQRLKEAGLD
YYNHNIDTSPEHYSEVITTRTFQDRLDTLDVVRDAGLHVCSGGIVGLGETRTDRARMIQTLANMPKHPDSVPINLLIPIQ
GTPLADAERPDSFDFIRTIAVARITMPKSFVRLSAGREGMTEEMQALCFLAGANSVFCGQKLLTAKNAAPGKDKSLFGKL
GLQPM

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=309, Percent_Identity=62.7831715210356, Blast_Score=419, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6321725, Length=329, Percent_Identity=46.8085106382979, Blast_Score=318, Evalue=8e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_MAGSA (Q2W3L4)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_422120.1
- ProteinModelPortal:   Q2W3L4
- SMR:   Q2W3L4
- STRING:   Q2W3L4
- GeneID:   3803808
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb2757
- NMPDR:   fig|342108.5.peg.2445
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   Q2W3L4
- BioCyc:   MMAG342108:AMB2757-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 35667; Mature: 35536

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQAALATAANDGELRHDWSAEEIEALFALPFNDLMFEAQKVHRAHFDANRVQVSRLIS
CCCHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHEEE
IKTGSCPEDCSYCPQSAHYATGLEKEKLLAVEEVVESARQAKEEGASRFCMGAAWRGPKG
ECCCCCCHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DDFEVAVAMIEGVKALGMETCATFGLLDKWQAQRLKEAGLDYYNHNIDTSPEHYSEVITT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHH
RTFQDRLDTLDVVRDAGLHVCSGGIVGLGETRTDRARMIQTLANMPKHPDSVPINLLIPI
HHHHHHHHHHHHHHHCCCEEECCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEE
QGTPLADAERPDSFDFIRTIAVARITMPKSFVRLSAGREGMTEEMQALCFLAGANSVFCG
CCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHC
QKLLTAKNAAPGKDKSLFGKLGLQPM
HHHHHHCCCCCCCCHHHHHHCCCCCC
>Mature Secondary Structure 
TAQAALATAANDGELRHDWSAEEIEALFALPFNDLMFEAQKVHRAHFDANRVQVSRLIS
CCHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHEEE
IKTGSCPEDCSYCPQSAHYATGLEKEKLLAVEEVVESARQAKEEGASRFCMGAAWRGPKG
ECCCCCCHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DDFEVAVAMIEGVKALGMETCATFGLLDKWQAQRLKEAGLDYYNHNIDTSPEHYSEVITT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHH
RTFQDRLDTLDVVRDAGLHVCSGGIVGLGETRTDRARMIQTLANMPKHPDSVPINLLIPI
HHHHHHHHHHHHHHHCCCEEECCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEE
QGTPLADAERPDSFDFIRTIAVARITMPKSFVRLSAGREGMTEEMQALCFLAGANSVFCG
CCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHC
QKLLTAKNAAPGKDKSLFGKLGLQPM
HHHHHHCCCCCCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA