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The map label for this gene is ilvD

Identifier: 83311635

GI number: 83311635

Start: 2718670

End: 2720520

Strand: Reverse

Name: ilvD

Synonym: amb2536

Alternate gene names: 83311635

Gene position: 2720520-2718670 (Counterclockwise)

Preceding gene: 83311636

Following gene: 83311634

Centisome position: 54.77

GC content: 66.56

Gene sequence:

>1851_bases
ATGCCTGCTTATCGTTCCCGTACCTCGACCCACGGCCGCAACATGTCCGGCGCCCGCGGCCTGTGGCGCGCCACCGGCAT
GACCGACGCCGATTTCGGCAAGCCGATCATCGCCATCGCCAATTCCTTCACCCAGTTCGTGCCCGGCCACGTGCACCTCA
AGGATCTGGGCCAGATGGTGGCGCGCGAGATCGAGAAGGCGGGCGGCGTCGCCAAGGAATTCGACACCATTGCCATCGAT
GACGGCATCGCCATGGGCCATGCGGGCATGCTGTATTCGCTGCCCAGTCGCGAGCTGATCGCCGACAGCGTCGAGTACAT
GGTCAACGCCCATTGCGCCGACGCCATGGTCTGCATCTCCAATTGCGACAAGATCACCCCCGGCATGCTGATGGCCAGCT
TGCGCCTCAACATCCCGACCATCTTCATCTCGGGCGGCCCCATGGAAGCTGGCAAGGTCACCTATCAGGGCGAGACCCAC
GCCGTCGACCTTATCGATGCCATGATCAAGGGCGCTGACCAGAACGTCTCGGACGAGGAGGCCCTGGCCTTCGAGAAGGA
AAGCTGCCCCACCTGCGGCTCGTGCTCGGGCATGTTCACCGCCAATTCCATGAACTGCCTGATCGAGGCCCTGGGCCTGG
GCCTGCCCGGCAACGGCACGGTGGTCGCCACCCATGCCGACCGCAAGGAGCTGTTCCTGGAAGCCGGGCGGCGCATCGTC
GATCTGGCCCGCCGCGCCTATGAGGGCGATGACGCCTCGGTCCTGCCGCGCTCCATCGCCACCTTCCAGGCCTTCGAGAA
CGCCATGACCCTGGACATCGCCATGGGCGGCTCCACCAATACCGTGCTGCACCTGCTGGCGGCGGCGCAGGAGGCGGGCG
TGGCGTTCGGCATGAGCGACATCGACCGCCTGTCGCGCCGGGTGCCCTGCCTGTGCAAGGTGGCCCCCAACGTTCCCGAT
GTCCATATCGAGGACGTGCACCGGGCCGGCGGCATCATGGGCATCCTGGGCCAGCTGGACCGGGGCGGCCTGATCAACCG
CGAGTGCGGCACCATCCATGCCCGCACCCTGGCCGAGGGCCTGGACAGATGGGACATCTCGCGTTCCAACGACCCCAAGG
TGGTGGAGTTCTACAAGGCCGCCCCCGGCGGCGTGCGCACCACCGAGGCCTTCAGCCAGTCCAAGCGCTGGGCCGAGGTG
GACAAGGACCGCACGGCGGGCGTCATCCGCTCGGTTGACAATGCATTTTCCAAGGATGGCGGTCTGGCGGTGCTGTTCGG
CAACCTGGCGCTGGACGGATGCATCGTCAAGACGGCGGGCGTCGACGACAAGAACCTGACCTTCTCCGGCCCGGCGGTGA
TCTGTGAAAGCCAGGACGAGGCGGTGGCCAAGATCCTGGGCGGTCAGGTCAAGTCCGGCGACGTGGTCATCGTCCGCTAC
GAGGGACCGCGCGGCGGCCCCGGCATGCAGGAAATGCTTTACCCCACCAGCTACCTGAAGAGCATGGGCCTGGGCAAGGA
ATGCGCCCTGATCACCGACGGCCGCTTCTCGGGCGGCACTTCGGGGCTGTCCATCGGCCATGTCTCGCCCGAGGCGGCGG
AAGGCGGGGCCATCGGGCTGATCCAGGCGGGCGACATCATCGATATTCATATCCCGAACCGCAGTATTGCGATCCGGGTG
TCCGATGCCGAACTGGAAAAGCGCCGGCAGGCCATGCAGGCCTTGGGGCCGAAGGCCTGGAAGCCGGTGGATCGCGACCG
TCAGGTGTCGGCGGCGCTTCGGGCCTATGCCGCCATGACCACCAGCGCGGCACGCGGAGCGGTGCGCGACGTCTCGCAAC
TGGAACGCTGA

Upstream 100 bases:

>100_bases
AGCTTTCGATGCCGAGGCCGAGCGCCTGGGCATCAGTAATCCCGAGAAAGTCCGGGGCTGACCCGGCCCTCACGTTTCCG
CCAGACGAAGGTTGACTTCC

Downstream 100 bases:

>100_bases
GGAAGGACCGGCCGATGGGGGTGACGTGGAGTGAGCGGATGAGCGTCGGCGTGCCGCTGCTCGATTCCGACCATAAAACC
CTCATCGGCCTGATCAATCA

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD

Number of amino acids: Translated: 616; Mature: 615

Protein sequence:

>616_residues
MPAYRSRTSTHGRNMSGARGLWRATGMTDADFGKPIIAIANSFTQFVPGHVHLKDLGQMVAREIEKAGGVAKEFDTIAID
DGIAMGHAGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPTIFISGGPMEAGKVTYQGETH
AVDLIDAMIKGADQNVSDEEALAFEKESCPTCGSCSGMFTANSMNCLIEALGLGLPGNGTVVATHADRKELFLEAGRRIV
DLARRAYEGDDASVLPRSIATFQAFENAMTLDIAMGGSTNTVLHLLAAAQEAGVAFGMSDIDRLSRRVPCLCKVAPNVPD
VHIEDVHRAGGIMGILGQLDRGGLINRECGTIHARTLAEGLDRWDISRSNDPKVVEFYKAAPGGVRTTEAFSQSKRWAEV
DKDRTAGVIRSVDNAFSKDGGLAVLFGNLALDGCIVKTAGVDDKNLTFSGPAVICESQDEAVAKILGGQVKSGDVVIVRY
EGPRGGPGMQEMLYPTSYLKSMGLGKECALITDGRFSGGTSGLSIGHVSPEAAEGGAIGLIQAGDIIDIHIPNRSIAIRV
SDAELEKRRQAMQALGPKAWKPVDRDRQVSAALRAYAAMTTSAARGAVRDVSQLER

Sequences:

>Translated_616_residues
MPAYRSRTSTHGRNMSGARGLWRATGMTDADFGKPIIAIANSFTQFVPGHVHLKDLGQMVAREIEKAGGVAKEFDTIAID
DGIAMGHAGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPTIFISGGPMEAGKVTYQGETH
AVDLIDAMIKGADQNVSDEEALAFEKESCPTCGSCSGMFTANSMNCLIEALGLGLPGNGTVVATHADRKELFLEAGRRIV
DLARRAYEGDDASVLPRSIATFQAFENAMTLDIAMGGSTNTVLHLLAAAQEAGVAFGMSDIDRLSRRVPCLCKVAPNVPD
VHIEDVHRAGGIMGILGQLDRGGLINRECGTIHARTLAEGLDRWDISRSNDPKVVEFYKAAPGGVRTTEAFSQSKRWAEV
DKDRTAGVIRSVDNAFSKDGGLAVLFGNLALDGCIVKTAGVDDKNLTFSGPAVICESQDEAVAKILGGQVKSGDVVIVRY
EGPRGGPGMQEMLYPTSYLKSMGLGKECALITDGRFSGGTSGLSIGHVSPEAAEGGAIGLIQAGDIIDIHIPNRSIAIRV
SDAELEKRRQAMQALGPKAWKPVDRDRQVSAALRAYAAMTTSAARGAVRDVSQLER
>Mature_615_residues
PAYRSRTSTHGRNMSGARGLWRATGMTDADFGKPIIAIANSFTQFVPGHVHLKDLGQMVAREIEKAGGVAKEFDTIAIDD
GIAMGHAGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPTIFISGGPMEAGKVTYQGETHA
VDLIDAMIKGADQNVSDEEALAFEKESCPTCGSCSGMFTANSMNCLIEALGLGLPGNGTVVATHADRKELFLEAGRRIVD
LARRAYEGDDASVLPRSIATFQAFENAMTLDIAMGGSTNTVLHLLAAAQEAGVAFGMSDIDRLSRRVPCLCKVAPNVPDV
HIEDVHRAGGIMGILGQLDRGGLINRECGTIHARTLAEGLDRWDISRSNDPKVVEFYKAAPGGVRTTEAFSQSKRWAEVD
KDRTAGVIRSVDNAFSKDGGLAVLFGNLALDGCIVKTAGVDDKNLTFSGPAVICESQDEAVAKILGGQVKSGDVVIVRYE
GPRGGPGMQEMLYPTSYLKSMGLGKECALITDGRFSGGTSGLSIGHVSPEAAEGGAIGLIQAGDIIDIHIPNRSIAIRVS
DAELEKRRQAMQALGPKAWKPVDRDRQVSAALRAYAAMTTSAARGAVRDVSQLER

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family

Homologues:

Organism=Escherichia coli, GI48994964, Length=614, Percent_Identity=71.4983713355049, Blast_Score=925, Evalue=0.0,
Organism=Escherichia coli, GI1788157, Length=563, Percent_Identity=28.2415630550622, Blast_Score=189, Evalue=6e-49,
Organism=Escherichia coli, GI2367371, Length=618, Percent_Identity=28.9644012944984, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1786464, Length=527, Percent_Identity=31.1195445920304, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6322476, Length=608, Percent_Identity=35.1973684210526, Blast_Score=328, Evalue=2e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVD_MAGSA (Q2W485)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_421899.1
- STRING:   Q2W485
- GeneID:   3802369
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb2536
- NMPDR:   fig|342108.5.peg.2239
- eggNOG:   COG0129
- HOGENOM:   HBG671001
- OMA:   KVPCLSK
- PhylomeDB:   Q2W485
- ProtClustDB:   PRK12448
- BioCyc:   MMAG342108:AMB2536-MONOMER
- HAMAP:   MF_00012
- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558
- PANTHER:   PTHR21000
- TIGRFAMs:   TIGR00110

Pfam domain/function: PF00920 ILVD_EDD; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.9

Molecular weight: Translated: 65401; Mature: 65270

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00886 ILVD_EDD_1; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAYRSRTSTHGRNMSGARGLWRATGMTDADFGKPIIAIANSFTQFVPGHVHLKDLGQMV
CCCCCCCCCCCCCCCCCCCCHHEECCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHH
AREIEKAGGVAKEFDTIAIDDGIAMGHAGMLYSLPSRELIADSVEYMVNAHCADAMVCIS
HHHHHHHCCCHHHCCEEEECCCCEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
NCDKITPGMLMASLRLNIPTIFISGGPMEAGKVTYQGETHAVDLIDAMIKGADQNVSDEE
CCCCCCHHHHHHHHEECCCEEEEECCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCHH
ALAFEKESCPTCGSCSGMFTANSMNCLIEALGLGLPGNGTVVATHADRKELFLEAGRRIV
HHHCCCCCCCCCCCCCCCEECCHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHCHHHH
DLARRAYEGDDASVLPRSIATFQAFENAMTLDIAMGGSTNTVLHLLAAAQEAGVAFGMSD
HHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHCCCEECHHH
IDRLSRRVPCLCKVAPNVPDVHIEDVHRAGGIMGILGQLDRGGLINRECGTIHARTLAEG
HHHHHHCCCEEEEECCCCCCCCHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
LDRWDISRSNDPKVVEFYKAAPGGVRTTEAFSQSKRWAEVDKDRTAGVIRSVDNAFSKDG
HHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
GLAVLFGNLALDGCIVKTAGVDDKNLTFSGPAVICESQDEAVAKILGGQVKSGDVVIVRY
CEEEEEECCCCCCEEEEECCCCCCCEEECCCEEEECCCHHHHHHHHCCCCCCCCEEEEEE
EGPRGGPGMQEMLYPTSYLKSMGLGKECALITDGRFSGGTSGLSIGHVSPEAAEGGAIGL
CCCCCCCCHHHHHCCHHHHHHCCCCCCEEEEECCCCCCCCCCEEECCCCCCCCCCCEEEE
IQAGDIIDIHIPNRSIAIRVSDAELEKRRQAMQALGPKAWKPVDRDRQVSAALRAYAAMT
EECCCEEEEEECCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
TSAARGAVRDVSQLER
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PAYRSRTSTHGRNMSGARGLWRATGMTDADFGKPIIAIANSFTQFVPGHVHLKDLGQMV
CCCCCCCCCCCCCCCCCCCHHEECCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHH
AREIEKAGGVAKEFDTIAIDDGIAMGHAGMLYSLPSRELIADSVEYMVNAHCADAMVCIS
HHHHHHHCCCHHHCCEEEECCCCEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
NCDKITPGMLMASLRLNIPTIFISGGPMEAGKVTYQGETHAVDLIDAMIKGADQNVSDEE
CCCCCCHHHHHHHHEECCCEEEEECCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCHH
ALAFEKESCPTCGSCSGMFTANSMNCLIEALGLGLPGNGTVVATHADRKELFLEAGRRIV
HHHCCCCCCCCCCCCCCCEECCHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHCHHHH
DLARRAYEGDDASVLPRSIATFQAFENAMTLDIAMGGSTNTVLHLLAAAQEAGVAFGMSD
HHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHCCCEECHHH
IDRLSRRVPCLCKVAPNVPDVHIEDVHRAGGIMGILGQLDRGGLINRECGTIHARTLAEG
HHHHHHCCCEEEEECCCCCCCCHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
LDRWDISRSNDPKVVEFYKAAPGGVRTTEAFSQSKRWAEVDKDRTAGVIRSVDNAFSKDG
HHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
GLAVLFGNLALDGCIVKTAGVDDKNLTFSGPAVICESQDEAVAKILGGQVKSGDVVIVRY
CEEEEEECCCCCCEEEEECCCCCCCEEECCCEEEECCCHHHHHHHHCCCCCCCCEEEEEE
EGPRGGPGMQEMLYPTSYLKSMGLGKECALITDGRFSGGTSGLSIGHVSPEAAEGGAIGL
CCCCCCCCHHHHHCCHHHHHHCCCCCCEEEEECCCCCCCCCCEEECCCCCCCCCCCEEEE
IQAGDIIDIHIPNRSIAIRVSDAELEKRRQAMQALGPKAWKPVDRDRQVSAALRAYAAMT
EECCCEEEEEECCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
TSAARGAVRDVSQLER
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA