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The map label for this gene is hypF [H]

Identifier: 83310740

GI number: 83310740

Start: 1779438

End: 1780514

Strand: Reverse

Name: hypF [H]

Synonym: amb1641

Alternate gene names: 83310740

Gene position: 1780514-1779438 (Counterclockwise)

Preceding gene: 83310741

Following gene: 83310739

Centisome position: 35.85

GC content: 70.19

Gene sequence:

>1077_bases
GTGACCTCGGCCATCGCCCAGATCGTCGCCCTGCCCCGCGCCGTGCCGCCGGTGCTGGCCGTCGGCGCCTTTCTTAAGAA
TGCCGTCTGCGTGACCAGGGGGGCCGAGGCGCTGATCTCGGCCAATCACGGCGATCTCGGCACCCCCGAAGCCATCGCCG
CCTTCGAAGCCTGCGTTGAGGCCCTGGTGGCCGAAGCGGGCGTCACCCCGGTGCGGGTCGCCCACGACCTGCATCCCGAT
TTCCACTGTACCCGCTATGCCCAATCCCTGGACCTGCCCCAGGTGCCGGTCCAGCACCACCATGCCCACGCCGCCGCCAT
CGCCGCCGAGCACGGCATCGAAGGCCCGGTGATCGGCGTCTCCTTCGACGGCTTCGGCCTGGGGCCGGGCAACCAGTCCT
GGGGGGGGGAATTGCTGCTGGCCGAGGGCGCCCATTACCGCCGCCTGGGGCATCTGGCCCTTTTGCCTCAGCCGGGCGGC
GACCGCGCGGCACGCGAGCCCTGGCGCATGGCGGCCAGCGTCCTGCACAAGCTGGGGCGCGGACCTGAAATCGCCGAGCG
CTTCAAGGACATCCCCGCCGCCGCCATGCTGGGGCAGATGCTGGACCGGCGGCTCAACAGCCCCGAGACGTCGTCGGCCG
GACGGCTGTTCGACGCCGCCTGCGGCCTTTTGGGCCTGCATCCCATCGCCGAATTCGAGAGTCAGGCCCCCATGGCGCTG
GAAGCCCTGGTGACGGCGCCCGAGGTGCTGCCCACCGGCTGGATTCTCCAGGACGGTGTTCTGGACCTTTTGCCGCTGCT
GGCCTGCCTTGCCCATTGCACCGACCCGGTACGCGGTGCCAACCTGTTCCACGGCACCCTGATCGCCGCCATGGCCAAGT
GGATCGGCGACGTGGCCGCCACCGCCGGCACGCCCTATGTCACCTTGGGAGGAGGCTGCTTCCTCAACAAGGTGCTGACC
GGCGGTCTGGTGGACAGGCTGACGGCCTTGGGGCTCAGGCCATTGACCGCCGCGAAGGTATCGCCGGGCGATGCCGGCCT
GTCCCTGGGGCAGGCCTGGATCGCCGCGTTGAGTTGA

Upstream 100 bases:

>100_bases
GGCTGGCCTGGATCAACGAGGGCCGCAAGCGCATGGTGACGGAGCGCATCCGCCGCCTGGAAGCCAAGCTGGCCGCCGCC
AAGGCGAGTCTGGAGGGTAA

Downstream 100 bases:

>100_bases
GTATCAGAGGAACCAACACCATGTGCCTTGCCCTCCCCGCCCTGGTCACCGAGCTGTTGCCCGACGATTCGGCCAAGGTC
AATCTCGGCGGCGTCGCCAA

Product: hydrogenase maturation protein hypF1

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MTSAIAQIVALPRAVPPVLAVGAFLKNAVCVTRGAEALISANHGDLGTPEAIAAFEACVEALVAEAGVTPVRVAHDLHPD
FHCTRYAQSLDLPQVPVQHHHAHAAAIAAEHGIEGPVIGVSFDGFGLGPGNQSWGGELLLAEGAHYRRLGHLALLPQPGG
DRAAREPWRMAASVLHKLGRGPEIAERFKDIPAAAMLGQMLDRRLNSPETSSAGRLFDAACGLLGLHPIAEFESQAPMAL
EALVTAPEVLPTGWILQDGVLDLLPLLACLAHCTDPVRGANLFHGTLIAAMAKWIGDVAATAGTPYVTLGGGCFLNKVLT
GGLVDRLTALGLRPLTAAKVSPGDAGLSLGQAWIAALS

Sequences:

>Translated_358_residues
MTSAIAQIVALPRAVPPVLAVGAFLKNAVCVTRGAEALISANHGDLGTPEAIAAFEACVEALVAEAGVTPVRVAHDLHPD
FHCTRYAQSLDLPQVPVQHHHAHAAAIAAEHGIEGPVIGVSFDGFGLGPGNQSWGGELLLAEGAHYRRLGHLALLPQPGG
DRAAREPWRMAASVLHKLGRGPEIAERFKDIPAAAMLGQMLDRRLNSPETSSAGRLFDAACGLLGLHPIAEFESQAPMAL
EALVTAPEVLPTGWILQDGVLDLLPLLACLAHCTDPVRGANLFHGTLIAAMAKWIGDVAATAGTPYVTLGGGCFLNKVLT
GGLVDRLTALGLRPLTAAKVSPGDAGLSLGQAWIAALS
>Mature_357_residues
TSAIAQIVALPRAVPPVLAVGAFLKNAVCVTRGAEALISANHGDLGTPEAIAAFEACVEALVAEAGVTPVRVAHDLHPDF
HCTRYAQSLDLPQVPVQHHHAHAAAIAAEHGIEGPVIGVSFDGFGLGPGNQSWGGELLLAEGAHYRRLGHLALLPQPGGD
RAAREPWRMAASVLHKLGRGPEIAERFKDIPAAAMLGQMLDRRLNSPETSSAGRLFDAACGLLGLHPIAEFESQAPMALE
ALVTAPEVLPTGWILQDGVLDLLPLLACLAHCTDPVRGANLFHGTLIAAMAKWIGDVAATAGTPYVTLGGGCFLNKVLTG
GLVDRLTALGLRPLTAAKVSPGDAGLSLGQAWIAALS

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=367, Percent_Identity=35.4223433242507, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 37063; Mature: 36932

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSAIAQIVALPRAVPPVLAVGAFLKNAVCVTRGAEALISANHGDLGTPEAIAAFEACVE
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHEECCCCCCCCHHHHHHHHHHHH
ALVAEAGVTPVRVAHDLHPDFHCTRYAQSLDLPQVPVQHHHAHAAAIAAEHGIEGPVIGV
HHHHHCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEE
SFDGFGLGPGNQSWGGELLLAEGAHYRRLGHLALLPQPGGDRAAREPWRMAASVLHKLGR
EECCCCCCCCCCCCCCCEEEECCCHHHHHCCEEECCCCCCCCHHHHHHHHHHHHHHHHCC
GPEIAERFKDIPAAAMLGQMLDRRLNSPETSSAGRLFDAACGLLGLHPIAEFESQAPMAL
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCHHH
EALVTAPEVLPTGWILQDGVLDLLPLLACLAHCTDPVRGANLFHGTLIAAMAKWIGDVAA
HHHHHCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TAGTPYVTLGGGCFLNKVLTGGLVDRLTALGLRPLTAAKVSPGDAGLSLGQAWIAALS
HCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSAIAQIVALPRAVPPVLAVGAFLKNAVCVTRGAEALISANHGDLGTPEAIAAFEACVE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHEECCCCCCCCHHHHHHHHHHHH
ALVAEAGVTPVRVAHDLHPDFHCTRYAQSLDLPQVPVQHHHAHAAAIAAEHGIEGPVIGV
HHHHHCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEE
SFDGFGLGPGNQSWGGELLLAEGAHYRRLGHLALLPQPGGDRAAREPWRMAASVLHKLGR
EECCCCCCCCCCCCCCCEEEECCCHHHHHCCEEECCCCCCCCHHHHHHHHHHHHHHHHCC
GPEIAERFKDIPAAAMLGQMLDRRLNSPETSSAGRLFDAACGLLGLHPIAEFESQAPMAL
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCHHH
EALVTAPEVLPTGWILQDGVLDLLPLLACLAHCTDPVRGANLFHGTLIAAMAKWIGDVAA
HHHHHCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TAGTPYVTLGGGCFLNKVLTGGLVDRLTALGLRPLTAAKVSPGDAGLSLGQAWIAALS
HCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8486288 [H]