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The map label for this gene is ntcA [H]

Identifier: 83311423

GI number: 83311423

Start: 2527201

End: 2527920

Strand: Reverse

Name: ntcA [H]

Synonym: amb2324

Alternate gene names: 83311423

Gene position: 2527920-2527201 (Counterclockwise)

Preceding gene: 83311424

Following gene: 83311414

Centisome position: 50.89

GC content: 63.19

Gene sequence:

>720_bases
GTGGAGAAGAGTTCGAGCATGCCGGAAACCGTCTTGCGTCGCCGTGAAATGCTGGCGAACACGCCCCTGTTTGCGTCCGT
CCAGACCGACTTGCTCGACGAATTGGCCGCCAAGGCCAAGATGGTCAAGGTCGATGCGCGCGAAACGCTGTTTTCCAAGG
GCGATCCGGGAGACCGACTCTATCTGGTGGCCAAGGGCCTGATCCGCATCGGAGTCCTGTCCGCCGACGGGCGCGAGGTC
ACCTATGGCCTGATCAAGCCGGGCCAGTTGTTCGGCGAGATCGCCGTTCTGGACGGCAAGGAGCGCTCGGCCGACGCCAC
CGCCATGGAAGCTACCGAGTTAATCGCGTTGGAACGCAAGGACGTCCACACCTTCCTGCATCGCCATCCGGCCCAGGCGC
TGCACCTGATCGAGGTGCTGTGCGAACGCATCCGGCGTGCCGATAACCAGCTGGAAGACATGGTGTTCCTGTCCCTGCCC
TCCCGCCTGGCCAAGCATCTGTTGATGCTGGTCCAGACCATGGGGACCAAGGCCAAGCCCGGGACGCCGTCCGCCATCAA
GCTGTCGCAGCAGGAAATCGCCGACCACCTGGGCATCAGCCGCGAAAGCGTCAACAAGGTTCTGTCCAAGTGGGAACAGG
CCGGCATCGTCACCTTGGGCCGCGGCCAGATCACCCTGAACAAGACCGCCGCGCTGGAGGGGCTGACTTCCCTGGAATGA

Upstream 100 bases:

>100_bases
CCAAGGTATTATCTTCGTCGTTTGCGTCCTGTTGTTCCGTAGCGGTATGATCGGCGTGCTGGCGCCCTGGCTGAAAAGGC
GCGGCATTACTGGCTGAGGT

Downstream 100 bases:

>100_bases
GTCTCCGCCTTATGTGAGACTCTTCACAGACGCCATCGCCCCCCGCCCATACATTGGGCCTGTCACGAATTCCAGTCCGT
CCCCTCCTCGAACGAACGAA

Product: cAMP-binding protein - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MEKSSSMPETVLRRREMLANTPLFASVQTDLLDELAAKAKMVKVDARETLFSKGDPGDRLYLVAKGLIRIGVLSADGREV
TYGLIKPGQLFGEIAVLDGKERSADATAMEATELIALERKDVHTFLHRHPAQALHLIEVLCERIRRADNQLEDMVFLSLP
SRLAKHLLMLVQTMGTKAKPGTPSAIKLSQQEIADHLGISRESVNKVLSKWEQAGIVTLGRGQITLNKTAALEGLTSLE

Sequences:

>Translated_239_residues
MEKSSSMPETVLRRREMLANTPLFASVQTDLLDELAAKAKMVKVDARETLFSKGDPGDRLYLVAKGLIRIGVLSADGREV
TYGLIKPGQLFGEIAVLDGKERSADATAMEATELIALERKDVHTFLHRHPAQALHLIEVLCERIRRADNQLEDMVFLSLP
SRLAKHLLMLVQTMGTKAKPGTPSAIKLSQQEIADHLGISRESVNKVLSKWEQAGIVTLGRGQITLNKTAALEGLTSLE
>Mature_239_residues
MEKSSSMPETVLRRREMLANTPLFASVQTDLLDELAAKAKMVKVDARETLFSKGDPGDRLYLVAKGLIRIGVLSADGREV
TYGLIKPGQLFGEIAVLDGKERSADATAMEATELIALERKDVHTFLHRHPAQALHLIEVLCERIRRADNQLEDMVFLSLP
SRLAKHLLMLVQTMGTKAKPGTPSAIKLSQQEIADHLGISRESVNKVLSKWEQAGIVTLGRGQITLNKTAALEGLTSLE

Specific function: Required for full expression of proteins subject to ammonium repression. Transcriptional activator of genes subject to nitrogen control [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=193, Percent_Identity=25.3886010362694, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR022299
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 26260; Mature: 26260

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00889 CNMP_BINDING_2 ; PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKSSSMPETVLRRREMLANTPLFASVQTDLLDELAAKAKMVKVDARETLFSKGDPGDRL
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECHHHHHHCCCCCCCEE
YLVAKGLIRIGVLSADGREVTYGLIKPGQLFGEIAVLDGKERSADATAMEATELIALERK
EHEEHHHHHHEEECCCCCEEEEECCCCHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHH
DVHTFLHRHPAQALHLIEVLCERIRRADNQLEDMVFLSLPSRLAKHLLMLVQTMGTKAKP
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GTPSAIKLSQQEIADHLGISRESVNKVLSKWEQAGIVTLGRGQITLNKTAALEGLTSLE
CCCHHEEHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEECCCEEEECHHHHHCCHHCCC
>Mature Secondary Structure
MEKSSSMPETVLRRREMLANTPLFASVQTDLLDELAAKAKMVKVDARETLFSKGDPGDRL
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECHHHHHHCCCCCCCEE
YLVAKGLIRIGVLSADGREVTYGLIKPGQLFGEIAVLDGKERSADATAMEATELIALERK
EHEEHHHHHHEEECCCCCEEEEECCCCHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHH
DVHTFLHRHPAQALHLIEVLCERIRRADNQLEDMVFLSLPSRLAKHLLMLVQTMGTKAKP
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GTPSAIKLSQQEIADHLGISRESVNKVLSKWEQAGIVTLGRGQITLNKTAALEGLTSLE
CCCHHEEHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEECCCEEEECHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8366058; 8905231 [H]