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The map label for this gene is pcm

Identifier: 83311618

GI number: 83311618

Start: 2701453

End: 2702097

Strand: Reverse

Name: pcm

Synonym: amb2519

Alternate gene names: 83311618

Gene position: 2702097-2701453 (Counterclockwise)

Preceding gene: 83311619

Following gene: 83311617

Centisome position: 54.4

GC content: 66.98

Gene sequence:

>645_bases
ATGAAGAAGGCGGAACCGCGCGTCATCCGCCTGCTGATGGAACTGCGGCGGATGGGTGTCGTCGATACCCGCGTCCTGTC
GGCCATCGAACGCATTCCCCGCGCCCTGTTCGTCGCCGAGCCCTTCCTTGATCAGGCCTATGAGAACACCGCCCTGCCCA
TCGGCTGCGCCCAGACCATCAGCCAGCCCCTGGTGGTCGGCCTGATGAGCCAGGCCCTCGAGGTGGGCGAGCGCATGAAG
GTCCTGGAGATCGGCACCGGCTCGGGCTATCAGGCGGCGGTGCTGGCCAAGCTGTGCCGTCGCCTTTATTCGGTGGAGCG
CCACAAACCGCTGCTGGCCGAGGCCGAGGCCCGTTTCAAGCACCTGCGTCTGCACAACATTACCTGCCGGGCCGCCGACG
GCTCTCGCGGCTGGCCGGAGCAGGCGCCCTTCGACCGGATCATGGTCACCGCCGCCGCACCCGATATCCCGCCCGCCCTG
GTGGACCAGTTGAAGCCCGACGGCATCATGGTGCTGCCCCTGGGGGATGTGGGCGGGATCGACCAGGAACTGGTGCGGAT
CACCAAGACCGATCGCGGCATCGACATCCAGCCGTTTCTGCCGGTGCGTTTCGTGCCGCTGGTGGAGGGGATACCGGAGG
AATGA

Upstream 100 bases:

>100_bases
CCGATATCGAGGCGGTGTTTCGCGGCGCCATTTCGGTGACCCCGCTGTGCTTCGATCTCACCCACCGCGACGACATGAAG
GCGCTGGAGACGGCCTTTTG

Downstream 100 bases:

>100_bases
TTGCCGCTGGCCGTCGCCGCGCTTATAGTACGGGCATGAGGCACGCGCCCCATCATCTGGTTATTCTCCTGATCCTCGCC
GCGGCGATCTCGGCGTGCAC

Product: protein-L-isoaspartate carboxylmethyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKKAEPRVIRLLMELRRMGVVDTRVLSAIERIPRALFVAEPFLDQAYENTALPIGCAQTISQPLVVGLMSQALEVGERMK
VLEIGTGSGYQAAVLAKLCRRLYSVERHKPLLAEAEARFKHLRLHNITCRAADGSRGWPEQAPFDRIMVTAAAPDIPPAL
VDQLKPDGIMVLPLGDVGGIDQELVRITKTDRGIDIQPFLPVRFVPLVEGIPEE

Sequences:

>Translated_214_residues
MKKAEPRVIRLLMELRRMGVVDTRVLSAIERIPRALFVAEPFLDQAYENTALPIGCAQTISQPLVVGLMSQALEVGERMK
VLEIGTGSGYQAAVLAKLCRRLYSVERHKPLLAEAEARFKHLRLHNITCRAADGSRGWPEQAPFDRIMVTAAAPDIPPAL
VDQLKPDGIMVLPLGDVGGIDQELVRITKTDRGIDIQPFLPVRFVPLVEGIPEE
>Mature_214_residues
MKKAEPRVIRLLMELRRMGVVDTRVLSAIERIPRALFVAEPFLDQAYENTALPIGCAQTISQPLVVGLMSQALEVGERMK
VLEIGTGSGYQAAVLAKLCRRLYSVERHKPLLAEAEARFKHLRLHNITCRAADGSRGWPEQAPFDRIMVTAAAPDIPPAL
VDQLKPDGIMVLPLGDVGGIDQELVRITKTDRGIDIQPFLPVRFVPLVEGIPEE

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=212, Percent_Identity=31.1320754716981, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1789100, Length=204, Percent_Identity=49.5098039215686, Blast_Score=182, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI71983477, Length=199, Percent_Identity=30.1507537688442, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193207222, Length=139, Percent_Identity=33.8129496402878, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17981723, Length=219, Percent_Identity=27.3972602739726, Blast_Score=74, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_MAGSA (Q2W4A2)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_421882.1
- ProteinModelPortal:   Q2W4A2
- SMR:   Q2W4A2
- STRING:   Q2W4A2
- GeneID:   3805624
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb2519
- NMPDR:   fig|342108.5.peg.2219
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   AIERIPR
- PhylomeDB:   Q2W4A2
- ProtClustDB:   CLSK943360
- BioCyc:   MMAG342108:AMB2519-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 23663; Mature: 23663

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 61-61

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAEPRVIRLLMELRRMGVVDTRVLSAIERIPRALFVAEPFLDQAYENTALPIGCAQTI
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
SQPLVVGLMSQALEVGERMKVLEIGTGSGYQAAVLAKLCRRLYSVERHKPLLAEAEARFK
HHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
HLRLHNITCRAADGSRGWPEQAPFDRIMVTAAAPDIPPALVDQLKPDGIMVLPLGDVGGI
HHHHHCEEEEECCCCCCCCCCCCHHHEEEEECCCCCCHHHHHHCCCCCEEEEECCCCCCC
DQELVRITKTDRGIDIQPFLPVRFVPLVEGIPEE
HHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCC
>Mature Secondary Structure
MKKAEPRVIRLLMELRRMGVVDTRVLSAIERIPRALFVAEPFLDQAYENTALPIGCAQTI
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
SQPLVVGLMSQALEVGERMKVLEIGTGSGYQAAVLAKLCRRLYSVERHKPLLAEAEARFK
HHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
HLRLHNITCRAADGSRGWPEQAPFDRIMVTAAAPDIPPALVDQLKPDGIMVLPLGDVGGI
HHHHHCEEEEECCCCCCCCCCCCHHHEEEEECCCCCCHHHHHHCCCCCEEEEECCCCCCC
DQELVRITKTDRGIDIQPFLPVRFVPLVEGIPEE
HHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA