Click here to switch to the map view.

The map label for this gene is purK [H]

Identifier: 83311300

GI number: 83311300

Start: 2397158

End: 2398267

Strand: Reverse

Name: purK [H]

Synonym: amb2201

Alternate gene names: 83311300

Gene position: 2398267-2397158 (Counterclockwise)

Preceding gene: 83311301

Following gene: 83311299

Centisome position: 48.28

GC content: 68.47

Gene sequence:

>1110_bases
GTGAGCCACGATCCCGCGTCGTCTCCGCTGCCGCCCGGCAGCACCATCGGCATTATCGGCGGCGGCCAGTTGGGCCGCAT
GGCCGCCCTGGCGGCGGCGCGGCTGGGCTATCGCGTCCACATCTTCACGCCCGAGACCGACTCTCCCGCCGCCCAGGTGA
GCGACGCGGCCACCATCGCCCGCTACGACGACCAGGAGGCCATCGAGCGCTTCGTGCGGTCCGTGGACGTGGTGACCTTC
GAGTTCGAGAATATTCCGGCGGAAAGCGTGCGCCACATGGCCGAGCACGTGCCGGTGCGCCCCTGCTGGAGCGTGCTGGA
AACGGCGCAGGACCGCTGCAACGAAAAGCGCTTCTTCAACTCCATCGGCATCGAGACCGCGCCCTGGCGGGCGGTGGGCA
GCCTGGACGACCTGCGCCAGGCCATCCAAGAAATCGGGCGCCCCGCCGTGCTGAAGACCGCCCGGCTGGGCTATGACGGC
AAGGGTCAGGTCAAGATTGGCCCTGACACCGATCCGGCCGAGGCCTGGGCCGCCTTGGGCACGGGTCTGGACCAGGCCGG
CCTGGGCGTGCTGGAAGGCTTCATCGACTTCCTGGGCGAGATCTCGGTGATCGTCGCGCGCGGCGCCGACGGCGAATGGG
CCGCCTTCGACCCGGTGTGGAACGTCCATCGCGACCACATCCTCGACACCACCACCGTTCCCGCCCCCATTTCGCCCTCC
CTCATGGATCAGGCCATGGACATCGCCCACCGCGCTGCCGAGGCGCTGGGCGTGGTCGGGCTGCTGGCCGTTGAGATGTT
CGTGACACCCAACGGGTTGCTGGCCAATGAAATGGCGCCCCGGCCGCATAATTCCGGCCACTGGACCATGGATGCCTGCG
TCACCGACCAGTTCGAGCAGTTCATCCGCGCCGTCTGCGGCCTGCCGCTGGGCAACCCGGAACGCCATTCGGACGCCGAG
ATGAAGAACCTGATCGGCGCCGATGCGGACCAGTGGCTGGACATCCTGAAGGACTCTTCGGCGCGGCTGCATCTCTACGG
CAAGGCCGAGGCCCGTCCTGGCCGCAAGATGGGCCACGTCAACCGGATCCGGCCCCGTTCCGACCGCTGA

Upstream 100 bases:

>100_bases
ACGAGGCCGTGGACAAGGCGCTGGAGGCCTGGCGCGCCCGCCAGACCGAGTCCGTGGCCGAGGCCCCGGTGGACCCCGAT
TCTCCCGACCTGATCCGCCC

Downstream 100 bases:

>100_bases
ACGGCCTATTCGCGATGACGATTTCGTAACCAAGCGATTTCGACGTTGACCCCCGGGCTTTATTGCCGGATTTTGACTCC
CGGTATTAGCCTTTAGATCT

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MSHDPASSPLPPGSTIGIIGGGQLGRMAALAAARLGYRVHIFTPETDSPAAQVSDAATIARYDDQEAIERFVRSVDVVTF
EFENIPAESVRHMAEHVPVRPCWSVLETAQDRCNEKRFFNSIGIETAPWRAVGSLDDLRQAIQEIGRPAVLKTARLGYDG
KGQVKIGPDTDPAEAWAALGTGLDQAGLGVLEGFIDFLGEISVIVARGADGEWAAFDPVWNVHRDHILDTTTVPAPISPS
LMDQAMDIAHRAAEALGVVGLLAVEMFVTPNGLLANEMAPRPHNSGHWTMDACVTDQFEQFIRAVCGLPLGNPERHSDAE
MKNLIGADADQWLDILKDSSARLHLYGKAEARPGRKMGHVNRIRPRSDR

Sequences:

>Translated_369_residues
MSHDPASSPLPPGSTIGIIGGGQLGRMAALAAARLGYRVHIFTPETDSPAAQVSDAATIARYDDQEAIERFVRSVDVVTF
EFENIPAESVRHMAEHVPVRPCWSVLETAQDRCNEKRFFNSIGIETAPWRAVGSLDDLRQAIQEIGRPAVLKTARLGYDG
KGQVKIGPDTDPAEAWAALGTGLDQAGLGVLEGFIDFLGEISVIVARGADGEWAAFDPVWNVHRDHILDTTTVPAPISPS
LMDQAMDIAHRAAEALGVVGLLAVEMFVTPNGLLANEMAPRPHNSGHWTMDACVTDQFEQFIRAVCGLPLGNPERHSDAE
MKNLIGADADQWLDILKDSSARLHLYGKAEARPGRKMGHVNRIRPRSDR
>Mature_368_residues
SHDPASSPLPPGSTIGIIGGGQLGRMAALAAARLGYRVHIFTPETDSPAAQVSDAATIARYDDQEAIERFVRSVDVVTFE
FENIPAESVRHMAEHVPVRPCWSVLETAQDRCNEKRFFNSIGIETAPWRAVGSLDDLRQAIQEIGRPAVLKTARLGYDGK
GQVKIGPDTDPAEAWAALGTGLDQAGLGVLEGFIDFLGEISVIVARGADGEWAAFDPVWNVHRDHILDTTTVPAPISPSL
MDQAMDIAHRAAEALGVVGLLAVEMFVTPNGLLANEMAPRPHNSGHWTMDACVTDQFEQFIRAVCGLPLGNPERHSDAEM
KNLIGADADQWLDILKDSSARLHLYGKAEARPGRKMGHVNRIRPRSDR

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=350, Percent_Identity=34.5714285714286, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1788155, Length=359, Percent_Identity=27.0194986072423, Blast_Score=120, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324702, Length=370, Percent_Identity=32.7027027027027, Blast_Score=176, Evalue=6e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 40029; Mature: 39897

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50975 ATP_GRASP ; PS00065 D_2_HYDROXYACID_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHDPASSPLPPGSTIGIIGGGQLGRMAALAAARLGYRVHIFTPETDSPAAQVSDAATIA
CCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHH
RYDDQEAIERFVRSVDVVTFEFENIPAESVRHMAEHVPVRPCWSVLETAQDRCNEKRFFN
HCCCHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
SIGIETAPWRAVGSLDDLRQAIQEIGRPAVLKTARLGYDGKGQVKIGPDTDPAEAWAALG
HCCCCCCCCHHHCCHHHHHHHHHHHCCCCEEHHHHCCCCCCCCEEECCCCCHHHHHHHHH
TGLDQAGLGVLEGFIDFLGEISVIVARGADGEWAAFDPVWNVHRDHILDTTTVPAPISPS
CCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCEECCCHHHCCHHHCCCCCCCCCCCCHH
LMDQAMDIAHRAAEALGVVGLLAVEMFVTPNGLLANEMAPRPHNSGHWTMDACVTDQFEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHH
FIRAVCGLPLGNPERHSDAEMKNLIGADADQWLDILKDSSARLHLYGKAEARPGRKMGHV
HHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCCCCCHHCCC
NRIRPRSDR
CCCCCCCCC
>Mature Secondary Structure 
SHDPASSPLPPGSTIGIIGGGQLGRMAALAAARLGYRVHIFTPETDSPAAQVSDAATIA
CCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHH
RYDDQEAIERFVRSVDVVTFEFENIPAESVRHMAEHVPVRPCWSVLETAQDRCNEKRFFN
HCCCHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
SIGIETAPWRAVGSLDDLRQAIQEIGRPAVLKTARLGYDGKGQVKIGPDTDPAEAWAALG
HCCCCCCCCHHHCCHHHHHHHHHHHCCCCEEHHHHCCCCCCCCEEECCCCCHHHHHHHHH
TGLDQAGLGVLEGFIDFLGEISVIVARGADGEWAAFDPVWNVHRDHILDTTTVPAPISPS
CCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCEECCCHHHCCHHHCCCCCCCCCCCCHH
LMDQAMDIAHRAAEALGVVGLLAVEMFVTPNGLLANEMAPRPHNSGHWTMDACVTDQFEQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHH
FIRAVCGLPLGNPERHSDAEMKNLIGADADQWLDILKDSSARLHLYGKAEARPGRKMGHV
HHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCCCCCHHCCC
NRIRPRSDR
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]