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The map label for this gene is amtB [C]

Identifier: 83311068

GI number: 83311068

Start: 2126706

End: 2128046

Strand: Reverse

Name: amtB [C]

Synonym: amb1969

Alternate gene names: 83311068

Gene position: 2128046-2126706 (Counterclockwise)

Preceding gene: 83311071

Following gene: 83311057

Centisome position: 42.84

GC content: 65.92

Gene sequence:

>1341_bases
ATGCATCTTGAAGCCGCGCCGCCATCCCGCGGCGGCGGCAGCATTTTTGGGCAGAGGAATCCAATGGAGACCACGAAGAC
AGGCGCAGATGTATTGTTCATCCTCTTGGGGGCGGTGATGGTTCTGGCCATGCACTCCGGCTTTGCCTTCCTCGAGGTGG
GAACCGTCAGGAAAAAGAATCAGGTCAACGCCCTGGTCAAGATCCTGTCGGATTTCGCGGTCTCGACCATCGTCTATTTC
TTCGTGGGCTATTCCGTGGCCTACGGCACCACCTTCCTGGTGGGCGCCGACGTGCTGATCGGCAAGACCGGCAGCGCCTT
CGCCGCCTCGGGCTATGACCTGGTCAAGTTCTTCTTCCTGGCCACCTTCGCCGCCGCCATTCCCGCCATCGTGTCGGGCG
GCATCGCCGAGCGTGCCAAGTTCACCACCCAGCTGATGGCCACCGCCGTGCTGGTGGGGCTGGTCTATCCCCTGTTCGAG
GGCATGGTGTGGGGCACCCGCTTCGGCTTTCAGGACCTGATGAAGGGCTGGTTCGGCTTCGCCTTCCATGACTTCGCCGG
TTCGGTGGTGGTGCATGCGGTGGGCGGCTGGATCGGTCTGGCCGCCGTGCTGATGCTGGGCGTGCGCCTGGGCCGTTACC
GCAAGGACGGACGGCTGGTGGCCTTCCCCCCCTCCAACATCCCCTTCCTGGCGCTGGGCGCCTGGGTTCTGTCGGTGGGC
TGGTTCGGCTTCAACGTCATGAGCGCCCAGGTGATCGCCGACATTTCCGGGCTGGTGGCCATCAACTCGCTGATGGCCAT
GGTGGGCGGCATCGTCACCAGCCTGCTGGTGGGCCGCAACGACCCCGGCTTCATCCACAACGGCGCCCTGGCCGGTCTGG
TGGCGGTCTGCGCCGGGTCCGACGTCATGCATCCCGTGGGCGCCCTGGTCACTGGCGGCATCGCCGGCGCCATGTTCGTC
TGGCTGTTCAACCTGTGCCAAGCCAAATTCAAGATCGACGACGTTCTGGGTGTCTGGGCGCTGCACGGCATGTGCGGCTT
CCTGGGCGGCATCGCCTGCGGCGTGTTCGGTCAGGAAGCCCTGGGCGGCCTAGGCGGCGTCAGCCTGGGCGCCCAGGTCA
CCGGGACGGTCATGGGCGCCGCCTTTGGCCTGGCCGGTGGCCTGGCGGTCTATGGCGGCCTCAAGGCCACCCTGGGCATC
CGCCTCAGCGATGAGGAGGAATTCGCCGGTGCCGACCTGTCCATCCACCAGATCGGCGCCTATCCCGAAGACGCCACCAC
CATGCACGACGACGGCCTGGCCATGTCGGTGCACTCGGCCAACAAGCGCAAGGCCGGCTGA

Upstream 100 bases:

>100_bases
CATCGGCAGCGCGGATATTAAGCACAGCTCTGGCGCCTATTTTTTCCGCACACGACAATGCATTGTTTTTTCAAGATATT
GCCCAAAGCGGGCACTTGGC

Downstream 100 bases:

>100_bases
AAGCAACGCCCCTGAACCTTCAAGGGCCGCCCGGATCGGGCGGCCCTTTTTCTATTCCGTCACCACGCAGGAGGCGGGGA
AGGTCAGCATCACCTTGGTC

Product: ammonia permease

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MHLEAAPPSRGGGSIFGQRNPMETTKTGADVLFILLGAVMVLAMHSGFAFLEVGTVRKKNQVNALVKILSDFAVSTIVYF
FVGYSVAYGTTFLVGADVLIGKTGSAFAASGYDLVKFFFLATFAAAIPAIVSGGIAERAKFTTQLMATAVLVGLVYPLFE
GMVWGTRFGFQDLMKGWFGFAFHDFAGSVVVHAVGGWIGLAAVLMLGVRLGRYRKDGRLVAFPPSNIPFLALGAWVLSVG
WFGFNVMSAQVIADISGLVAINSLMAMVGGIVTSLLVGRNDPGFIHNGALAGLVAVCAGSDVMHPVGALVTGGIAGAMFV
WLFNLCQAKFKIDDVLGVWALHGMCGFLGGIACGVFGQEALGGLGGVSLGAQVTGTVMGAAFGLAGGLAVYGGLKATLGI
RLSDEEEFAGADLSIHQIGAYPEDATTMHDDGLAMSVHSANKRKAG

Sequences:

>Translated_446_residues
MHLEAAPPSRGGGSIFGQRNPMETTKTGADVLFILLGAVMVLAMHSGFAFLEVGTVRKKNQVNALVKILSDFAVSTIVYF
FVGYSVAYGTTFLVGADVLIGKTGSAFAASGYDLVKFFFLATFAAAIPAIVSGGIAERAKFTTQLMATAVLVGLVYPLFE
GMVWGTRFGFQDLMKGWFGFAFHDFAGSVVVHAVGGWIGLAAVLMLGVRLGRYRKDGRLVAFPPSNIPFLALGAWVLSVG
WFGFNVMSAQVIADISGLVAINSLMAMVGGIVTSLLVGRNDPGFIHNGALAGLVAVCAGSDVMHPVGALVTGGIAGAMFV
WLFNLCQAKFKIDDVLGVWALHGMCGFLGGIACGVFGQEALGGLGGVSLGAQVTGTVMGAAFGLAGGLAVYGGLKATLGI
RLSDEEEFAGADLSIHQIGAYPEDATTMHDDGLAMSVHSANKRKAG
>Mature_446_residues
MHLEAAPPSRGGGSIFGQRNPMETTKTGADVLFILLGAVMVLAMHSGFAFLEVGTVRKKNQVNALVKILSDFAVSTIVYF
FVGYSVAYGTTFLVGADVLIGKTGSAFAASGYDLVKFFFLATFAAAIPAIVSGGIAERAKFTTQLMATAVLVGLVYPLFE
GMVWGTRFGFQDLMKGWFGFAFHDFAGSVVVHAVGGWIGLAAVLMLGVRLGRYRKDGRLVAFPPSNIPFLALGAWVLSVG
WFGFNVMSAQVIADISGLVAINSLMAMVGGIVTSLLVGRNDPGFIHNGALAGLVAVCAGSDVMHPVGALVTGGIAGAMFV
WLFNLCQAKFKIDDVLGVWALHGMCGFLGGIACGVFGQEALGGLGGVSLGAQVTGTVMGAAFGLAGGLAVYGGLKATLGI
RLSDEEEFAGADLSIHQIGAYPEDATTMHDDGLAMSVHSANKRKAG

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Homo sapiens, GI156627565, Length=239, Percent_Identity=30.9623430962343, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1786656, Length=413, Percent_Identity=30.5084745762712, Blast_Score=147, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17550180, Length=425, Percent_Identity=30.3529411764706, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17550182, Length=468, Percent_Identity=29.4871794871795, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI32564042, Length=422, Percent_Identity=28.6729857819905, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI133931100, Length=386, Percent_Identity=25.9067357512953, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321559, Length=454, Percent_Identity=25.7709251101322, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6325396, Length=379, Percent_Identity=26.9129287598945, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324187, Length=424, Percent_Identity=26.8867924528302, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI161078314, Length=417, Percent_Identity=30.2158273381295, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 46171; Mature: 46171

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLEAAPPSRGGGSIFGQRNPMETTKTGADVLFILLGAVMVLAMHSGFAFLEVGTVRKKN
CCCCCCCCCCCCCCCCCCCCCCCCHHCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHH
QVNALVKILSDFAVSTIVYFFVGYSVAYGTTFLVGADVLIGKTGSAFAASGYDLVKFFFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEECCCCCHHCCCHHHHHHHHH
ATFAAAIPAIVSGGIAERAKFTTQLMATAVLVGLVYPLFEGMVWGTRFGFQDLMKGWFGF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHH
AFHDFAGSVVVHAVGGWIGLAAVLMLGVRLGRYRKDGRLVAFPPSNIPFLALGAWVLSVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHH
WFGFNVMSAQVIADISGLVAINSLMAMVGGIVTSLLVGRNDPGFIHNGALAGLVAVCAGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHCCC
DVMHPVGALVTGGIAGAMFVWLFNLCQAKFKIDDVLGVWALHGMCGFLGGIACGVFGQEA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LGGLGGVSLGAQVTGTVMGAAFGLAGGLAVYGGLKATLGIRLSDEEEFAGADLSIHQIGA
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEECCCHHHCCCCCEEHCCCC
YPEDATTMHDDGLAMSVHSANKRKAG
CCCCCCCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure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CCCCCCCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]