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The map label for this gene is metG [H]

Identifier: 83311547

GI number: 83311547

Start: 2623962

End: 2625512

Strand: Reverse

Name: metG [H]

Synonym: amb2448

Alternate gene names: 83311547

Gene position: 2625512-2623962 (Counterclockwise)

Preceding gene: 83311548

Following gene: 83311546

Centisome position: 52.86

GC content: 63.96

Gene sequence:

>1551_bases
ATGACCGGGGCCGGGACCTTCTACGTCACCACGCCGATCTACTACGTCAATGACAAGCCCCATATCGGCCATGCCTATAC
TACGCTGGCCTGCGATGTGCTGGCGCGCTTCAAGCGCCTGGACGGCTTCGACGTCAAGTTCCTGACCGGCACCGACGAGC
ATGGTCAGAAGGTGGAGAAGTCGGCGGAAGCCTTCGGCGTCACCCCTCAGGCCCTGGCCGACCAGAATTCCGCCAATTTC
CGTGAGCTGGCCAAGATCCTGGGCTGCACCAACGACGATTTCATCCGCACCACCGAGGCTCGCCACAAGGCCGCCTGTCA
GGCGCTGTGGGACAAGCTGGTGGCCAAGGGCGACATTTATCTCGGCGCCTATGAGGGCTGGTACTCGGTGCGTGATGAGG
CCTTCTACGCCGAGGATGAACTGGTCAAGGGCGCCGACGGCGCCAAGCTGGCCCCCACCGGCGCACCGGTGGAATGGGTG
AAGGAACCCAGCTACTTCTTCCGCCTGTCCGCCTGGCAGGACCGCCTGCTCCGGTGGTACGAGGACAATCCCGATTGCGT
GGCGCCCCAATCGCGCCGCAACGAGGTGATGAGCTTCGTGAAGGGCGGGCTGCAGGACCTGTCGGTGTCGCGCACCAGCT
TCAAATGGGGCATTCCGGTGCCGGGCGACGATGCCCACATCATGTATGTCTGGCTGGACGCCCTGACCAATTACATCACC
GCCGTGGGCTATCCCGACACCACCGGCGATTTCGCCCGCTACTGGCCCAACGTCATTCATATGGTGGGCAAGGACATCGT
TCGCTTCCACGCCGTCTACTGGCCGGCTTTCCTGATGGCCGCCGACCTGCCGGCGCCCAAGCGGGTCTTCGCCCATGGCT
GGTGGACCAACGAGGGCCAGAAGATCTCTAAATCGGTGGGCAACGTCATCGACCCGCTGTCGCTGATCGAGACCTACGGC
CTGGATCAGGTGCGCTATTTCCTGCTGCGCGAAGTGCCGTTCGGCAATGACGGCGATTTCAGCCATCGCGCCATGATGGG
CCGCATGAACAGCGAGTTGGCCAATGACTATGGCAATCTGGTCCAGCGCTCGCTGTCCATGATCGGCAAGAATTGCGGCG
GCGTGACGCCCGCCCATTTGCCCGATACCGACGAGGACAAGGAAATGCTGGGCGCCGCCTATGGCATGCTGGCCAAGGTG
CGGGACGCCATGGACCGCCAGCTCTACCACGAAGCCATCGAGGCCATTTGGGTGGTGGTCCGCGCCGCCAATGCCTATGT
GGATAAGCAGGCGCCCTGGGCGCTGAAGAAGACCGATCCCGCCCGCATGGGCACGGTGCTCTGGGTTCTGGCCGAGACCA
TCCGCTGCCTGGCCCTGCTGACCCAGCCGTTCATGCCGGCGGCCTCGGCCCGCATCCTCGACCAGTTGATGGTGCCCGAG
GCCGAGCGGAGCTTCGCCTTCTTCGGCCCCGGTCATGCCCTGAAGGCCGGGATTTCCCTGCCGACGCCCCAGGGCGTGTT
CCCGCGCTATGTGGAAGAGGTTTCCGCCTAA

Upstream 100 bases:

>100_bases
ACCGCAAGCAGGCCGTTCTCGGCGCCTTCATGGCGGTTGAACGCCTCGCCCGCCAGGGTTCAAGATAGCGGGCAACAACT
TGATTTAGCGGAGCATTCCC

Downstream 100 bases:

>100_bases
ATGCTGGTCGACAGCCACTGCCACCTGGATTTTCCCGATTTCGCCGACGACCTCGATGGCGTGGTCGGGCGCGCGGGCGC
GGCGGGAGTAGGGGTGCTGC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MTGAGTFYVTTPIYYVNDKPHIGHAYTTLACDVLARFKRLDGFDVKFLTGTDEHGQKVEKSAEAFGVTPQALADQNSANF
RELAKILGCTNDDFIRTTEARHKAACQALWDKLVAKGDIYLGAYEGWYSVRDEAFYAEDELVKGADGAKLAPTGAPVEWV
KEPSYFFRLSAWQDRLLRWYEDNPDCVAPQSRRNEVMSFVKGGLQDLSVSRTSFKWGIPVPGDDAHIMYVWLDALTNYIT
AVGYPDTTGDFARYWPNVIHMVGKDIVRFHAVYWPAFLMAADLPAPKRVFAHGWWTNEGQKISKSVGNVIDPLSLIETYG
LDQVRYFLLREVPFGNDGDFSHRAMMGRMNSELANDYGNLVQRSLSMIGKNCGGVTPAHLPDTDEDKEMLGAAYGMLAKV
RDAMDRQLYHEAIEAIWVVVRAANAYVDKQAPWALKKTDPARMGTVLWVLAETIRCLALLTQPFMPAASARILDQLMVPE
AERSFAFFGPGHALKAGISLPTPQGVFPRYVEEVSA

Sequences:

>Translated_516_residues
MTGAGTFYVTTPIYYVNDKPHIGHAYTTLACDVLARFKRLDGFDVKFLTGTDEHGQKVEKSAEAFGVTPQALADQNSANF
RELAKILGCTNDDFIRTTEARHKAACQALWDKLVAKGDIYLGAYEGWYSVRDEAFYAEDELVKGADGAKLAPTGAPVEWV
KEPSYFFRLSAWQDRLLRWYEDNPDCVAPQSRRNEVMSFVKGGLQDLSVSRTSFKWGIPVPGDDAHIMYVWLDALTNYIT
AVGYPDTTGDFARYWPNVIHMVGKDIVRFHAVYWPAFLMAADLPAPKRVFAHGWWTNEGQKISKSVGNVIDPLSLIETYG
LDQVRYFLLREVPFGNDGDFSHRAMMGRMNSELANDYGNLVQRSLSMIGKNCGGVTPAHLPDTDEDKEMLGAAYGMLAKV
RDAMDRQLYHEAIEAIWVVVRAANAYVDKQAPWALKKTDPARMGTVLWVLAETIRCLALLTQPFMPAASARILDQLMVPE
AERSFAFFGPGHALKAGISLPTPQGVFPRYVEEVSA
>Mature_515_residues
TGAGTFYVTTPIYYVNDKPHIGHAYTTLACDVLARFKRLDGFDVKFLTGTDEHGQKVEKSAEAFGVTPQALADQNSANFR
ELAKILGCTNDDFIRTTEARHKAACQALWDKLVAKGDIYLGAYEGWYSVRDEAFYAEDELVKGADGAKLAPTGAPVEWVK
EPSYFFRLSAWQDRLLRWYEDNPDCVAPQSRRNEVMSFVKGGLQDLSVSRTSFKWGIPVPGDDAHIMYVWLDALTNYITA
VGYPDTTGDFARYWPNVIHMVGKDIVRFHAVYWPAFLMAADLPAPKRVFAHGWWTNEGQKISKSVGNVIDPLSLIETYGL
DQVRYFLLREVPFGNDGDFSHRAMMGRMNSELANDYGNLVQRSLSMIGKNCGGVTPAHLPDTDEDKEMLGAAYGMLAKVR
DAMDRQLYHEAIEAIWVVVRAANAYVDKQAPWALKKTDPARMGTVLWVLAETIRCLALLTQPFMPAASARILDQLMVPEA
ERSFAFFGPGHALKAGISLPTPQGVFPRYVEEVSA

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=506, Percent_Identity=40.3162055335968, Blast_Score=381, Evalue=1e-106,
Organism=Homo sapiens, GI14043022, Length=550, Percent_Identity=26.3636363636364, Blast_Score=166, Evalue=7e-41,
Organism=Escherichia coli, GI1788432, Length=506, Percent_Identity=25.098814229249, Blast_Score=174, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI212645096, Length=516, Percent_Identity=36.6279069767442, Blast_Score=330, Evalue=8e-91,
Organism=Caenorhabditis elegans, GI71992781, Length=410, Percent_Identity=34.6341463414634, Blast_Score=240, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17540794, Length=555, Percent_Identity=29.1891891891892, Blast_Score=167, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6321610, Length=533, Percent_Identity=33.9587242026266, Blast_Score=314, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6321704, Length=517, Percent_Identity=24.3713733075435, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21358483, Length=544, Percent_Identity=37.6838235294118, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655311, Length=547, Percent_Identity=25.9597806215722, Blast_Score=150, Evalue=3e-36,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 57598; Mature: 57467

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGAGTFYVTTPIYYVNDKPHIGHAYTTLACDVLARFKRLDGFDVKFLTGTDEHGQKVEK
CCCCCEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHH
SAEAFGVTPQALADQNSANFRELAKILGCTNDDFIRTTEARHKAACQALWDKLVAKGDIY
HHHHHCCCHHHHCCCCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEE
LGAYEGWYSVRDEAFYAEDELVKGADGAKLAPTGAPVEWVKEPSYFFRLSAWQDRLLRWY
EEEECCCHHHCCCHHCCHHHHHCCCCCCEECCCCCCHHHHCCCCCEEEEHHHHHHHHHHH
EDNPDCVAPQSRRNEVMSFVKGGLQDLSVSRTSFKWGIPVPGDDAHIMYVWLDALTNYIT
CCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCCEEECCCCCCCCCEEEEHHHHHHHHHHH
AVGYPDTTGDFARYWPNVIHMVGKDIVRFHAVYWPAFLMAADLPAPKRVFAHGWWTNEGQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCH
KISKSVGNVIDPLSLIETYGLDQVRYFLLREVPFGNDGDFSHRAMMGRMNSELANDYGNL
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VQRSLSMIGKNCGGVTPAHLPDTDEDKEMLGAAYGMLAKVRDAMDRQLYHEAIEAIWVVV
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAANAYVDKQAPWALKKTDPARMGTVLWVLAETIRCLALLTQPFMPAASARILDQLMVPE
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
AERSFAFFGPGHALKAGISLPTPQGVFPRYVEEVSA
CCCCEEEECCCHHHHCCCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
TGAGTFYVTTPIYYVNDKPHIGHAYTTLACDVLARFKRLDGFDVKFLTGTDEHGQKVEK
CCCCEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHH
SAEAFGVTPQALADQNSANFRELAKILGCTNDDFIRTTEARHKAACQALWDKLVAKGDIY
HHHHHCCCHHHHCCCCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEE
LGAYEGWYSVRDEAFYAEDELVKGADGAKLAPTGAPVEWVKEPSYFFRLSAWQDRLLRWY
EEEECCCHHHCCCHHCCHHHHHCCCCCCEECCCCCCHHHHCCCCCEEEEHHHHHHHHHHH
EDNPDCVAPQSRRNEVMSFVKGGLQDLSVSRTSFKWGIPVPGDDAHIMYVWLDALTNYIT
CCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCCEEECCCCCCCCCEEEEHHHHHHHHHHH
AVGYPDTTGDFARYWPNVIHMVGKDIVRFHAVYWPAFLMAADLPAPKRVFAHGWWTNEGQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCH
KISKSVGNVIDPLSLIETYGLDQVRYFLLREVPFGNDGDFSHRAMMGRMNSELANDYGNL
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VQRSLSMIGKNCGGVTPAHLPDTDEDKEMLGAAYGMLAKVRDAMDRQLYHEAIEAIWVVV
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAANAYVDKQAPWALKKTDPARMGTVLWVLAETIRCLALLTQPFMPAASARILDQLMVPE
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
AERSFAFFGPGHALKAGISLPTPQGVFPRYVEEVSA
CCCCEEEECCCHHHHCCCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]