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The map label for this gene is livM [H]

Identifier: 83311424

GI number: 83311424

Start: 2527924

End: 2528874

Strand: Reverse

Name: livM [H]

Synonym: amb2325

Alternate gene names: 83311424

Gene position: 2528874-2527924 (Counterclockwise)

Preceding gene: 83311425

Following gene: 83311423

Centisome position: 50.91

GC content: 63.93

Gene sequence:

>951_bases
ATGACCCTGCCCTCCTCCGTGTCCTCGCGTCAGAAGCCCTTCGTCCTCGCCCTGCTCGGCCTGGGGCTGGTGCTGCCGCT
GGCCGTCTATCCCGTCTTCCTGATGAAGGGATTGTGCTTCGGCCTGTTCGCCGCCGCCTTCAACCTGCTGTTGGGCTATG
TGGGGCTGCTGTCTTTCGGACACGCCATGTTCTTCGGCTGGTCGGCCTATGTCACCGCCTATGCCGCCAAGGAATGGGGC
CTCACTCCCGAACTGGCCATCCTGCTGGGGGTCGCCCTGGCCACGGCGCTGGGCGCCGTGGTGGGCTGGCTGGCCATCCG
CCGCCAGGGTATCTATTTCGCCATGATCACCCTGGCCCTGGCCCAACTGGGCTTCTTCGTGGCGGTGCAGGCTCCCATGA
CCCATGGCGAGGACGGTATCCAGGGCGTACCCCGCGGCATGCTATTCGGACTGATCGACCTGTCCAACACCATGGCCATG
TACTACTTCGTGCTGGCGGTCTTCGTCCTGTCCCTGCTGTTCCTCCACCGGGTCGTCAACTCCCCCTTCGGTCAGGTGCT
GAAGGCCATCCGCGACAACGAGCCCCGCGCCATCTCCCTGGGCTACGAGGTGACCCGCTACAAGCTGATCGCCTTCGTGC
TGTCCTCGGCCTTCGCCGGACTGGCGGGCTCGCTGAAGAGTCTGGTGTTCCAGTTGGCTTCGCTGGCCGATGTCAGCTGG
CACATGTCGGGCGAGGTGGTGCTGATGACCTTGCTGGGGGGCGTGGGCACCGTCCTCGGCCCCATGGTTGGCGCTTTCCT
GGTGGTGGCCATCCAGGACTTCTTCGCCGGCATCGGGTCATGGGTCATCATTATCCAAGGTATTATCTTCGTCGTTTGCG
TCCTGTTGTTCCGTAGCGGTATGATCGGCGTGCTGGCGCCCTGGCTGAAAAGGCGCGGCATTACTGGCTGA

Upstream 100 bases:

>100_bases
GTGTTCTATCCCGAAGCCGCAAGTACCGTGATCTTCATGGTCATGGTGGTGGTCCTTCTGATCAAACCCGCCGGGTTGTT
CGGCAAGGGAGCCTGACGCC

Downstream 100 bases:

>100_bases
GGTGTGGAGAAGAGTTCGAGCATGCCGGAAACCGTCTTGCGTCGCCGTGAAATGCTGGCGAACACGCCCCTGTTTGCGTC
CGTCCAGACCGACTTGCTCG

Product: ABC-type branched-chain amino acid transport system, permease component

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MTLPSSVSSRQKPFVLALLGLGLVLPLAVYPVFLMKGLCFGLFAAAFNLLLGYVGLLSFGHAMFFGWSAYVTAYAAKEWG
LTPELAILLGVALATALGAVVGWLAIRRQGIYFAMITLALAQLGFFVAVQAPMTHGEDGIQGVPRGMLFGLIDLSNTMAM
YYFVLAVFVLSLLFLHRVVNSPFGQVLKAIRDNEPRAISLGYEVTRYKLIAFVLSSAFAGLAGSLKSLVFQLASLADVSW
HMSGEVVLMTLLGGVGTVLGPMVGAFLVVAIQDFFAGIGSWVIIIQGIIFVVCVLLFRSGMIGVLAPWLKRRGITG

Sequences:

>Translated_316_residues
MTLPSSVSSRQKPFVLALLGLGLVLPLAVYPVFLMKGLCFGLFAAAFNLLLGYVGLLSFGHAMFFGWSAYVTAYAAKEWG
LTPELAILLGVALATALGAVVGWLAIRRQGIYFAMITLALAQLGFFVAVQAPMTHGEDGIQGVPRGMLFGLIDLSNTMAM
YYFVLAVFVLSLLFLHRVVNSPFGQVLKAIRDNEPRAISLGYEVTRYKLIAFVLSSAFAGLAGSLKSLVFQLASLADVSW
HMSGEVVLMTLLGGVGTVLGPMVGAFLVVAIQDFFAGIGSWVIIIQGIIFVVCVLLFRSGMIGVLAPWLKRRGITG
>Mature_315_residues
TLPSSVSSRQKPFVLALLGLGLVLPLAVYPVFLMKGLCFGLFAAAFNLLLGYVGLLSFGHAMFFGWSAYVTAYAAKEWGL
TPELAILLGVALATALGAVVGWLAIRRQGIYFAMITLALAQLGFFVAVQAPMTHGEDGIQGVPRGMLFGLIDLSNTMAMY
YFVLAVFVLSLLFLHRVVNSPFGQVLKAIRDNEPRAISLGYEVTRYKLIAFVLSSAFAGLAGSLKSLVFQLASLADVSWH
MSGEVVLMTLLGGVGTVLGPMVGAFLVVAIQDFFAGIGSWVIIIQGIIFVVCVLLFRSGMIGVLAPWLKRRGITG

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=289, Percent_Identity=26.643598615917, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 33808; Mature: 33677

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPSSVSSRQKPFVLALLGLGLVLPLAVYPVFLMKGLCFGLFAAAFNLLLGYVGLLSFG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAMFFGWSAYVTAYAAKEWGLTPELAILLGVALATALGAVVGWLAIRRQGIYFAMITLAL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
AQLGFFVAVQAPMTHGEDGIQGVPRGMLFGLIDLSNTMAMYYFVLAVFVLSLLFLHRVVN
HHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SPFGQVLKAIRDNEPRAISLGYEVTRYKLIAFVLSSAFAGLAGSLKSLVFQLASLADVSW
CCHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HMSGEVVLMTLLGGVGTVLGPMVGAFLVVAIQDFFAGIGSWVIIIQGIIFVVCVLLFRSG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MIGVLAPWLKRRGITG
HHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TLPSSVSSRQKPFVLALLGLGLVLPLAVYPVFLMKGLCFGLFAAAFNLLLGYVGLLSFG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAMFFGWSAYVTAYAAKEWGLTPELAILLGVALATALGAVVGWLAIRRQGIYFAMITLAL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
AQLGFFVAVQAPMTHGEDGIQGVPRGMLFGLIDLSNTMAMYYFVLAVFVLSLLFLHRVVN
HHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SPFGQVLKAIRDNEPRAISLGYEVTRYKLIAFVLSSAFAGLAGSLKSLVFQLASLADVSW
CCHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HMSGEVVLMTLLGGVGTVLGPMVGAFLVVAIQDFFAGIGSWVIIIQGIIFVVCVLLFRSG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MIGVLAPWLKRRGITG
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]