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The map label for this gene is gloA [H]

Identifier: 83311582

GI number: 83311582

Start: 2661247

End: 2661639

Strand: Reverse

Name: gloA [H]

Synonym: amb2483

Alternate gene names: 83311582

Gene position: 2661639-2661247 (Counterclockwise)

Preceding gene: 83311583

Following gene: 83311579

Centisome position: 53.58

GC content: 63.1

Gene sequence:

>393_bases
ATGGCTGACTGGCGCTTTCTCCACACCATGATCCGGGTGGGTAACCTGGATCGCTCCATCCACTTCTACACCAGCCTGCT
GGGCATGAAGCTGCTGCGGCGCACGGACTACCCCGAGGGGCGCTTCACTCTGGCCTTCGTCGGCTATGGCGAGGAGGCTT
CCAACACGGTGGTCGAACTGACCCACAACTGGGACACCGAGTCCTACGAATTGGGCGGCGGCTTCGGCCATCTGGCGCTC
GGCGTGCCCGACATCTACAAGGCCTGTGCCGAGCTGGAGGCTGCCGGGGCCAAGATCACCCGTGCGCCCGGCCCCATGAA
GCATGGCTCGACCATCATCGCCTTCGTGGAAGATCCCGACGGCTATAAGATCGAGCTGATCCAGGCGAAGTGA

Upstream 100 bases:

>100_bases
CGCCGTGCCCTGGAAAAGCTGGGGCTGGGCGGCGAAAGCCCGGGCAAGCGGGCCGCCGATGCGGTGATCGACTTCATCCG
GCAAGGCAAGGAGCAACGAA

Downstream 100 bases:

>100_bases
TCGCTGCGCGAGCGGGGCAAAGCCCCGCTCGATCTCATTCCCGAATCGTATTGAGCCAGGGTGTGAGCGCTTCCCGCGCC
CGTTGGGCATAAAGCTTCTT

Product: lactoylglutathione lyase and related lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 130; Mature: 129

Protein sequence:

>130_residues
MADWRFLHTMIRVGNLDRSIHFYTSLLGMKLLRRTDYPEGRFTLAFVGYGEEASNTVVELTHNWDTESYELGGGFGHLAL
GVPDIYKACAELEAAGAKITRAPGPMKHGSTIIAFVEDPDGYKIELIQAK

Sequences:

>Translated_130_residues
MADWRFLHTMIRVGNLDRSIHFYTSLLGMKLLRRTDYPEGRFTLAFVGYGEEASNTVVELTHNWDTESYELGGGFGHLAL
GVPDIYKACAELEAAGAKITRAPGPMKHGSTIIAFVEDPDGYKIELIQAK
>Mature_129_residues
ADWRFLHTMIRVGNLDRSIHFYTSLLGMKLLRRTDYPEGRFTLAFVGYGEEASNTVVELTHNWDTESYELGGGFGHLALG
VPDIYKACAELEAAGAKITRAPGPMKHGSTIIAFVEDPDGYKIELIQAK

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=152, Percent_Identity=36.1842105263158, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1787940, Length=126, Percent_Identity=57.9365079365079, Blast_Score=161, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6323639, Length=139, Percent_Identity=41.0071942446043, Blast_Score=101, Evalue=3e-23,
Organism=Drosophila melanogaster, GI20129743, Length=154, Percent_Identity=38.3116883116883, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19920404, Length=134, Percent_Identity=33.5820895522388, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14450; Mature: 14318

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADWRFLHTMIRVGNLDRSIHFYTSLLGMKLLRRTDYPEGRFTLAFVGYGEEASNTVVEL
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEEE
THNWDTESYELGGGFGHLALGVPDIYKACAELEAAGAKITRAPGPMKHGSTIIAFVEDPD
EECCCCCCEECCCCEEEEEECCCHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCC
GYKIELIQAK
CCEEEEEECC
>Mature Secondary Structure 
ADWRFLHTMIRVGNLDRSIHFYTSLLGMKLLRRTDYPEGRFTLAFVGYGEEASNTVVEL
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEEE
THNWDTESYELGGGFGHLALGVPDIYKACAELEAAGAKITRAPGPMKHGSTIIAFVEDPD
EECCCCCCEECCCCEEEEEECCCHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCC
GYKIELIQAK
CCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]