Click here to switch to the map view.

The map label for this gene is mltB [H]

Identifier: 83311552

GI number: 83311552

Start: 2629474

End: 2630544

Strand: Reverse

Name: mltB [H]

Synonym: amb2453

Alternate gene names: 83311552

Gene position: 2630544-2629474 (Counterclockwise)

Preceding gene: 83311553

Following gene: 83311551

Centisome position: 52.96

GC content: 65.64

Gene sequence:

>1071_bases
ATGTGGCACAACACCCGGATGCTTACCAGATTTGGGGTTTGGCGCATGGCTTGGGCGACGAAATCTTGGATGATGGCCGG
GTTTGCCACGGCGGCGCTCGCCCTGGGCGGTTGCGCCGCCTCGGCCGAGGAGCCGGTGGCCCAGGCAGAGCCGCAGGTGG
CGACGGCGGAAAGCGGCTTTGGCGCCTTTCTGGCCGGCGTGCGGGCGGAAGGATTGGAGAAGGGGCTGCGGTCCCAGACC
TTGGACGCCGCCCTGGCCAACGTCACCCATATCGACCGGGTGATCGAGCTGGACCGCAAGCAGCCGGAATTCACCCTGAC
CTTCAACGAGTATCTGGGGCGCATCGTGTCCCAGGCCCGGGTCGAAGAAGGGCGGCGCAAGTTGGCCGAGAACAAGGCGC
TGCTCACCGAGATCGAGCGTCGCTATGGCGTGCAGCCCCGCTTCGTCGTGGCGCTGTGGGGCATCGAGACCAATTTCGGC
AAGAACACCGGCGGCATGTCGGTGGTGTCGTCCCTGGCGACCCTGGCCTATGACGGACGGCGCTCGAAATATTTCCGCAC
CGAGTTGATGAATGCGCTGACCATCCTCGACCAGGGCCATATCCAGCCGGCCAACATGATCGGCTCGTGGGCCGGGGCCA
TGGGCCAGTGCCAGTTCATGCCGTCCACCTTCCTTAAGTTCGCGGTGGACTGGGATGGCGACGGCAAGCGCAACATCTGG
ACCAACCGTGCCGATGCCCTGGCCTCGGCGGCCAATTACCTGTCGTCGGAGGGCTGGAAGGGTGACCAGACCTGGGGACG
GGCGGTAAAGCTGCCCGCCGACTTCCCGCGTACCGCCCTGGGCGTCGATACCCGCAAGACGGTGAAGGAATGGGCGGCCC
TGGGGGTCAAGGGCTCGGACGGCAAGCCGTTGCCGGCCCGCGACATCAGCGCCTCCATCGTCCTGGCCGAGGGCACCAAG
GGTCCGGCCTTCCTGGTCTATGACAATTTCCGCACCATCATGAAGTGGAACCGCTCCACCTTCTTCGCCTTGGCGGCGGG
GCATCTTGCGGACCGCATCGGTGGCAAGTAG

Upstream 100 bases:

>100_bases
GACCGCTGATCCACGAAGGCCTGGGGCTGATCATCTACCGCCTGATGGGGTGGACCGATACGCTGCTGCCACAGCCTTAA
CATTGCGCTGGGCCGCTTAA

Downstream 100 bases:

>100_bases
ACTACAACATCTTTGGGCAAGCAGGCGGGGAGGGGACTAGATGTCGCGGAGGACGCGCTTGGCGGCTTTGCTGCTGTCTG
GGACCGTGCTGACGGGCTGC

Product: membrane-bound lytic murein transglycosylase B

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MWHNTRMLTRFGVWRMAWATKSWMMAGFATAALALGGCAASAEEPVAQAEPQVATAESGFGAFLAGVRAEGLEKGLRSQT
LDAALANVTHIDRVIELDRKQPEFTLTFNEYLGRIVSQARVEEGRRKLAENKALLTEIERRYGVQPRFVVALWGIETNFG
KNTGGMSVVSSLATLAYDGRRSKYFRTELMNALTILDQGHIQPANMIGSWAGAMGQCQFMPSTFLKFAVDWDGDGKRNIW
TNRADALASAANYLSSEGWKGDQTWGRAVKLPADFPRTALGVDTRKTVKEWAALGVKGSDGKPLPARDISASIVLAEGTK
GPAFLVYDNFRTIMKWNRSTFFALAAGHLADRIGGK

Sequences:

>Translated_356_residues
MWHNTRMLTRFGVWRMAWATKSWMMAGFATAALALGGCAASAEEPVAQAEPQVATAESGFGAFLAGVRAEGLEKGLRSQT
LDAALANVTHIDRVIELDRKQPEFTLTFNEYLGRIVSQARVEEGRRKLAENKALLTEIERRYGVQPRFVVALWGIETNFG
KNTGGMSVVSSLATLAYDGRRSKYFRTELMNALTILDQGHIQPANMIGSWAGAMGQCQFMPSTFLKFAVDWDGDGKRNIW
TNRADALASAANYLSSEGWKGDQTWGRAVKLPADFPRTALGVDTRKTVKEWAALGVKGSDGKPLPARDISASIVLAEGTK
GPAFLVYDNFRTIMKWNRSTFFALAAGHLADRIGGK
>Mature_356_residues
MWHNTRMLTRFGVWRMAWATKSWMMAGFATAALALGGCAASAEEPVAQAEPQVATAESGFGAFLAGVRAEGLEKGLRSQT
LDAALANVTHIDRVIELDRKQPEFTLTFNEYLGRIVSQARVEEGRRKLAENKALLTEIERRYGVQPRFVVALWGIETNFG
KNTGGMSVVSSLATLAYDGRRSKYFRTELMNALTILDQGHIQPANMIGSWAGAMGQCQFMPSTFLKFAVDWDGDGKRNIW
TNRADALASAANYLSSEGWKGDQTWGRAVKLPADFPRTALGVDTRKTVKEWAALGVKGSDGKPLPARDISASIVLAEGTK
GPAFLVYDNFRTIMKWNRSTFFALAAGHLADRIGGK

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=321, Percent_Identity=30.5295950155763, Blast_Score=132, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 38991; Mature: 38991

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWHNTRMLTRFGVWRMAWATKSWMMAGFATAALALGGCAASAEEPVAQAEPQVATAESGF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEECCCCH
GAFLAGVRAEGLEKGLRSQTLDAALANVTHIDRVIELDRKQPEFTLTFNEYLGRIVSQAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHH
VEEGRRKLAENKALLTEIERRYGVQPRFVVALWGIETNFGKNTGGMSVVSSLATLAYDGR
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCC
RSKYFRTELMNALTILDQGHIQPANMIGSWAGAMGQCQFMPSTFLKFAVDWDGDGKRNIW
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEECHHHHEEEEEECCCCCCCCCC
TNRADALASAANYLSSEGWKGDQTWGRAVKLPADFPRTALGVDTRKTVKEWAALGVKGSD
CCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCHHHCCCHHHHHHHHHHCCCCCCC
GKPLPARDISASIVLAEGTKGPAFLVYDNFRTIMKWNRSTFFALAAGHLADRIGGK
CCCCCCCCCCEEEEEEECCCCCEEEEECCCHHHHHCCCCCEEEHHHHHHHHHHCCC
>Mature Secondary Structure
MWHNTRMLTRFGVWRMAWATKSWMMAGFATAALALGGCAASAEEPVAQAEPQVATAESGF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEECCCCH
GAFLAGVRAEGLEKGLRSQTLDAALANVTHIDRVIELDRKQPEFTLTFNEYLGRIVSQAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHH
VEEGRRKLAENKALLTEIERRYGVQPRFVVALWGIETNFGKNTGGMSVVSSLATLAYDGR
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCC
RSKYFRTELMNALTILDQGHIQPANMIGSWAGAMGQCQFMPSTFLKFAVDWDGDGKRNIW
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEECHHHHEEEEEECCCCCCCCCC
TNRADALASAANYLSSEGWKGDQTWGRAVKLPADFPRTALGVDTRKTVKEWAALGVKGSD
CCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCHHHCCCHHHHHHHHHHCCCCCCC
GKPLPARDISASIVLAEGTKGPAFLVYDNFRTIMKWNRSTFFALAAGHLADRIGGK
CCCCCCCCCCEEEEEEECCCCCEEEEECCCHHHHHCCCCCEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]