| Definition | Magnetospirillum magneticum AMB-1 chromosome, complete genome. |
|---|---|
| Accession | NC_007626 |
| Length | 4,967,148 |
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The map label for this gene is hybC [H]
Identifier: 83310746
GI number: 83310746
Start: 1783667
End: 1785370
Strand: Reverse
Name: hybC [H]
Synonym: amb1647
Alternate gene names: 83310746
Gene position: 1785370-1783667 (Counterclockwise)
Preceding gene: 83310747
Following gene: 83310745
Centisome position: 35.94
GC content: 65.55
Gene sequence:
>1704_bases GTGTCCAAGAGAATCACCATCGATCCCATCACCCGGATTGAAGGCCATCTGCGCATCGACGTCGAGGTCGACGGCGGCAA GGTCACCAAGGCTTGGTCGTCGGGCCAGATGTGGCGCGGCGTCGAGCTGATCCTGCTGGGCCGCGACCCGCGCGACGCCT GGGCCATCACCCAGCGCATCTGTGGCGTGTGCACCACCGTGCACGCCATGACCTCGGTCCGCGCCGTCGAGAACGCCCTC AATCTGGAAGTGCCGGTGAACGCCCAGTTCATCCGCAACATGATCATCTCGGCCCACGCCATCCATGATCACATCGTGCA CTTCTACCACCTGTCGGCGCTCGACTGGGTGGACGTGGTCAGCGCGCTGAAGGCCGATCCGGTCAAGACCGCCCAGCTGG CGGAAAGCCTGTCCGACTGGCCGCTGAACGGCCGCCACGTCATGACCCAGACCCTGGAAAAGCTGAAGACCTTCGTCGGC AGCGGCCAGCTGGGTCCGTTCACCAACGGCTATTGGGGCCATCCGGCCATGAAGCTGTCGCCGGAAGTCAACCTGCTGGC GGTGGCCCACTATCTCCAGGCGCTGGACGCCCAGCGTCACGCCAACAAGATCGTGACCCTGCTGGGCTCCAAGTCGCCGC ACATCCAGAACGTGGCGGTGGGCGGCGTGTCCAACCCCATCGCCACCGACAGCCAGTCGGTGCTGACGGTCGAGCGTCTG CTGGCCTTGAAGCCGCACATGGACGCGCTGACCGACTTCATCAACAAGGTCTATCTGGTGGACATCGCCGCCATCGGCGC CTTCTACGCCGAATGGACCAAGTACGGCGCCGGCGTCACCAACTACCTGTCGTGCCCCGACTTCCCCATGGACAGCAAGG GCACCCAGTTCCTGGTTCCCGGCGGCTACATCCCCAACGCCGATCTGGCCCAGTACAAGCCCATCACCGCCTTCGGCGAC GCTTACCTGCGCGACGGCGTCGAGGAAGGGGTCAAGCACGCCTGGTACGAATATTCCAAGCCCGGCCCGCTGCACCCCTA CAAGGGCGAGACCAAGCCCGCCTACACGGACTTCCAGGATGACGGCAAGTATTCCTGGCTGAAGGCGCCGACCTTCTACG GCAAGCGCGCCCAGGTGGGTCCGTTGGCCAGCGTCCTGGCGGGCGTCGCCGCCGGCCACCAGCCGACCATCGGCTACGCC ACCAAGGTGCTGGACACCGCCTCGTCGCTGCTGGGCTCCAAGATCCCGCTGGCGGCGCTGCACTCCACCATCGGCCGCCA TGCCGCCCGCGCCGTGCGCTCGGGCGTGATGGCCGAGGTCATGGTGCAGAACTGGCAGGCCCTGATCGACAATATCGGCA AGGGCGACACCAAGACCTTCAACAAGCCCACCTTCCCCAAGGGCGAGGTCATGGGCTACGGCTTCCACGAGGCTCCGCGC GGCCTGCTGTCCCACTGGACGGTGATCCGCGACGGCAAGATCGCCAACTACCAGTGCGTGGTGCCCTCCACCTGGAACGC CGGTCCCCGTGACGACAGCGACAATATCGGCCCCTACGAGGCCTCGCTGATGGACAACCCCATCGCCGATGCCGAACTGC CGCTGGAAGTGCTGCGCACCGTCCACTCCTTCGACCCCTGCCTGGCCTGTGCCGTGCACGTGGTCGAGGACAACAAGGAA GTGGTGCAGGTCAAGGCTCTGTAA
Upstream 100 bases:
>100_bases ATCGCCATCGAAGTGCTCGCCTATATCATTTTCGTCCGGAAATTCCCCATTCTTCCCGGCCACTCGACCCCTGCGGCCGC CGAGTAAGGAGACCTGAGCA
Downstream 100 bases:
>100_bases GTGTAAAATCCCCCTCCCGTCACCCGGCGGGAGGGGGTCTTTTTGTCTCGAGGAAGCCCATGCGCATCGTTGTTCTCGGC GTCGGCAACATCCTGCTCTC
Product: Ni,Fe-hydrogenase I large subunit
Products: NA
Alternate protein names: HYD2; Membrane-bound hydrogenase 2 large subunit; NiFe hydrogenase [H]
Number of amino acids: Translated: 567; Mature: 566
Protein sequence:
>567_residues MSKRITIDPITRIEGHLRIDVEVDGGKVTKAWSSGQMWRGVELILLGRDPRDAWAITQRICGVCTTVHAMTSVRAVENAL NLEVPVNAQFIRNMIISAHAIHDHIVHFYHLSALDWVDVVSALKADPVKTAQLAESLSDWPLNGRHVMTQTLEKLKTFVG SGQLGPFTNGYWGHPAMKLSPEVNLLAVAHYLQALDAQRHANKIVTLLGSKSPHIQNVAVGGVSNPIATDSQSVLTVERL LALKPHMDALTDFINKVYLVDIAAIGAFYAEWTKYGAGVTNYLSCPDFPMDSKGTQFLVPGGYIPNADLAQYKPITAFGD AYLRDGVEEGVKHAWYEYSKPGPLHPYKGETKPAYTDFQDDGKYSWLKAPTFYGKRAQVGPLASVLAGVAAGHQPTIGYA TKVLDTASSLLGSKIPLAALHSTIGRHAARAVRSGVMAEVMVQNWQALIDNIGKGDTKTFNKPTFPKGEVMGYGFHEAPR GLLSHWTVIRDGKIANYQCVVPSTWNAGPRDDSDNIGPYEASLMDNPIADAELPLEVLRTVHSFDPCLACAVHVVEDNKE VVQVKAL
Sequences:
>Translated_567_residues MSKRITIDPITRIEGHLRIDVEVDGGKVTKAWSSGQMWRGVELILLGRDPRDAWAITQRICGVCTTVHAMTSVRAVENAL NLEVPVNAQFIRNMIISAHAIHDHIVHFYHLSALDWVDVVSALKADPVKTAQLAESLSDWPLNGRHVMTQTLEKLKTFVG SGQLGPFTNGYWGHPAMKLSPEVNLLAVAHYLQALDAQRHANKIVTLLGSKSPHIQNVAVGGVSNPIATDSQSVLTVERL LALKPHMDALTDFINKVYLVDIAAIGAFYAEWTKYGAGVTNYLSCPDFPMDSKGTQFLVPGGYIPNADLAQYKPITAFGD AYLRDGVEEGVKHAWYEYSKPGPLHPYKGETKPAYTDFQDDGKYSWLKAPTFYGKRAQVGPLASVLAGVAAGHQPTIGYA TKVLDTASSLLGSKIPLAALHSTIGRHAARAVRSGVMAEVMVQNWQALIDNIGKGDTKTFNKPTFPKGEVMGYGFHEAPR GLLSHWTVIRDGKIANYQCVVPSTWNAGPRDDSDNIGPYEASLMDNPIADAELPLEVLRTVHSFDPCLACAVHVVEDNKE VVQVKAL >Mature_566_residues SKRITIDPITRIEGHLRIDVEVDGGKVTKAWSSGQMWRGVELILLGRDPRDAWAITQRICGVCTTVHAMTSVRAVENALN LEVPVNAQFIRNMIISAHAIHDHIVHFYHLSALDWVDVVSALKADPVKTAQLAESLSDWPLNGRHVMTQTLEKLKTFVGS GQLGPFTNGYWGHPAMKLSPEVNLLAVAHYLQALDAQRHANKIVTLLGSKSPHIQNVAVGGVSNPIATDSQSVLTVERLL ALKPHMDALTDFINKVYLVDIAAIGAFYAEWTKYGAGVTNYLSCPDFPMDSKGTQFLVPGGYIPNADLAQYKPITAFGDA YLRDGVEEGVKHAWYEYSKPGPLHPYKGETKPAYTDFQDDGKYSWLKAPTFYGKRAQVGPLASVLAGVAAGHQPTIGYAT KVLDTASSLLGSKIPLAALHSTIGRHAARAVRSGVMAEVMVQNWQALIDNIGKGDTKTFNKPTFPKGEVMGYGFHEAPRG LLSHWTVIRDGKIANYQCVVPSTWNAGPRDDSDNIGPYEASLMDNPIADAELPLEVLRTVHSFDPCLACAVHVVEDNKEV VQVKAL
Specific function: This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake [H]
COG id: COG0374
COG function: function code C; Ni,Fe-hydrogenase I large subunit
Gene ontology:
Cell location: Cell membrane; Peripheral membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]
Homologues:
Organism=Escherichia coli, GI1789368, Length=569, Percent_Identity=59.2267135325132, Blast_Score=717, Evalue=0.0, Organism=Escherichia coli, GI1787207, Length=591, Percent_Identity=43.3164128595601, Blast_Score=470, Evalue=1e-134,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001501 - InterPro: IPR018194 [H]
Pfam domain/function: PF00374 NiFeSe_Hases [H]
EC number: =1.12.99.6 [H]
Molecular weight: Translated: 61882; Mature: 61751
Theoretical pI: Translated: 7.12; Mature: 7.12
Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 2.1 %Met (Translated Protein) 3.2 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 1.9 %Met (Mature Protein) 3.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSKRITIDPITRIEGHLRIDVEVDGGKVTKAWSSGQMWRGVELILLGRDPRDAWAITQRI CCCCEEECCHHCCCCEEEEEEEECCCEEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHH CGVCTTVHAMTSVRAVENALNLEVPVNAQFIRNMIISAHAIHDHIVHFYHLSALDWVDVV HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH SALKADPVKTAQLAESLSDWPLNGRHVMTQTLEKLKTFVGSGQLGPFTNGYWGHPAMKLS HHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEC PEVNLLAVAHYLQALDAQRHANKIVTLLGSKSPHIQNVAVGGVSNPIATDSQSVLTVERL CCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEECCCCCCCCCCCHHHHHHHHH LALKPHMDALTDFINKVYLVDIAAIGAFYAEWTKYGAGVTNYLSCPDFPMDSKGTQFLVP HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCEEEEC GGYIPNADLAQYKPITAFGDAYLRDGVEEGVKHAWYEYSKPGPLHPYKGETKPAYTDFQD CCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC DGKYSWLKAPTFYGKRAQVGPLASVLAGVAAGHQPTIGYATKVLDTASSLLGSKIPLAAL CCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHH HSTIGRHAARAVRSGVMAEVMVQNWQALIDNIGKGDTKTFNKPTFPKGEVMGYGFHEAPR HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCHHHHHH GLLSHWTVIRDGKIANYQCVVPSTWNAGPRDDSDNIGPYEASLMDNPIADAELPLEVLRT HHHHHCEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHH VHSFDPCLACAVHVVEDNKEVVQVKAL HHCCCHHHHHHHHHEECCCEEEEEECC >Mature Secondary Structure SKRITIDPITRIEGHLRIDVEVDGGKVTKAWSSGQMWRGVELILLGRDPRDAWAITQRI CCCEEECCHHCCCCEEEEEEEECCCEEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHH CGVCTTVHAMTSVRAVENALNLEVPVNAQFIRNMIISAHAIHDHIVHFYHLSALDWVDVV HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH SALKADPVKTAQLAESLSDWPLNGRHVMTQTLEKLKTFVGSGQLGPFTNGYWGHPAMKLS HHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEC PEVNLLAVAHYLQALDAQRHANKIVTLLGSKSPHIQNVAVGGVSNPIATDSQSVLTVERL CCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEECCCCCCCCCCCHHHHHHHHH LALKPHMDALTDFINKVYLVDIAAIGAFYAEWTKYGAGVTNYLSCPDFPMDSKGTQFLVP HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCEEEEC GGYIPNADLAQYKPITAFGDAYLRDGVEEGVKHAWYEYSKPGPLHPYKGETKPAYTDFQD CCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC DGKYSWLKAPTFYGKRAQVGPLASVLAGVAAGHQPTIGYATKVLDTASSLLGSKIPLAAL CCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHH HSTIGRHAARAVRSGVMAEVMVQNWQALIDNIGKGDTKTFNKPTFPKGEVMGYGFHEAPR HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCHHHHHH GLLSHWTVIRDGKIANYQCVVPSTWNAGPRDDSDNIGPYEASLMDNPIADAELPLEVLRT HHHHHCEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHH VHSFDPCLACAVHVVEDNKEVVQVKAL HHCCCHHHHHHHHHEECCCEEEEEECC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796 [H]