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The map label for this gene is hybA [H]

Identifier: 83310748

GI number: 83310748

Start: 1786589

End: 1787608

Strand: Reverse

Name: hybA [H]

Synonym: amb1649

Alternate gene names: 83310748

Gene position: 1787608-1786589 (Counterclockwise)

Preceding gene: 83310749

Following gene: 83310747

Centisome position: 35.99

GC content: 66.96

Gene sequence:

>1020_bases
ATGACCATCACCAGACGCAATTTCCTGGCGACGGCGGCGGCGGGCGGAACGGCGGCAGCGGCCGCCTGCGCCACCCTGGC
CCCCGCCGGCGCCGAGGCGCGGGAAAGCCATAAGGTTCCCGACAACGCCGTCGGCCTGCTCTACGACGCGACGCTGTGCA
TCGGCTGCAAGGCCTGCATGTCGGCGTGCAAGGAGGCCAACGACCTGCCGCTGGAAGACACCATCGGCGAAAAGCTGTGG
GACACGCCCCTCGAACTGTCGGGCAAGACCTACAACGTCATCAAGGTCTACCAGTCGGGCACCATGGAGAACAAGGACAA
GGCCGAGAACGGCTTTGCCCATATCAAGCGCTCGTGCCTGCACTGCGCCGACCCGTCCTGCGTGTCCGCCTGCCCGGTTT
CGGCCATGCAGAAGCGGGCCACCGACGGCGTCGTCACCTACAACAAGGACGCCTGCATCGGCTGCCGCTATTGCGTCGCC
GCCTGCCCCTTCGGCGTGCCCCAGTTCCAGTACGACACGCCCAAGCCGGAAATCGCCAAGTGCCAACTGTGCAAGCACAG
GATGGAACAGGGCAAGTATGCCGCCTGCGCGGAAAGCTGCCCCACCGGCGCCACCATTTTCGGCTCCTACGCCGCGCTGT
CGGCCGAGATCGACCGCCGCCGCGCCATGAAGCCGGGTGAGCCCAACCTGTTCCCCCGCCGCACCCCCGAATCGGGCGAC
ATGCACGAAAAGGCCGCGCCGGAATACGTGGACTATGTCTACGGCCAGAAGGACGCGGGCGGCACCCAGGTGCGCTACCT
GTCCGGGGTGGCCTTCGACAAGCTGGCCCTGCCCATGGGCCTGCCGGAACGGGCCTATGCCGCCGATTCCGAGACCCTGC
AGCACACCCTGTATGGCGGGATGATCCTGCCGGTGGTGGCGCTGGCCGGTCTGGTCACCGCCGCCTGGCGGGGCTCCAAG
GACCACCACGACGACGATGAGTTCGACATGTTCGACCATCCGGAAGGGGGGCACAAGTAA

Upstream 100 bases:

>100_bases
GGCGGTCGGTGGCCTGGCCATCGGTGCCGGCGCCAAGCTGGTGGGCAATCTGGGCAAGAGTGAAAACGGCCAGGATTCCG
GGAAACAGGGGTAATCCGCC

Downstream 100 bases:

>100_bases
TGGTCAATCACCCCATCCCCAGCCAGGTGATCAGTCGCCTGAAAAAGGGTAAGGCCAGCCCCACCGCCATCGGCGGCTCG
CTGGTCACCCCCTTCACCAT

Product: hydrogenase 2 protein HybA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MTITRRNFLATAAAGGTAAAAACATLAPAGAEARESHKVPDNAVGLLYDATLCIGCKACMSACKEANDLPLEDTIGEKLW
DTPLELSGKTYNVIKVYQSGTMENKDKAENGFAHIKRSCLHCADPSCVSACPVSAMQKRATDGVVTYNKDACIGCRYCVA
ACPFGVPQFQYDTPKPEIAKCQLCKHRMEQGKYAACAESCPTGATIFGSYAALSAEIDRRRAMKPGEPNLFPRRTPESGD
MHEKAAPEYVDYVYGQKDAGGTQVRYLSGVAFDKLALPMGLPERAYAADSETLQHTLYGGMILPVVALAGLVTAAWRGSK
DHHDDDEFDMFDHPEGGHK

Sequences:

>Translated_339_residues
MTITRRNFLATAAAGGTAAAAACATLAPAGAEARESHKVPDNAVGLLYDATLCIGCKACMSACKEANDLPLEDTIGEKLW
DTPLELSGKTYNVIKVYQSGTMENKDKAENGFAHIKRSCLHCADPSCVSACPVSAMQKRATDGVVTYNKDACIGCRYCVA
ACPFGVPQFQYDTPKPEIAKCQLCKHRMEQGKYAACAESCPTGATIFGSYAALSAEIDRRRAMKPGEPNLFPRRTPESGD
MHEKAAPEYVDYVYGQKDAGGTQVRYLSGVAFDKLALPMGLPERAYAADSETLQHTLYGGMILPVVALAGLVTAAWRGSK
DHHDDDEFDMFDHPEGGHK
>Mature_338_residues
TITRRNFLATAAAGGTAAAAACATLAPAGAEARESHKVPDNAVGLLYDATLCIGCKACMSACKEANDLPLEDTIGEKLWD
TPLELSGKTYNVIKVYQSGTMENKDKAENGFAHIKRSCLHCADPSCVSACPVSAMQKRATDGVVTYNKDACIGCRYCVAA
CPFGVPQFQYDTPKPEIAKCQLCKHRMEQGKYAACAESCPTGATIFGSYAALSAEIDRRRAMKPGEPNLFPRRTPESGDM
HEKAAPEYVDYVYGQKDAGGTQVRYLSGVAFDKLALPMGLPERAYAADSETLQHTLYGGMILPVVALAGLVTAAWRGSKD
HHDDDEFDMFDHPEGGHK

Specific function: Participates in the periplasmic electron-transferring activity of hydrogenase 2 during its catalytic turnover [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789370, Length=327, Percent_Identity=45.2599388379205, Blast_Score=297, Evalue=6e-82,
Organism=Escherichia coli, GI1790326, Length=274, Percent_Identity=34.3065693430657, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1787749, Length=295, Percent_Identity=32.5423728813559, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI2367345, Length=231, Percent_Identity=32.9004329004329, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787872, Length=184, Percent_Identity=36.9565217391304, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1787122, Length=184, Percent_Identity=36.9565217391304, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI226510944, Length=210, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787740, Length=186, Percent_Identity=30.6451612903226, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787478, Length=146, Percent_Identity=32.8767123287671, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082179, Length=112, Percent_Identity=41.0714285714286, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082180, Length=156, Percent_Identity=35.2564102564103, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788811, Length=186, Percent_Identity=31.1827956989247, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI2367245, Length=159, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787963, Length=215, Percent_Identity=23.7209302325581, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 36422; Mature: 36290

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00387 PPASE ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
5.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTITRRNFLATAAAGGTAAAAACATLAPAGAEARESHKVPDNAVGLLYDATLCIGCKACM
CCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
SACKEANDLPLEDTIGEKLWDTPLELSGKTYNVIKVYQSGTMENKDKAENGFAHIKRSCL
HHHHCCCCCCCHHHHHHHHHCCCHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
HCADPSCVSACPVSAMQKRATDGVVTYNKDACIGCRYCVAACPFGVPQFQYDTPKPEIAK
HCCCCHHHHHCCHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
CQLCKHRMEQGKYAACAESCPTGATIFGSYAALSAEIDRRRAMKPGEPNLFPRRTPESGD
HHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
MHEKAAPEYVDYVYGQKDAGGTQVRYLSGVAFDKLALPMGLPERAYAADSETLQHTLYGG
CHHHHCHHHHHHHHCCCCCCCCEEEEECCCHHHHHHCCCCCCHHHHHCCHHHHHHHHHCC
MILPVVALAGLVTAAWRGSKDHHDDDEFDMFDHPEGGHK
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TITRRNFLATAAAGGTAAAAACATLAPAGAEARESHKVPDNAVGLLYDATLCIGCKACM
CCCCCHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
SACKEANDLPLEDTIGEKLWDTPLELSGKTYNVIKVYQSGTMENKDKAENGFAHIKRSCL
HHHHCCCCCCCHHHHHHHHHCCCHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
HCADPSCVSACPVSAMQKRATDGVVTYNKDACIGCRYCVAACPFGVPQFQYDTPKPEIAK
HCCCCHHHHHCCHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
CQLCKHRMEQGKYAACAESCPTGATIFGSYAALSAEIDRRRAMKPGEPNLFPRRTPESGD
HHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
MHEKAAPEYVDYVYGQKDAGGTQVRYLSGVAFDKLALPMGLPERAYAADSETLQHTLYGG
CHHHHCHHHHHHHHCCCCCCCCEEEEECCCHHHHHHCCCCCCHHHHHCCHHHHHHHHHCC
MILPVVALAGLVTAAWRGSKDHHDDDEFDMFDHPEGGHK
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]