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The map label for this gene is holB [H]

Identifier: 83311548

GI number: 83311548

Start: 2625545

End: 2626636

Strand: Reverse

Name: holB [H]

Synonym: amb2449

Alternate gene names: 83311548

Gene position: 2626636-2625545 (Counterclockwise)

Preceding gene: 83311549

Following gene: 83311547

Centisome position: 52.88

GC content: 68.5

Gene sequence:

>1092_bases
ATGACCGACTCTCCACACCCTCGCGAGACCTCCGAGCTGTTCGGCCACGGAGCGGCCGAGTCGGCGCTGCTGGATGCCTG
GAACTCGGGCCGGCTGGCCCATGCCTGGCTGCTGTGCGGCCCCAAGGGCATCGGCAAGGCGACCCTGGCCTACCGCTTCG
CCCGCTTCGCCCTGGCCGGCGGCGGGGACGGTGGGGGGCTGTTCGGCGATGTGCCGTCCAGCCTGGAGATCCGCCCCGAT
CACCCAGTGTTCCGCCGCATCGCCTCCCAGGGCCATGCCGACCTGGAGACGGTGGAGCGTGGATGGGCCGATGACAAGAA
GAGCAAGCTGCGCTCGGAGATCGTGGTCGAGGACGTGCGCGGCATCGGCCACTTCATGTCCCTGACCCCGGCCGAGGGCG
GCTGGCGGGTGGTGATCATCGATTCCGCCGACGAAATGAACCGCAATGCCGCCAATGCGGTGCTGAAAGTTCTGGAAGAG
CCGCCGCGCAACGCCTTGATGTTGCTGATTTCCCATTCGCCGGGCCGGTTGCTGCCCACCATCCGGTCGCGCTGCCGCCG
CCTGATGCTTCAGCCCCTGGGCGAAGATGTGGTCGCCGACCTTTTGACCCGCCAGCGCCCGGAAGTGGGACCCACGGAAG
TGGCGGCCCTGGCCAAGCTGGGCGAGGGCAGCATCGGCAAGGCCCTGGCCCTGGCCGACGAAGGCGGGCTGGACCTGTAC
CGGGACTTGCTGAACCTGCTGCAAGGGCTGCCGCGCCTGGATGTGCCGGCGCTGCATGCCTTCGGCGACAAGGTGGCAAG
GCCCGAAAACGATGGATCGTTCCGTACCGTCACCGAATTGCTGGGCTGGTGGCTGGCCCGCCTGATTCGCGCCGGCGGGC
GCCAGGGGGCCGGAATGGCCGAGGTGGTGGCGGGCGAGGGCGCCCTGATGGAGCGCCTGCTGGCGGCGGCCAGCCTTGAA
CATTGGCTGGAGGTGTGGGAAAAGATCACCACCCTGTTCGCCCGCACCGAAGCGGTTCATCTCGACCGCAAGCAGGCCGT
TCTCGGCGCCTTCATGGCGGTTGAACGCCTCGCCCGCCAGGGTTCAAGATAG

Upstream 100 bases:

>100_bases
CCGCCAATCCCATGCGCTGCGCCGTCATCGACGCCACCCGGTCGCCTGACGAAGTGCACGGCGACATCATGTCCACGGTC
AGGGCGCGGCTGCCGGTGCC

Downstream 100 bases:

>100_bases
CGGGCAACAACTTGATTTAGCGGAGCATTCCCATGACCGGGGCCGGGACCTTCTACGTCACCACGCCGATCTACTACGTC
AATGACAAGCCCCATATCGG

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MTDSPHPRETSELFGHGAAESALLDAWNSGRLAHAWLLCGPKGIGKATLAYRFARFALAGGGDGGGLFGDVPSSLEIRPD
HPVFRRIASQGHADLETVERGWADDKKSKLRSEIVVEDVRGIGHFMSLTPAEGGWRVVIIDSADEMNRNAANAVLKVLEE
PPRNALMLLISHSPGRLLPTIRSRCRRLMLQPLGEDVVADLLTRQRPEVGPTEVAALAKLGEGSIGKALALADEGGLDLY
RDLLNLLQGLPRLDVPALHAFGDKVARPENDGSFRTVTELLGWWLARLIRAGGRQGAGMAEVVAGEGALMERLLAAASLE
HWLEVWEKITTLFARTEAVHLDRKQAVLGAFMAVERLARQGSR

Sequences:

>Translated_363_residues
MTDSPHPRETSELFGHGAAESALLDAWNSGRLAHAWLLCGPKGIGKATLAYRFARFALAGGGDGGGLFGDVPSSLEIRPD
HPVFRRIASQGHADLETVERGWADDKKSKLRSEIVVEDVRGIGHFMSLTPAEGGWRVVIIDSADEMNRNAANAVLKVLEE
PPRNALMLLISHSPGRLLPTIRSRCRRLMLQPLGEDVVADLLTRQRPEVGPTEVAALAKLGEGSIGKALALADEGGLDLY
RDLLNLLQGLPRLDVPALHAFGDKVARPENDGSFRTVTELLGWWLARLIRAGGRQGAGMAEVVAGEGALMERLLAAASLE
HWLEVWEKITTLFARTEAVHLDRKQAVLGAFMAVERLARQGSR
>Mature_362_residues
TDSPHPRETSELFGHGAAESALLDAWNSGRLAHAWLLCGPKGIGKATLAYRFARFALAGGGDGGGLFGDVPSSLEIRPDH
PVFRRIASQGHADLETVERGWADDKKSKLRSEIVVEDVRGIGHFMSLTPAEGGWRVVIIDSADEMNRNAANAVLKVLEEP
PRNALMLLISHSPGRLLPTIRSRCRRLMLQPLGEDVVADLLTRQRPEVGPTEVAALAKLGEGSIGKALALADEGGLDLYR
DLLNLLQGLPRLDVPALHAFGDKVARPENDGSFRTVTELLGWWLARLIRAGGRQGAGMAEVVAGEGALMERLLAAASLEH
WLEVWEKITTLFARTEAVHLDRKQAVLGAFMAVERLARQGSR

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=233, Percent_Identity=27.8969957081545, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 39347; Mature: 39215

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSPHPRETSELFGHGAAESALLDAWNSGRLAHAWLLCGPKGIGKATLAYRFARFALAG
CCCCCCCCCHHHHHCCCCHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCC
GGDGGGLFGDVPSSLEIRPDHPVFRRIASQGHADLETVERGWADDKKSKLRSEIVVEDVR
CCCCCCCCCCCCCCCEECCCCHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHH
GIGHFMSLTPAEGGWRVVIIDSADEMNRNAANAVLKVLEEPPRNALMLLISHSPGRLLPT
HHHHHHCCCCCCCCEEEEEECCCHHHCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHH
IRSRCRRLMLQPLGEDVVADLLTRQRPEVGPTEVAALAKLGEGSIGKALALADEGGLDLY
HHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCHHHH
RDLLNLLQGLPRLDVPALHAFGDKVARPENDGSFRTVTELLGWWLARLIRAGGRQGAGMA
HHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHH
EVVAGEGALMERLLAAASLEHWLEVWEKITTLFARTEAVHLDRKQAVLGAFMAVERLARQ
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSR
CCC
>Mature Secondary Structure 
TDSPHPRETSELFGHGAAESALLDAWNSGRLAHAWLLCGPKGIGKATLAYRFARFALAG
CCCCCCCCHHHHHCCCCHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCC
GGDGGGLFGDVPSSLEIRPDHPVFRRIASQGHADLETVERGWADDKKSKLRSEIVVEDVR
CCCCCCCCCCCCCCCEECCCCHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHH
GIGHFMSLTPAEGGWRVVIIDSADEMNRNAANAVLKVLEEPPRNALMLLISHSPGRLLPT
HHHHHHCCCCCCCCEEEEEECCCHHHCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHH
IRSRCRRLMLQPLGEDVVADLLTRQRPEVGPTEVAALAKLGEGSIGKALALADEGGLDLY
HHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCHHHH
RDLLNLLQGLPRLDVPALHAFGDKVARPENDGSFRTVTELLGWWLARLIRAGGRQGAGMA
HHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHH
EVVAGEGALMERLLAAASLEHWLEVWEKITTLFARTEAVHLDRKQAVLGAFMAVERLARQ
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GSR
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]