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The map label for this gene is purF [H]

Identifier: 83311188

GI number: 83311188

Start: 2264968

End: 2266431

Strand: Reverse

Name: purF [H]

Synonym: amb2089

Alternate gene names: 83311188

Gene position: 2266431-2264968 (Counterclockwise)

Preceding gene: 83311189

Following gene: 83311187

Centisome position: 45.63

GC content: 66.6

Gene sequence:

>1464_bases
ATGCTGACCACCCACCCCTTCGACGACGACCATCTCCGCGAGGAATGCGGCGTGTTCGGCATTTTCGGCCATCCGGAAGC
CGCCGCCCACGTGGCCCTGGGCCTGCACGCCCTGCAGCATCGCGGCCAGGAGGCGGCGGGCATCGTCTCCTATGACGGCA
CCCAGTTCCACAACCACCGCGGCCTGGGTCATGTGGAGGACAATTTCGGGTCCGAGACGGTCATCCGCAAGCTGAAGGGC
TCCATGGCGGTGGGCCATGTGCGCTATTCCACCACGGGCGAGACCCTGCTGCGCAATGTGCAGCCGCTGTTCGCAGAGAT
GGAGTTCGGCGGTCTGGCGCTGGGCCACAACGGCAACCTGACCAATGCCATGGCGCTGCGCCGCCAGTTGGTGCGGCGCG
GCTGCCTGTTCCAGTCGACCACCGACACCGAGGTGATCATCCACCTGATCGCCATCAGCCTGTATTCCACGGTCGAGGAC
CGGATGATCGACGCGCTGCGTCAGATCGAGGGCGCCTATTCCCTGGTGGCCGCCACCACCAACGCGGTGTTCGGGGTGCG
TGATCCGCTGGGCATCCGGCCGCTCTGCCTGGGCCGGCTGGACAAGGCGTGGATCCTAGCCTCGGAATCCTGTGCCCTCG
ACATCATCGGCGCCGAATTCGTCCGCGACGTGGAGCCGGGCGAGATCGTGGTGCTGACCGCCGACGGCATCCGCTCGGTC
AAGCCGTTCACCAAGCGCCCGTCGCGCCTGTGCATCTTCGAATACATCTACTTCGCCCGCCCCGATTCGGTGGTGGAGGG
CACAAGCGTCTACGAGGCGCGCAAGCGGATCGGCAGCGAACTGGCCCGCGAAAGCCAGGTGGACGCCGACGTGGTGGTGC
CGGTGCCCGATTCCGGCGTGCCGGCGGCCATCGGCTTCGCGGCCGAGGCGGGTATTCCCTTCGAGCTGGGGATCATCCGC
AACCATTATGTGGGCCGGACCTTCATCCAGCCCACGGACAACATCCGCAATACCGGCGTCAAGATGAAGCACAACGCCAA
CCGGGCCTCCCTGGCCGGCAAGCGCGTCATCCTGGTCGACGATTCCATCGTGCGCGGCACCACCAGCCGCAAGATCGTCG
AAATGGTGCGCCAGGCCGGCGCCACCGAGGTGCACATGCGCATCTCCAGCCCGCCGACCACCCATTCGTGCTATTTCGGC
ATCGACACCCCCGAGCGCGAGAAGCTGCTGGCGGCGCGCTACGACGTGGACGGCATGGCCAAGCTGATCGGCGTGGATTC
CCTGGCCTTCATCTCCATCGACGGCCTGTACCGCGCCGTGGGCGAGCCCGGCCGCAACGCGGCCGAGCCGCAGTTCTGCG
ACGCCTGCTTCACCGGCGACTATCCGGTGGAGCCCACCGACTACATCAGCGCCGGCACCGATAACACCAAGCAGCTTTCC
CTGCTGACCGAGGACAGCCACTGA

Upstream 100 bases:

>100_bases
CTATGACGGCGAAAGCCGCACCCAGATGGAGCGGTTGATCCGCACCAATCAGTAATTCCCCGTCCCGACGCCCCCCGCAC
CGAATCCAGGACACAATCCC

Downstream 100 bases:

>100_bases
TGTCGGGACGCCTTGAGGGCCGCGTGGCCCTGGTCACCGGCGCCTCGCGCGGCATCGGAGCCGCCGTGGCCCGCCGCCTC
GCCGCCGAGGGGGCAGAAAT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MLTTHPFDDDHLREECGVFGIFGHPEAAAHVALGLHALQHRGQEAAGIVSYDGTQFHNHRGLGHVEDNFGSETVIRKLKG
SMAVGHVRYSTTGETLLRNVQPLFAEMEFGGLALGHNGNLTNAMALRRQLVRRGCLFQSTTDTEVIIHLIAISLYSTVED
RMIDALRQIEGAYSLVAATTNAVFGVRDPLGIRPLCLGRLDKAWILASESCALDIIGAEFVRDVEPGEIVVLTADGIRSV
KPFTKRPSRLCIFEYIYFARPDSVVEGTSVYEARKRIGSELARESQVDADVVVPVPDSGVPAAIGFAAEAGIPFELGIIR
NHYVGRTFIQPTDNIRNTGVKMKHNANRASLAGKRVILVDDSIVRGTTSRKIVEMVRQAGATEVHMRISSPPTTHSCYFG
IDTPEREKLLAARYDVDGMAKLIGVDSLAFISIDGLYRAVGEPGRNAAEPQFCDACFTGDYPVEPTDYISAGTDNTKQLS
LLTEDSH

Sequences:

>Translated_487_residues
MLTTHPFDDDHLREECGVFGIFGHPEAAAHVALGLHALQHRGQEAAGIVSYDGTQFHNHRGLGHVEDNFGSETVIRKLKG
SMAVGHVRYSTTGETLLRNVQPLFAEMEFGGLALGHNGNLTNAMALRRQLVRRGCLFQSTTDTEVIIHLIAISLYSTVED
RMIDALRQIEGAYSLVAATTNAVFGVRDPLGIRPLCLGRLDKAWILASESCALDIIGAEFVRDVEPGEIVVLTADGIRSV
KPFTKRPSRLCIFEYIYFARPDSVVEGTSVYEARKRIGSELARESQVDADVVVPVPDSGVPAAIGFAAEAGIPFELGIIR
NHYVGRTFIQPTDNIRNTGVKMKHNANRASLAGKRVILVDDSIVRGTTSRKIVEMVRQAGATEVHMRISSPPTTHSCYFG
IDTPEREKLLAARYDVDGMAKLIGVDSLAFISIDGLYRAVGEPGRNAAEPQFCDACFTGDYPVEPTDYISAGTDNTKQLS
LLTEDSH
>Mature_487_residues
MLTTHPFDDDHLREECGVFGIFGHPEAAAHVALGLHALQHRGQEAAGIVSYDGTQFHNHRGLGHVEDNFGSETVIRKLKG
SMAVGHVRYSTTGETLLRNVQPLFAEMEFGGLALGHNGNLTNAMALRRQLVRRGCLFQSTTDTEVIIHLIAISLYSTVED
RMIDALRQIEGAYSLVAATTNAVFGVRDPLGIRPLCLGRLDKAWILASESCALDIIGAEFVRDVEPGEIVVLTADGIRSV
KPFTKRPSRLCIFEYIYFARPDSVVEGTSVYEARKRIGSELARESQVDADVVVPVPDSGVPAAIGFAAEAGIPFELGIIR
NHYVGRTFIQPTDNIRNTGVKMKHNANRASLAGKRVILVDDSIVRGTTSRKIVEMVRQAGATEVHMRISSPPTTHSCYFG
IDTPEREKLLAARYDVDGMAKLIGVDSLAFISIDGLYRAVGEPGRNAAEPQFCDACFTGDYPVEPTDYISAGTDNTKQLS
LLTEDSH

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=507, Percent_Identity=38.6587771203156, Blast_Score=371, Evalue=1e-102,
Organism=Escherichia coli, GI1788651, Length=477, Percent_Identity=39.4129979035639, Blast_Score=311, Evalue=5e-86,
Organism=Escherichia coli, GI1790167, Length=239, Percent_Identity=30.9623430962343, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=478, Percent_Identity=40.1673640167364, Blast_Score=333, Evalue=9e-92,
Organism=Saccharomyces cerevisiae, GI6323958, Length=477, Percent_Identity=34.5911949685535, Blast_Score=252, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24659598, Length=488, Percent_Identity=44.0573770491803, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24659604, Length=466, Percent_Identity=44.6351931330472, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI28573187, Length=502, Percent_Identity=42.2310756972112, Blast_Score=373, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 53104; Mature: 53104

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTTHPFDDDHLREECGVFGIFGHPEAAAHVALGLHALQHRGQEAAGIVSYDGTQFHNHR
CCCCCCCCHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEECCCC
GLGHVEDNFGSETVIRKLKGSMAVGHVRYSTTGETLLRNVQPLFAEMEFGGLALGHNGNL
CCCCCCCCCCHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCCCCC
TNAMALRRQLVRRGCLFQSTTDTEVIIHLIAISLYSTVEDRMIDALRQIEGAYSLVAATT
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
NAVFGVRDPLGIRPLCLGRLDKAWILASESCALDIIGAEFVRDVEPGEIVVLTADGIRSV
CCEEECCCCCCCCHHHHCCCCCEEEEECCCCEEEEECHHHHHCCCCCCEEEEECCCCCCC
KPFTKRPSRLCIFEYIYFARPDSVVEGTSVYEARKRIGSELARESQVDADVVVPVPDSGV
CCHHCCCCCEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
PAAIGFAAEAGIPFELGIIRNHYVGRTFIQPTDNIRNTGVKMKHNANRASLAGKRVILVD
CCEEEHHHCCCCCEEEEEECCCCCCCEEECCCCCHHHCCEEEEECCCHHHHCCCEEEEEC
DSIVRGTTSRKIVEMVRQAGATEVHMRISSPPTTHSCYFGIDTPEREKLLAARYDVDGMA
CCHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCEEEECCCCCCHHHHHHHHCCHHHHH
KLIGVDSLAFISIDGLYRAVGEPGRNAAEPQFCDACFTGDYPVEPTDYISAGTDNTKQLS
HHHCCCCEEEEEEHHHHHHHCCCCCCCCCCCHHHHEECCCCCCCCCHHCCCCCCCCEEEE
LLTEDSH
EEECCCC
>Mature Secondary Structure
MLTTHPFDDDHLREECGVFGIFGHPEAAAHVALGLHALQHRGQEAAGIVSYDGTQFHNHR
CCCCCCCCHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEECCCC
GLGHVEDNFGSETVIRKLKGSMAVGHVRYSTTGETLLRNVQPLFAEMEFGGLALGHNGNL
CCCCCCCCCCHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCCCCC
TNAMALRRQLVRRGCLFQSTTDTEVIIHLIAISLYSTVEDRMIDALRQIEGAYSLVAATT
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
NAVFGVRDPLGIRPLCLGRLDKAWILASESCALDIIGAEFVRDVEPGEIVVLTADGIRSV
CCEEECCCCCCCCHHHHCCCCCEEEEECCCCEEEEECHHHHHCCCCCCEEEEECCCCCCC
KPFTKRPSRLCIFEYIYFARPDSVVEGTSVYEARKRIGSELARESQVDADVVVPVPDSGV
CCHHCCCCCEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
PAAIGFAAEAGIPFELGIIRNHYVGRTFIQPTDNIRNTGVKMKHNANRASLAGKRVILVD
CCEEEHHHCCCCCEEEEEECCCCCCCEEECCCCCHHHCCEEEEECCCHHHHCCCEEEEEC
DSIVRGTTSRKIVEMVRQAGATEVHMRISSPPTTHSCYFGIDTPEREKLLAARYDVDGMA
CCHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCEEEECCCCCCHHHHHHHHCCHHHHH
KLIGVDSLAFISIDGLYRAVGEPGRNAAEPQFCDACFTGDYPVEPTDYISAGTDNTKQLS
HHHCCCCEEEEEEHHHHHHHCCCCCCCCCCCHHHHEECCCCCCCCCHHCCCCCCCCEEEE
LLTEDSH
EEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA