Click here to switch to the map view.

The map label for this gene is hybD [H]

Identifier: 83310745

GI number: 83310745

Start: 1783125

End: 1783607

Strand: Reverse

Name: hybD [H]

Synonym: amb1646

Alternate gene names: 83310745

Gene position: 1783607-1783125 (Counterclockwise)

Preceding gene: 83310746

Following gene: 83310744

Centisome position: 35.91

GC content: 64.8

Gene sequence:

>483_bases
ATGCGCATCGTTGTTCTCGGCGTCGGCAACATCCTGCTCTCGGACGAGGGAATCGGCGTTCACGCCGTCACCCGGCTGGA
AGAGCTTTACGATCTCCCCGCGGAGGTCGAAGTCATCGACGGCGGCACCTCGGGCATGGATTGCCTGGATCAGGTGTCCG
AGGCCGATCACCTGATCATCGCCGACTGCATGCGCTCGAAGAAGGAGCCGGGGACCATCACCCGGTTGACCGGCGACCAG
ATTCCCGCCTTCTTCAAGACCCGCATCTCGCCCCATCAGGTCGGCCTGTCCGACATGCTGGGCGCTTTGAATTTCCATGG
CCTGATGCCCAAGAACATCGTGCTGTTCGGGGTCGAGCCCCTGTCCTTCGACCTGTCCATGGAGCCCACGCCCACCGTGG
CCGCCCGCCTGCCTGAGCTGGTGGAGCGGATCGTCGGTGAACTGGCCGCCATCGGCGTCGCAGTCGGCCGGAAGGCCGCC
TGA

Upstream 100 bases:

>100_bases
TCGAGGACAACAAGGAAGTGGTGCAGGTCAAGGCTCTGTAAGTGTAAAATCCCCCTCCCGTCACCCGGCGGGAGGGGGTC
TTTTTGTCTCGAGGAAGCCC

Downstream 100 bases:

>100_bases
CCATGTGCATGGCCGTGCCGTCGCGGATCACCGCCATCGACAACGGCGTTGCCACGGTCGAGGCCTTCGGGCAGTCGCGC
ACCACCAGCCTGATGCTGCT

Product: Ni,Fe-hydrogenase maturation factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MRIVVLGVGNILLSDEGIGVHAVTRLEELYDLPAEVEVIDGGTSGMDCLDQVSEADHLIIADCMRSKKEPGTITRLTGDQ
IPAFFKTRISPHQVGLSDMLGALNFHGLMPKNIVLFGVEPLSFDLSMEPTPTVAARLPELVERIVGELAAIGVAVGRKAA

Sequences:

>Translated_160_residues
MRIVVLGVGNILLSDEGIGVHAVTRLEELYDLPAEVEVIDGGTSGMDCLDQVSEADHLIIADCMRSKKEPGTITRLTGDQ
IPAFFKTRISPHQVGLSDMLGALNFHGLMPKNIVLFGVEPLSFDLSMEPTPTVAARLPELVERIVGELAAIGVAVGRKAA
>Mature_160_residues
MRIVVLGVGNILLSDEGIGVHAVTRLEELYDLPAEVEVIDGGTSGMDCLDQVSEADHLIIADCMRSKKEPGTITRLTGDQ
IPAFFKTRISPHQVGLSDMLGALNFHGLMPKNIVLFGVEPLSFDLSMEPTPTVAARLPELVERIVGELAAIGVAVGRKAA

Specific function: Protease involved in the C-terminal processing of hybC, the large subunit of hydrogenase 2. Specifically cleaves off a 15 amino acid peptide from the C-terminus of the precursor of hybC [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1789367, Length=161, Percent_Identity=50.3105590062112, Blast_Score=145, Evalue=1e-36,
Organism=Escherichia coli, GI1787209, Length=124, Percent_Identity=40.3225806451613, Blast_Score=103, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 17087; Mature: 17087

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIVVLGVGNILLSDEGIGVHAVTRLEELYDLPAEVEVIDGGTSGMDCLDQVSEADHLII
CEEEEEECCCEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCEEE
ADCMRSKKEPGTITRLTGDQIPAFFKTRISPHQVGLSDMLGALNFHGLMPKNIVLFGVEP
HHHHHCCCCCCCEEEECCCCCCHHHHHCCCHHHCCHHHHHHHHHCCCCCCCCEEEEECCC
LSFDLSMEPTPTVAARLPELVERIVGELAAIGVAVGRKAA
CEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRIVVLGVGNILLSDEGIGVHAVTRLEELYDLPAEVEVIDGGTSGMDCLDQVSEADHLII
CEEEEEECCCEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCEEE
ADCMRSKKEPGTITRLTGDQIPAFFKTRISPHQVGLSDMLGALNFHGLMPKNIVLFGVEP
HHHHHCCCCCCCEEEECCCCCCHHHHHCCCHHHCCHHHHHHHHHCCCCCCCCEEEEECCC
LSFDLSMEPTPTVAARLPELVERIVGELAAIGVAVGRKAA
CEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8021226; 9278503; 10331925 [H]