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The map label for this gene is ispB [H]

Identifier: 83311261

GI number: 83311261

Start: 2345246

End: 2346265

Strand: Reverse

Name: ispB [H]

Synonym: amb2162

Alternate gene names: 83311261

Gene position: 2346265-2345246 (Counterclockwise)

Preceding gene: 83311266

Following gene: 83311257

Centisome position: 47.24

GC content: 64.41

Gene sequence:

>1020_bases
GTGGCGATCGTGGTCAATTTCGAGGACGAACGCGGCAAGAAGGCGAAGAGCTTCGACGCCCTTGTCGCTCTGGTCAAGGA
CGACCTGCAGAAGGTCAACCATGCCATCGTTGACCGTATGCACAGCCCGGTCGAGATGATCCCCCAGCTGGCCGGCCACA
TCATCGCGGCAGGCGGCAAGCGCCTGCGCCCGGTGCTGACCCTGGCCTCGGCCAAGCTGGCCGGACATACGGGCGAACGC
CACATCAAGCTGGCCGCCTGCGTCGAGTTCATCCACACCGCCACCCTGCTTCACGACGACGTGGTCGACGAATCCGAGCT
GCGGCGCGGCCAGGCTTCGGCCAATGCCCTATTCGGCAACAAGCCCAGCGTGCTGGTCGGCGATTTTCTGTTCTCCCGCT
CGTTCGAGCTGATGGTCGAGGACGGATCGCTGTCGGTGCTGGCCATCCTGTCGCGCGCCTCCTCGGTCATCGCCGAGGGC
GAGGTGCTGCAGCTGATCACCGCCAACGACACCGAGACCAGCGAGGATTCCTATCTCGAGGTCATCCGCTCCAAGACCGC
CGCCCTGTTCGCCGCCGCCTGCCGCATCGGCGCGGTGGTGGCCGACCGGCCCAAGGTTGAGGAAGAGGCGCTGGAATCCT
ATGGCCTCAACCTGGGCATCGCCTTCCAGCTGATCGACGACGTGCTCGACTATTCCGCCAAGCAGGCCGTGCTGGGCAAG
ACGGTGGGCGACGATTTCCGCGAAGGTAAGATCACACTGCCGGTCATCCTGGCCTTCCGTCGCGGCGGTGACGAGGAAAG
AGCCTTCTGGCGCCGCTGCCTGGAAGAACTGGACCAGTCGGACGAGGATATCGGCCAGGCCATCAAGCTGATGGAAAAGC
ACGGCAGCCTGGCCGCCACCATCGCCCGCGCCCGCCATTACGGCGAAATCGCCCGCGACGCATTGGGCATCTTCCCCGAC
AGCCCGGTGAAGCAGGCCATGATCGACGTCATCGATTTCTGTATCGACCGGGCTTATTAG

Upstream 100 bases:

>100_bases
GCGATTAGGAATTGGCGATGTAAGCTTGACCCGCTTTCCCAGGCGACACTATGTTCGTCCCGCGGGTAGGCATGCACCGC
ACGGGAGGAGTTTCTGCATC

Downstream 100 bases:

>100_bases
CCCACCGCTTGGCGGGCTGCCGCCCGCGCCCGCCCGGGGCGGGAGCCCCGCATGCCCTACTCCGCCGCGAAGCGCGTCCG
GGGCGCCGGCAGCACCATGG

Product: geranylgeranyl pyrophosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MAIVVNFEDERGKKAKSFDALVALVKDDLQKVNHAIVDRMHSPVEMIPQLAGHIIAAGGKRLRPVLTLASAKLAGHTGER
HIKLAACVEFIHTATLLHDDVVDESELRRGQASANALFGNKPSVLVGDFLFSRSFELMVEDGSLSVLAILSRASSVIAEG
EVLQLITANDTETSEDSYLEVIRSKTAALFAAACRIGAVVADRPKVEEEALESYGLNLGIAFQLIDDVLDYSAKQAVLGK
TVGDDFREGKITLPVILAFRRGGDEERAFWRRCLEELDQSDEDIGQAIKLMEKHGSLAATIARARHYGEIARDALGIFPD
SPVKQAMIDVIDFCIDRAY

Sequences:

>Translated_339_residues
MAIVVNFEDERGKKAKSFDALVALVKDDLQKVNHAIVDRMHSPVEMIPQLAGHIIAAGGKRLRPVLTLASAKLAGHTGER
HIKLAACVEFIHTATLLHDDVVDESELRRGQASANALFGNKPSVLVGDFLFSRSFELMVEDGSLSVLAILSRASSVIAEG
EVLQLITANDTETSEDSYLEVIRSKTAALFAAACRIGAVVADRPKVEEEALESYGLNLGIAFQLIDDVLDYSAKQAVLGK
TVGDDFREGKITLPVILAFRRGGDEERAFWRRCLEELDQSDEDIGQAIKLMEKHGSLAATIARARHYGEIARDALGIFPD
SPVKQAMIDVIDFCIDRAY
>Mature_338_residues
AIVVNFEDERGKKAKSFDALVALVKDDLQKVNHAIVDRMHSPVEMIPQLAGHIIAAGGKRLRPVLTLASAKLAGHTGERH
IKLAACVEFIHTATLLHDDVVDESELRRGQASANALFGNKPSVLVGDFLFSRSFELMVEDGSLSVLAILSRASSVIAEGE
VLQLITANDTETSEDSYLEVIRSKTAALFAAACRIGAVVADRPKVEEEALESYGLNLGIAFQLIDDVLDYSAKQAVLGKT
VGDDFREGKITLPVILAFRRGGDEERAFWRRCLEELDQSDEDIGQAIKLMEKHGSLAATIARARHYGEIARDALGIFPDS
PVKQAMIDVIDFCIDRAY

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=286, Percent_Identity=31.1188811188811, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4758430, Length=207, Percent_Identity=24.6376811594203, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI83700220, Length=207, Percent_Identity=24.6376811594203, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1789578, Length=321, Percent_Identity=45.1713395638629, Blast_Score=284, Evalue=6e-78,
Organism=Escherichia coli, GI1786623, Length=242, Percent_Identity=33.0578512396694, Blast_Score=96, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17505681, Length=265, Percent_Identity=35.0943396226415, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6319475, Length=298, Percent_Identity=31.8791946308725, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6325188, Length=269, Percent_Identity=24.5353159851301, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24651612, Length=297, Percent_Identity=30.976430976431, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI281365769, Length=260, Percent_Identity=26.1538461538462, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24660002, Length=260, Percent_Identity=26.1538461538462, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36985; Mature: 36853

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIVVNFEDERGKKAKSFDALVALVKDDLQKVNHAIVDRMHSPVEMIPQLAGHIIAAGGK
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCC
RLRPVLTLASAKLAGHTGERHIKLAACVEFIHTATLLHDDVVDESELRRGQASANALFGN
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHCCC
KPSVLVGDFLFSRSFELMVEDGSLSVLAILSRASSVIAEGEVLQLITANDTETSEDSYLE
CCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHH
VIRSKTAALFAAACRIGAVVADRPKVEEEALESYGLNLGIAFQLIDDVLDYSAKQAVLGK
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
TVGDDFREGKITLPVILAFRRGGDEERAFWRRCLEELDQSDEDIGQAIKLMEKHGSLAAT
HHCCCHHCCCEEHHHHHEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHH
IARARHYGEIARDALGIFPDSPVKQAMIDVIDFCIDRAY
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIVVNFEDERGKKAKSFDALVALVKDDLQKVNHAIVDRMHSPVEMIPQLAGHIIAAGGK
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCC
RLRPVLTLASAKLAGHTGERHIKLAACVEFIHTATLLHDDVVDESELRRGQASANALFGN
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHCCC
KPSVLVGDFLFSRSFELMVEDGSLSVLAILSRASSVIAEGEVLQLITANDTETSEDSYLE
CCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHH
VIRSKTAALFAAACRIGAVVADRPKVEEEALESYGLNLGIAFQLIDDVLDYSAKQAVLGK
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
TVGDDFREGKITLPVILAFRRGGDEERAFWRRCLEELDQSDEDIGQAIKLMEKHGSLAAT
HHCCCHHCCCEEHHHHHEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHH
IARARHYGEIARDALGIFPDSPVKQAMIDVIDFCIDRAY
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]