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The map label for this gene is appA [H]

Identifier: 83311078

GI number: 83311078

Start: 2137078

End: 2138490

Strand: Reverse

Name: appA [H]

Synonym: amb1979

Alternate gene names: 83311078

Gene position: 2138490-2137078 (Counterclockwise)

Preceding gene: 83311079

Following gene: 83311077

Centisome position: 43.05

GC content: 66.81

Gene sequence:

>1413_bases
TTGGCCAAGGTGGAGACCCTGGCGGGCGGCAAGAGGGGCATCGCCGCCACCTACACGCTGCGGGCCGACGCCCGCTGGGC
CGACGGCAAGCCAGTGACCAGCGACGACGTGGTGTTCAGTTGGGAGGTGGGGCGCCATCCCCAGTCGGGCGTGGCGGCGG
CCGAGCTGTTCCGCCGCATCACCAGGGTCGAGGTCAAGGACGCCCGCACCTTCACCCTGCACGTGGACAAGCTGACCTTC
GACTACAACGCCATCAACGATCTGCGGCTGCTGCCCGCCCATATCGAGGGGCCGCGCTTCAAAAGCGCCCCCGCCGAGTA
CCGCAACCGCACCGCCTACGACCAGGATTCCACCAATCCCGGCCTCTATAACGGCCCCTACCGCATCACTTCGGTGGTCC
AGGGCAGCCACGTGGTGCTGGAGCCCAATGCCCATTGGGGCGGCGCCAAGCCCGCCTTCAGGCGCATTGTCGTCAAGGCC
ATCGAGAACACCGCCTCGCTGGAGGCCAACCTGCTGTCGGGCGGCATCGACATGATCGCCGGCGAGTTGGGCCTGCCGCT
GGAGCAGTCCCTGGCCTTCGAGAAGCGCCACGGCGCCCGCTTTGCCGTCATCACCAAGCCCAGCCTGACCTACGAGCATC
TGGATATCAACCTGGGCTCGCCCATCCTGGCCGTCCCCGAAATCCGCCGCGCCCTGCTGCTGGGCCTGGATCGCGAGACC
ATCTCGCGCCAATTGTTCGAGGGCCGCCAGATCGTCGCCGAATCCCTGGTGCCGCCGCTGGACTGGGTGTTCGCCGCCGA
TCTGCCCAAGGCGACCTTCGATCCGGCCCGCGCCGCCGTCCTGCTGGATCAGGCGGGGTGGAAACCGGGGCCCGGCGGCA
TCCGGGTCAACGCCAAGGGCGAGCGCCTGTCGCTGGAACTGGTCACCACCGCCGGCAACCGCTCACGCGAGCTGGTGGCG
CAGGTGATGCAATCCCAGTGGAAGAAGATCGGCGTCGAGCTTCGTCTGAAGACCGAGCCGCCCCGCGTGCTGTTCGGTGA
CACGGTGACCCGGCGCAAATTCCCCCACATGGCGCTGTTCGCCTGGTATTCGGCCCCGGAAAGCGTGCCGCGCTCGATGC
TCCATTCCAGCATGATCCCCAATGGAGCCAACAACTGGTCGGGACAGAATTACACCGGCTACGCCAGCCCGGCCATGGAC
AGGCTGATCGACGCGGTCGAGGTGGAGCCGGACAAGGACAAGCGCAGGCTTCTGTGGCGGGAGATTCAGGCGCTCTATGC
CGCCGACCTGCCCGCCTTGCCACTGTTCTTCAGATCGGATTCCTTCATCCTGCCCAAAGGACTGAAGGGGCTGGAACCCA
CCGGCCACCAGGACCCCTCTCCCTATTGGGTGGAGAACTGGCGGTGGGAGTGA

Upstream 100 bases:

>100_bases
CTATGTGCTGGCCTTTGCCCATCGCCCCATCACCGCCTATGACGCGTCGTGGCAGCTGACCTGCCTGCTGTGCGAGACGC
TGCCCACCATCGAGAACGGC

Downstream 100 bases:

>100_bases
CCGGAAGGCTGATCCAGTCGGTCCTCGTGCTGCTGGTCATGTCCTTCGTGGTCTACGGCCTGATGGGCCTGATGCCCGGC
GACCCCATCGACCTGATGAT

Product: ABC-type dipeptide transport system

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MAKVETLAGGKRGIAATYTLRADARWADGKPVTSDDVVFSWEVGRHPQSGVAAAELFRRITRVEVKDARTFTLHVDKLTF
DYNAINDLRLLPAHIEGPRFKSAPAEYRNRTAYDQDSTNPGLYNGPYRITSVVQGSHVVLEPNAHWGGAKPAFRRIVVKA
IENTASLEANLLSGGIDMIAGELGLPLEQSLAFEKRHGARFAVITKPSLTYEHLDINLGSPILAVPEIRRALLLGLDRET
ISRQLFEGRQIVAESLVPPLDWVFAADLPKATFDPARAAVLLDQAGWKPGPGGIRVNAKGERLSLELVTTAGNRSRELVA
QVMQSQWKKIGVELRLKTEPPRVLFGDTVTRRKFPHMALFAWYSAPESVPRSMLHSSMIPNGANNWSGQNYTGYASPAMD
RLIDAVEVEPDKDKRRLLWREIQALYAADLPALPLFFRSDSFILPKGLKGLEPTGHQDPSPYWVENWRWE

Sequences:

>Translated_470_residues
MAKVETLAGGKRGIAATYTLRADARWADGKPVTSDDVVFSWEVGRHPQSGVAAAELFRRITRVEVKDARTFTLHVDKLTF
DYNAINDLRLLPAHIEGPRFKSAPAEYRNRTAYDQDSTNPGLYNGPYRITSVVQGSHVVLEPNAHWGGAKPAFRRIVVKA
IENTASLEANLLSGGIDMIAGELGLPLEQSLAFEKRHGARFAVITKPSLTYEHLDINLGSPILAVPEIRRALLLGLDRET
ISRQLFEGRQIVAESLVPPLDWVFAADLPKATFDPARAAVLLDQAGWKPGPGGIRVNAKGERLSLELVTTAGNRSRELVA
QVMQSQWKKIGVELRLKTEPPRVLFGDTVTRRKFPHMALFAWYSAPESVPRSMLHSSMIPNGANNWSGQNYTGYASPAMD
RLIDAVEVEPDKDKRRLLWREIQALYAADLPALPLFFRSDSFILPKGLKGLEPTGHQDPSPYWVENWRWE
>Mature_469_residues
AKVETLAGGKRGIAATYTLRADARWADGKPVTSDDVVFSWEVGRHPQSGVAAAELFRRITRVEVKDARTFTLHVDKLTFD
YNAINDLRLLPAHIEGPRFKSAPAEYRNRTAYDQDSTNPGLYNGPYRITSVVQGSHVVLEPNAHWGGAKPAFRRIVVKAI
ENTASLEANLLSGGIDMIAGELGLPLEQSLAFEKRHGARFAVITKPSLTYEHLDINLGSPILAVPEIRRALLLGLDRETI
SRQLFEGRQIVAESLVPPLDWVFAADLPKATFDPARAAVLLDQAGWKPGPGGIRVNAKGERLSLELVTTAGNRSRELVAQ
VMQSQWKKIGVELRLKTEPPRVLFGDTVTRRKFPHMALFAWYSAPESVPRSMLHSSMIPNGANNWSGQNYTGYASPAMDR
LIDAVEVEPDKDKRRLLWREIQALYAADLPALPLFFRSDSFILPKGLKGLEPTGHQDPSPYWVENWRWE

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787762, Length=437, Percent_Identity=24.9427917620137, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789887, Length=426, Percent_Identity=23.4741784037559, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789966, Length=446, Percent_Identity=21.52466367713, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 52324; Mature: 52193

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVETLAGGKRGIAATYTLRADARWADGKPVTSDDVVFSWEVGRHPQSGVAAAELFRRI
CCCCCCCCCCCCCEEEEEEEEECCEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHH
TRVEVKDARTFTLHVDKLTFDYNAINDLRLLPAHIEGPRFKSAPAEYRNRTAYDQDSTNP
HHEEECCCEEEEEEEEEEEECCCCCCCCEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCC
GLYNGPYRITSVVQGSHVVLEPNAHWGGAKPAFRRIVVKAIENTASLEANLLSGGIDMIA
CCCCCCEEEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
GELGLPLEQSLAFEKRHGARFAVITKPSLTYEHLDINLGSPILAVPEIRRALLLGLDRET
HHCCCCHHHHHHHHHHCCCEEEEEECCCCEEEEEEEECCCCEEEHHHHHHHHHHCCCHHH
ISRQLFEGRQIVAESLVPPLDWVFAADLPKATFDPARAAVLLDQAGWKPGPGGIRVNAKG
HHHHHHHHHHHHHHHCCCCHHHHEECCCCCCCCCCHHEEEEECCCCCCCCCCCEEECCCC
ERLSLELVTTAGNRSRELVAQVMQSQWKKIGVELRLKTEPPRVLFGDTVTRRKFPHMALF
CEEEEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCEEECCCHHHHCCCCEEEE
AWYSAPESVPRSMLHSSMIPNGANNWSGQNYTGYASPAMDRLIDAVEVEPDKDKRRLLWR
EECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
EIQALYAADLPALPLFFRSDSFILPKGLKGLEPTGHQDPSPYWVENWRWE
HHHHHHHCCCCCCEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKVETLAGGKRGIAATYTLRADARWADGKPVTSDDVVFSWEVGRHPQSGVAAAELFRRI
CCCCCCCCCCCCEEEEEEEEECCEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHH
TRVEVKDARTFTLHVDKLTFDYNAINDLRLLPAHIEGPRFKSAPAEYRNRTAYDQDSTNP
HHEEECCCEEEEEEEEEEEECCCCCCCCEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCC
GLYNGPYRITSVVQGSHVVLEPNAHWGGAKPAFRRIVVKAIENTASLEANLLSGGIDMIA
CCCCCCEEEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
GELGLPLEQSLAFEKRHGARFAVITKPSLTYEHLDINLGSPILAVPEIRRALLLGLDRET
HHCCCCHHHHHHHHHHCCCEEEEEECCCCEEEEEEEECCCCEEEHHHHHHHHHHCCCHHH
ISRQLFEGRQIVAESLVPPLDWVFAADLPKATFDPARAAVLLDQAGWKPGPGGIRVNAKG
HHHHHHHHHHHHHHHCCCCHHHHEECCCCCCCCCCHHEEEEECCCCCCCCCCCEEECCCC
ERLSLELVTTAGNRSRELVAQVMQSQWKKIGVELRLKTEPPRVLFGDTVTRRKFPHMALF
CEEEEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCEEECCCHHHHCCCCEEEE
AWYSAPESVPRSMLHSSMIPNGANNWSGQNYTGYASPAMDRLIDAVEVEPDKDKRRLLWR
EECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
EIQALYAADLPALPLFFRSDSFILPKGLKGLEPTGHQDPSPYWVENWRWE
HHHHHHHCCCCCCEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]