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The map label for this gene is lip2 [H]

Identifier: 83311691

GI number: 83311691

Start: 2777927

End: 2778874

Strand: Reverse

Name: lip2 [H]

Synonym: amb2592

Alternate gene names: 83311691

Gene position: 2778874-2777927 (Counterclockwise)

Preceding gene: 83311692

Following gene: 83311690

Centisome position: 55.95

GC content: 70.46

Gene sequence:

>948_bases
ATGCTGGAAACCGGCTTTTTCTCCCTGGGGAGCCCCGGGCAGCTGGACGCGGACATCGCCTTCGTGCTGGCCAAGGCCGC
CGAGCTGAACCTGCCCAAGCTGGCCAGCCTGGGGCCGGTGGAGGGGCGGGCGGAATTCGCCCGGCGCCTGTCGCGCACCA
ATCCTCCGGCGCCCGAGGGTGTCGAGGCCGAGGACATCTCCATCGGCGACATTCCTGCCCGGCGCTACCAGCCCCAGGGA
GGCGCCAAGGTCACGGCGCTGCTGCTTTACTTCCACGGCGGCGGATTCGTGGTCGGCGACCTGGAGACGCACGACCCCCA
CTGCCGCGCCCTGGCCGCCGAGAGCGGCTCGGTGGTGGTGGCGTTGGATTACCGCATGGGGCCGGAGCATCCCTATCCCG
CCGCCATCCAGGACGGCATTGCCGCCCTGCGCTGGGCGGCGGCGGATTATCCCGGCTGGGCTCTGGGCGTGGCCGGCGAC
AGCGCCGGCGGCACCCTGTCGGCGGTGGTGGCCCTGCATGCCCGCGATCTGGGGATCGCCCTGGCGGCCCAGTTGCTGAT
CTATCCGGCGGTGGATCAGGCGGGCGATTTTCCCTCCCGCATCCGGTTGGCCGACGGCTATCTGCTGACCCAGGCCGATA
TCGAGTGGTTCACCGAGCAGTATTTCGTTGGGCGCACCAATCCGGTGTTGGAGCCCGATGCCTCGCCCTTGCGGGCCGCT
TCCCATGCCGGGCTGGCGCCGGCCCTGGTGCTGACCTGCGGCTTCGACCCTCTGGTGGACGAGGGCGACGCCTATGCCGA
GGCGCTGAAGGGGGCGGGAGTGCCGGTGCGCCATGTGCGCCTGGAAGGCTCCATCCATGGCTGCCTGGGACTGGCGGGCT
ACGTCCAGTCGGGCAAGCAGGCCCTGGCGGAAGTCTGCCGGGCGTGGCGTTCGGTGGCTTCTTCCTAG

Upstream 100 bases:

>100_bases
TATCCGCCAGTCCGGCGTCGGCCGGGAATGGGGCGAGGCGGGGTTCGAGGAGTTCCTGGAATCCAAGACCCTGTTCGGCT
CCGAGCCGAAGGAGTAGGAC

Downstream 100 bases:

>100_bases
ATCGGGGTGTCATTCCGAGGCGAAGCCGAGGAATCTCCGGCTGGAACATTGCTGCCGATCCGGTGAAAGCATTCCAGGAT
GAGATCCCTCGCATTCGCTC

Product: esterase/lipase

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MLETGFFSLGSPGQLDADIAFVLAKAAELNLPKLASLGPVEGRAEFARRLSRTNPPAPEGVEAEDISIGDIPARRYQPQG
GAKVTALLLYFHGGGFVVGDLETHDPHCRALAAESGSVVVALDYRMGPEHPYPAAIQDGIAALRWAAADYPGWALGVAGD
SAGGTLSAVVALHARDLGIALAAQLLIYPAVDQAGDFPSRIRLADGYLLTQADIEWFTEQYFVGRTNPVLEPDASPLRAA
SHAGLAPALVLTCGFDPLVDEGDAYAEALKGAGVPVRHVRLEGSIHGCLGLAGYVQSGKQALAEVCRAWRSVASS

Sequences:

>Translated_315_residues
MLETGFFSLGSPGQLDADIAFVLAKAAELNLPKLASLGPVEGRAEFARRLSRTNPPAPEGVEAEDISIGDIPARRYQPQG
GAKVTALLLYFHGGGFVVGDLETHDPHCRALAAESGSVVVALDYRMGPEHPYPAAIQDGIAALRWAAADYPGWALGVAGD
SAGGTLSAVVALHARDLGIALAAQLLIYPAVDQAGDFPSRIRLADGYLLTQADIEWFTEQYFVGRTNPVLEPDASPLRAA
SHAGLAPALVLTCGFDPLVDEGDAYAEALKGAGVPVRHVRLEGSIHGCLGLAGYVQSGKQALAEVCRAWRSVASS
>Mature_315_residues
MLETGFFSLGSPGQLDADIAFVLAKAAELNLPKLASLGPVEGRAEFARRLSRTNPPAPEGVEAEDISIGDIPARRYQPQG
GAKVTALLLYFHGGGFVVGDLETHDPHCRALAAESGSVVVALDYRMGPEHPYPAAIQDGIAALRWAAADYPGWALGVAGD
SAGGTLSAVVALHARDLGIALAAQLLIYPAVDQAGDFPSRIRLADGYLLTQADIEWFTEQYFVGRTNPVLEPDASPLRAA
SHAGLAPALVLTCGFDPLVDEGDAYAEALKGAGVPVRHVRLEGSIHGCLGLAGYVQSGKQALAEVCRAWRSVASS

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=310, Percent_Identity=27.4193548387097, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI68299767, Length=309, Percent_Identity=28.1553398058252, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI21328446, Length=132, Percent_Identity=34.8484848484849, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI157041239, Length=295, Percent_Identity=27.7966101694915, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1786682, Length=293, Percent_Identity=29.0102389078498, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71996133, Length=185, Percent_Identity=28.6486486486486, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17540028, Length=314, Percent_Identity=24.8407643312102, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17567059, Length=121, Percent_Identity=36.3636363636364, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 32931; Mature: 32931

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS01173 LIPASE_GDXG_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLETGFFSLGSPGQLDADIAFVLAKAAELNLPKLASLGPVEGRAEFARRLSRTNPPAPEG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
VEAEDISIGDIPARRYQPQGGAKVTALLLYFHGGGFVVGDLETHDPHCRALAAESGSVVV
CCCCCCCCCCCCHHHCCCCCCCEEEEEEEEEECCCEEEECCCCCCCHHEEEECCCCCEEE
ALDYRMGPEHPYPAAIQDGIAALRWAAADYPGWALGVAGDSAGGTLSAVVALHARDLGIA
EEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
LAAQLLIYPAVDQAGDFPSRIRLADGYLLTQADIEWFTEQYFVGRTNPVLEPDASPLRAA
HHHHHHHHCCHHCCCCCCCCEEEECCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
SHAGLAPALVLTCGFDPLVDEGDAYAEALKGAGVPVRHVRLEGSIHGCLGLAGYVQSGKQ
HCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHH
ALAEVCRAWRSVASS
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MLETGFFSLGSPGQLDADIAFVLAKAAELNLPKLASLGPVEGRAEFARRLSRTNPPAPEG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
VEAEDISIGDIPARRYQPQGGAKVTALLLYFHGGGFVVGDLETHDPHCRALAAESGSVVV
CCCCCCCCCCCCHHHCCCCCCCEEEEEEEEEECCCEEEECCCCCCCHHEEEECCCCCEEE
ALDYRMGPEHPYPAAIQDGIAALRWAAADYPGWALGVAGDSAGGTLSAVVALHARDLGIA
EEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
LAAQLLIYPAVDQAGDFPSRIRLADGYLLTQADIEWFTEQYFVGRTNPVLEPDASPLRAA
HHHHHHHHCCHHCCCCCCCCEEEECCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
SHAGLAPALVLTCGFDPLVDEGDAYAEALKGAGVPVRHVRLEGSIHGCLGLAGYVQSGKQ
HCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHH
ALAEVCRAWRSVASS
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]