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The map label for this gene is potI [H]

Identifier: 83311721

GI number: 83311721

Start: 2808190

End: 2808996

Strand: Reverse

Name: potI [H]

Synonym: amb2622

Alternate gene names: 83311721

Gene position: 2808996-2808190 (Counterclockwise)

Preceding gene: 83311722

Following gene: 83311720

Centisome position: 56.55

GC content: 65.92

Gene sequence:

>807_bases
ATGAAGAATCGCGGCTTTGCCCTTTATACCGCCCTGGCGCTGGTCTACGCCTTTCTCTACCTGCCGATCGCACTGCTGGT
GATCTATTCCTTCAACGCCTCCAAGCTGGTGACGGTGTGGGGCGGTTTTTCCACCAAGTGGTATGGCGAGCTGCTCCATG
ATGCCCAGGTGCTGGATGCCGCCTGGCTGTCGCTGAAGATCGCCTTCCTCAACGCCTGCCTCGCCACCGCGCTGGGGACC
ATGGCGGCGGTGGTGCTGGTGCGGTTCCGGCGCTTTTCCGGCCGCGCCCTGTTCACCGGCATGGTCTCGGCGCCGCTGGT
GATGCCGGAGATCATCACCGGCCTTGCTCTGCTGCTGCTGTTCGTCGGCATGGAGAATCTGCTGGGCTGGCCCGACGGGC
GGTCCATGACCACCATCGTCATCGCCCATGTCACGTTCAGCCTGTCCTTCGTCACCGTGGTGGTGCGCTCGCGCCTCGCC
CAGATGGACCTGTCGCTGGAGGAGGCGGCCATGGACCTGGGCGCCCGGCCGCTCACCGTCTTTTTCTCCATCACCTTGCC
GATCATCGCCCCAGCCCTGGCGGCGGGATGGCTGCTGGCCTTCACGCTGTCGGTGGACGACGTGGTGATCTCCGCCTTCG
TCTCGGGTCCCGGCGCCACGCCGTTGCCCATCGTCATCTTCTCCAAGGTGCGCCTGGGCGTCAGTCCGGAGATCAACGCC
CTGGCCACCATCGTCGTCGCGCTGGTGGGCGTCGCCATCGCCGTGGCGGGCCGGGTGATGATGACCAGGGAGAAGCAGGG
CGGGTGA

Upstream 100 bases:

>100_bases
GGCCGACGGCTTCGGCGGTGGCGGTGGCCATGCTGGTGCTCCTGGTGGTGCCCATCATGGTGTTCCAGCACTTCGAGGCC
AAGCGGCAGGAGCGCGGGGA

Downstream 100 bases:

>100_bases
GCCTTTTTCTCGTCCAGACGGCGCTGATCATCGGCTTGCCTTATGCCGTGTGGCGGCTGGGGCTGATCGGCCGCCTGATG
CCGCTGGCCGCCGTGCAGAT

Product: ABC-type spermidine/putrescine transport system

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKNRGFALYTALALVYAFLYLPIALLVIYSFNASKLVTVWGGFSTKWYGELLHDAQVLDAAWLSLKIAFLNACLATALGT
MAAVVLVRFRRFSGRALFTGMVSAPLVMPEIITGLALLLLFVGMENLLGWPDGRSMTTIVIAHVTFSLSFVTVVVRSRLA
QMDLSLEEAAMDLGARPLTVFFSITLPIIAPALAAGWLLAFTLSVDDVVISAFVSGPGATPLPIVIFSKVRLGVSPEINA
LATIVVALVGVAIAVAGRVMMTREKQGG

Sequences:

>Translated_268_residues
MKNRGFALYTALALVYAFLYLPIALLVIYSFNASKLVTVWGGFSTKWYGELLHDAQVLDAAWLSLKIAFLNACLATALGT
MAAVVLVRFRRFSGRALFTGMVSAPLVMPEIITGLALLLLFVGMENLLGWPDGRSMTTIVIAHVTFSLSFVTVVVRSRLA
QMDLSLEEAAMDLGARPLTVFFSITLPIIAPALAAGWLLAFTLSVDDVVISAFVSGPGATPLPIVIFSKVRLGVSPEINA
LATIVVALVGVAIAVAGRVMMTREKQGG
>Mature_268_residues
MKNRGFALYTALALVYAFLYLPIALLVIYSFNASKLVTVWGGFSTKWYGELLHDAQVLDAAWLSLKIAFLNACLATALGT
MAAVVLVRFRRFSGRALFTGMVSAPLVMPEIITGLALLLLFVGMENLLGWPDGRSMTTIVIAHVTFSLSFVTVVVRSRLA
QMDLSLEEAAMDLGARPLTVFFSITLPIIAPALAAGWLLAFTLSVDDVVISAFVSGPGATPLPIVIFSKVRLGVSPEINA
LATIVVALVGVAIAVAGRVMMTREKQGG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=255, Percent_Identity=60.7843137254902, Blast_Score=277, Evalue=5e-76,
Organism=Escherichia coli, GI1787368, Length=255, Percent_Identity=43.5294117647059, Blast_Score=187, Evalue=5e-49,
Organism=Escherichia coli, GI1787714, Length=254, Percent_Identity=33.8582677165354, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788764, Length=208, Percent_Identity=29.8076923076923, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 28608; Mature: 28608

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNRGFALYTALALVYAFLYLPIALLVIYSFNASKLVTVWGGFSTKWYGELLHDAQVLDA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
AWLSLKIAFLNACLATALGTMAAVVLVRFRRFSGRALFTGMVSAPLVMPEIITGLALLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHHHH
FVGMENLLGWPDGRSMTTIVIAHVTFSLSFVTVVVRSRLAQMDLSLEEAAMDLGARPLTV
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHH
FFSITLPIIAPALAAGWLLAFTLSVDDVVISAFVSGPGATPLPIVIFSKVRLGVSPEINA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHH
LATIVVALVGVAIAVAGRVMMTREKQGG
HHHHHHHHHHHHHHHHHHHHEECCCCCC
>Mature Secondary Structure
MKNRGFALYTALALVYAFLYLPIALLVIYSFNASKLVTVWGGFSTKWYGELLHDAQVLDA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
AWLSLKIAFLNACLATALGTMAAVVLVRFRRFSGRALFTGMVSAPLVMPEIITGLALLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHHHH
FVGMENLLGWPDGRSMTTIVIAHVTFSLSFVTVVVRSRLAQMDLSLEEAAMDLGARPLTV
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHH
FFSITLPIIAPALAAGWLLAFTLSVDDVVISAFVSGPGATPLPIVIFSKVRLGVSPEINA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHH
LATIVVALVGVAIAVAGRVMMTREKQGG
HHHHHHHHHHHHHHHHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]