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The map label for this gene is appB [H]

Identifier: 83311077

GI number: 83311077

Start: 2136140

End: 2137081

Strand: Reverse

Name: appB [H]

Synonym: amb1978

Alternate gene names: 83311077

Gene position: 2137081-2136140 (Counterclockwise)

Preceding gene: 83311078

Following gene: 83311076

Centisome position: 43.02

GC content: 69.32

Gene sequence:

>942_bases
GTGACCGGAAGGCTGATCCAGTCGGTCCTCGTGCTGCTGGTCATGTCCTTCGTGGTCTACGGCCTGATGGGCCTGATGCC
CGGCGACCCCATCGACCTGATGATCGCCGGCGATCCCCGCCTGACGCCCGAAGACGCCGTGCGGCTGAAAGTCCTTTACG
GCCTGGACCGCCCCTGGACCGAGCGCTGGCTGCGCTGGCTGGGGCAAGTGCTCCAGGGCGAGCTGGGCTATTCCCGCCTC
TATGCCCGTCCGGTTCTCGACGCCATGGCTCCGGCCCTGGGCAACAGCGCCCTGCTGATGCTGTCGGCCATGGCGCTGTC
CCTGTCCCTGGCCATTCCGCTGGGCATTCTCGCCTCGCTGCGACCCGGCGGCCTGGTGGACCGGCTGGTCAACCTCGCGG
CCTTCGCCTCGGTTTCCGTGCCGGTGTTCTGGCTGGGGCTGATGCTGATCGTGGTGTTCGCCGTGTCGCTGGGCTGGCTG
CCGGCCGGCGGGGTGGAGAGCGTGGGCGACGGCGGCCCCACCGACCGGCTGCGCCATATGGTGCTGCCCGTCTCCGCCCT
GGCCCTGGCCGGCATCGGACAGGCGACCCGGCACATGCGGGCGGCCATGATCGGCGAGGCGGGGGCCGATTACATCCGCA
CGGCGCGGGCCAAGGGCTGCGGTCCGGCCCGCATCGTGCTGCGGCACCAGTTGCGCAATGCCCTGCTGCCCATCACCACC
ATCATCGCCCTGGAATTCGGAGGCCTGTTCTCCGGCGCGCTGATCACCGAGACGGTGTTCGCCTGGCCGGGCATGGGCCG
GCTGATCTATGAAGCGGTGATGGGCAACGACTTCAATCTGGCCCTGTCGGGCCTGCTGCTGGCCACGGCCATGACCCTGG
CAGGCTCGATCCTGGCCGACATGGCCTATCAGCGCCTTGACCCCAGGATCGGCAAGCCATGA

Upstream 100 bases:

>100_bases
TCAGATCGGATTCCTTCATCCTGCCCAAAGGACTGAAGGGGCTGGAACCCACCGGCCACCAGGACCCCTCTCCCTATTGG
GTGGAGAACTGGCGGTGGGA

Downstream 100 bases:

>100_bases
AGGCCGGGGCCGCCATCCTGTTGCTGTTGCTGGCCGCGATTCTGGCGGCGCCCTGGCTGCTGCCGGCTCCCGAAGCGCTG
GATCTGGCCAGCCGCTTCGA

Product: ABC-type dipeptide/oligopeptide/nickel transport systems

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MTGRLIQSVLVLLVMSFVVYGLMGLMPGDPIDLMIAGDPRLTPEDAVRLKVLYGLDRPWTERWLRWLGQVLQGELGYSRL
YARPVLDAMAPALGNSALLMLSAMALSLSLAIPLGILASLRPGGLVDRLVNLAAFASVSVPVFWLGLMLIVVFAVSLGWL
PAGGVESVGDGGPTDRLRHMVLPVSALALAGIGQATRHMRAAMIGEAGADYIRTARAKGCGPARIVLRHQLRNALLPITT
IIALEFGGLFSGALITETVFAWPGMGRLIYEAVMGNDFNLALSGLLLATAMTLAGSILADMAYQRLDPRIGKP

Sequences:

>Translated_313_residues
MTGRLIQSVLVLLVMSFVVYGLMGLMPGDPIDLMIAGDPRLTPEDAVRLKVLYGLDRPWTERWLRWLGQVLQGELGYSRL
YARPVLDAMAPALGNSALLMLSAMALSLSLAIPLGILASLRPGGLVDRLVNLAAFASVSVPVFWLGLMLIVVFAVSLGWL
PAGGVESVGDGGPTDRLRHMVLPVSALALAGIGQATRHMRAAMIGEAGADYIRTARAKGCGPARIVLRHQLRNALLPITT
IIALEFGGLFSGALITETVFAWPGMGRLIYEAVMGNDFNLALSGLLLATAMTLAGSILADMAYQRLDPRIGKP
>Mature_312_residues
TGRLIQSVLVLLVMSFVVYGLMGLMPGDPIDLMIAGDPRLTPEDAVRLKVLYGLDRPWTERWLRWLGQVLQGELGYSRLY
ARPVLDAMAPALGNSALLMLSAMALSLSLAIPLGILASLRPGGLVDRLVNLAAFASVSVPVFWLGLMLIVVFAVSLGWLP
AGGVESVGDGGPTDRLRHMVLPVSALALAGIGQATRHMRAAMIGEAGADYIRTARAKGCGPARIVLRHQLRNALLPITTI
IALEFGGLFSGALITETVFAWPGMGRLIYEAVMGNDFNLALSGLLLATAMTLAGSILADMAYQRLDPRIGKP

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789965, Length=318, Percent_Identity=32.7044025157233, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1787053, Length=307, Percent_Identity=32.2475570032573, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI1787497, Length=308, Percent_Identity=32.7922077922078, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1789888, Length=310, Percent_Identity=32.5806451612903, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1787761, Length=324, Percent_Identity=34.2592592592593, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1787550, Length=261, Percent_Identity=27.9693486590038, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1788504, Length=292, Percent_Identity=30.4794520547945, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33337; Mature: 33206

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGRLIQSVLVLLVMSFVVYGLMGLMPGDPIDLMIAGDPRLTPEDAVRLKVLYGLDRPWT
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCHH
ERWLRWLGQVLQGELGYSRLYARPVLDAMAPALGNSALLMLSAMALSLSLAIPLGILASL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
RPGGLVDRLVNLAAFASVSVPVFWLGLMLIVVFAVSLGWLPAGGVESVGDGGPTDRLRHM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
VLPVSALALAGIGQATRHMRAAMIGEAGADYIRTARAKGCGPARIVLRHQLRNALLPITT
HHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
IIALEFGGLFSGALITETVFAWPGMGRLIYEAVMGNDFNLALSGLLLATAMTLAGSILAD
HHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
MAYQRLDPRIGKP
HHHHHHCCCCCCC
>Mature Secondary Structure 
TGRLIQSVLVLLVMSFVVYGLMGLMPGDPIDLMIAGDPRLTPEDAVRLKVLYGLDRPWT
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCHH
ERWLRWLGQVLQGELGYSRLYARPVLDAMAPALGNSALLMLSAMALSLSLAIPLGILASL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
RPGGLVDRLVNLAAFASVSVPVFWLGLMLIVVFAVSLGWLPAGGVESVGDGGPTDRLRHM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
VLPVSALALAGIGQATRHMRAAMIGEAGADYIRTARAKGCGPARIVLRHQLRNALLPITT
HHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
IIALEFGGLFSGALITETVFAWPGMGRLIYEAVMGNDFNLALSGLLLATAMTLAGSILAD
HHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
MAYQRLDPRIGKP
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]