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The map label for this gene is potH [H]

Identifier: 83311722

GI number: 83311722

Start: 2808993

End: 2809886

Strand: Reverse

Name: potH [H]

Synonym: amb2623

Alternate gene names: 83311722

Gene position: 2809886-2808993 (Counterclockwise)

Preceding gene: 83311723

Following gene: 83311721

Centisome position: 56.57

GC content: 64.65

Gene sequence:

>894_bases
ATGAAGACCCTGCTGGAGGGCAGGGGGGGCCGATTCCTGGTGGGGCTGGCGCCCTATGCCTGGCTGCTGCTGTTCTTCCT
GGTGCCTTTCCTGATCGTCCTCAAGATCAGCCTGTCCGAGCCGCAGATGGGCATTCCGCCCTATATGCCGCTGATCGACT
GGGCCGAGGGCGCTTTCAAGGGAACGCTGGCCGGCTACAACACGCTGCTGGGCGACAATCTCTATCTGCTGGCCTATCTC
GGCTCGCTGAAACTGGCGGGGATCTCGACCCTGGGCTGCCTGCTGCTGGGCTATCCCGCCGCCTATGCCATCGCCCGGGC
GCCGCAAAGCCGGCGTCAGGCGCTGCTGATGCTGGTCGCCTTGCCGTTCTGGACCTCGTTCCTGATCCGCGTCTATGCCT
GGATCGGCATCCTGAAGAACGAAGGCCTGCTCAACATGGCGCTGCAGGCCTTGGGGCTGATTTCCGAGCCGCTGCAGATT
CTCGAGACCTCGACGGCGGTGGTGATCGGCATGGTCTATTCCTACCTGCCGTTCATGATCCTGCCGCTCTACGCCACCTT
GGAGAAGATGGACCTGTCGCTGCTGGAGGCTGCCGCCGATCTGGGGTGCCGGCCGTTCAAGGCGTTTCTGGCCGTCACCT
TGCCGCTGTCCATGCCCGGCGTGATCGCCGGCTGCATGCTGGTGTTCATCCCGGCTGTGGGCGAGTTCGTCATTCCCGAC
CTGCTGGGCGGCCCCGACACGCTGGTGATCGGCAAGGTGCTGTGGGCCGAATTCTTCGACAACCGCGACTGGCCGACGGC
TTCGGCGGTGGCGGTGGCCATGCTGGTGCTCCTGGTGGTGCCCATCATGGTGTTCCAGCACTTCGAGGCCAAGCGGCAGG
AGCGCGGGGAATGA

Upstream 100 bases:

>100_bases
CGCTGCGCACCAACCTGCACCACGGCGAGGAAAGCCGGCTGACCTGGGAGGACCCGGTGTTCCTGGCCTGGCACCCGGCC
GATTCCCTGGTGCTGACCAA

Downstream 100 bases:

>100_bases
AGAATCGCGGCTTTGCCCTTTATACCGCCCTGGCGCTGGTCTACGCCTTTCTCTACCTGCCGATCGCACTGCTGGTGATC
TATTCCTTCAACGCCTCCAA

Product: ABC-type spermidine/putrescine transport system

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MKTLLEGRGGRFLVGLAPYAWLLLFFLVPFLIVLKISLSEPQMGIPPYMPLIDWAEGAFKGTLAGYNTLLGDNLYLLAYL
GSLKLAGISTLGCLLLGYPAAYAIARAPQSRRQALLMLVALPFWTSFLIRVYAWIGILKNEGLLNMALQALGLISEPLQI
LETSTAVVIGMVYSYLPFMILPLYATLEKMDLSLLEAAADLGCRPFKAFLAVTLPLSMPGVIAGCMLVFIPAVGEFVIPD
LLGGPDTLVIGKVLWAEFFDNRDWPTASAVAVAMLVLLVVPIMVFQHFEAKRQERGE

Sequences:

>Translated_297_residues
MKTLLEGRGGRFLVGLAPYAWLLLFFLVPFLIVLKISLSEPQMGIPPYMPLIDWAEGAFKGTLAGYNTLLGDNLYLLAYL
GSLKLAGISTLGCLLLGYPAAYAIARAPQSRRQALLMLVALPFWTSFLIRVYAWIGILKNEGLLNMALQALGLISEPLQI
LETSTAVVIGMVYSYLPFMILPLYATLEKMDLSLLEAAADLGCRPFKAFLAVTLPLSMPGVIAGCMLVFIPAVGEFVIPD
LLGGPDTLVIGKVLWAEFFDNRDWPTASAVAVAMLVLLVVPIMVFQHFEAKRQERGE
>Mature_297_residues
MKTLLEGRGGRFLVGLAPYAWLLLFFLVPFLIVLKISLSEPQMGIPPYMPLIDWAEGAFKGTLAGYNTLLGDNLYLLAYL
GSLKLAGISTLGCLLLGYPAAYAIARAPQSRRQALLMLVALPFWTSFLIRVYAWIGILKNEGLLNMALQALGLISEPLQI
LETSTAVVIGMVYSYLPFMILPLYATLEKMDLSLLEAAADLGCRPFKAFLAVTLPLSMPGVIAGCMLVFIPAVGEFVIPD
LLGGPDTLVIGKVLWAEFFDNRDWPTASAVAVAMLVLLVVPIMVFQHFEAKRQERGE

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=289, Percent_Identity=59.5155709342561, Blast_Score=309, Evalue=1e-85,
Organism=Escherichia coli, GI1787369, Length=272, Percent_Identity=41.5441176470588, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1787713, Length=213, Percent_Identity=34.7417840375587, Blast_Score=113, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32457; Mature: 32457

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLLEGRGGRFLVGLAPYAWLLLFFLVPFLIVLKISLSEPQMGIPPYMPLIDWAEGAFK
CCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCHHHH
GTLAGYNTLLGDNLYLLAYLGSLKLAGISTLGCLLLGYPAAYAIARAPQSRRQALLMLVA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LPFWTSFLIRVYAWIGILKNEGLLNMALQALGLISEPLQILETSTAVVIGMVYSYLPFMI
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPLYATLEKMDLSLLEAAADLGCRPFKAFLAVTLPLSMPGVIAGCMLVFIPAVGEFVIPD
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LLGGPDTLVIGKVLWAEFFDNRDWPTASAVAVAMLVLLVVPIMVFQHFEAKRQERGE
HCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTLLEGRGGRFLVGLAPYAWLLLFFLVPFLIVLKISLSEPQMGIPPYMPLIDWAEGAFK
CCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCHHHH
GTLAGYNTLLGDNLYLLAYLGSLKLAGISTLGCLLLGYPAAYAIARAPQSRRQALLMLVA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LPFWTSFLIRVYAWIGILKNEGLLNMALQALGLISEPLQILETSTAVVIGMVYSYLPFMI
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPLYATLEKMDLSLLEAAADLGCRPFKAFLAVTLPLSMPGVIAGCMLVFIPAVGEFVIPD
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LLGGPDTLVIGKVLWAEFFDNRDWPTASAVAVAMLVLLVVPIMVFQHFEAKRQERGE
HCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]