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The map label for this gene is paaF [C]

Identifier: 83311690

GI number: 83311690

Start: 2776956

End: 2777723

Strand: Reverse

Name: paaF [C]

Synonym: amb2591

Alternate gene names: 83311690

Gene position: 2777723-2776956 (Counterclockwise)

Preceding gene: 83311691

Following gene: 83311689

Centisome position: 55.92

GC content: 68.23

Gene sequence:

>768_bases
ATGTCCTTCTCCGTGCTGAATACCGCCCGCCGGGGCGCCGTCTTCGTGGTGACTCTGGCCAGCCCGCCGGTCAACGCCCT
GTCGCGTGCCCTGATCAAGGATCTGCACGCGGCCATGGACATGGTGGAGGCGGACAAGACCATCCGCGTCCTGCATCTGC
GCTCCGAGCAAAAGGCCTTCTGCGCCGGGGCCGACCTGGCCGAGATGCGTGAGAATCTGGCCAATCCCGATCTGGTGGAT
GCCCAGATCGCCTTTGTCCGCGACCTCCAGAACGTCTTGAAGCGCATCGAGACCTTGGCCCTCGCCACCGTCGCCGAGGT
GGGCGGCGCGGCCATGGGCGGCGGGCTGGAACTGGCGTTGGCCTGTGATTTCCGCATGGCCGCCAACGAGGCCAAGCTGG
CCCTGCCCGAGGTCAATCTCGGCCTGATCCCGGGGGCGGGGGGGACCCAGCGCCTGACCCGGCTGTGCGGCCCGGCCATC
GCCAAGCGCCTGATTCTGGGGGCCGAGATTCTCGACGGCCAAAGCGCCGAGGCCATGGGGATCGTCCATTGGTCGGCGCC
CCGCGCCGAACTGGCCGACAAGGCCGCCACCCTGGCCGATAGGCTCGCCACCTTGCCGCGTGCCGCGGTGGCGGCCTCGA
AGTCCTGCATCGAAGCCTCTCTCGATCCGTCCCGCGACGGCTACGAGGAGGAATTGACCGCCACCCGTAACCTGCTGTTG
CACGAGCCCGAGACCCGTCACCGGGTCGAGGCGTTCCTGTCCAAGTAA

Upstream 100 bases:

>100_bases
ATGACACATTGACTGAACGCCTATTCAATCGCGCTTGACTTGGCGCATCGAATGAATGAACATTCAGTCATATTGAATGG
TCATCCAGTTCGAGGCGTGA

Downstream 100 bases:

>100_bases
TCCCATTCGAAGGAGCCAAGGAAATGAAGTTCGAGGATAAAGTCGCCCTGATTACCGGCGGTGCCTCCGGCATCGGTTAC
TGCACCGTCAAGTCCATGGC

Product: enoyl-CoA hydratase/carnithine racemase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSFSVLNTARRGAVFVVTLASPPVNALSRALIKDLHAAMDMVEADKTIRVLHLRSEQKAFCAGADLAEMRENLANPDLVD
AQIAFVRDLQNVLKRIETLALATVAEVGGAAMGGGLELALACDFRMAANEAKLALPEVNLGLIPGAGGTQRLTRLCGPAI
AKRLILGAEILDGQSAEAMGIVHWSAPRAELADKAATLADRLATLPRAAVAASKSCIEASLDPSRDGYEEELTATRNLLL
HEPETRHRVEAFLSK

Sequences:

>Translated_255_residues
MSFSVLNTARRGAVFVVTLASPPVNALSRALIKDLHAAMDMVEADKTIRVLHLRSEQKAFCAGADLAEMRENLANPDLVD
AQIAFVRDLQNVLKRIETLALATVAEVGGAAMGGGLELALACDFRMAANEAKLALPEVNLGLIPGAGGTQRLTRLCGPAI
AKRLILGAEILDGQSAEAMGIVHWSAPRAELADKAATLADRLATLPRAAVAASKSCIEASLDPSRDGYEEELTATRNLLL
HEPETRHRVEAFLSK
>Mature_254_residues
SFSVLNTARRGAVFVVTLASPPVNALSRALIKDLHAAMDMVEADKTIRVLHLRSEQKAFCAGADLAEMRENLANPDLVDA
QIAFVRDLQNVLKRIETLALATVAEVGGAAMGGGLELALACDFRMAANEAKLALPEVNLGLIPGAGGTQRLTRLCGPAIA
KRLILGAEILDGQSAEAMGIVHWSAPRAELADKAATLADRLATLPRAAVAASKSCIEASLDPSRDGYEEELTATRNLLLH
EPETRHRVEAFLSK

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=225, Percent_Identity=33.7777777777778, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI4502327, Length=190, Percent_Identity=43.6842105263158, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI68989263, Length=194, Percent_Identity=34.5360824742268, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI20127408, Length=236, Percent_Identity=31.3559322033898, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI157694516, Length=172, Percent_Identity=33.1395348837209, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI213417737, Length=172, Percent_Identity=33.1395348837209, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI157694520, Length=133, Percent_Identity=36.8421052631579, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI31542718, Length=235, Percent_Identity=27.2340425531915, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI70995211, Length=208, Percent_Identity=28.3653846153846, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI261878539, Length=96, Percent_Identity=42.7083333333333, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=257, Percent_Identity=33.4630350194552, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788682, Length=192, Percent_Identity=33.8541666666667, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI221142681, Length=249, Percent_Identity=31.3253012048193, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790281, Length=159, Percent_Identity=33.9622641509434, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87082183, Length=217, Percent_Identity=28.110599078341, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787660, Length=256, Percent_Identity=31.25, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788597, Length=233, Percent_Identity=26.6094420600858, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17540714, Length=206, Percent_Identity=35.9223300970874, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25145438, Length=241, Percent_Identity=32.7800829875519, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17554946, Length=238, Percent_Identity=29.8319327731092, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17508951, Length=153, Percent_Identity=40.5228758169935, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI25144276, Length=153, Percent_Identity=40.5228758169935, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17508953, Length=153, Percent_Identity=40.5228758169935, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17558304, Length=189, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI25144160, Length=169, Percent_Identity=31.3609467455621, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25144157, Length=169, Percent_Identity=31.3609467455621, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17534483, Length=219, Percent_Identity=24.6575342465753, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560910, Length=235, Percent_Identity=25.531914893617, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=214, Percent_Identity=24.7663551401869, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17536985, Length=229, Percent_Identity=23.5807860262009, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=187, Percent_Identity=28.8770053475936, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24653139, Length=210, Percent_Identity=40.4761904761905, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI20129971, Length=212, Percent_Identity=35.8490566037736, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24653477, Length=212, Percent_Identity=35.8490566037736, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI28571729, Length=156, Percent_Identity=28.2051282051282, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28571730, Length=156, Percent_Identity=28.2051282051282, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583165, Length=224, Percent_Identity=25, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27095; Mature: 26963

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSVLNTARRGAVFVVTLASPPVNALSRALIKDLHAAMDMVEADKTIRVLHLRSEQKAF
CCCCHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHH
CAGADLAEMRENLANPDLVDAQIAFVRDLQNVLKRIETLALATVAEVGGAAMGGGLELAL
HCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
ACDFRMAANEAKLALPEVNLGLIPGAGGTQRLTRLCGPAIAKRLILGAEILDGQSAEAMG
EECHHHHCCCCCEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
IVHWSAPRAELADKAATLADRLATLPRAAVAASKSCIEASLDPSRDGYEEELTATRNLLL
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
HEPETRHRVEAFLSK
CCCCHHHHHHHHHCC
>Mature Secondary Structure 
SFSVLNTARRGAVFVVTLASPPVNALSRALIKDLHAAMDMVEADKTIRVLHLRSEQKAF
CCCHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHH
CAGADLAEMRENLANPDLVDAQIAFVRDLQNVLKRIETLALATVAEVGGAAMGGGLELAL
HCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
ACDFRMAANEAKLALPEVNLGLIPGAGGTQRLTRLCGPAIAKRLILGAEILDGQSAEAMG
EECHHHHCCCCCEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
IVHWSAPRAELADKAATLADRLATLPRAAVAASKSCIEASLDPSRDGYEEELTATRNLLL
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
HEPETRHRVEAFLSK
CCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA