Click here to switch to the map view.

The map label for this gene is thiG

Identifier: 83311801

GI number: 83311801

Start: 2894398

End: 2895372

Strand: Reverse

Name: thiG

Synonym: amb2702

Alternate gene names: 83311801

Gene position: 2895372-2894398 (Counterclockwise)

Preceding gene: 83311803

Following gene: 83311800

Centisome position: 58.29

GC content: 64.0

Gene sequence:

>975_bases
ATGAAGCTGGTGATCAATGGCGAGGAGCGGAACTTCTCCGCCTCCATGACCGTCGAGGCCCTGTTGGGCGAATTGGGCGT
GGATTCCCGCAAGGTGGCGGTGGAGCGCAATCTGGAGATCGTTCCCAAATCCTCATACGCCCAGGTGGCGGTGAATGACG
GCGACAAGCTGGAAATCGTCGCCTTTATCGGTGGCGGCTCGGACCAGGCCGATTCCTTCACCGTGGCCGGCAAGACCTTC
AATTCCCGCCTGCTGGTGGGCACCGGCAAGTACAAGGATTTCGAGGAAACCGCCCTGGCCATCGAGGCGTCGGGCGCCGA
GATCGTCACCGTGGCGGTGCGCCGGGTCAACCTCTCGGACCCCTCCAAGCCCATGCTGGTGGATTACGTCAGCCCCAAGA
AATACACTTTCCTGCCCAACACGGCCGGCTGCTACACCGCCGACGATTCGGTGCGCACCCTGCGTCTGGCGCGCGAGGCG
GGCGGCTGGAACCTGGTCAAGTTGGAAGTGCTGGGCGACCAGACCACCCTTTACCCCAATATGCCCGAGACCCTGAAGGC
CGCCGAGGCGCTGATCAAGGACGGCTTCGAGGTCATGGTCTACTGTTCCGACGATCCCATCCAGGCCAAGATGCTGGAAG
ACATGGGCTGCGTCGCCATCATGCCCCTGGGCTCGCTGATCGGCTCGGGCCTGGGCATTCTCAACCCCACCACCATCCGC
ATCATTAAGGATACCGTGAAGGTTCCGGTGCTGGTTGACGCCGGCGTGGGCACCGCCTCGGACGCGGCCCTGGCCATGGA
ACTGGGCTGTGACGGCGTGCTGATGAACACCGCCATCGCCCATGCCAAGGATCCCATCCGCATGGCCCGCGCCATGAAGC
TGGCCATCGAGGCCGGGCGGCTGTCCTATCTGGCCGGGCGCATGCCGAAGAAGAGCTATGCCGACCCGTCCTCGCCCACC
TCCGGCCTGATCTGA

Upstream 100 bases:

>100_bases
CTCTCGCCGGGGGATTGCCTCGCGGCCGGCTCGGTGCCATGTTTCAGGCTTGAATTGGGAGGGGGCTTGGAAGACCCCTC
AGGCAGCAGGGACATCATTC

Downstream 100 bases:

>100_bases
GGAGATTGTTGCCATGAATATGGATGCCTTCCTCGACAACGTGATGGTTTACGGCTTCTGGATCGGCCTGGGCTTCGGCG
TGTCCTGGCTGATCCGCAAG

Product: bifunctional sulfur carrier protein/thiazole synthase protein

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKLVINGEERNFSASMTVEALLGELGVDSRKVAVERNLEIVPKSSYAQVAVNDGDKLEIVAFIGGGSDQADSFTVAGKTF
NSRLLVGTGKYKDFEETALAIEASGAEIVTVAVRRVNLSDPSKPMLVDYVSPKKYTFLPNTAGCYTADDSVRTLRLAREA
GGWNLVKLEVLGDQTTLYPNMPETLKAAEALIKDGFEVMVYCSDDPIQAKMLEDMGCVAIMPLGSLIGSGLGILNPTTIR
IIKDTVKVPVLVDAGVGTASDAALAMELGCDGVLMNTAIAHAKDPIRMARAMKLAIEAGRLSYLAGRMPKKSYADPSSPT
SGLI

Sequences:

>Translated_324_residues
MKLVINGEERNFSASMTVEALLGELGVDSRKVAVERNLEIVPKSSYAQVAVNDGDKLEIVAFIGGGSDQADSFTVAGKTF
NSRLLVGTGKYKDFEETALAIEASGAEIVTVAVRRVNLSDPSKPMLVDYVSPKKYTFLPNTAGCYTADDSVRTLRLAREA
GGWNLVKLEVLGDQTTLYPNMPETLKAAEALIKDGFEVMVYCSDDPIQAKMLEDMGCVAIMPLGSLIGSGLGILNPTTIR
IIKDTVKVPVLVDAGVGTASDAALAMELGCDGVLMNTAIAHAKDPIRMARAMKLAIEAGRLSYLAGRMPKKSYADPSSPT
SGLI
>Mature_324_residues
MKLVINGEERNFSASMTVEALLGELGVDSRKVAVERNLEIVPKSSYAQVAVNDGDKLEIVAFIGGGSDQADSFTVAGKTF
NSRLLVGTGKYKDFEETALAIEASGAEIVTVAVRRVNLSDPSKPMLVDYVSPKKYTFLPNTAGCYTADDSVRTLRLAREA
GGWNLVKLEVLGDQTTLYPNMPETLKAAEALIKDGFEVMVYCSDDPIQAKMLEDMGCVAIMPLGSLIGSGLGILNPTTIR
IIKDTVKVPVLVDAGVGTASDAALAMELGCDGVLMNTAIAHAKDPIRMARAMKLAIEAGRLSYLAGRMPKKSYADPSSPT
SGLI

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=250, Percent_Identity=45.2, Blast_Score=214, Evalue=7e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_MAGSA (Q2W3R9)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_422065.1
- ProteinModelPortal:   Q2W3R9
- STRING:   Q2W3R9
- GeneID:   3802799
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb2702
- NMPDR:   fig|342108.5.peg.2398
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- PhylomeDB:   Q2W3R9
- ProtClustDB:   PRK11840
- BioCyc:   MMAG342108:AMB2702-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR012675
- InterPro:   IPR016155
- InterPro:   IPR010035
- InterPro:   IPR008867
- InterPro:   IPR003749
- Gene3D:   G3DSA:3.20.20.70
- Gene3D:   G3DSA:3.10.20.30
- TIGRFAMs:   TIGR01683

Pfam domain/function: PF05690 ThiG; PF02597 ThiS; SSF54285 Mo_synth/thiamin_syn_S_b-grasp; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 34442; Mature: 34442

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: ACT_SITE 167-167 BINDING 228-228

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVINGEERNFSASMTVEALLGELGVDSRKVAVERNLEIVPKSSYAQVAVNDGDKLEIV
CEEEEECCCCCCCHHEEHHHHHHHHCCCCEEEEEECCCEEEECCCCEEEEECCCCEEEEE
AFIGGGSDQADSFTVAGKTFNSRLLVGTGKYKDFEETALAIEASGAEIVTVAVRRVNLSD
EEEECCCCCCCEEEEECCCCCCEEEEECCCCCCCCCEEEEEECCCCEEEEEEEEEECCCC
PSKPMLVDYVSPKKYTFLPNTAGCYTADDSVRTLRLAREAGGWNLVKLEVLGDQTTLYPN
CCCCEEEEEECCCEEEECCCCCCCEECCCCHHEEEEHHHCCCCEEEEEEEECCCEEECCC
MPETLKAAEALIKDGFEVMVYCSDDPIQAKMLEDMGCVAIMPLGSLIGSGLGILNPTTIR
CCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCEEEEEHHHHHHCCCCCCCCCEEE
IIKDTVKVPVLVDAGVGTASDAALAMELGCDGVLMNTAIAHAKDPIRMARAMKLAIEAGR
EEECCCEEEEEEECCCCCCCCCEEEEEECCCEEEEHHHHHHCCCHHHHHHHHHHHHHCCH
LSYLAGRMPKKSYADPSSPTSGLI
HHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKLVINGEERNFSASMTVEALLGELGVDSRKVAVERNLEIVPKSSYAQVAVNDGDKLEIV
CEEEEECCCCCCCHHEEHHHHHHHHCCCCEEEEEECCCEEEECCCCEEEEECCCCEEEEE
AFIGGGSDQADSFTVAGKTFNSRLLVGTGKYKDFEETALAIEASGAEIVTVAVRRVNLSD
EEEECCCCCCCEEEEECCCCCCEEEEECCCCCCCCCEEEEEECCCCEEEEEEEEEECCCC
PSKPMLVDYVSPKKYTFLPNTAGCYTADDSVRTLRLAREAGGWNLVKLEVLGDQTTLYPN
CCCCEEEEEECCCEEEECCCCCCCEECCCCHHEEEEHHHCCCCEEEEEEEECCCEEECCC
MPETLKAAEALIKDGFEVMVYCSDDPIQAKMLEDMGCVAIMPLGSLIGSGLGILNPTTIR
CCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCEEEEEHHHHHHCCCCCCCCCEEE
IIKDTVKVPVLVDAGVGTASDAALAMELGCDGVLMNTAIAHAKDPIRMARAMKLAIEAGR
EEECCCEEEEEEECCCCCCCCCEEEEEECCCEEEEHHHHHHCCCHHHHHHHHHHHHHCCH
LSYLAGRMPKKSYADPSSPTSGLI
HHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA